20181017_Cgigas_ploidy_cDNA_calcs
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sample_ID
Dnased_RNA(ng/uL)
vol_to_100ng(uL)
vol_dT_primers_1:10_dilution(uL)
primer_denature_vol(uL)
vol_h2o_to_15uL(uL)vol_5x_rxn_buffer(uL)
vol_10mM_dNTPs(uL)
vol_MMLV(uL)
vol_h2o_to_25uL(uL)vol_final(uL)
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Dnased_D01-C25.13.981157.0351.250.13.6525
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Dnased_D02-C20.34.931155.1551.250.13.6525
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Dnased_D09-C23.84.201156.6051.250.13.6525
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Dnased_D10-C21.94.571155.8751.250.13.6525
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Dnased_D11-C20.14.981155.0551.250.13.6525
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Dnased_D12-C27.33.661157.6751.250.13.6525
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Dnased_D19-C13.97.191150.6151.250.13.6525
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Dnased_D20-C25.23.971157.0651.250.13.6525
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Dnased_T01-C22.44.461156.0751.250.13.6525
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Dnased_T02-C21.44.671155.6551.250.13.6525
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Dnased_T09-C25.23.971157.0651.250.13.6525
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Dnased_T10-C19.95.031154.9551.250.13.6525
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Dnased_T11-C20.64.851155.2951.250.13.6525
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Dnased_T12-C18.25.491154.0151.250.13.6525
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Dnased_T19-C25.93.861157.2851.250.13.6525
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Dnased_T20-C26.53.771157.4551.250.13.6525
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MMLV_master_mix
vol_single rxn
number of reactions
vol_total(uL)
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5x_rxn_buffer51890
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10mM_dNTPs1.251822.5
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H2O3.651865.7
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MMLV0.1181.8
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Add 10uL to each sample.
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