$458/96T $320/48T

500 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

100 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6 . After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>100.00</td><td>2.999</td><td class='1'>2.939</td></tr><tr><td>50.00</td><td>2.373</td><td class='2'>2.313</td></tr><tr><td>25.00</td><td>1.683</td><td class='3'>1.623</td></tr><tr><td>12.50</td><td>1.117</td><td class='4'>1.057</td></tr><tr><td>6.25</td><td>0.708</td><td class='5'>0.648</td></tr><tr><td>3.13</td><td>0.344</td><td class='6'>0.284</td></tr><tr><td>1.57</td><td>0.175</td><td class='7'>0.005</td></tr><tr><td>0.00</td><td>0.060</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant IL6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL6 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>EDTA plasma(n=5)</td><td>95-110%</td><td>100</td></tr><tr><td>Heparin plasma(n=5)</td><td>93-102%</td><td>98</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-98%</th><th>98-110%</th><th>99-116%</th><th>95-110%</th></tr><tr><td>EDTA plasma(n=5)</td><td>87-102%</td><td>85-95%</td><td>85-98%</td><td>82-95%</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-102%</td><td>95-106%</td><td>95-108%</td><td>96-115%</td></tr></tbody></table></div></div>

$458/96T $320/48T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA . After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>3.106</td><td class='1'>3.055</td></tr><tr><td>5.00</td><td>2.024</td><td class='2'>1.973</td></tr><tr><td>2.50</td><td>1.179</td><td class='3'>1.129</td></tr><tr><td>1.25</td><td>0.691</td><td class='4'>0.641</td></tr><tr><td>0.63</td><td>0.372</td><td class='5'>0.322</td></tr><tr><td>0.32</td><td>0.217</td><td class='6'>0.167</td></tr><tr><td>0.16</td><td>0.144</td><td class='7'>0.094</td></tr><tr><td>0.00</td><td>0.050</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant CEA and the recovery rates were calculated by comparing the measured value to the expected amount of CEA in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>92-110%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>88-105%</td><td>95</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-102%</td><td>98</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>93-105%</th><th>85-102%</th><th>91-110%</th><th>91-108%</th></tr><tr><td>EDTA plasma(n=5)</td><td>90-104%</td><td>90-106%</td><td>88-108%</td><td>92-109%</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-103%</td><td>94-108%</td><td>95-105%</td><td>88-107%</td></tr></tbody></table></div></div>

$458/96T $320/48T

500 pg/ml

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCT, and the Human PCT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PCT . After TMB substrate solution is added, only those wells that contain Human PCT and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCT in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>500.00</td><td>2.558</td><td class='1'>2.478</td></tr><tr><td>250.00</td><td>1.476</td><td class='2'>1.397</td></tr><tr><td>125.00</td><td>0.853</td><td class='3'>0.773</td></tr><tr><td>62.50</td><td>0.558</td><td class='4'>0.478</td></tr><tr><td>31.25</td><td>0.324</td><td class='5'>0.245</td></tr><tr><td>15.63</td><td>0.218</td><td class='6'>0.138</td></tr><tr><td>7.82</td><td>0.174</td><td class='7'>0.094</td></tr><tr><td>0.00</td><td>0.080</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PCT and the recovery rates were calculated by comparing the measured value to the expected amount of PCT in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-101%</td><td>93</td></tr><tr><td>EDTA plasma(n=5)</td><td>94-103%</td><td>99</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-105%</td><td>98</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>89-101%</th><th>85-98%</th><th>88-99%</th><th>85-101%</th></tr><tr><td>EDTA plasma(n=5)</td><td>95-106%</td><td>96-108%</td><td>90-105%</td><td>86-102%</td></tr><tr><td>Heparin plasma(n=5)</td><td>91-104%</td><td>88-102%</td><td>92-100%</td><td>89-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.028 ng/ml

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AMH, and the Human AMH standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human AMH . After TMB substrate solution is added, only those wells that contain Human AMH and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.503</td><td class='1'>2.403</td></tr><tr><td>10.00</td><td>1.581</td><td class='2'>1.481</td></tr><tr><td>5.00</td><td>0.890</td><td class='3'>0.790</td></tr><tr><td>2.50</td><td>0.523</td><td class='4'>0.423</td></tr><tr><td>1.25</td><td>0.343</td><td class='5'>0.243</td></tr><tr><td>0.63</td><td>0.220</td><td class='6'>0.120</td></tr><tr><td>0.32</td><td>0.152</td><td class='7'>0.053</td></tr><tr><td>0.00</td><td>0.100</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant AMH and the recovery rates were calculated by comparing the measured value to the expected amount of AMH in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-115%</td><td>103</td></tr><tr><td>EDTA plasma(n=5)</td><td>85-106%</td><td>98</td></tr><tr><td>Heparin plasma(n=5)</td><td>92-108%</td><td>101</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>80-112%</th><th>85-105%</th><th>91-110%</th><th>93-105%</th></tr><tr><td>EDTA plasma(n=5)</td><td>93-113%</td><td>86-106%</td><td>90-110%</td><td>97-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>96-107%</td><td>90-103%</td><td>88-110%</td><td>86-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

5000 pg/ml

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C . After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>5000.00</td><td>2.781</td><td class='1'>2.716</td></tr><tr><td>2500.00</td><td>1.701</td><td class='2'>1.637</td></tr><tr><td>1250.00</td><td>0.906</td><td class='3'>0.842</td></tr><tr><td>625.00</td><td>0.508</td><td class='4'>0.443</td></tr><tr><td>312.50</td><td>0.319</td><td class='5'>0.254</td></tr><tr><td>156.25</td><td>0.177</td><td class='6'>0.113</td></tr><tr><td>78.13</td><td>0.120</td><td class='7'>0.056</td></tr><tr><td>0.00</td><td>0.064</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-99%</td><td>94</td></tr><tr><td>EDTA plasma(n=5)</td><td>102-110%</td><td>104</td></tr><tr><td>Heparin plasma(n=5)</td><td>98-104%</td><td>101</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>93-106%</th><th>88-102%</th><th>85-101%</th><th>96-107%</th></tr><tr><td>EDTA plasma(n=5)</td><td>90-103%</td><td>94-108%</td><td>90-106%</td><td>98-105%</td></tr><tr><td>Heparin plasma(n=5)</td><td>91-100%</td><td>98-107%</td><td>92-108%</td><td>93-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

800pg/ml

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GH, and the Human GH standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GH . After TMB substrate solution is added, only those wells that contain Human GH and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GH in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>800.00</td><td>2.443</td><td class='1'>2.393</td></tr><tr><td>400.00</td><td>1.779</td><td class='2'>1.729</td></tr><tr><td>200.00</td><td>1.075</td><td class='3'>1.024</td></tr><tr><td>100.00</td><td>0.561</td><td class='4'>0.511</td></tr><tr><td>50.00</td><td>0.322</td><td class='5'>0.271</td></tr><tr><td>25.00</td><td>0.195</td><td class='6'>0.145</td></tr><tr><td>12.50</td><td>0.121</td><td class='7'>0.070</td></tr><tr><td>0.00</td><td>0.050</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant GH and the recovery rates were calculated by comparing the measured value to the expected amount of GH in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>96-109%</td><td>104</td></tr><tr><td>EDTA plasma(n=5)</td><td>98-108%</td><td>101</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-102%</td><td>96</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>89-101%</th><th>99-108%</th><th>93-101%</th><th>93-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>91-102%</td><td>94-101%</td><td>93-104%</td><td>96-109%</td></tr><tr><td>Heparin plasma(n=5)</td><td>83-97%</td><td>90-107%</td><td>88-96%</td><td>94-103%</td></tr></tbody></table></div></div>

$458/96T $320/48T

2000 pg/ml

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP . After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>2000.00</td><td>2.266</td><td class='1'>2.170</td></tr><tr><td>1000.00</td><td>1.633</td><td class='2'>1.537</td></tr><tr><td>500.00</td><td>1.088</td><td class='3'>0.992</td></tr><tr><td>250.00</td><td>0.826</td><td class='4'>0.730</td></tr><tr><td>125.00</td><td>0.539</td><td class='5'>0.443</td></tr><tr><td>62.50</td><td>0.313</td><td class='6'>0.217</td></tr><tr><td>31.25</td><td>0.235</td><td class='7'>0.139</td></tr><tr><td>0.00</td><td>0.096</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant BNP and the recovery rates were calculated by comparing the measured value to the expected amount of BNP in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-102%</td><td>95</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-93%</td><td>86</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-97%</td><td>90</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>87-98%</th><th>85-92%</th><th>79-96%</th><th>89-100%</th></tr><tr><td>EDTA plasma(n=5)</td><td>86-102%</td><td>87-96%</td><td>89-98%</td><td>83-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>98-105%</td><td>92-101%</td><td>85-105%</td><td>87-98%</td></tr></tbody></table></div></div>

