2012_09_20 Gibson Amanda's T7 construct
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
View only
 
 
Still loading...
ABCDEFGHIJKLMNOPQRST
1
PCR wellpieceforward primerreverse primerT_anneal% DMSO
2
1backbone 1125126700
3
2125126705
4
31251267010
5
4125126720
6
5125126725
7
61251267210
8
9
7backbone 2131132700
10
8131132705
11
91311327010
12
10131132720
13
11131132725
14
121311327210
15
16
13ADH127128700
17
14127128705
18
151271287010
19
16127128720
20
17127128725
21
181271287210
22
23
19FLS129130700
24
20129130705
25
211291307010
26
22129130720
27
23129130725
28
241291307210
29
30
Do 25 uL/well in case any of them work
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Loading...
 
 
 
9_20 first PCR
9_22 2nd PCR -make pieces
9_23 Gibson Assemblies
9_25 PCR
9_26
9_27
9_27-2
9_28
10/1
10/2
10/3
10/4
10/5
10/9
10/10
10/11
10/12
Copy of 10/12
10/13
10/14
10/15
10/16
Copy of 10/16
PCR products, gibson assemblies, etc.
10/22-26
10/29
BCA
Protein for Gels
Sheet31
Sheet34