DNA Technology quiz

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DNA Technology quiz

Take this quiz after you watch the DNA Technology video

Name *

DNA is ________________ charged so it will move in an electric field on a gel *

positively

negatively

Green Fluorescent Protein (GFP) is used to *

induce bacteria to take up plasmids

stain DNA fragments on gels

identify cells that contain recombinant plasmids

make copies of DNA using PCR

The enzyme used to cut DNA at specific sites for cloning genes is *

Taq polymerase

restriction endonuclease

reverse transcriptase

ligase

All of the following are TRUE about restriction endonucleases EXCEPT *

used to destroy "foreign DNA" in bacteria

can leave "sticky ends" when DNA is cut

can help amplify DNA using PCR

can be used to insert human DNA into bacteria

During DNA electrophoresis, fragments that are _________ move farther along the gel. *

shorter

longer

What is the source of restriction enzymes used in many DNA tenchologies? *

made in lab using reverse transcriptase

cloned from viral DNA

isolated from bacteria where they occur naturally

taken from eukaryotic cells and amplified by PCR

In genetic engineering, DNA ligase is used for which of the following purposes? *

create DNA from eukaryotic RNA

create breaks in plasmids to allow insertion of foreign DNA

join together fragments cut with endonucleases

act as a probe for locating cloned genes

In DNA technology a vector refers to *

a plasmid used to transfer DNA into a host cell

an RFLP marker

a radioactive probe used to identify a DNA sequence

a restriction site recognized by endonucleases

Which of the following is a problem with getting prokaryotic cells to express human genes? *

Their genetic codes differs because bacteria use uracil instead of thymine in DNA.

Prokaryiotic spliceosomes only recognize bacterial introns

Bacterial ribosomes are too small to translate long eukaryotic mRNA's

Bacterial cells lack splicing enzymes because they don't have introns

A scientist recovers a bit ot tissue from a 400 year old extinct Dodo bird and would like to compare it against samples from living birds. What technology would be most useful in increasing the amount of DNA for testing? *

bacterial transformation

polymerase chain reaction

RFLP analysis

electroporation

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