$458/96T $320/48T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sST2, and the Human sST2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human sST2 . After TMB substrate solution is added, only those wells that contain Human sST2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sST2 in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>30.00</td><td>2.035</td><td class='1'>1.939</td></tr><tr><td>15.00</td><td>1.502</td><td class='2'>1.406</td></tr><tr><td>7.50</td><td>1.115</td><td class='3'>1.019</td></tr><tr><td>3.75</td><td>0.892</td><td class='4'>0.796</td></tr><tr><td>1.88</td><td>0.563</td><td class='5'>0.467</td></tr><tr><td>0.94</td><td>0.239</td><td class='6'>0.143</td></tr><tr><td>0.47</td><td>0.213</td><td class='7'>0.117</td></tr><tr><td>0.00</td><td>0.096</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant sST2 and the recovery rates were calculated by comparing the measured value to the expected amount of sST2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-108%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-97%</td><td>89</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sST2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>90-99%</th><th>83-97%</th><th>81-103%</th><th>89-101%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-99%</td><td>81-93%</td><td>89-97%</td><td>85-94%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-96%</td><td>82-95%</td><td>93-102%</td><td>87-96%</td></tr></tbody></table></div></div>

$458/96T $320/48T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC . After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>15.00</td><td>2.126</td><td class='1'>2.025</td></tr><tr><td>7.50</td><td>1.572</td><td class='2'>1.471</td></tr><tr><td>3.75</td><td>1.116</td><td class='3'>1.015</td></tr><tr><td>1.88</td><td>0.861</td><td class='4'>0.760</td></tr><tr><td>0.94</td><td>0.538</td><td class='5'>0.437</td></tr><tr><td>0.47</td><td>0.329</td><td class='6'>0.228</td></tr><tr><td>0.24</td><td>0.192</td><td class='7'>0.091</td></tr><tr><td>0.00</td><td>0.101</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant OC and the recovery rates were calculated by comparing the measured value to the expected amount of OC in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>EDTA plasma(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>Heparin plasma(n=5)</td><td>81-95%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>86-97%</th><th>88-95%</th><th>92-101%</th><th>87-98%</th></tr><tr><td>EDTA plasma(n=5)</td><td>78-92%</td><td>82-96%</td><td>86-99%</td><td>93-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-96%</td><td>86-98%</td><td>92-103%</td><td>87-93%</td></tr></tbody></table></div></div>

$458/96T $320/48T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGII, and the Human PGII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PGII . After TMB substrate solution is added, only those wells that contain Human PGII and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGII in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.058</td><td class='1'>1.965</td></tr><tr><td>5.00</td><td>1.628</td><td class='2'>1.535</td></tr><tr><td>2.50</td><td>1.109</td><td class='3'>1.016</td></tr><tr><td>1.25</td><td>0.821</td><td class='4'>0.728</td></tr><tr><td>0.63</td><td>0.474</td><td class='5'>0.381</td></tr><tr><td>0.32</td><td>0.361</td><td class='6'>0.268</td></tr><tr><td>0.16</td><td>0.228</td><td class='7'>0.135</td></tr><tr><td>0.00</td><td>0.093</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PGII and the recovery rates were calculated by comparing the measured value to the expected amount of PGII in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>EDTA plasma(n=5)</td><td>88-103%</td><td>95</td></tr><tr><td>Heparin plasma(n=5)</td><td>92-106%</td><td>99</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>87-102%</th><th>82-91%</th><th>86-93%</th><th>81-94%</th></tr><tr><td>EDTA plasma(n=5)</td><td>91-98%</td><td>83-101%</td><td>87-98%</td><td>95-102%</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-97%</td><td>78-96%</td><td>80-99%</td><td>89-97%</td></tr></tbody></table></div></div>

$458/96T $320/48T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA . After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.268</td><td class='1'>2.195</td></tr><tr><td>10.00</td><td>1.502</td><td class='2'>1.429</td></tr><tr><td>5.00</td><td>1.073</td><td class='3'>1.000</td></tr><tr><td>2.50</td><td>0.892</td><td class='4'>0.819</td></tr><tr><td>1.25</td><td>0.518</td><td class='5'>0.445</td></tr><tr><td>0.63</td><td>0.312</td><td class='6'>0.239</td></tr><tr><td>0.32</td><td>0.186</td><td class='7'>0.113</td></tr><tr><td>0.00</td><td>0.073</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PSA and the recovery rates were calculated by comparing the measured value to the expected amount of PSA in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>EDTA plasma(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>Heparin plasma(n=5)</td><td>81-95%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PSA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>88-101%</th><th>85-93%</th><th>89-97%</th><th>85-94%</th></tr><tr><td>EDTA plasma(n=5)</td><td>89-99%</td><td>88-104%</td><td>84-98%</td><td>95-105%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-97%</td><td>83-96%</td><td>91-103%</td><td>96-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.068 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC39A13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC39A13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC39A13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC39A13 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.268</td><td class='1'>2.186</td></tr><tr><td>5.00</td><td>1.527</td><td class='2'>1.445</td></tr><tr><td>2.50</td><td>1.106</td><td class='3'>1.024</td></tr><tr><td>1.25</td><td>0.928</td><td class='4'>0.846</td></tr><tr><td>0.63</td><td>0.519</td><td class='5'>0.437</td></tr><tr><td>0.32</td><td>0.357</td><td class='6'>0.275</td></tr><tr><td>0.16</td><td>0.221</td><td class='7'>0.139</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant SLC39A13 and the recovery rates were calculated by comparing the measured value to the expected amount of SLC39A13 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>82-98%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>88-99%</td><td>93</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-98%</td><td>92</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SLC39A13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>79-92%</th><th>89-102%</th><th>87-98%</th><th>80-92%</th></tr><tr><td>EDTA plasma(n=5)</td><td>82-95%</td><td>88-102%</td><td>84-97%</td><td>89-107%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-105%</td><td>82-95%</td><td>81-97%</td><td>93-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

11.2 pg/mL

3000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OLFM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OLFM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OLFM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OLFM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>3000.00</td><td>2.035</td><td class='1'>1.946</td></tr><tr><td>1500.00</td><td>1.566</td><td class='2'>1.477</td></tr><tr><td>750.00</td><td>1.052</td><td class='3'>0.963</td></tr><tr><td>375.00</td><td>0.866</td><td class='4'>0.777</td></tr><tr><td>187.50</td><td>0.625</td><td class='5'>0.536</td></tr><tr><td>93.75</td><td>0.315</td><td class='6'>0.226</td></tr><tr><td>46.88</td><td>0.168</td><td class='7'>0.079</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant OLFM1 and the recovery rates were calculated by comparing the measured value to the expected amount of OLFM1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>83-97%</td><td>90</td></tr><tr><td>EDTA plasma(n=5)</td><td>89-103%</td><td>96</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-99%</td><td>92</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OLFM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>91-102%</th><th>86-102%</th><th>87-99%</th><th>99-105%</th></tr><tr><td>EDTA plasma(n=5)</td><td>79-93%</td><td>89-97%</td><td>85-94%</td><td>87-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-94%</td><td>85-95%</td><td>89-99%</td><td>85-95%</td></tr></tbody></table></div></div>

