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Experimental stepsNecessary equipments/consumablesDIYbio solutionsSaving ratioReferences
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Cell cultureIncubator - 130$Styrofoam insulated box with heating pads, computer fans and a thermostat - recycled material - 10$13XS14http://hackteria.org/wiki/index.php/DIY_Incubator
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Bioreactor - 3000$Aquarium air pumps connected to plastic bottles with fluorescent tubes - recycled material - 100$30XS15http://hackteria.org/wiki/index.php/Algae_Culture_at_Home
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Microscopy400X optical microscope with camera - 130$Webcam optical microscope 400X - 10$13XS16http://hackteria.org/wiki/index.php/DIY_microscopy
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CentrifugesBenchtop centrifuge - 2000$Dremmelfuge (requires 3D printer, drill) - $10020XS17http://www.thingiverse.com/thing:1483
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Water bathWater bath - 400$Home-made water bath (aquarium heater + bucket) - 40$10XS18http://hackteria.org/wiki/index.php/DIY_Water_Bath
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Magnetic stirrerMagnetic stirrer - 70$DIY magnetic stirrer - 10$7XS19http://hackteria.org/wiki/index.php/Magnetic_stirrer
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SpectrophotometerSpectrophotometer - 150$DIY spectrophotometer - 10$15XS20http://publiclaboratory.org/tool/spectrometer
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Steriel workAutoclave - 500$Pressure cooker - 70$7XS21http://cathalgarvey.posterous.com/an-analysis-of-what-diybio-has-and-what-it-ne
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Microwave - 50$10XS22Sanborn, M. R., Wan, S. K. & Bulard, R. Microwave sterilization of plastic tissue culture vessels for reuse. Appl Environ Microbiol 44, 960–964 (1982).
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Sterile Hood - 1000$Custom sterile hood - 200$5XS23http://hackteria.org/wiki/index.php/DIY_Sterlisation_Hood
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ElectrophoresisGel Box - 400$ Home-made plastic Gel Box - $258XS24http://citizensciencequarterly.com/?p=3084&preview=true
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Pearl Gel Box Kit (with UV transilluminator) - 199$7XS25http://www.pearlbiotech.com/
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UV transilluminator - 1000$DIY UV transilluminator - 100$10XS26http://www.instructables.com/id/UV-Transilluminator/
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Power Supply - 1000$DIY Power Supply - 40$25XS27http://wiki.biohackers.la/index.php?title=Electrophoresis_Power_Supply&redirect=no
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PCRThermocycler - 2500$openPCR - 500$5XS28http://openpcr.org/
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Lava Amp - 300-500$ 5XS29http://www.lava-amp.com/
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Thermotyp - 400$6XS30http://speakscience.org/
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Personal PCR - 199$7XS31http://cofactorbio.com/personalpcr
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Bulb PCR - 25$100XS32http://russelldurrett.com/lightbulbpcr.html
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DNA purificationMiniprep kits - 1$ per miniprepDIY buffers + Regeneration of silica columns - reusable 10-20 times - 0.2$ per miniprep5XS33Siddappa, N., Avinash, A., Venkatramanan, M. & Ranga, U. Regeneration of commercial nucleic acid extraction columns without the risk of carryover contamination. BioTechniques 42, 186–192 (2007).
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DNA digestion / DNA Ligation / PCR/ DNA assemblyEnzymes - Taq polymerase 0.3$ per UnitDIY purification of recombinant proteins - Taq polymerase 0.001$ per Unit300XS34Grimm, E. & Arbuthnot, P. Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures. Nucleic Acids Res 23, 4518–4519 (1995).
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Make cells competent + transformationLong protocol using Ice and CaCl2 salts - Ice difficult to procureFast one-step method using PEG-3350 (laxative) + MgSO4 (Epsom salts) - Easy to find1XS35Chung, C., Niemela, S. L. & Miller, R. H. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86, 2172 (1989).
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Sequencing1 single read - 5-10$no cheaper alternative1XS36http://www.gatc-biotech.com/en/index.html
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