2012_12_17 test biological replicates for T7 expression
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

 
View only
 
 
Still loading...
ABCDEFGHIJKLMNOPQRST
1
2012_12_17: continue numbering from old spreadsheet:
2
https://docs.google.com/spreadsheet/ccc?key=0AlVxrZi130nMdGN0MC15VXdWallMbjBrVmdHU3htQnc#gid=10
3
4
Frozen stocks:DescriptionDateTube ColorVolumeSourceExperiments used for:
5
1strain 310/31/2012yellowsmall vol
6
2strain 510/31/2012yellowsmall vol
7
3strain 311/9/2012green~ 1mL
8
4strain 511/9/2012green~ 1mL
9
5strain 312/7/2012~ 1mL
10
6strain 512/7/2012~ 1mL
11
7control 311/28/2012~ 1mL
12
8control 511/28/2012~ 1mL
13
93inoculated 12/17plate 4 & culture 55
14
105inoculated 12/17plate 5 & culture 67
15
16
17
18
Plate:DescriptionSourceDate StreakedComments
19
1strain 3Transformed from 1st batch of stored comp cells (-80oC, green tubes)transformed 12/2
20
2strain 5Transformed from 1st batch of stored comp cells (-80oC, green tubes)transformed 12/2
21
3Control 3 & 5frozen stock #s 7 & 8
22
4strains 3 & 5colony 1 from tubes on desk from expression experiment that worked (?? was it the overnight or the induced??)12/16
23
24
6strains 3 & 5-80oC stocks 1 & 212/16
25
7strains 3 & 5-80oC stocks 3 & 412/16
26
8strains 3 & 5-80oC stocks 5 & 612/16
27
9strain 3culture tubes # 57 - 60 after ~ 3-4 hours post inoculation12/17/2012
28
10strain 5culture tubes # 61 - 64 after ~ 3-4 hours post inoculation12/17/2012
29
11strain 3 Fresh transform from competent cells prepped by Amanda 10/19.12/18/2012
30
12strain 5 Fresh transform from competent cells prepped by Amanda 10/19.12/18/2012
31
13strain 6NOTE: abandoning the old nomenclature from the first round when we named 3 & 5. Fresh transform from competent cells prepped by Amanda 10/19.12/18/2012
32
14strain 3restreak fresh colonies from plate 1. See if they grow better than cultures 55-6612/18/2012
33
15strain 512/18/2012
34
16just pJ52 in BL21(DE3)freezer strain #35 (Amp plate), stored 9/291/3/2013colony size is very uniform (big!)
35
17EV control for pJ52 in BL21(DE3)freezer strain #36 (Amp plate), stored 11/81/3/2013colonies are heterogeneously sized
36
18comp BL21(DE3) with plas j52 (old & new)OLD (10/19/2012) batch by Amanda & new competent cell batch, stored Friday 1/4/20131/6/2013Amp plate
37
19"strain 3" made from 1/4 comp cellsnew competent cell batch, stored Friday 1/4/2013. ADDED J23110 J23114 to make "strain 3"1/6/2013Amp Kan
38
20"strain 5" made from 1/4 comp cellsnew competent cell batch, stored Friday 1/4/2013. ADDED J23114 J23114 to make "strain 5"1/6/2013Amp Kan
39
21control 3 & control 5control 3 & 5 (janet's -80oC stocks 41 & 42)1/6/2013Amp Kan
40
22BL21(DE3) + j52 and 3K3 EV1/4 competent cells. Added plasmid j36 (pSB3K3 EV)1/10/2013 - re-did 1/11 because only 1 colonyAmp Kan
41
22bBL21(DE3) + j52 and 3K3 EV1/4 competent cells. Added plasmid j36 (pSB3K3 EV)1/11/2013Amp Kanheterogeneous colony sizes! Small compared to 22b colonies
42
23BL21(DE3) + j52 and 3K3 RFP1/4 competent cells. Added plasmid j22 (pGA3K3 RFP)1/10/2013Amp Kan
43
24BL21(DE3) + j52 and 3K3 J23114 FocA1/4 competent cells. Added Amanda's plasmid pSB3K3 J23114 (lower) FocA 1/10/2013Amp Kanhttp://partsregistry.org/Part:BBa_J23100
44