$458/96T $320/48T

51.7 pg/mL

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NEFL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NEFL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NEFL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NEFL in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>1000.00</td><td>2.126</td><td class='1'>2.037</td></tr><tr><td>500.00</td><td>1.674</td><td class='2'>1.585</td></tr><tr><td>250.00</td><td>1.194</td><td class='3'>1.105</td></tr><tr><td>125.00</td><td>0.787</td><td class='4'>0.698</td></tr><tr><td>62.50</td><td>0.555</td><td class='5'>0.466</td></tr><tr><td>31.25</td><td>0.388</td><td class='6'>0.299</td></tr><tr><td>15.63</td><td>0.207</td><td class='7'>0.118</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant NEFL and the recovery rates were calculated by comparing the measured value to the expected amount of NEFL in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>78-92%</td><td>85</td></tr><tr><td>EDTA plasma(n=5)</td><td>77-95%</td><td>86</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-99%</td><td>93</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-94%</th><th>87-101%</th><th>86-97%</th><th>88-95%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>93-101%</td><td>97-103%</td><td>91-99%</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-98%</td><td>85-92%</td><td>83-96%</td><td>93-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.049 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.035</td><td class='1'>1.942</td></tr><tr><td>5.00</td><td>1.523</td><td class='2'>1.430</td></tr><tr><td>2.50</td><td>1.154</td><td class='3'>1.061</td></tr><tr><td>1.25</td><td>0.828</td><td class='4'>0.735</td></tr><tr><td>0.63</td><td>0.411</td><td class='5'>0.318</td></tr><tr><td>0.32</td><td>0.388</td><td class='6'>0.295</td></tr><tr><td>0.16</td><td>0.208</td><td class='7'>0.115</td></tr><tr><td>0.00</td><td>0.093</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PIEZO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIEZO-1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-102%</td><td>95</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-92%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-97%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIEZO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>79-96%</th><th>85-99%</th><th>79-93%</th><th>89-97%</th></tr><tr><td>EDTA plasma(n=5)</td><td>95-102%</td><td>98-106%</td><td>87-98%</td><td>91-102%</td></tr><tr><td>Heparin plasma(n=5)</td><td>89-99%</td><td>88-104%</td><td>84-98%</td><td>95-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.048 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SEMA6B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SEMA6B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SEMA6B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SEMA6B in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.179</td><td class='1'>2.095</td></tr><tr><td>5.00</td><td>1.636</td><td class='2'>1.552</td></tr><tr><td>2.50</td><td>1.175</td><td class='3'>1.091</td></tr><tr><td>1.25</td><td>0.895</td><td class='4'>0.811</td></tr><tr><td>0.63</td><td>0.547</td><td class='5'>0.463</td></tr><tr><td>0.32</td><td>0.388</td><td class='6'>0.304</td></tr><tr><td>0.16</td><td>0.256</td><td class='7'>0.172</td></tr><tr><td>0.00</td><td>0.084</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant SEMA6B and the recovery rates were calculated by comparing the measured value to the expected amount of SEMA6B in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>82-95%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-96%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-107%</td><td>101</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEMA6B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>91-99%</th><th>83-89%</th><th>88-104%</th><th>97-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-99%</td><td>82-96%</td><td>92-101%</td><td>82-90%</td></tr><tr><td>Heparin plasma(n=5)</td><td>79-92%</td><td>80-93%</td><td>81-98%</td><td>89-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.11 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RCN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RCN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RCN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RCN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.532</td><td class='1'>2.435</td></tr><tr><td>10.00</td><td>1.771</td><td class='2'>1.674</td></tr><tr><td>5.00</td><td>1.142</td><td class='3'>1.045</td></tr><tr><td>2.50</td><td>0.836</td><td class='4'>0.739</td></tr><tr><td>1.25</td><td>0.567</td><td class='5'>0.470</td></tr><tr><td>0.63</td><td>0.361</td><td class='6'>0.264</td></tr><tr><td>0.32</td><td>0.179</td><td class='7'>0.082</td></tr><tr><td>0.00</td><td>0.097</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant RCN3 and the recovery rates were calculated by comparing the measured value to the expected amount of RCN3 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>86-99%</td><td>92</td></tr><tr><td>EDTA plasma(n=5)</td><td>89-103%</td><td>96</td></tr><tr><td>Heparin plasma(n=5)</td><td>78-96%</td><td>87</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RCN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>79-93%</th><th>89-97%</th><th>85-94%</th><th>87-101%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-94%</td><td>78-96%</td><td>79-93%</td><td>82-90%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-103%</td><td>89-101%</td><td>87-98%</td><td>85-92%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.21 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGFR in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>50.00</td><td>2.252</td><td class='1'>2.175</td></tr><tr><td>25.00</td><td>1.612</td><td class='2'>1.535</td></tr><tr><td>12.50</td><td>1.182</td><td class='3'>1.105</td></tr><tr><td>6.25</td><td>0.876</td><td class='4'>0.799</td></tr><tr><td>3.13</td><td>0.539</td><td class='5'>0.462</td></tr><tr><td>1.57</td><td>0.313</td><td class='6'>0.236</td></tr><tr><td>0.79</td><td>0.198</td><td class='7'>0.121</td></tr><tr><td>0.00</td><td>0.077</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PTGFR and the recovery rates were calculated by comparing the measured value to the expected amount of PTGFR in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>78-92%</td><td>85</td></tr><tr><td>EDTA plasma(n=5)</td><td>92-107%</td><td>99</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-99%</td><td>93</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGFR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-96%</th><th>95-104%</th><th>93-102%</th><th>79-94%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-101%</td><td>87-103%</td><td>84-92%</td><td>78-90%</td></tr><tr><td>Heparin plasma(n=5)</td><td>91-98%</td><td>83-101%</td><td>87-98%</td><td>95-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAV3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAV3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAV3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAV3 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.075</td><td class='1'>1.984</td></tr><tr><td>5.00</td><td>1.527</td><td class='2'>1.436</td></tr><tr><td>2.50</td><td>1.118</td><td class='3'>1.027</td></tr><tr><td>1.25</td><td>0.882</td><td class='4'>0.791</td></tr><tr><td>0.63</td><td>0.576</td><td class='5'>0.485</td></tr><tr><td>0.32</td><td>0.305</td><td class='6'>0.214</td></tr><tr><td>0.16</td><td>0.186</td><td class='7'>0.095</td></tr><tr><td>0.00</td><td>0.091</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant CAV3 and the recovery rates were calculated by comparing the measured value to the expected amount of CAV3 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>91</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-103%</td><td>96</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CAV3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-90%</th><th>83-96%</th><th>95-102%</th><th>88-95%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-102%</td><td>87-96%</td><td>82-90%</td><td>92-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>79-92%</td><td>85-96%</td><td>80-98%</td><td>95-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

13.3 pg/mL

3000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>3000.00</td><td>2.204</td><td class='1'>2.117</td></tr><tr><td>1500.00</td><td>1.593</td><td class='2'>1.506</td></tr><tr><td>750.00</td><td>1.175</td><td class='3'>1.088</td></tr><tr><td>375.00</td><td>0.895</td><td class='4'>0.808</td></tr><tr><td>187.50</td><td>0.521</td><td class='5'>0.434</td></tr><tr><td>93.75</td><td>0.366</td><td class='6'>0.279</td></tr><tr><td>46.88</td><td>0.302</td><td class='7'>0.215</td></tr><tr><td>0.00</td><td>0.087</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant BCAT2 and the recovery rates were calculated by comparing the measured value to the expected amount of BCAT2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>82-95%</td><td>88</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-94%</td><td>87</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCAT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-96%</th><th>87-101%</th><th>89-102%</th><th>85-98%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-94%</td><td>79-96%</td><td>92-101%</td><td>83-90%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-102%</td><td>93-101%</td><td>97-105%</td><td>95-103%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.051 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>1.996</td><td class='1'>1.910</td></tr><tr><td>5.00</td><td>1.502</td><td class='2'>1.416</td></tr><tr><td>2.50</td><td>1.157</td><td class='3'>1.071</td></tr><tr><td>1.25</td><td>0.896</td><td class='4'>0.810</td></tr><tr><td>0.63</td><td>0.527</td><td class='5'>0.441</td></tr><tr><td>0.32</td><td>0.312</td><td class='6'>0.226</td></tr><tr><td>0.16</td><td>0.190</td><td class='7'>0.104</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant BCAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of BCAT1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>81-95%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-92%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-106%</td><td>97</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>87-96%</th><th>91-101%</th><th>87-98%</th><th>89-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-94%</td><td>87-96%</td><td>79-95%</td><td>95-102%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-92%</td><td>79-96%</td><td>87-103%</td><td>90-99%</td></tr></tbody></table></div></div>

