ABCDEFGHIJKLMNOPQRSTUVWXYZ
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n-tMade DateMade ByDescriptionCloning SitesVectorResistance
Concentration (ng/uL)
Comments
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PG15/26/2015PinarYeast cofilin from Cristian Suarez, Kovar/Blanchoin lab. D34C mutationpGEX
amp/carbenicillin
GST-tagged bacterial expression construct. a few extra copies in Pinar's box. D34C mutation for labeling purposes
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PG26/3/2015PinarCapping protein from Higgs lab a1b2 (mouse); originally from John Cooper, originally from Pekka LappelaienenpRSF Duet 1Kan6His-tagged bacterial expression construct
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PG36/3/2015Pinar"" a2b2pRSF Duet 1Kan""
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PG47/7/2015Pinarwt FMN2 full length GFP from Colleen SkauAmp/Neomycin1ug/uL
Full length GFP FMN2 in some strange T7 vector from Colleen Skau - likely M98 from GeneCopoeia, w/ C-term eGFP in CMV promoter
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PG57/28/2015Pinarwt FMN2 FH1-FH2, pcr from PG4 into LIC vectorAddgene 29718, pET His6 StrepII LICAmpHis, strep, TEV cleavable bacterial expression construct, T7 promoter
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PG69/16/2016Pinarwt FMN2 FH1-FH2truncated, PCR from PG4 into LIC vector using FH1truncation primers. aa1021-1529, 7 polyPsAddgene 29718, pET His6 StrepII LICAmp30.2ng/uLSame as PG5, but truncated FH1 domain with 7 polyPs
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PG79/16/2016PinarFMN1 FH1-FH2truncated I-->A mutant, PCR from colleens wt FL into LIC vector using FH1 truncation primers, aa1010-1529, 8 polyPsAddgene 29718, pET His6 StrepII LICAmp23.8ng/uLI-->A mutant, same as PG5 but truncated FH1 domain with 8 PolyPs
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PG89/16/2016PinarFMN2 FH1-FH2truncated I-->A mutant, PCR from colleens wt FL into LIC vector using FH1 truncation primers, aa988-1529, 10polyPsAddgene 29718, pET His6 StrepII LICAmp37.8ng/uLI-->A mutant, same as PG5 but truncated FH1 domain with10 PolyPs
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PG99/16/2016PinarFMN2 FH1-FH2truncated I-->A mutant, PCR from colleens wt FL into LIC vector using FH1 truncation primers, aa988-1529, 5polyPsAddgene 29718, pET His6 StrepII LICAmp39.2ng/uLI-->A mutant, same as PG5, but truncated FH1 domain with 5 PolyPs
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PG10Laura3LIM domains (from zyxin) into Sumo vector (addgene 29659)Addgene 29659, pET His6 Sumo TEV LIC cloning vector (1S)Kan30 ng/uL
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PG11Laura3LIM domains (from zyxin) into mCherry vector (addgene 29722)Addgene 29722, pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)Kan86 ng/uL
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PG12Laura3LIM domains (from zyxin) into GFP vector (addgene 29725)Addgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan75 ng/uL
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PG13GregHis-Strep-Zyxin Amp48 ng/uL
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PG14GregHis-GFP-Zyxin Addgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan59 ng/uL
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PG15GregHis-mCherry-Zyxin Addgene 29722, pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)Kan54 ng/uL
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PG16Lindsay CaseFL Vinculin-GFPnot sure620 ng/uL
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PG17Sharon CampbellHis-Strep-FL-VinculinTail (Vt) - chicken vinculin(not sure) Addgene 29718, pET His6 StrepII LICAmp26 ng/uLFL Vt = E879 - Q1066
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PG18LauraHis-Strep-delH1-VtAmp54 ng/uLVinculin tail (Vt) minus helix 1 (H1). This is the FL Vt (not delC5) (aa G916 - K1061)
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PG19LauraAddgene 29718, pET His6 StrepII LICAmp65 ng/uL
Vinculin tail (Vt) minus helix 1 (H1) and minus last 5 C-ter amino acids (delC5) (aa G916 - K1061)
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PG20LauraHis-GFP-E879-delC5-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan130 ng/uLVt w/ N-ter GFP at E879; minus last 5 C-ter amino acids (delC5)
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PG21LauraHis-GFP-H1-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan67 ng/uLHelix1 (H1) of Vt with an N-ter GFP
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PG22LauraHis-GFP-S914-delC5-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan66 ng/uLVt w/ N-ter GFP at S914; minus last 5 C-ter amino acids (delC5)
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PG23LauraHis-GFP-Q896-delC5-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan95 ng/uLVt w/ N-ter GFP at Q896; minus last 5 C-ter amino acids (delC5)
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PG24LauraHis-GFP-R903-delC5-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan64 ng/uLVt w/ N-ter GFP at R903; minus last 5 C-ter amino acids (delC5)
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PG25LauraHis-GFP-E892-delC5-VtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan66 ng/uLVt w/ N-ter GFP at E892; minus last 5 C-ter amino acids (delC5)
