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CategoryMiSeqDNBSEQ-G99DNBSEQ-G99
[Superior (green) / Inferior (red)]
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Sequencing principleUses clonal bridge amplication to form DNA clusters on flow cells with pre-attached short oligos
1. Prone to PCR bias and error accumulation.
2. Increased duplication reads, arising from optical duplicates (a common problem associated with randomly clustered flow cells), and complementary strands of the same library generating independent clusters.
3. Miseq single index misassignment rates range from 0.1-0.5%. ExAmp single index misassignment rates range from 1-6%. (Note that ExAmp is only used on HiSeq 3000/4000/X)		Uses DNBSEQ™ technology - DNA Nanoballs (DNB) are formed by rolling circle amplification, and adhered to a patterned array flow cell for sequencing.
1. Amplification is based on original template. This results in linear amplification with low amplification bias and low error accumulation.
2. Low duplication rates due to patterned array configuration. Also, only one strand (the sense strand) is used for circularisation, hence duplication rates on MGI is far lower than other platforms, increasing the read efficiency, and reducing data wastage.
3. Without accumulation of PCR errors, index hopping rate can be reduced to 0.0001％ to 0.0004％ using a single index.		DNBSEQ™ excels in terms of low bias, low duplication rates, and low index hopping rates.
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Parameters and performanceRelease yearInstrument - 2011
V3 - 2013		2022
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Chip(s)/Run12Able to run 2 flow cells simultaneously, achieving greater flexibility.
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Lane(s)/Chip11
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Effective readsv2 nano-1M
v2 micro-4M
v2-15M
v3-25M		80MMiseq flow cells have a larger throughput range.
G99 has higher throughput.
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Read lengthsSE36, PE25, PE75, PE150, PE250, PE300SE100/PE50, SE300/PE150, PE300, SE400Longer PE300 and SE400 read lengths are planned for release (in 2023), these can be used for forensics applications.
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Chip technologyRandomly clustered oligos, optical interference tends to occur between clusters, and chip productivity tends to be low. Patterned array configuration, can achieve higher loading of DNBs, low optical duplication, and higher chip productivity.Patterned array can increase chip productivity and reduce optical duplication.
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TAT15M PE25 ~5.5 h
15M PE150 ~24 h
15M PE250 ~39 h
25M PE75 ~21 h
25M PE300 ~56 h
4M PE150 ~19 h
1M PE150 ~17 h
1M PE250 ~28 h		SE50 ~3 h
SE100 ~5 h
PE150 ~12 h		Higher throughput with lower TAT required.
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Q30v2 PE25 ＞90%
v2 PE150 ＞80%
v2 PE250 ＞75%
v3 PE75 ＞85%
v3 PE300 ＞70% 		SE100/PE50 >90%
PE150 >85%
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Max data outputnano (PE250) 500 Mb
micro (PE150) 1.2Gb
v2 (PE250) 7.5-8.5Gb
v3 (PE300) 13.2-15Gb		Output range is 8~96G：
2*8G（SE100/PE50）
2*24Gb（SE300/PE150）
2*48G（PE300）
2*32G（SE400）
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Sequencing channel(s)4-color4-color
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Operating system and useStorage1TB6TB
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Operating SystemWindows 10Windows 10
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Operating environment - Altitude<2000 m<3000 mHigher altitude compatibility, especially in locations situated between 2000-3000m
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PriceReagent30% off prices:
25M PE75 9114RMB (1302USD), per Gb 2398RMB (350USD)
25M PE300 15925RMB (2275USD), per Gb 1061RMB (155USD)		PE150 sequencing reagent set ~4800RMB (650 USD), per Gb ~200RMB (27 USD)More cost-effective reagent kits for end-users
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InstrumentUS catalog price: 128,000USD
Final closing price (China): ~1,780,000RMB (260,000USD)		Estimated selling price ~990,000RMB (~129,000 USD)Slightly more expensive than MiSeq
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Can be packaged with MGISP-100/DNBelab D sample prep automators, or in-built with a bioinformatics module for secondary analysis. Cheaper than MiSeq in domestic China
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Applications and solutionsWorkflowThird-party solutions required for sample prep automation, all MiSeqs come with the MiSeq Reporter built-in data analysis software.Integrated automated sample prep workflow
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ApplicationsKey applications:
Small genome, targeted gene (amplicon or capture), 16s metagenomics, forensic genomics
Secondary applications:
Small RNA-Seq, gene expression, WGS (small genome), epigenome protein-DNA interactions		Key applications:
Oncology small panels, small genome, methylation analysis, forensics identification
Secondary applications:
Low-pass WGS, RNA sequencing
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Integrated end-to-end solutionNo complete solutions at presentLibrary prep: MGISP-100 or DNBelab D sample prep automators
Bioinformatics: In-built 1U server, 32TB hard disk+256GB RAM, pre-installed MetargetCOVID, MGAP, PFI and other bioinformatics pipelines. Can complete secondary analysis directly after attaining sequencing results, analysis time <2h.
Overall management: ZLIMS system for entire laboratory life cycle management of sample extraction, library prep, sequencing, bioinformatics analysis and reporting.		Total solution from sample to report
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SupportProduction siteU.S.China
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Technical support and after-salesGlobally distributed service centers, limited presence in domestic China.Globally distributed service centers, including in the U.S., EU, APAC, Japan, Oceania, and China.Strong technical and after-sales support globally
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IVD certificationFDA, NMPA, CE-IVDNMPA，CE-IVDMiSeq has FDA approval in addition to NMPA and CE-IVD
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