$458/96T $320/48T

31.2 pg/mL

6000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>6000.00</td><td>2.058</td><td class='1'>1.961</td></tr><tr><td>3000.00</td><td>1.412</td><td class='2'>1.315</td></tr><tr><td>1500.00</td><td>1.128</td><td class='3'>1.031</td></tr><tr><td>750.00</td><td>0.864</td><td class='4'>0.767</td></tr><tr><td>375.00</td><td>0.473</td><td class='5'>0.376</td></tr><tr><td>187.50</td><td>0.324</td><td class='6'>0.227</td></tr><tr><td>93.75</td><td>0.179</td><td class='7'>0.082</td></tr><tr><td>0.00</td><td>0.097</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant EPN1 and the recovery rates were calculated by comparing the measured value to the expected amount of EPN1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>96-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-97%</td><td>91</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>95-102%</th><th>87-96%</th><th>78-92%</th><th>85-97%</th></tr><tr><td>EDTA plasma(n=5)</td><td>82-93%</td><td>96-105%</td><td>87-98%</td><td>85-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-104%</td><td>82-93%</td><td>81-96%</td><td>83-94%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.048 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LRP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LRP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LRP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.043</td><td class='1'>1.961</td></tr><tr><td>5.00</td><td>1.703</td><td class='2'>1.621</td></tr><tr><td>2.50</td><td>1.143</td><td class='3'>1.061</td></tr><tr><td>1.25</td><td>0.796</td><td class='4'>0.714</td></tr><tr><td>0.63</td><td>0.514</td><td class='5'>0.432</td></tr><tr><td>0.32</td><td>0.315</td><td class='6'>0.233</td></tr><tr><td>0.16</td><td>0.129</td><td class='7'>0.047</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant LRP3 and the recovery rates were calculated by comparing the measured value to the expected amount of LRP3 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>85-97%</td><td>91</td></tr><tr><td>EDTA plasma(n=5)</td><td>83-99%</td><td>95</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-97%</td><td>91</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>91-102%</th><th>89-97%</th><th>86-102%</th><th>94-106%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>83-96%</td><td>95-103%</td><td>88-95%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-93%</td><td>88-92%</td><td>86-97%</td><td>90-101%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.068 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>1.961</td><td class='1'>1.875</td></tr><tr><td>5.00</td><td>1.520</td><td class='2'>1.434</td></tr><tr><td>2.50</td><td>1.121</td><td class='3'>1.035</td></tr><tr><td>1.25</td><td>0.907</td><td class='4'>0.821</td></tr><tr><td>0.63</td><td>0.492</td><td class='5'>0.406</td></tr><tr><td>0.32</td><td>0.389</td><td class='6'>0.303</td></tr><tr><td>0.16</td><td>0.211</td><td class='7'>0.125</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant MFN2 and the recovery rates were calculated by comparing the measured value to the expected amount of MFN2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-108%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>82-97%</td><td>89</td></tr><tr><td>Heparin plasma(n=5)</td><td>84-93%</td><td>87</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MFN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>83-92%</th><th>92-96%</th><th>86-98%</th><th>95-101%</th></tr><tr><td>EDTA plasma(n=5)</td><td>95-102%</td><td>87-101%</td><td>82-91%</td><td>85-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>93-102%</td><td>78-92%</td><td>87-98%</td><td>80-93%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.071 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.096</td><td class='1'>2.014</td></tr><tr><td>5.00</td><td>1.562</td><td class='2'>1.480</td></tr><tr><td>2.50</td><td>1.288</td><td class='3'>1.206</td></tr><tr><td>1.25</td><td>0.975</td><td class='4'>0.893</td></tr><tr><td>0.63</td><td>0.567</td><td class='5'>0.485</td></tr><tr><td>0.32</td><td>0.326</td><td class='6'>0.244</td></tr><tr><td>0.16</td><td>0.215</td><td class='7'>0.133</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant MFN1 and the recovery rates were calculated by comparing the measured value to the expected amount of MFN1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>82-96%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>86-97%</td><td>92</td></tr><tr><td>Heparin plasma(n=5)</td><td>84-103%</td><td>96</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MFN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-94%</th><th>93-101%</th><th>87-98%</th><th>89-102%</th></tr><tr><td>EDTA plasma(n=5)</td><td>81-94%</td><td>85-97%</td><td>93-102%</td><td>81-95%</td></tr><tr><td>Heparin plasma(n=5)</td><td>78-92%</td><td>82-96%</td><td>86-99%</td><td>93-101%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.14 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.126</td><td class='1'>2.029</td></tr><tr><td>10.00</td><td>1.602</td><td class='2'>1.505</td></tr><tr><td>5.00</td><td>1.133</td><td class='3'>1.036</td></tr><tr><td>2.50</td><td>0.907</td><td class='4'>0.810</td></tr><tr><td>1.25</td><td>0.541</td><td class='5'>0.444</td></tr><tr><td>0.63</td><td>0.302</td><td class='6'>0.205</td></tr><tr><td>0.32</td><td>0.175</td><td class='7'>0.078</td></tr><tr><td>0.00</td><td>0.097</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant JNK and the recovery rates were calculated by comparing the measured value to the expected amount of JNK in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>94-107%</td><td>100</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-95%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of JNK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>79-92%</th><th>88-96%</th><th>77-91%</th><th>85-93%</th></tr><tr><td>EDTA plasma(n=5)</td><td>95-103%</td><td>79-97%</td><td>93-102%</td><td>87-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-93%</td><td>79-96%</td><td>93-106%</td><td>87-99%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SQSTM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SQSTM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SQSTM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SQSTM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.121</td><td class='1'>2.032</td></tr><tr><td>5.00</td><td>1.631</td><td class='2'>1.542</td></tr><tr><td>2.50</td><td>1.277</td><td class='3'>1.188</td></tr><tr><td>1.25</td><td>0.797</td><td class='4'>0.708</td></tr><tr><td>0.63</td><td>0.547</td><td class='5'>0.458</td></tr><tr><td>0.32</td><td>0.265</td><td class='6'>0.176</td></tr><tr><td>0.16</td><td>0.165</td><td class='7'>0.076</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant SQSTM1 and the recovery rates were calculated by comparing the measured value to the expected amount of SQSTM1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>81-95%</td><td>88</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-99%</td><td>93</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SQSTM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>92-105%</th><th>83-92%</th><th>89-98%</th><th>85-94%</th></tr><tr><td>EDTA plasma(n=5)</td><td>79-93%</td><td>89-97%</td><td>85-94%</td><td>87-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-93%</td><td>85-95%</td><td>89-99%</td><td>85-95%</td></tr></tbody></table></div></div>

$588/96T $412/48T

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPTA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPTA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPTA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPTA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>5000.00</td><td>2.332</td><td class='1'>2.246</td></tr><tr><td>2500.00</td><td>1.720</td><td class='2'>1.634</td></tr><tr><td>1250.00</td><td>1.088</td><td class='3'>1.002</td></tr><tr><td>625.00</td><td>0.826</td><td class='4'>0.740</td></tr><tr><td>312.50</td><td>0.509</td><td class='5'>0.423</td></tr><tr><td>156.25</td><td>0.373</td><td class='6'>0.287</td></tr><tr><td>78.13</td><td>0.189</td><td class='7'>0.103</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant SPTA1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPTA1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>82-95%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>89-103%</td><td>96</td></tr><tr><td>Heparin plasma(n=5)</td><td>78-93%</td><td>85</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPTA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>81-93%</th><th>86-98%</th><th>91-103%</th><th>82-95%</th></tr><tr><td>EDTA plasma(n=5)</td><td>87-96%</td><td>91-101%</td><td>87-98%</td><td>89-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>84-94%</td><td>85-103%</td><td>94-105%</td><td>92-101%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.058 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep GAL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GAL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep GAL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GAL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.096</td><td class='1'>2.010</td></tr><tr><td>5.00</td><td>1.948</td><td class='2'>1.862</td></tr><tr><td>2.50</td><td>1.263</td><td class='3'>1.177</td></tr><tr><td>1.25</td><td>0.841</td><td class='4'>0.755</td></tr><tr><td>0.63</td><td>0.536</td><td class='5'>0.450</td></tr><tr><td>0.32</td><td>0.327</td><td class='6'>0.241</td></tr><tr><td>0.16</td><td>0.186</td><td class='7'>0.100</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant GAL3 and the recovery rates were calculated by comparing the measured value to the expected amount of GAL3 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>82-97%</td><td>89</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-108%</td><td>101</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAL3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-94%</th><th>87-99%</th><th>85-101%</th><th>79-90%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>87-96%</td><td>85-96%</td><td>85-95%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-95%</td><td>93-103%</td><td>87-98%</td><td>85-96%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.131 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep sST2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep sST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep sST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep sST2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.179</td><td class='1'>2.094</td></tr><tr><td>10.00</td><td>1.602</td><td class='2'>1.517</td></tr><tr><td>5.00</td><td>1.182</td><td class='3'>1.097</td></tr><tr><td>2.50</td><td>0.896</td><td class='4'>0.811</td></tr><tr><td>1.25</td><td>0.554</td><td class='5'>0.469</td></tr><tr><td>0.63</td><td>0.321</td><td class='6'>0.236</td></tr><tr><td>0.32</td><td>0.190</td><td class='7'>0.105</td></tr><tr><td>0.00</td><td>0.085</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant sST2 and the recovery rates were calculated by comparing the measured value to the expected amount of sST2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-102%</td><td>95</td></tr><tr><td>EDTA plasma(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-95%</td><td>87</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sST2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>78-92%</th><th>85-96%</th><th>93-102%</th><th>95-104%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-92%</td><td>79-96%</td><td>89-100%</td><td>85-96%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-95%</td><td>93-103%</td><td>85-94%</td><td>87-101%</td></tr></tbody></table></div></div>

