ABCDEFGHIJKLMNOPQRSTUVWXYZ
1
2
Pre-plate 6/28mRuby (+ctrl, 1x)mRuby (+ctrl, 2x)Notes
3
Transfect 6/291.5 lipo DNA1.5 lipo DNA
mRuby3 is in Freezer A. Use red cap tube mRuby3-1B (has a concentration of 790.15ng/uL)
4
View 7/2RPMIRPMI
SARS-CoV-S-RBD-sfGFP_pcDNA3-1 is in Freezer A. It is labeled with a green cap SARS-RBD-GFP-1 and has a concentration of 1187.43ng/uL
5
eGFP (+ctrl, 1x)eGFP (+ctrl, 2x)RBD-eGFP XmaI (1x)RBD-eGFP XmaI (2x)
eGFP stock solution is 6112ng/uL (DILUTE .25 µL BY 1/2 BEFORE STARTING)
6
1.5 lipo DNA1.5 lipo DNA1.5 lipo RNA1.5 lipo RNA
in magnetic project box in freezer c
7
RPMIRPMIRPMIRPMI
8
RBD-eGFP (1x)RBD-eGFP (2x)RBD-eGFP XhoI (1x)RBD-eGFP XhoI (2x)
9
1.5 lipo DNA1.5 lipo DNA1.5 lipo RNA1.5 lipo RNA
10
RPMIRPMIRPMIRPMI
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100