20181016_qPCR_Cgigas_gDNA_primer_test
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Master Mix Calcs
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CiCfVol. (uL)# RxnsTotal Vol (uL)
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Template (BB15 gDNA or BB16 gDNA)0.5
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2x Sso Fast EVAgreen 21106.666
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Pf (Cg_18s_1644_F SRID 1168 or EF1_qPCR_5' SRID 309)100.20.56.63.3
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Pr (Cg_18s_1750_R SRID 1168 or EF1_qPCR_3' SRID 310)100.20.56.63.3
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H2O8.56.656.1
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Total20Add 19.5uL to each well
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