$588/96T $412/48T

12.3 pg/mL

2000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TNNI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TNNI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TNNI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TNNI3 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>2000.00</td><td>2.126</td><td class='1'>2.037</td></tr><tr><td>1000.00</td><td>1.518</td><td class='2'>1.429</td></tr><tr><td>500.00</td><td>1.118</td><td class='3'>1.029</td></tr><tr><td>250.00</td><td>0.847</td><td class='4'>0.758</td></tr><tr><td>125.00</td><td>0.547</td><td class='5'>0.458</td></tr><tr><td>62.50</td><td>0.354</td><td class='6'>0.265</td></tr><tr><td>31.25</td><td>0.257</td><td class='7'>0.168</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant TNNI3 and the recovery rates were calculated by comparing the measured value to the expected amount of TNNI3 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>85-99%</td><td>92</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-99%</td><td>92</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNNI3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>86-97%</th><th>83-96%</th><th>91-103%</th><th>96-105%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-92%</td><td>79-96%</td><td>89-101%</td><td>85-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-100%</td><td>93-108%</td><td>80-101%</td><td>87-96%</td></tr></tbody></table></div></div>

$588/96T $412/48T

0.065 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.027</td><td class='1'>1.950</td></tr><tr><td>5.00</td><td>1.593</td><td class='2'>1.516</td></tr><tr><td>2.50</td><td>1.175</td><td class='3'>1.098</td></tr><tr><td>1.25</td><td>0.897</td><td class='4'>0.820</td></tr><tr><td>0.63</td><td>0.469</td><td class='5'>0.392</td></tr><tr><td>0.32</td><td>0.366</td><td class='6'>0.289</td></tr><tr><td>0.16</td><td>0.196</td><td class='7'>0.119</td></tr><tr><td>0.00</td><td>0.077</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>83-97%</td><td>90</td></tr><tr><td>EDTA plasma(n=5)</td><td>86-99%</td><td>92</td></tr><tr><td>Heparin plasma(n=5)</td><td>93-107%</td><td>100</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>86-95%</th><th>82-93%</th><th>97-106%</th><th>89-102%</th></tr><tr><td>EDTA plasma(n=5)</td><td>92-101%</td><td>82-98%</td><td>89-97%</td><td>85-93%</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-96%</td><td>93-104%</td><td>89-97%</td><td>85-94%</td></tr></tbody></table></div></div>

$588/96T $412/48T

13.5 pg/mL

2000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep BNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep BNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep BNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>2000.00</td><td>2.035</td><td class='1'>1.934</td></tr><tr><td>1000.00</td><td>1.536</td><td class='2'>1.435</td></tr><tr><td>500.00</td><td>1.135</td><td class='3'>1.034</td></tr><tr><td>250.00</td><td>0.933</td><td class='4'>0.832</td></tr><tr><td>125.00</td><td>0.591</td><td class='5'>0.490</td></tr><tr><td>62.50</td><td>0.318</td><td class='6'>0.217</td></tr><tr><td>31.25</td><td>0.288</td><td class='7'>0.187</td></tr><tr><td>0.00</td><td>0.101</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant BNP and the recovery rates were calculated by comparing the measured value to the expected amount of BNP in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>82-95%</td><td>88</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-97%</td><td>91</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>95-102%</th><th>89-96%</th><th>87-98%</th><th>85-93%</th></tr><tr><td>EDTA plasma(n=5)</td><td>86-95%</td><td>85-96%</td><td>89-100%</td><td>85-96%</td></tr><tr><td>Heparin plasma(n=5)</td><td>89-98%</td><td>83-103%</td><td>98-105%</td><td>92-101%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.075 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CARD9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CARD9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CARD9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CARD9 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.207</td><td class='1'>2.114</td></tr><tr><td>5.00</td><td>1.703</td><td class='2'>1.610</td></tr><tr><td>2.50</td><td>1.184</td><td class='3'>1.091</td></tr><tr><td>1.25</td><td>0.882</td><td class='4'>0.789</td></tr><tr><td>0.63</td><td>0.475</td><td class='5'>0.382</td></tr><tr><td>0.32</td><td>0.315</td><td class='6'>0.222</td></tr><tr><td>0.16</td><td>0.156</td><td class='7'>0.063</td></tr><tr><td>0.00</td><td>0.093</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant CARD9 and the recovery rates were calculated by comparing the measured value to the expected amount of CARD9 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>85-97%</td><td>91</td></tr><tr><td>EDTA plasma(n=5)</td><td>81-93%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-94%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CARD9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>95-102%</th><th>93-101%</th><th>81-89%</th><th>85-99%</th></tr><tr><td>EDTA plasma(n=5)</td><td>86-94%</td><td>85-95%</td><td>89-99%</td><td>85-95%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-96%</td><td>92-105%</td><td>89-102%</td><td>92-101%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.053 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPBAR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPBAR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPBAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPBAR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.043</td><td class='1'>1.958</td></tr><tr><td>5.00</td><td>1.602</td><td class='2'>1.517</td></tr><tr><td>2.50</td><td>1.184</td><td class='3'>1.099</td></tr><tr><td>1.25</td><td>0.852</td><td class='4'>0.767</td></tr><tr><td>0.63</td><td>0.553</td><td class='5'>0.468</td></tr><tr><td>0.32</td><td>0.315</td><td class='6'>0.230</td></tr><tr><td>0.16</td><td>0.168</td><td class='7'>0.083</td></tr><tr><td>0.00</td><td>0.085</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant GPBAR1 and the recovery rates were calculated by comparing the measured value to the expected amount of GPBAR1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>80-96%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>88-101%</td><td>94</td></tr><tr><td>Heparin plasma(n=5)</td><td>78-91%</td><td>84</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPBAR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>92-105%</th><th>85-101%</th><th>85-94%</th><th>87-96%</th></tr><tr><td>EDTA plasma(n=5)</td><td>79-96%</td><td>85-99%</td><td>79-93%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-97%</td><td>87-96%</td><td>85-96%</td><td>85-95%</td></tr></tbody></table></div></div>

$458/96T $320/48T

12.5 pg/mL

2000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TINAGL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TINAGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TINAGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TINAGL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>2000.00</td><td>2.075</td><td class='1'>1.987</td></tr><tr><td>1000.00</td><td>1.515</td><td class='2'>1.427</td></tr><tr><td>500.00</td><td>1.174</td><td class='3'>1.086</td></tr><tr><td>250.00</td><td>0.828</td><td class='4'>0.740</td></tr><tr><td>125.00</td><td>0.453</td><td class='5'>0.365</td></tr><tr><td>62.50</td><td>0.426</td><td class='6'>0.338</td></tr><tr><td>31.25</td><td>0.252</td><td class='7'>0.164</td></tr><tr><td>0.00</td><td>0.088</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant TINAGL1 and the recovery rates were calculated by comparing the measured value to the expected amount of TINAGL1 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>96-104%</td><td>100</td></tr><tr><td>Heparin plasma(n=5)</td><td>98-104%</td><td>102</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TINAGL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-94%</th><th>93-101%</th><th>87-98%</th><th>89-102%</th></tr><tr><td>EDTA plasma(n=5)</td><td>87-94%</td><td>85-96%</td><td>89-100%</td><td>85-96%</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-93%</td><td>95-104%</td><td>82-96%</td><td>85-98%</td></tr></tbody></table></div></div>