25BL21(DE3) + j52 and 3K3 J23100 FocA1/4 competent cells. Added Amanda's plasmid pSB3K3 J23100 (higher) FocA1/10/2013Amp Kan
45
26BL21(DE3) + j52 and 4C5 J23114 FDH J23114 FocA1/4 competent cells. Added Amanda's plasmid pSB3K3 J23100 (higher) FocA1/10/2013Amp Cm
46
26bBL21(DE3) + j52 and 4C5 J23114 FDH J23114 FocA1/4 competent cells. Added Amanda's plasmid pSB3K3 J23100 (higher) FocA1/11/2013 -no colonies on 1/10 plate
47
27restreak of overnights in 1/13 inoculation: 155-157
48
28restreak of overnights in 1/13 inoculation: 161-163
49
29BL21(DE3) Artic Express competent cell streakcommercial chemically competent cells1/22/2013
50
30BL21(DE3) PlySS competent cell streakcommercial chemically competent cells1/22/2013
51
31BL21(DE3) Artic Express + plasmid j52commercial chemically competent cells (transformed)1/22/2013
52
32BL21(DE3) PlySS + plasmid j52commercial chemically competent cells (transformed)1/22/2013
53
33DE3 +pJ52streakd from comp cells prepped 1/25
54
34DE3+pJ52 +3K3 J23110 FDH J23114 FocA (Strain 3)transformed from comp cells prepped 1/25
55
35DE3+pJ52 +3K3 J23114 FDH J23114 FocA (Strain 5)transformed from comp cells prepped 1/25
56
36DE3 +pJ52 +3K3 EVtransformed from comp cells prepped 1/25
57
37DE3 +pJ52 +3K3 FocAtransformed from comp cells prepped 1/25
58
38DE3 +pJ52 +3K3 RFPtransformed from comp cells prepped 1/25
59
39Arctic +pJ52streakd from comp cells prepped 1/25note: Cm not used to maintain the pLysS plasmid! pLysS is incompatable with pSB3K3 anyway.
60
40Arctic+pJ52 +3K3 J23110 FDH J23114 FocA (Strain 3)transformed from comp cells prepped 1/25
61
41Arctic+pJ52 +3K3 J23114 FDH J23114 FocA (Strain 5)transformed from comp cells prepped 1/25
62
42Arctic +pJ52 +3K3 EVtransformed from comp cells prepped 1/25
63
43Arctic +pJ52 +3K3 FocAtransformed from comp cells prepped 1/25
64
44Arctic +pJ52 +3K3 RFPtransformed from comp cells prepped 1/25
65
45pLysS +pJ52streakd from comp cells prepped 1/25
66
46pLysS+pJ52 +3K3 J23110 FDH J23114 FocA (Strain 3)transformed from comp cells prepped 1/25
67
47pLysS+pJ52 +3K3 J23114 FDH J23114 FocA (Strain 5)transformed from comp cells prepped 1/25
68
48pLysS +pJ52 +3K3 EVtransformed from comp cells prepped 1/25
69
49pLysS +pJ52 +3K3 FocAtransformed from comp cells prepped 1/25
70
50pLysS +pJ52 +3K3 RFPtransformed from comp cells prepped 1/25
71
Competent Cells
72
Date
73
10/19/2013BL21(DE3) + plas j52 made by Amandapink label
74
1/4/2013BL21(DE3) + plas j52 from Janet's -80oC strain 35 stock (9/29)green tube, green label
75
76
77
78
79
80
81
82
38# of tubes
83
84
85
86
87
88
11strain 3 Fresh transform from competent cells prepped by Amanda 10/19.
89
12strain 5 Fresh transform from competent cells prepped by Amanda 10/19.
90
13strain 6NOTE: abandoning the old nomenclature from the first round when we named 3 & 5. Fresh transform from competent cells prepped by Amanda 10/19.
91
14strain 3restreak fresh colonies from plate 1. See if they grow better than cultures 55-66
92
15strain 5
93
just pJ52 in BL21(DE3)
94
95
96
97
98
99
100
Loading...
 
 
 
Plates, Freezer Stocks, & Competent Cells
Cultures Inoculated
Cultures Collected
Protein Gels
Cultures
M9 Media Calculations
Copy of 12/11 repeat 12/6-12/10
Copy of Cultures Inoculated - OLD
1/26 culture code