20150616_Jake_Oly_cDNA_Calcs
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ABCDEFGHIJKLMNOPQRST
1
Plate PositionSample ID[DNased RNA] (ng/ul) uL to 250ng RNAuL dT primers (0.5ug/ug RNA) of a 1:10 dilutionH2O to 74.75uL5x rxn buffer (uL)10mM dNTPs (uL)MMLV (uL)Master mixSingle Reaction (uL)# Reactions
Total (uL)
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A07DNased 42215 HC181.163.081.2570.422050.255x buffer20531060
3
B07DNased 42215 HC279.933.131.2570.372050.25dNTPs553265
4
C07DNased 42215 HC379.083.161.2570.342050.25MMLV0.255313.25
5
D07DNased 42215 HC4793.161.2570.342050.25
6
E07DNased 42215 HC582.943.011.2570.492050.25
7
F07DNased 42215 HC680.973.091.2570.412050.25
8
G07DNased 42215 HC784.082.971.2570.532050.25
9
H07DNased 42215 HC879.943.131.2570.372050.25
10
A08DNased 42215 NC179.23.161.2570.342050.25
11
B08DNased 42215 NC291.372.741.2570.762050.25
12
C08DNased 42215 NC376.323.281.2570.222050.25
13
D08DNased 42215 NC483.313.001.2570.502050.25
14
E08DNased 42215 NC583.752.991.2570.512050.25
15
F08DNased 42215 NC685.912.911.2570.592050.25
16
G08DNased 42215 NC782.13.051.2570.452050.25
17
H08DNased 42215 NC874.523.351.2570.152050.25
18
A09DNased 42215 SC178.113.201.2570.302050.25
19
B09DNased 42215 SC284.152.971.2570.532050.25
20
C09DNased 42215 SC379.463.151.2570.352050.25
21
D09DNased 42215 SC484.982.941.2570.562050.25
22
E09DNased 42215 SC581.993.051.2570.452050.25
23
F09DNased 42215 SC670.293.561.2569.942050.25
24
G09DNased 42215 SC777.083.241.2570.262050.25
25
H09DNased 42215 SC884.222.971.2570.532050.25
26
A10DNased 42215 HT1 124.2910.291.2563.212050.25
27
B10DNased 42215 HT1 225.139.951.2563.552050.25
28
C10DNased 42215 HT1 324.210.331.2563.172050.25
29
D10DNased 42215 HT1 423.4510.661.2562.842050.25
30
E10DNased 42215 HT1 524.4210.241.2563.262050.25
31
F10DNased 42215 HT1 623.8510.481.2563.022050.25
32
G10DNased 42215 HT1 731.597.911.2565.592050.25
33
H10DNased 42215 HT1 8119.542.091.2571.412050.25
34
A11DNased 42215 NT1 135.187.111.2566.392050.25
35
B11DNased 42215 NT1 225.279.891.2563.612050.25
36
C11DNased 42215 NT1 323.0210.861.2562.642050.25
37
D11DNased 42215 NT1 424.2110.331.2563.172050.25
38
E11
DNased 42215 NT1 523.4110.681.2562.822050.25
39
F11DNased 42215 NT1 624.6310.151.2563.352050.25
40
G11DNased 42215 NT1 718.1513.771.2559.732050.25
41
H11DNased 42215 NT1 87.1335.061.2538.442050.25
42
A12DNased 42215 ST1 122.9110.911.2562.592050.25
43
B12DNased 42215 ST1 222.5711.081.2562.422050.25
44
C12DNased 42215 ST1 324.1810.341.2563.162050.25
45
D12DNased 42215 ST1 428.658.731.2564.772050.25
46
E12DNased 42215 ST1 526.289.511.2563.992050.25
47
F12DNased 42215 ST1 619.9812.511.2560.992050.25
48
G12DNased 42215 ST1 724.410.251.2563.252050.25
49
H12DNased 42215 ST1 823.2610.751.2562.752050.25
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Sheet1