$458/96T $320/48T

29.3 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>5000.00</td><td>2.179</td><td class='1'>2.097</td></tr><tr><td>2500.00</td><td>1.579</td><td class='2'>1.497</td></tr><tr><td>1250.00</td><td>1.139</td><td class='3'>1.057</td></tr><tr><td>625.00</td><td>0.797</td><td class='4'>0.715</td></tr><tr><td>312.50</td><td>0.529</td><td class='5'>0.447</td></tr><tr><td>156.25</td><td>0.327</td><td class='6'>0.245</td></tr><tr><td>78.13</td><td>0.187</td><td class='7'>0.105</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant ATF4 and the recovery rates were calculated by comparing the measured value to the expected amount of ATF4 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>81-95%</td><td>88</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-96%</td><td>92</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-102%</th><th>83-96%</th><th>95-102%</th><th>87-98%</th></tr><tr><td>EDTA plasma(n=5)</td><td>86-100%</td><td>93-108%</td><td>80-101%</td><td>87-96%</td></tr><tr><td>Heparin plasma(n=5)</td><td>79-96%</td><td>85-99%</td><td>79-93%</td><td>89-97%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.11 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FUNDC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FUNDC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FUNDC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FUNDC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.058</td><td class='1'>1.961</td></tr><tr><td>10.00</td><td>1.412</td><td class='2'>1.315</td></tr><tr><td>5.00</td><td>1.128</td><td class='3'>1.031</td></tr><tr><td>2.50</td><td>0.864</td><td class='4'>0.767</td></tr><tr><td>1.25</td><td>0.473</td><td class='5'>0.376</td></tr><tr><td>0.63</td><td>0.324</td><td class='6'>0.227</td></tr><tr><td>0.32</td><td>0.179</td><td class='7'>0.082</td></tr><tr><td>0.00</td><td>0.097</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant FUNDC2 and the recovery rates were calculated by comparing the measured value to the expected amount of FUNDC2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>78-97%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>Heparin plasma(n=5)</td><td>83-97%</td><td>90</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FUNDC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>91-98%</th><th>83-101%</th><th>87-98%</th><th>95-102%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-103%</td><td>83-94%</td><td>92-105%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-106%</td><td>88-103%</td><td>81-98%</td><td>93-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

Zebrafish

0.21 ng/mL

40 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish AchE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish AchE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish AchE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish AchE in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>40.00</td><td>2.204</td><td class='1'>2.131</td></tr><tr><td>20.00</td><td>1.593</td><td class='2'>1.520</td></tr><tr><td>10.00</td><td>1.182</td><td class='3'>1.109</td></tr><tr><td>5.00</td><td>0.805</td><td class='4'>0.732</td></tr><tr><td>2.50</td><td>0.549</td><td class='5'>0.476</td></tr><tr><td>1.25</td><td>0.384</td><td class='6'>0.311</td></tr><tr><td>0.63</td><td>0.218</td><td class='7'>0.145</td></tr><tr><td>0.00</td><td>0.073</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant AchE and the recovery rates were calculated by comparing the measured value to the expected amount of AchE in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>80-97%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>87-99%</td><td>93</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-104%</td><td>97</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AchE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>93-105%</th><th>79-86%</th><th>87-103%</th><th>85-97%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-103%</td><td>83-94%</td><td>92-105%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-102%</td><td>85-94%</td><td>87-101%</td><td>89-99%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.051 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NCAM in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.075</td><td class='1'>1.967</td></tr><tr><td>5.00</td><td>1.515</td><td class='2'>1.407</td></tr><tr><td>2.50</td><td>1.088</td><td class='3'>0.980</td></tr><tr><td>1.25</td><td>0.882</td><td class='4'>0.774</td></tr><tr><td>0.63</td><td>0.509</td><td class='5'>0.401</td></tr><tr><td>0.32</td><td>0.239</td><td class='6'>0.131</td></tr><tr><td>0.16</td><td>0.187</td><td class='7'>0.079</td></tr><tr><td>0.00</td><td>0.108</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant NCAM and the recovery rates were calculated by comparing the measured value to the expected amount of NCAM in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-103%</td><td>96</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-93%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-99%</td><td>93</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-97%</th><th>87-96%</th><th>86-98%</th><th>91-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>83-94%</td><td>80-92%</td><td>86-102%</td><td>87-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>81-97%</td><td>88-102%</td><td>85-93%</td><td>83-97%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.049 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NCAM in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.126</td><td class='1'>2.044</td></tr><tr><td>5.00</td><td>1.579</td><td class='2'>1.497</td></tr><tr><td>2.50</td><td>1.067</td><td class='3'>0.985</td></tr><tr><td>1.25</td><td>0.867</td><td class='4'>0.785</td></tr><tr><td>0.63</td><td>0.529</td><td class='5'>0.447</td></tr><tr><td>0.32</td><td>0.323</td><td class='6'>0.241</td></tr><tr><td>0.16</td><td>0.249</td><td class='7'>0.167</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant NCAM and the recovery rates were calculated by comparing the measured value to the expected amount of NCAM in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>83-95%</td><td>89</td></tr><tr><td>EDTA plasma(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-97%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NCAM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>87-94%</th><th>85-96%</th><th>89-100%</th><th>85-96%</th></tr><tr><td>EDTA plasma(n=5)</td><td>86-105%</td><td>84-98%</td><td>95-102%</td><td>88-106%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-93%</td><td>91-103%</td><td>82-99%</td><td>99-104%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.23 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken VLDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken VLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken VLDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken VLDL in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>50.00</td><td>2.258</td><td class='1'>2.169</td></tr><tr><td>25.00</td><td>1.606</td><td class='2'>1.517</td></tr><tr><td>12.50</td><td>1.252</td><td class='3'>1.163</td></tr><tr><td>6.25</td><td>0.948</td><td class='4'>0.859</td></tr><tr><td>3.13</td><td>0.595</td><td class='5'>0.506</td></tr><tr><td>1.57</td><td>0.326</td><td class='6'>0.237</td></tr><tr><td>0.79</td><td>0.225</td><td class='7'>0.136</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant VLDL and the recovery rates were calculated by comparing the measured value to the expected amount of VLDL in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-102%</td><td>95</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-92%</td><td>86</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-105%</td><td>97</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VLDL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>88-97%</th><th>79-92%</th><th>85-93%</th><th>89-97%</th></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>87-96%</td><td>82-95%</td><td>92-105%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-97%</td><td>90-99%</td><td>82-101%</td><td>93-105%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.11 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AKT in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.379</td><td class='1'>2.290</td></tr><tr><td>10.00</td><td>1.565</td><td class='2'>1.476</td></tr><tr><td>5.00</td><td>1.196</td><td class='3'>1.107</td></tr><tr><td>2.50</td><td>0.797</td><td class='4'>0.708</td></tr><tr><td>1.25</td><td>0.508</td><td class='5'>0.419</td></tr><tr><td>0.63</td><td>0.336</td><td class='6'>0.247</td></tr><tr><td>0.32</td><td>0.198</td><td class='7'>0.109</td></tr><tr><td>0.00</td><td>0.089</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant AKT and the recovery rates were calculated by comparing the measured value to the expected amount of AKT in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>78-96%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-92%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-95%</td><td>87</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>88-97%</th><th>91-98%</th><th>86-103%</th><th>79-91%</th></tr><tr><td>EDTA plasma(n=5)</td><td>93-102%</td><td>87-96%</td><td>85-98%</td><td>91-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-96%</td><td>96-105%</td><td>81-93%</td><td>93-102%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.12 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>20.00</td><td>2.075</td><td class='1'>1.979</td></tr><tr><td>10.00</td><td>1.562</td><td class='2'>1.466</td></tr><tr><td>5.00</td><td>1.114</td><td class='3'>1.018</td></tr><tr><td>2.50</td><td>0.682</td><td class='4'>0.586</td></tr><tr><td>1.25</td><td>0.529</td><td class='5'>0.433</td></tr><tr><td>0.63</td><td>0.236</td><td class='6'>0.140</td></tr><tr><td>0.32</td><td>0.186</td><td class='7'>0.090</td></tr><tr><td>0.00</td><td>0.096</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>85-97%</td><td>91</td></tr><tr><td>EDTA plasma(n=5)</td><td>81-95%</td><td>88</td></tr><tr><td>Heparin plasma(n=5)</td><td>83-95%</td><td>89</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>79-91%</th><th>96-105%</th><th>80-92%</th><th>91-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-96%</td><td>86-95%</td><td>90-103%</td><td>87-90%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-94%</td><td>85-96%</td><td>92-102%</td><td>81-90%</td></tr></tbody></table></div></div>

