IGI Shared CRISPRi/a cell lines
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IDParental cell lineParental cell line sourceCRISPRi/CRISPRaClonal population (Y/N)Clone nameConstruct nameIGI Plasmid codeAddgene Plasmid codeDepositing LabCells made byGrowth mediaOther notesName for Distribution
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1Jurkat E6.1Berkeley TC facilityCRISPRiYNH7pHR-EF1a-dCas9-BFP-KRABIGI-P0152Not yet depositedJacob CornBenjamin GowenRPMI, 10% FCS, Pen-Strep, Sodium PyruvateCas9 is HA-taggedJurkat CRISPRi
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2Jurkat E6.1Berkeley TC facilityCRISPRaYRF20Lenti dCas9-VP64-2A-GFPIGI-P0216Addgene 61422Jacob CornBenjamin GowenRPMI, 10% FCS, Pen-Strep, Sodium PyruvateJurkat CRISPRa (VP64)
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3HuT78Art Weiss, UCSFCRISPRaYAMB.6Lenti dCas9-VP64-2A-GFPIGI-P0216Addgene 61422Jacob CornMandy BoontanrartRPMI, 10% FCS, Pen-Strep, Sodium PyruvateHuT78 CRISPRa (VP64)
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4HCT116Berkeley TC facilityCRISPRiYHCT116-C6pHR-EF1a-dCas9-BFP-KRAB-NLSIGI-P0220Not yet depositedJacob CornJin Rui LiangCas9 is HA-taggedHCT116 CRISPRi
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5LK2AZ (?)CRISPRiYc16pSFFV-HA-dCas9KRAB-BFPIGI-P0021Addgene 46911Jacob CornPeiChun LinRPMI, 10% FCS, Pen-Strep, Sodium PyruvateCas9 is HA-taggedLK2 CRISPRi
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63T3-L1Berkeley TC facilityCRISPRiYClone 23pHR-EF1a-dCas9-BFP-KRAB-NLSIGI-P0220Not yet depositedJacob CornHong MaDMEM Hi-Glucose with Glutamine, 10% FBS, Pen-StrepCas9 is HA-tagged. Cells must be kept below 60% confluent and be passaged every 3 days.3T3-L1 CRISPRi
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7K562Berkeley TC facilityCRISPRiYK1epHR-hEF1a-dCas9-mcherry-KRAB-mPGK-HygpCR1063Not yet depositedJacob CornChris RichardsonRPMI, 10% FCS, Pen-Strep, Sodium PyruvateK562 CRISPRi
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