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PG26LauraHis-Strep-FL-MVtAddgene 29718, pET His6 StrepII LICAmp45 ng/uLFL metavinculin tail (MVt) in His-Strep vector (aa 858-1134)
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PG27LauraHis-Strep-FL-MVt-R975WAddgene 29718, pET His6 StrepII LICAmp47 ng/uLFL metavinculin tail (MVt) in His-Strep vector w/ R975W mutation
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PG28LauraHis-Strep-delC5-MVtAddgene 29718, pET His6 StrepII LICAmp41 ng/uLMVt minus last 5 C-ter amino acids (delC5) in His-Strep vector (aa 858-1129)
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PG29LauraHis-Strep-delN21-MVtAddgene 29718, pET His6 StrepII LICAmp48 ng/uLMVt minus first 21 N-ter amino acids (delN21) in His-Strep vector (aa 879-1134)
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PG30LauraHis-Strep-delC5-MVt-R975WAddgene 29718, pET His6 StrepII LICAmp46 ng/uL
MVt minus last 5 C-ter amino acids (delC5) in His-Strep vector (aa 858-1129) w/ R975W mutation
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PG31LauraHis-Strep-delN21-MVt-R975WAddgene 29718, pET His6 StrepII LICAmp89 ng/uL
MVt minus first 21 N-ter amino acids (delN21) in His-Strep vector (aa 879-1134) w/ R975W mutation
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PG32LauraHis-GFP-H1H1'-MVtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan96 ng/uLMVt aa 879-984 w/ Nter GFP
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PG33LauraHis-GFP-H1'-MVtAddgene 29725, pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)Kan131 ng/uLMVt aa 916-984 w/ Nter GFP
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PG34Bob (from Lindsay?)"vinc c-term"n/an/a120 ng/uLi don't know
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PG35Bob (Genecopeia)FL ABLIM1 in M98 vector pEZ M98 (C-ter eGFP)Amp/Neo55 ng/uL
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PG36Bob (Genecopeia)FL HIC5 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo75 ng/uL
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PG37Bob (Genecopeia)FL LASP1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo40 ng/uL
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PG38Bob (Genecopeia)FL LBD3 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo115 ng/uL
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PG39Bob (Genecopeia)FL LIMK1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo60 ng/uL
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PG40Bob (Genecopeia)FL LIMA1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo65 ng/uL
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PG41Bob (Genecopeia)FL LIMCH1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo42 ng/uL
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PG42Bob (Genecopeia)FL LIMD1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo37 ng/uL
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PG43Bob (Genecopeia)FL LMCD1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo98 ng/uL
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PG44Bob (Genecopeia)FL LMO7 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo62 ng/uL
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PG45Bob (Genecopeia)FL LPP in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo100 ng/uL
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PG46Bob (Genecopeia)FL Migfilin (FBLIM1) in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo125 ng/uL
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PG47Bob (Genecopeia)FL Paxillin in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo85 ng/uL
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PG48Bob (Genecopeia)FL PDLIM2 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo235 ng/uL
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PG49Bob (Genecopeia)FL PDLIM5 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo100 ng/uL
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PG50Bob (Genecopeia)FL PDLIM7 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo105 ng/uL
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PG51Bob (Genecopeia)FL Testin in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo155 ng/uL
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PG52Bob (Genecopeia)FL Trip6 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo140 ng/uL
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PG53Bob (Genecopeia)FL Zyxin in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo115 ng/uL
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PG54LauraLIM domain(s) of Lasp1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo785 ng/uL
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PG55LauraLIM domain(s) of LIMK1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo830 ng/uL
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PG56LauraLIM domain(s) of LIMCH1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo550 ng/uL
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PG57LauraLIM domain(s) of LIMD1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo963 ng/uL
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PG58LauraLIM domain(s) of LMO7 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo900 ng/uL
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PG59LauraLIM domain(s) of LPP in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo905 ng/uL