$588/96T $412/48T

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>1000.00</td><td>2.166</td><td class='1'>2.064</td></tr><tr><td>500.00</td><td>1.593</td><td class='2'>1.491</td></tr><tr><td>250.00</td><td>1.157</td><td class='3'>1.055</td></tr><tr><td>125.00</td><td>0.883</td><td class='4'>0.781</td></tr><tr><td>62.50</td><td>0.567</td><td class='5'>0.465</td></tr><tr><td>31.25</td><td>0.336</td><td class='6'>0.234</td></tr><tr><td>15.63</td><td>0.186</td><td class='7'>0.084</td></tr><tr><td>0.00</td><td>0.102</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant IL4 and the recovery rates were calculated by comparing the measured value to the expected amount of IL4 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-103%</td><td>96</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-93%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-99%</td><td>93</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>88-102%</th><th>87-102%</th><th>85-96%</th><th>87-101%</th></tr><tr><td>EDTA plasma(n=5)</td><td>89-97%</td><td>85-94%</td><td>87-96%</td><td>85-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-94%</td><td>87-96%</td><td>92-101%</td><td>82-98%</td></tr></tbody></table></div></div>

$376/96T $265/48T

11.3 pg/mL

2000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>2000.00</td><td>1.979</td><td class='1'>1.893</td></tr><tr><td>1000.00</td><td>1.608</td><td class='2'>1.522</td></tr><tr><td>500.00</td><td>1.142</td><td class='3'>1.056</td></tr><tr><td>250.00</td><td>0.891</td><td class='4'>0.805</td></tr><tr><td>125.00</td><td>0.462</td><td class='5'>0.376</td></tr><tr><td>62.50</td><td>0.279</td><td class='6'>0.193</td></tr><tr><td>31.25</td><td>0.206</td><td class='7'>0.120</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant IL2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-93%</td><td>86</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>92-105%</th><th>83-92%</th><th>89-98%</th><th>85-94%</th></tr><tr><td>EDTA plasma(n=5)</td><td>89-98%</td><td>83-103%</td><td>98-105%</td><td>92-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-105%</td><td>87-98%</td><td>91-102%</td><td>86-102%</td></tr></tbody></table></div></div>

$588/96T $412/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.052</td><td class='1'>1.969</td></tr><tr><td>5.00</td><td>1.576</td><td class='2'>1.493</td></tr><tr><td>2.50</td><td>1.174</td><td class='3'>1.091</td></tr><tr><td>1.25</td><td>0.675</td><td class='4'>0.592</td></tr><tr><td>0.63</td><td>0.539</td><td class='5'>0.456</td></tr><tr><td>0.32</td><td>0.324</td><td class='6'>0.241</td></tr><tr><td>0.16</td><td>0.252</td><td class='7'>0.169</td></tr><tr><td>0.00</td><td>0.083</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant LEP and the recovery rates were calculated by comparing the measured value to the expected amount of LEP in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>86-99%</td><td>92</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>90-105%</td><td>97</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-92%</th><th>79-96%</th><th>89-100%</th><th>85-96%</th></tr><tr><td>EDTA plasma(n=5)</td><td>79-96%</td><td>85-99%</td><td>79-93%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-94%</td><td>87-101%</td><td>89-99%</td><td>85-95%</td></tr></tbody></table></div></div>

$588/96T $412/48T

4.81 pg/mL

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>1000.00</td><td>2.368</td><td class='1'>2.282</td></tr><tr><td>500.00</td><td>1.563</td><td class='2'>1.477</td></tr><tr><td>250.00</td><td>1.123</td><td class='3'>1.037</td></tr><tr><td>125.00</td><td>0.726</td><td class='4'>0.640</td></tr><tr><td>62.50</td><td>0.596</td><td class='5'>0.510</td></tr><tr><td>31.25</td><td>0.387</td><td class='6'>0.301</td></tr><tr><td>15.63</td><td>0.232</td><td class='7'>0.146</td></tr><tr><td>0.00</td><td>0.086</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>86-99%</td><td>92</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>86-97%</th><th>90-98%</th><th>80-93%</th><th>92-105%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-102%</td><td>87-102%</td><td>85-96%</td><td>87-101%</td></tr><tr><td>Heparin plasma(n=5)</td><td>87-94%</td><td>85-96%</td><td>89-100%</td><td>85-96%</td></tr></tbody></table></div></div>

$458/96T $320/48T

31.2 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIF5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIF5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIF5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIF5B in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>5000.00</td><td>2.027</td><td class='1'>1.931</td></tr><tr><td>2500.00</td><td>1.673</td><td class='2'>1.577</td></tr><tr><td>1250.00</td><td>1.243</td><td class='3'>1.147</td></tr><tr><td>625.00</td><td>0.935</td><td class='4'>0.839</td></tr><tr><td>312.50</td><td>0.573</td><td class='5'>0.477</td></tr><tr><td>156.25</td><td>0.315</td><td class='6'>0.219</td></tr><tr><td>78.13</td><td>0.258</td><td class='7'>0.162</td></tr><tr><td>0.00</td><td>0.096</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant KIF5B and the recovery rates were calculated by comparing the measured value to the expected amount of KIF5B in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>79-97%</td><td>88</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>84-98%</td><td>91</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KIF5B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>80-97%</th><th>81-92%</th><th>87-103%</th><th>82-93%</th></tr><tr><td>EDTA plasma(n=5)</td><td>91-103%</td><td>86-92%</td><td>88-97%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>79-94%</td><td>87-96%</td><td>78-93%</td><td>82-90%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.18 ng/mL

40 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LRP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LRP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LRP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LRP6 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>40.00</td><td>2.096</td><td class='1'>1.998</td></tr><tr><td>20.00</td><td>1.628</td><td class='2'>1.530</td></tr><tr><td>10.00</td><td>1.136</td><td class='3'>1.038</td></tr><tr><td>5.00</td><td>0.864</td><td class='4'>0.766</td></tr><tr><td>2.50</td><td>0.529</td><td class='5'>0.431</td></tr><tr><td>1.25</td><td>0.385</td><td class='6'>0.287</td></tr><tr><td>0.63</td><td>0.179</td><td class='7'>0.081</td></tr><tr><td>0.00</td><td>0.098</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant LRP6 and the recovery rates were calculated by comparing the measured value to the expected amount of LRP6 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>89-107%</td><td>98</td></tr><tr><td>EDTA plasma(n=5)</td><td>85-97%</td><td>91</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-94%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>87-98%</th><th>85-96%</th><th>92-102%</th><th>81-93%</th></tr><tr><td>EDTA plasma(n=5)</td><td>83-96%</td><td>86-95%</td><td>90-102%</td><td>81-93%</td></tr><tr><td>Heparin plasma(n=5)</td><td>83-94%</td><td>85-96%</td><td>92-103%</td><td>86-97%</td></tr></tbody></table></div></div>

$458/96T $320/48T

5.51 pg/mL

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SHH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SHH in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>1000.00</td><td>2.168</td><td class='1'>2.075</td></tr><tr><td>500.00</td><td>1.522</td><td class='2'>1.429</td></tr><tr><td>250.00</td><td>1.194</td><td class='3'>1.101</td></tr><tr><td>125.00</td><td>0.831</td><td class='4'>0.738</td></tr><tr><td>62.50</td><td>0.508</td><td class='5'>0.415</td></tr><tr><td>31.25</td><td>0.332</td><td class='6'>0.239</td></tr><tr><td>15.63</td><td>0.156</td><td class='7'>0.063</td></tr><tr><td>0.00</td><td>0.093</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant SHH and the recovery rates were calculated by comparing the measured value to the expected amount of SHH in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>89-103%</td><td>96</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-93%</td><td>85</td></tr><tr><td>Heparin plasma(n=5)</td><td>82-95%</td><td>88</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SHH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>96-105%</th><th>95-103%</th><th>95-103%</th><th>87-98%</th></tr><tr><td>EDTA plasma(n=5)</td><td>98-106%</td><td>86-102%</td><td>88-96%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>85-94%</td><td>87-96%</td><td>89-101%</td><td>82-90%</td></tr></tbody></table></div></div>

