Bio 72A-D schedule fall 2019
 Share
The version of the browser you are using is no longer supported. Please upgrade to a supported browser.Dismiss

View only
 
 
ABCDEFGHIJKLMNOPQRSTUVWXYZ
1
Fall 2019 tentative schedule for Bio 72 A-D
2
Note: Subject to change! Please check back often.
3
BIO 72ABiotech Instrumentation: Good Laboratory Practices and Safe Chemical Handling
4
DateLectureLaboratory
5
8/20Introduction & overviewGood documentation practice; laboratory math
6
8/22Making solutions; working in a regulated environment part 1Making a gelatin solution for coating cell culture plates; working in a biological safety cabinet; Canvas F170A 5:00-6:00 PM
7
8/27Working in regulated environments part 2; online assessments 1, 2 dueChecking plates; gram staining; microscope usage; Promega buffers activity (solution prep)
8
8/29Working in regulated environments part 2 (cont.); math HW duePromega buffers activity (results);
9
9/3Hazard communication (MSDS section only); online assessments 3, 4 dueMaking BMM/BMG for yeast; cleaning validation; lab notebook check 1
10
9/5Validation: filtration and cleaning; Quiz 1Cleaning validation exercise, cont.; yeast inoculation BMG
11
9/10Counting cells; online assessment 5 dueYeast cell counts; yeast induction in BMM; safety check
12
9/12Filtration cont.; Quiz 2; case study dueGFP production check; autoclave validation; lab notebook check 2
13
14
BIO 72BBiotech Instrumentation: Clean Room
15
9/17Introduction to aseptic processingStage I (general) gowning; streak yeast on YPD plates for isolation
16
9/19Introduction to aseptic processing cont.M9 salts and minimal media for bacterial culture; BMG media for yeast bioreactor
17
9/24Clean Rooms part 1; online assessments 1 dueFed-batch, two-phase fermentation of E. coil - Day 1 (BFP production); OD600 using spectrophotometers
18
9/26Spectrophotometry & optical density; clean rooms cont.Fed-batch, two-phase fermentation of E. coli - Day 2
19
10/1Clean Room part 2; online assessments 3, 4 duePrepare bioreactors for scale up (assembly); TSA agar media prep for environmental monitoring
20
103Clean room behavior & environmental monitoring (EM) part 1Prepare media for bioreactors and autoclave; stage I gowning test; lab notebook check 1
Stage 1 and stage II (aseptic) gowning
21
10/8Environmental monitoring part 2; online assessment 5 dueBioreactor fermentation scale up and monitoring; particle counter; active air sampler; aseptic (stage 2) gowning qualification
22
10/10Review; Quiz 2; reading reflections due Friday by midnightBioreactor induction and monitoring; stage 2 gowning qualification results; lab notebook check 2
23
24
BIO 72CBiotech Instrumentation: PCR
25
10/15History of PCR & basic concepts; Fish PCR OverviewPCR master mix practice run & fish barcoding lesson 1 extraction (bring your fish samples -- see full manual on Canvas pg. 16 for best choices)
26
10/17Introduction to PCR: history of PCR and review of DNA replication and meaning of PCRFish barcoding lesson 2 PCR (ship sequencing samples next day); PTC taste genetics part 1 extraction & PCR
27
10/22Professional development day -- no classesProfessional development day -- no classes
28
10/24Guest Lecturer Ron Shigeta on Designing PCR for Protein Production; online assessments 1, 2 dueThe commercial uses of protein and some biology on how they are made. A lecture will be followed by a hands on lab where we learn how to use SnapGene Software to design PCR reactions, design primers, determine melting curves, and design plasmids for commercial protein production.
29
10/29Fish barcoding & sequencing technologyFish barcoding lesson 3 electrophoresis; PTC taste genetics part 2 digest & electrophoresis
30
10/31Human genes & PV92CSI lesson 1, 2; lab skill demo
31
11/5RT-PCR basics; online assessments 3, 4 dueCSI lesson 3, 4
32
11/7Fish PCR results; Quiz 1; lab notebook checkFish PCR Analysis in F170 2:30-4 pm
33
34
BIO 72DBiotech Instrumentation: Quality Control
35
11/12GENENTECH PILOT PLANT TOUR @ 2:30 pm22 DNA Way in South San Francisco, CA, be sure to arrive at least 15 minutes early!
36
11/14Introduction to qualityMaking quality Kool Aid; pouring BABEC plates
37
11/19QC microbiologyStart Phusion polymerase fed-batch; gram stain
38
11/21QC protein biochemistry; Making the Call quiz dueFed-batch induction of Phusion polymerase fermentation; cell lysis using lysis buffer
39
11/26QC protein biochemistry cont.; Take home Quiz 1; Quality 101 Ch. 1, 2 homework dueInsect cell culture media & YSI biochemistry analysis; bulk sterile fill using pumps
40
11/28Thanksgiving holiday
41
12/3QC chromatography; Quality 101 Ch. 3 homework due; Quiz 1 dueInsect cell counting and YSI
42
12/5QC chromatography cont.Insect cell monitoring & passaging; LP Biologic anion exchange chromatography
43
12/10QC chromatography cont.; reviewCleaning day; lab notebook check
44
12/12TBA lecture topic; Quiz 2 due; Quality 101 homework Ch. 9 due Friday, 12/14 by 5 pmTBA
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Loading...