NovaSeq_Pooling.xlsx

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V
1	NovaSeq Sample Sign Up
2	Abbreviations: SE= single-end; PE= paired-end; M= million
3	If you are unsure how to fill a column, leave blank and we will contact you for consultation.
4	NovaSeq now uses the reverse complement for the i5 index. Tab 2 has some common index sequences: Tab3 has Core lab contact information
5	User Name	PI, if not user	Sample Type	ProjectName	Preferred run method	Alternative acceptable methods	Reads needed (Total Project)	Expected Library Completion	sampleID	I7_Index_ID	IndexSequence	I5_Index_ID	IndexSequence	SingleIndexID	IndexSequence	Avg Frag Size	KAPA	Volume (ul)	Run Schedule (Estimated)	Pooled Samples?	Contact Information	Comments / Special Considerations

6	Example1		HiC	HiC	50PE	Any PE	30M	Done	HiC2	D701	ATTACTCG	D501	AGGCTATA			600	12nM	20 ul	TSL fill	No	cynthia.vied@med.fsu.edu	Will run on any flowcell for faster turnaround
7	Example2		RNA-Seq	TBI	100SE	Any	100M	07/21/2018	TBI1					Try not to use single indices	GGCTAC	350	10nM	15 ul	Mid July	No		Need lane sharing: RNA-Seq
8	New NovaSeq chemistry uses reverse complement for the i5 primer (not i7) Use the complement tool if help is needed: https://www.bioinformatics.org/sms/rev_comp.html
9				No Characters or spaces					No Characters or spaces	No Characters or spaces		No Characters or spaces		No Characters or spaces
10
11	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_IgGA_5mpi	D706	GAATTCGT	D501	AGGCTATA
12	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_IgGB_15mpi	D707	CTGAAGCT	D501	AGGCTATA
13	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_IgGC_30mpi	D708	TAATGCGC	D501	AGGCTATA
14	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_5mpiA	D709	CGGCTATG	D501	AGGCTATA
15	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_5mpiB	D710	TCCGCGAA	D501	AGGCTATA
16	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_5mpiC	D711	TCTCGCGC	D501	AGGCTATA
17	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_15mpiA	D712	AGCGATAG	D501	AGGCTATA
18	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_15mpiB	D702	TCCGGAGA	D502	GCCTCTAT
19	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_15mpiC	D703	CGCTCATT	D502	GCCTCTAT
20	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_30mpiA	D704	GAGATTCC	D502	GCCTCTAT
21	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_30mpiB	D705	ATTCAGAA	D502	GCCTCTAT
22	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		vRNAP_30mpiC	D710	TCCGCGAA	D502	GCCTCTAT
23	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_IgGA_5mpi	D711	TCTCGCGC	D502	GCCTCTAT
24	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_IgGB_15mpi	D712	AGCGATAG	D502	GCCTCTAT
25	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_IgGC_30mpi	D702	TCCGGAGA	D507	ACGTCCTG
26	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_5mpiB	D704	GAGATTCC	D507	ACGTCCTG
27	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_5mpiC	D705	ATTCAGAA	D507	ACGTCCTG
28	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_15mpiB	D702	TCCGGAGA	D508	GTCAGTAC
29	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_15mpiC	D703	CGCTCATT	D508	GTCAGTAC
30	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_30mpiB	D705	ATTCAGAA	D508	GTCAGTAC
31	Villa_lab		ChIP-seq	pool3_RNAP_binding	100SE, 50 PEor higher		20M		nvRNAP_60mpiC	D706	GAATTCGT	D508	GTCAGTAC
32
33	Jane	Dennis	MOAseq	MOA_pool_su24	PE50 or greater	any PE	550M	complete	MOA_10A_scrI	7-447	CCTACGGG	5-399	AGCTGTCA
34									MOA_10A_scrII	7-448	TGCTATAT	5-400	GGGTGATG
35									MOA_CA_scr5	7-449	AACCGAAC	5-401	TACTCCAG
36									MOA_CA_scr8	7-450	ACCTGCTT	5-402	CCCGGATC
37									MOA_CA_A3	7-451	CCCATGCG	5-403	CAGGTGTT
38									MOA_CA_A11	7-473	TCCCAGCC	5-425	GGCAGTTC