$458/96T $320/48T

2.12 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NUP62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NUP62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NUP62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NUP62 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (pg/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>5000.00</td><td>2.166</td><td class='1'>2.064</td></tr><tr><td>2500.00</td><td>1.593</td><td class='2'>1.491</td></tr><tr><td>1250.00</td><td>1.157</td><td class='3'>1.055</td></tr><tr><td>625.00</td><td>0.883</td><td class='4'>0.781</td></tr><tr><td>312.50</td><td>0.567</td><td class='5'>0.465</td></tr><tr><td>156.25</td><td>0.336</td><td class='6'>0.234</td></tr><tr><td>78.13</td><td>0.186</td><td class='7'>0.084</td></tr><tr><td>0.00</td><td>0.102</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant NUP62 and the recovery rates were calculated by comparing the measured value to the expected amount of NUP62 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>95-107%</td><td>101</td></tr><tr><td>EDTA plasma(n=5)</td><td>80-95%</td><td>87</td></tr><tr><td>Heparin plasma(n=5)</td><td>88-103%</td><td>95</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NUP62 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>91-99%</th><th>83-89%</th><th>88-104%</th><th>97-103%</th></tr><tr><td>EDTA plasma(n=5)</td><td>88-97%</td><td>96-103%</td><td>86-92%</td><td>89-97%</td></tr><tr><td>Heparin plasma(n=5)</td><td>80-101%</td><td>87-96%</td><td>96-105%</td><td>95-103%</td></tr></tbody></table></div></div>

$376/96T $265/48T

0.058 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LHCGR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LHCGR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LHCGR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LHCGR in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>2.165</td><td class='1'>2.078</td></tr><tr><td>5.00</td><td>1.563</td><td class='2'>1.476</td></tr><tr><td>2.50</td><td>1.196</td><td class='3'>1.109</td></tr><tr><td>1.25</td><td>0.831</td><td class='4'>0.744</td></tr><tr><td>0.63</td><td>0.559</td><td class='5'>0.472</td></tr><tr><td>0.32</td><td>0.377</td><td class='6'>0.290</td></tr><tr><td>0.16</td><td>0.216</td><td class='7'>0.129</td></tr><tr><td>0.00</td><td>0.087</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant LHCGR and the recovery rates were calculated by comparing the measured value to the expected amount of LHCGR in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>88-103%</td><td>95</td></tr><tr><td>EDTA plasma(n=5)</td><td>77-95%</td><td>86</td></tr><tr><td>Heparin plasma(n=5)</td><td>92-106%</td><td>99</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LHCGR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-97%</th><th>87-96%</th><th>85-96%</th><th>85-95%</th></tr><tr><td>EDTA plasma(n=5)</td><td>80-101%</td><td>87-96%</td><td>96-105%</td><td>95-103%</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-103%</td><td>87-98%</td><td>85-92%</td><td>79-96%</td></tr></tbody></table></div></div>

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PNPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PNPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PNPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PNPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

<div class='left fl'><table class='table3'><tbody><tr><th>Concentration (ng/mL) </th><th>OD</th><th>Corrected OD </th></tr><input type='hidden' name='' value='8'><tr><td>10.00</td><td>1.954</td><td class='1'>1.872</td></tr><tr><td>5.00</td><td>1.328</td><td class='2'>1.246</td></tr><tr><td>2.50</td><td>1.027</td><td class='3'>0.945</td></tr><tr><td>1.25</td><td>0.796</td><td class='4'>0.714</td></tr><tr><td>0.63</td><td>0.421</td><td class='5'>0.339</td></tr><tr><td>0.32</td><td>0.361</td><td class='6'>0.279</td></tr><tr><td>0.16</td><td>0.212</td><td class='7'>0.130</td></tr><tr><td>0.00</td><td>0.082</td><td class='8'>0.000</td></tr></tbody></table></div>

<div><div>Matrices listed below were spiked with certain level of recombinant PNPLA2 and the recovery rates were calculated by comparing the measured value to the expected amount of PNPLA2 in samples.</div><div><table><tbody><tr><th>Matrix</th><th>Recovery range</th><th>Average</th></tr><tr><td>serum(n=5)</td><td>90-105%</td><td>97</td></tr><tr><td>EDTA plasma(n=5)</td><td>78-90%</td><td>84</td></tr><tr><td>Heparin plasma(n=5)</td><td>95-107%</td><td>101</td></tr></tbody></table></div></div>

<div><div>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNPLA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.</div><div><table><tbody><tr><th>Matrix</th><th>1:2</th><th>1:4</th><th>1:8</th><th>1:16</th></tr<tr><th>serum(n=5)</th><th>85-96%</th><th>87-101%</th><th>89-102%</th><th>85-98%</th></tr><tr><td>EDTA plasma(n=5)</td><td>91-99%</td><td>88-97%</td><td>88-104%</td><td>97-108%</td></tr><tr><td>Heparin plasma(n=5)</td><td>93-104%</td><td>83-96%</td><td>95-103%</td><td>88-95%</td></tr></tbody></table></div></div>

$588/96T $412/48T

32.2 pg/mL

10000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

6.12 ng/mL

900 ng/mL

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

12.1 pg/mL

2000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse BDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.12 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF10 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

16.1 pg/mL

3000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARHGEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARHGEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARHGEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARHGEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.12 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGM5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGM5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGM5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGM5 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.059 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NUCB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NUCB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NUCB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NUCB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.047 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S1PR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S1PR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S1PR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S1PR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

21.3 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.116 ng/mL

0.116 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TBX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TBX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TBX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TBX5 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.68 ng/mL

100 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GATA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GATA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GATA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GATA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.22 ng/mL

40 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BTLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BTLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BTLA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BTLA in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

1.12 ng/mL

200 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OVA sIgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OVA sIgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OVA sIgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OVA sIgG in the samples is then determined by comparing the OD of the samples to the standard curve.

$588/96T $412/48T

Guinea pig

31.2 ng/mL

6000 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IgE in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.23 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse METRNL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse METRNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse METRNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse METRNL in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ECP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ECP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ECP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ECP in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

26.1 μg/mL

5000 μg/mL

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

500 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.32 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ORAI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ORAI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ORAI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ORAI3 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.13 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ORAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ORAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ORAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ORAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

31.2 pg/mL

5000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ORAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ORAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ORAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ORAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

0.184 mIU/mL

80 mIU/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hCG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hCG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hCG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hCG in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

0.184 mIU/mL

80 mIU/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse hCG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse hCG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse hCG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse hCG in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

0.12 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGCR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGCR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGCR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGCR in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

2.31 pg/mL

500 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.057 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIF3α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIF3α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIF3α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIF3α in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALHM6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CALHM6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CALHM6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALHM6 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.12 μIU/mL

20 μIU/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPRL in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.047 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TAN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TAN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.061 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SLC10A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SLC10A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SLC10A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SLC10A2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLb in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.11 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGFR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGFR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGFR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGFR4 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.12 ng/mL

40 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GPR131. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPR131. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GPR131, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GPR131 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

500 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IAPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.057 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RHOA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RHOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RHOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RHOA in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.067 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GLRX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GLRX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GLRX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GLRX in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.069 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken MUC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken MUC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken MUC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken MUC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.14 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANXA13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANXA13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ANXA13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANXA13 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.16 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

500 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgG4 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.68 ng/mL

100 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-NMDAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-NMDAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-NMDAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-NMDAR in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.067 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FOXO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FOXO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FOXO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FOXO1 in the samples is then determined by comparing the OD of the samples to the standard curve.

$458/96T $320/48T

0.65 ng/mL

100 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRAb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRAb in the samples is then determined by comparing the OD of the samples to the standard curve.

$376/96T $265/48T

1.49 ng/mL

300 ng/mL

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T4 in the samples is then determined by comparing the OD of the samples to the standard curve.