Single-cell assembly Group 2

	A	B	C	D	F	G	H	I
1	Group Members	Name	Function
2	1	Mona Högberg	HiSeq -> assembly
3	2	Bodil Cronholm	HiSeq -> trimming -> assembly
4	3	Muhammad Kashif	MiSeq -> trimming -> assembly
5	4	Tania Tajrin	MiSeq -> assembly
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7		Dataset 1	G5 HiSeq data			Dataset 2	G5 MiSeq data
8		No trimming				No trimming
9		Spades	IDBA-UD	Ray		Spades	IDBA-UD	Ray
10	Assembly time	11m8	0m14	3m19	Assembly time	14m	0m45
11	Number of reads				Number of reads		202058
12	Number of contigs				Number of contigs
13	Number of contigs >1kb				Number of contigs >1kb
14	Total assembly size				Total assembly size
15	Total assembly size >1kb				Total assembly size >1kb
16	Largest contig	27007	20216	42469	Largest contig
17	N50	8636	5528	13712	N50		750
18	G+C%				G+C%
19	Number of ORFs				Number of ORFs
20	Completeness				Completeness
21	# of contaminant contigs				# of contaminant contigs
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23
24		Trimming with Trimmomatic				Trimming with Trimmomatic
25		Spades	IDBA-UD	Ray		Spades	IDBA-UD	Ray
26	Assembly time	1:09	0:12	2:14	Assembly time	1m57	0m29	3m18
27	Number of reads (merged + unmerged pairs)	101855	101855	101855	Number of reads (merged + unmerged pairs)	2 x 101029	2 x 101029	2 x 101029
28	Number of contigs	76	79	91	Number of contigs	227	210	588
29	Number of contigs >1kb	47	50	50	Number of contigs >1kb	52	74	61
30	Total assembly size	310889	308118	212290	Total assembly size	425634	392106	398473
31	Total assembly size >1kb	291377	287902	184235	Total assembly size >1kb	346336	324985	260119
32	Largest contig	42469	26299	18700	Largest contig	43199	14203	20816
33	N50	13704	9273	4303	N50	14067	6224	7048
34	G+C%	40.37	40.42	39.86	G+C%	40.69	40.65	40.57
35	Number of ORFs	471	419	616	Number of ORFs	277	545	588
36	Completeness	0.1139	0.1136	0.0919	Completeness	0.1705	0.1487	0.1323
37	# of contaminant contigs				# of contaminant contigs
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39
40		Optional assembly optimization
41		Dataset 3	N21 MiSeq data
42	reads treatment
43	assembly program and parameters
44	Assembly time
45	Number of reads
46	Number of contigs
47	Total assembly size
48	Largest contig
49	N50
50	G+C%
51	Number of ORFs
52	Completeness
53	# of contaminant contigs
54
55	Total assembly size	177148
56	Largest contig	24892
57	N50	11747
58	G+C%	48.94
59	Number of ORFs	321
60	Completeness	0.0212
61	Markers found	2/139
62	# of contaminant contigs
63	16S identity	> AY861966.1.1230 Archaea;Euryarchaeota;Thermoplasmata;South African Goldmine Gp(SAGMEG);uncultured euryarchaeote
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