39									MOA_10A_A5	7-453	TAGACAAT	5-405	TTTCCTGT
40									MOA_10A_A6	7-454	TTCTTCCT	5-406	CAGAGCAC
41									MOA_10A_AE	7-455	CACCTAAA	5-407	CCAGGTAC
42									MOA_10A_AE2	7-463	ATCGTCTC	5-415	CAGGCGAA
43									MOA_10A_B9	7-456	GTACTCGC	5-408	TTTGCGCT
44									MOA_10A_B92	7-464	CCTTGTGA	5-416	GGAGCTAG
45									MOA_10A_B15	7-457	TAACCAGT	5-409	AGGGCGTT
46									MOA_10A_BD	7-458	CGGAAACT	5-410	CCGTACGC
47									MOA_10A_BD2	7-466	CAAATTCT	5-418	CCAACATT
48									MOA_CA_B12	7-467	CTCCTCAC	5-419	TATCCGAT
49									MOA_CA_B21	7-468	ATGTGCAA	5-420	GTCACTAT
50									MOA_CA_Z7	7-474	CTATTCGT	5-426	ACAATGCT
51									MOA_CA_Z23	7-470	ACGCATGG	5-422	AATTGACT
52									MOA_10A_ZA	7-471	ACACCACC	5-423	AGCGTATT
53									MOA_10A_Z4	7-472	CACGCTGA	5-424	ACGAAGTT
54	Jane	Dennis	MNase-seq	genomic_pool_su24	PE50 or greater	any PE	1B	complete
55									genomic_10A_scr1_5U	7-475	GAGCCATT	5-427	TCCTGGTC
56									genomic_10A_scr1_200U	7-476	ATTAATCG	5-428	AATGCTGT
57									genomic_10A_scr2_5U	7-477	GAAGGAGC	5-429	TGCAGAAC
58									genomic_10A_scr2_200U	7-478	GATTACAA	5-430	CCGGACAG
59	Jane	Dennis	ChIP	ChIP_pool2_su24	PE50 or greater	any PE	850M	complete
60									CA1a_shHP1a_3_5U_H2AZ	7-399	TGACAGCT	5-447	CCCGTAGG
61									CA1a_shHP1a_3_200U_H2AZ	7-400	CATCACCC	5-448	ATATAGCA
62									CA1a_shHP1a_11_5U_H2AZ	7-401	CTGGAGTA	5-449	GTTCGGTT
63									CA1a_shHP1a_11_200U_H2AZ	7-402	GATCCGGG	5-450	AAGCAGGT
64									CA1a_shHP1b_12_5U_H2AZ	7-403	AACACCTG	5-451	CGCATGGG
65									CA1a_shHP1b_12_200U_H2AZ	7-404	GTGACGTT	5-452	TCCCAGAT
66									CA1a_shHP1b_21_5U_H2AZ	7-405	ACAGGAAA	5-453	ATTGTCTA
67									CA1a_shHP1b_21_200U_H2AZ	7-406	GTGCTCTG	5-454	AGGAAGAA
68									CA1a_shH2AZ_7_5U_HP1a	7-407	GTACCTGG	5-455	TTTAGGTG
69									CA1a_shH2AZ_7_200U_HP1a	7-408	AGCGCAAA	5-456	GCGAGTAC
70									CA1a_shH2AZ_7_5U_HP1b	7-409	AACGCCCT	5-457	ACTGGTTA
71									CA1a_shH2AZ_7_200U_HP1b	7-410	GCGTACGG	5-458	AGTTTCCG
72									CA1a_shH2AZ_23_5U_HP1a	7-411	GCCAGATT	5-459	TTCTCAGC
73									CA1a_shH2AZ_23_200U_HP1b	7-412	ATAGCAGA	5-460	TAAGACAT
74									CA1a_shH2AZ_23_5U_HP1b	7-413	GAGATGAT	5-461	AGGCGTGA
75									CA1a_shH2AZ_23_200U_HP1b	7-414	GCCCGTCT	5-462	GCTGTTGC
76									10A_shH2AZ_4_5U_HP1a	7-418	AATGTTGG	5-466	AGAATTTG
77									10A_shH2AZ_4_200U_HP1a	7-419	ATCGGATA	5-467	GTGAGGAG
78									10A_shH2AZ_1A_200U_HP1b	7-420	ATAGTGAC	5-468	TTGCACAT
79									10A_shHP1b_D_5U_H2AZ	7-421	GCTCCCTG	5-469	ACATTTGC
80									10A_shHP1b_15_200U_H2AZ	7-422	AGTCAATT	5-470	CCATGCGT
81									10A_BD_5U_IgG	7-432	TTCGCTCA	5-480	TGGCAAGT
82									10A_BD_200U_IgG	7-433	TCTTAGTT	5-481	CAATTCCT
83									10A_BD_5U_H2AZ	7-434	ACCACTCG	5-482	TGTAATAG
84									10A_BD_200U_H2AZ	7-435	AGAACGAT	5-483	GGTCCCTA
85									10A_shZ1A_5U_HP1a	7-436	ATCCCGTA	5-484	GGTCTTCT
86									10A_shZ1A_200U_HP1a	7-437	GCTGGACG	5-485	CTGTTACG
87									10A_shZ1A_5U_HP1b	7-438	TGATTGAT	5-486	TTATGGGT
88									10A_A5_5U_H2AZ	7-439	CCCAAATG	5-487	CAATGGTG
89									10A_A5_200U_H2AZ	7-440	ATTCGCAT	5-488	ATAGCTAA
90									10A_A6_5U_H2AZ	7-441	CGTCAAGA	5-489	GGGTTGTT
91									10A_A6_200U_H2AZ	7-442	CTTCGACC	5-490	ACAGTAAC
92									10A_AE_5U_H2AZ	7-443	CAGACGAC	5-491	GTGCTCGG
93									10A_AE_200U_H2AZ	7-444	ATCGTAGG	5-396	TCGAAACT
94									CA_A11_5U_IgG	7-445	GCGCAGAC	5-397	AGAGGTTC
95									CA_A11_200U_IgG	7-446	AATTGCGG	5-398	CACTGGGC
96
97	Jane	Dennis	MNase-seq	sensitivity_pool_su24	PE50 or greater	any PE	750M	complete	sensitivity_10A_AE_5	7-239	CGTGGATT	5-287	CTAAGAGT
98									sensitivity_10A_AE_200	7-240	GTAGATGC	5-288	TGTTGCTC
99									sensitivity_10A_A6_5	7-241	TACCGCTC	5-289	CGTCACTG
100									sensitivity_10A_A6_200	7-242	CGAACCAC	5-290	TCAATCTT