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PG60LauraLIM domain(s) of FBLIM1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo920 ng/uL
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PG61LauraLIM domain(s) of Paxillin in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo785 ng/uL
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PG62LauraLIM domain(s) of PDLIM2 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo904 ng/uL
64
PG63LauraLIM domain(s) of LMCD1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo850 ng/uL
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PG64LauraLIM domain(s) of TRIP6 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo750 ng/uL
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PG65LauraLIM domain(s) of Zyxin in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo850 ng/uL
67
PG6612/17/2003Pinarwt human profilin from Higgs lab via Tom PollardAmp44 ng/uL
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PG6711/22/2004PinarmDia1 FH1-FH2-C, aa549-1255 (2121bp), from Mike Rosen original FL mDia1 constructBamH1/Xho1pGEX-KTAmp47 ng/uL
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PG6811/5/2015Lauramini-prep DNA from PG66 (wt human profilin from Higgs lab via Tom Pollard)PMW plasmid Amp16 ng/uL
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PG6911/5/2015Lauramini-prep DNA from PG67 (mDia1 FH1-FH2-C, aa549-1255 (2121bp), from Mike Rosen original FL mDia1 construct)pGEX-KTAmp23 ng/uL
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PG7011/23/2015Pinarwt FMN2 FH2 PCR'd from PG4 (wt full length FMN2 from Colleen). aa 1139-1529. Used primers: LK2 and LK28Addgene 29718, pET His6 StrepII LICamp11.7ng/uL
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PG7111/23/2015PinarI1225A mutant FMN2 FH2 PCR'd from full length I-->A FMN2 from colleen. Primers LK2 and LK28Addgene 29718, pET His6 StrepII LICamp14.8ng/uL
73
PG721/13/2016LauraLIM domain(s) of LIMA1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo80 ng/uL
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PG734/8/2016Luke/Jordanmyo18a APEX vector from jordan beachAPEXn/a34 ng/uLuse it for PCRing out a workable APEX2 sequence
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PG744/8/2016LauraFL FHL2 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo5 ng/uL
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PG754/8/2016LauraFL PDLIM4 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo34 ng/uL
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PG764/8/2016LauraFL PDLIM1 in M98 vectorpEZ M98 (C-ter eGFP)Amp/Neo41 ng/uL
78
PG774/14/2016LauraFL-PDLIM1-apex in m98 vectorpEZ M98 (C-ter eGFP)Amp/Neo75 ng/uL
79
PG784/14/2016LauraFL-PDLIM4-apex in m98 vectorpEZ M98 (C-ter eGFP)Amp/Neo107 ng/uL
80
PG794/14/2016LauraFL-FHL2-apex in m98 vectorpEZ M98 (C-ter eGFP)Amp/Neo110 ng/uL
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PG804/19/2016LauraFL-zyxin-apex in m98 vectorpEZ M98 (C-ter eGFP)Amp/Neo73 ng/uL
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PG814/22/2016LauraZyxin(-LIM)_+FHL2 in m98 vectorpEZ M98 (C-ter eGFP)Amp/Neo325 ng/uL
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PG824/25/2016Pinaralpha-actinin 1 (human) from waterman lab; tdEos-N termKan2ug/uL
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PG834/25/2016LauraHIC5 LIM domain only (LDO) in m98pEZ M98 (C-ter eGFP)Amp/Neo90 ng/uL
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PG845/2/2016LauraZyxin (-LIM) in m98 vector (NOTE- rechecked sequencing and found this is not correct!! do not use this!)pEZ M98 (C-ter eGFP)Amp/Neo217 ng/uL
86
PG855/3/2016Pinaralpha-actinin 1 (human) N+ABD (from PG82) into LIC vector, aa 1-247Addgene 29718, pET His6 StrepII LICamp29.8 ng/uL
87
PG865/3/2016Pinaralpha-actinin 1 (human) ABD only (from PG82) into LIC vector, aa 31-247Addgene 29718, pET His6 StrepII LICamp24.4 ng/uL
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PG875/11/2016LauraAbLIM1-LDO in m98pEZ M98 (C-ter eGFP)amp250 ng/uL
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PG885/11/2016LauraLBD3-LDO in m98pEZ M98 (C-ter eGFP)amp254 ng/uL
90
PG895/11/2016LauraPDLIM7-LDO in m98pEZ M98 (C-ter eGFP)amp170 ng/uL
91
PG905/17/2016LauraZyxin-LDO_Trip6LIM1_ZyxLIM2_ZyxLIM3 in m98pEZ M98 (C-ter eGFP)amp29 ng/uL
92
PG915/17/2016LauraZyxin-LDO_ZyxLIM1_Trip6LIM2_ZyxLIM3 in m98pEZ M98 (C-ter eGFP)amp6 ng/uL
93
PG925/17/2016LauraZyxin-LDO_ZyxLIM1_ZyxLIM2_Trip6LIM3 in m98pEZ M98 (C-ter eGFP)amp15 ng/uL
94
PG936/9/2016LauraZyxin(-LIM) with SacII site in m98pEZ M98 (C-ter eGFP)amp206 ng/uL
95
PG946/9/2016LauraZyxin(-LIM) with BamHI site in m98pEZ M98 (C-ter eGFP)amp99 ng/uL
96
LA95LukePDLIM1 LIM domain (single) in vehicular plasmid with flanking BamHI and SacII sitesBlue Heron pUCminusMCS (no start codon)amp
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LA96LukePDLIM1 LIM domains (double) in vehicular plasmid with flanking BamHI and SacII sitesBlue Heron pUCminusMCS (no start codon)amp
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LA97LukePDLIM1 LIM domains (quad) in vehicular plasmid with flanking BamHI and SacII sitesBlue Heron pUCminusMCS (no start codon)amp
99
LA98LukePDLIM4 LIM domain (single) in vehicular plasmid with flanking BamHI and SacII sitesBlue Heron pUCminusMCS (no start codon)amp
100
LA99LukePDLIM4 LIM domains (double) in vehicular plasmid with flanking BamHI and SacII sitesBlue Heron pUCminusMCS (no start codon)amp