202300816-cgig-polyIC-cDNA-calcs

	A	B	C	D	E	F	G	H	I	J	K	L
1	sample_ID	Dnased_RNA(ng/uL)	RNA_in_rxn(ng)	vol_to_400ng(uL)	vol_dT_primers_1:10_dilution(uL)	primer_denature_vol(uL)	vol_h2o_to_15uL(uL)	vol_5x_rxn_buffer(uL)	vol_10mM_dNTPs(uL)	vol_MMLV(uL)	vol_h2o_to_25uL(uL)	vol_final(uL)

2	D4	370	400	1.08	1	15	12.92	5	1.25	0.4	3.35	25
3	D9	520	400	0.77	1	15	13.23	5	1.25	0.4	3.35	25
4	D12	155	400	2.58	1	15	11.42	5	1.25	0.4	3.35	25
5	D14	474	400	0.84	1	15	13.16	5	1.25	0.4	3.35	25
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9	MMLV_master_mix	vol_single rxn	number of reactions	vol_total(uL)
10	5x_rxn_buffer	5	5	25
11	10mM_dNTPs	1.25	5	6.25
12	H2O	3.35	5	16.75
13	MMLV	0.4	5	2
14	SUM	10
15				Add 10uL to each sample.
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