Group 3 140L

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V
1	vector		part	description	location in genome	sequence	length	forward oligo	reverse oligo	PCR sequence	cut sequence	part + vector sequence	cut with	Predicted PCR size	predicted cut sizes	Note		plasmid cut sizes with ncoI nad hindIII
2
3	pBad myc/hisA		TM-C	cysteine	741606..742988	ATGAGAATAACCAACACCCTGACAGGAAAAAAAGAAGAATTTGTGCCTATTCAACCCGGTGTTGTGAGGATGTACGTGTGCGGACCCACCGTGTACGATCTCATCCACGTGGGGAACGCGAGACCCGCGTTGGTATTCGATGTGTTCAGGAGGTACCTCGAGTACAGGGGTTACAGGGTCATAATGGTTCAGAATTTCACGGATATAGACGACAAGATCATAAACAAGGCGAATCAGCTCGGTGTCGATTACAAGACCGTGGCAGATACCTTCATAGCCGAGTACTGGAGAGACGCACATGCGCTTGGTATAAGACCGGCGAATTTTCATCCAAGAACCACCGATTTCGTTGAAGACATTGTTGAGATAATAGAAAAACTCGTAGAAAAAGGGTTCGCGTATCAAACGGAAACGGGTGTCTATTTCGATGTGAGAAAGTTCGAAAAGTACGGTGAACTCTCCAAAAAGAAGATAGAGGATCTCATCGCGGGTGCCAGAGTCGAGGTGGACGAAACGAAGAAGTCTCCTCTCGATTTCTCGCTCTGGAAAAAGGCCAAACCCGGTGAACCCTGCTGGAAGTCCCCCTGGGGAGAGGGAAGACCAGGTTGGCACATAGAGTGTACGGTTATGTCCGTGAAAATCCTGGGAGAGAGTTTCGATATACACGCGGGTGGAGAAGATCTCGTGTTTCCGCACCACGAGAACGAGAAGGCTCAGGCCGAAGCCCTGACCGGTAAGGTTTTTGCGAGATACTGGATGCACAACGGCATGGTCAGGTTCCTCGGTGATAAGATGAGTAAATCGACGGGAAACATCTTCACCGTCCGTGAAGCCGTGAAAAGATACGGCAGAGACGGTCTGAGATACATGATTCTCTCAAAGCACTACAGGTCGCCGATGGACTTCTCTGAAGAACTCCTTCAGGATTACTCCAGGGCGGTCAAACGTGTTTGGGAGATTCTGGGACGATACGAAAAATCTGGAGATATCGGGATACCTAAGAGAAACGCTGTTTACGAAGAGTACGTTAATCGTTTTGTGGAAGCTCTCGACGACGATTTCAACACACCAGTAGCGGTGTCCTTGATTTTTGAGCTCGCAAGAAACCTCAGCAAAGCGATGGATGACAACGACCGTGAGGATGCCCTTCTCTACTATCACCTGATCAGAAGGGAGTTCGGCCCCGTTCTGGGACTTTTCGATCTCAACGAAGAGAAGAAAGAAGTTTCCTCTGAAGAACTGCTCAAACTGCTGATAGAAGTGAGAGATGTCTTGAGGAAGGAAAAAAGATACGATCTTTCTGATCGAATAAGGGATCGCCTCAGGGAGATTGGAATCATTTTGAAGGATACCCCTTCGGGGACAGAATACACTGTCGAGTGA	1383	ccaaaCCATGGcgagaataaccaacaccctgacagg	tttggAAGCTTtcactcgacagtgtattctgtccc	ccaaaCCATGGcgAGAATAACCAACACCCTGACAGGAAAAAAAGAAGAATTTGTGCCTATTCAACCCGGTGTTGTGAGGATGTACGTGTGCGGACCCACCGTGTACGATCTCATCCACGTGGGGAACGCGAGACCCGCGTTGGTATTCGATGTGTTCAGGAGGTACCTCGAGTACAGGGGTTACAGGGTCATAATGGTTCAGAATTTCACGGATATAGACGACAAGATCATAAACAAGGCGAATCAGCTCGGTGTCGATTACAAGACCGTGGCAGATACCTTCATAGCCGAGTACTGGAGAGACGCACATGCGCTTGGTATAAGACCGGCGAATTTTCATCCAAGAACCACCGATTTCGTTGAAGACATTGTTGAGATAATAGAAAAACTCGTAGAAAAAGGGTTCGCGTATCAAACGGAAACGGGTGTCTATTTCGATGTGAGAAAGTTCGAAAAGTACGGTGAACTCTCCAAAAAGAAGATAGAGGATCTCATCGCGGGTGCCAGAGTCGAGGTGGACGAAACGAAGAAGTCTCCTCTCGATTTCTCGCTCTGGAAAAAGGCCAAACCCGGTGAACCCTGCTGGAAGTCCCCCTGGGGAGAGGGAAGACCAGGTTGGCACATAGAGTGTACGGTTATGTCCGTGAAAATCCTGGGAGAGAGTTTCGATATACACGCGGGTGGAGAAGATCTCGTGTTTCCGCACCACGAGAACGAGAAGGCTCAGGCCGAAGCCCTGACCGGTAAGGTTTTTGCGAGATACTGGATGCACAACGGCATGGTCAGGTTCCTCGGTGATAAGATGAGTAAATCGACGGGAAACATCTTCACCGTCCGTGAAGCCGTGAAAAGATACGGCAGAGACGGTCTGAGATACATGATTCTCTCAAAGCACTACAGGTCGCCGATGGACTTCTCTGAAGAACTCCTTCAGGATTACTCCAGGGCGGTCAAACGTGTTTGGGAGATTCTGGGACGATACGAAAAATCTGGAGATATCGGGATACCTAAGAGAAACGCTGTTTACGAAGAGTACGTTAATCGTTTTGTGGAAGCTCTCGACGACGATTTCAACACACCAGTAGCGGTGTCCTTGATTTTTGAGCTCGCAAGAAACCTCAGCAAAGCGATGGATGACAACGACCGTGAGGATGCCCTTCTCTACTATCACCTGATCAGAAGGGAGTTCGGCCCCGTTCTGGGACTTTTCGATCTCAACGAAGAGAAGAAAGAAGTTTCCTCTGAAGAACTGCTCAAACTGCTGATAGAAGTGAGAGATGTCTTGAGGAAGGAAAAAAGATACGATCTTTCTGATCGAATAAGGGATCGCCTCAGGGAGATTGGAATCATTTTGAAGGATACCCCTTCGGGGACAGAATACACTGTCGAGTGAAAGCTTccaaa	CATGGcgAGAATAACCAACACCCTGACAGGAAAAAAAGAAGAATTTGTGCCTATTCAACCCGGTGTTGTGAGGATGTACGTGTGCGGACCCACCGTGTACGATCTCATCCACGTGGGGAACGCGAGACCCGCGTTGGTATTCGATGTGTTCAGGAGGTACCTCGAGTACAGGGGTTACAGGGTCATAATGGTTCAGAATTTCACGGATATAGACGACAAGATCATAAACAAGGCGAATCAGCTCGGTGTCGATTACAAGACCGTGGCAGATACCTTCATAGCCGAGTACTGGAGAGACGCACATGCGCTTGGTATAAGACCGGCGAATTTTCATCCAAGAACCACCGATTTCGTTGAAGACATTGTTGAGATAATAGAAAAACTCGTAGAAAAAGGGTTCGCGTATCAAACGGAAACGGGTGTCTATTTCGATGTGAGAAAGTTCGAAAAGTACGGTGAACTCTCCAAAAAGAAGATAGAGGATCTCATCGCGGGTGCCAGAGTCGAGGTGGACGAAACGAAGAAGTCTCCTCTCGATTTCTCGCTCTGGAAAAAGGCCAAACCCGGTGAACCCTGCTGGAAGTCCCCCTGGGGAGAGGGAAGACCAGGTTGGCACATAGAGTGTACGGTTATGTCCGTGAAAATCCTGGGAGAGAGTTTCGATATACACGCGGGTGGAGAAGATCTCGTGTTTCCGCACCACGAGAACGAGAAGGCTCAGGCCGAAGCCCTGACCGGTAAGGTTTTTGCGAGATACTGGATGCACAACGGCATGGTCAGGTTCCTCGGTGATAAGATGAGTAAATCGACGGGAAACATCTTCACCGTCCGTGAAGCCGTGAAAAGATACGGCAGAGACGGTCTGAGATACATGATTCTCTCAAAGCACTACAGGTCGCCGATGGACTTCTCTGAAGAACTCCTTCAGGATTACTCCAGGGCGGTCAAACGTGTTTGGGAGATTCTGGGACGATACGAAAAATCTGGAGATATCGGGATACCTAAGAGAAACGCTGTTTACGAAGAGTACGTTAATCGTTTTGTGGAAGCTCTCGACGACGATTTCAACACACCAGTAGCGGTGTCCTTGATTTTTGAGCTCGCAAGAAACCTCAGCAAAGCGATGGATGACAACGACCGTGAGGATGCCCTTCTCTACTATCACCTGATCAGAAGGGAGTTCGGCCCCGTTCTGGGACTTTTCGATCTCAACGAAGAGAAGAAAGAAGTTTCCTCTGAAGAACTGCTCAAACTGCTGATAGAAGTGAGAGATGTCTTGAGGAAGGAAAAAAGATACGATCTTTCTGATCGAATAAGGGATCGCCTCAGGGAGATTGGAATCATTTTGAAGGATACCCCTTCGGGGACAGAATACACTGTCGAGTGAA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGcgAGAATAACCAACACCCTGACAGGAAAAAAAGAAGAATTTGTGCCTATTCAACCCGGTGTTGTGAGGATGTACGTGTGCGGACCCACCGTGTACGATCTCATCCACGTGGGGAACGCGAGACCCGCGTTGGTATTCGATGTGTTCAGGAGGTACCTCGAGTACAGGGGTTACAGGGTCATAATGGTTCAGAATTTCACGGATATAGACGACAAGATCATAAACAAGGCGAATCAGCTCGGTGTCGATTACAAGACCGTGGCAGATACCTTCATAGCCGAGTACTGGAGAGACGCACATGCGCTTGGTATAAGACCGGCGAATTTTCATCCAAGAACCACCGATTTCGTTGAAGACATTGTTGAGATAATAGAAAAACTCGTAGAAAAAGGGTTCGCGTATCAAACGGAAACGGGTGTCTATTTCGATGTGAGAAAGTTCGAAAAGTACGGTGAACTCTCCAAAAAGAAGATAGAGGATCTCATCGCGGGTGCCAGAGTCGAGGTGGACGAAACGAAGAAGTCTCCTCTCGATTTCTCGCTCTGGAAAAAGGCCAAACCCGGTGAACCCTGCTGGAAGTCCCCCTGGGGAGAGGGAAGACCAGGTTGGCACATAGAGTGTACGGTTATGTCCGTGAAAATCCTGGGAGAGAGTTTCGATATACACGCGGGTGGAGAAGATCTCGTGTTTCCGCACCACGAGAACGAGAAGGCTCAGGCCGAAGCCCTGACCGGTAAGGTTTTTGCGAGATACTGGATGCACAACGGCATGGTCAGGTTCCTCGGTGATAAGATGAGTAAATCGACGGGAAACATCTTCACCGTCCGTGAAGCCGTGAAAAGATACGGCAGAGACGGTCTGAGATACATGATTCTCTCAAAGCACTACAGGTCGCCGATGGACTTCTCTGAAGAACTCCTTCAGGATTACTCCAGGGCGGTCAAACGTGTTTGGGAGATTCTGGGACGATACGAAAAATCTGGAGATATCGGGATACCTAAGAGAAACGCTGTTTACGAAGAGTACGTTAATCGTTTTGTGGAAGCTCTCGACGACGATTTCAACACACCAGTAGCGGTGTCCTTGATTTTTGAGCTCGCAAGAAACCTCAGCAAAGCGATGGATGACAACGACCGTGAGGATGCCCTTCTCTACTATCACCTGATCAGAAGGGAGTTCGGCCCCGTTCTGGGACTTTTCGATCTCAACGAAGAGAAGAAAGAAGTTTCCTCTGAAGAACTGCTCAAACTGCTGATAGAAGTGAGAGATGTCTTGAGGAAGGAAAAAAGATACGATCTTTCTGATCGAATAAGGGATCGCCTCAGGGAGATTGGAATCATTTTGAAGGATACCCCTTCGGGGACAGAATACACTGTCGAGTGAAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	1404	1388	insert ala at start	cysteine	4, 1.4
4	pBad myc/hisA		TM-D	aspartic acid	1456160..1457899	ATGCTGAGAACACACACGTGTGGAGAGCTGAGAGCAACAGACGAGGGAAAAAAAGTGAAGCTGTGCGGATGGGTGGACAGGATCAGAGATCTCGGTGGTGTGAGATTCATCGACTTGAGAGACAGATACGGTGAGACTCAAATCGTGTGCGATGTGAACAGTGAGGCTTACAGTGTTGTAGATGAACTCACAAGAGAATCCGTCGTTCTGGTTGAGGGAACCGTGAGAAAAAGGCCAGAAGGGACGGAAAATCCGAACATCGAAACAGGAGAAATAGAAGTCGTCGCCGAAAGAATAGAGATCCTGTCTCTTGCCGATCCGCTTCCTTTTTATCCTGGAGAAACACCTAAAGAAGAAATGAGACTCAAGTACAGATACATAGATCTTCGTTCCGAGAGAATGAAGAGAAATATCATTTTGAGATACAGAATATCGAAAATCATCAGAGATTACTTTGATGAACTCGGTTTCCTTGAAATAGAAACTCCCTTTCTCACAAGAAGCACTCCGGAAGGGGCGAGGGATTTCCTCGTACCTTCGAGACTCAGACCCGGAAAGTTCTACGCGCTGCCTCAATCTCCTCAGCTCTTCAAGCAGATTCTGATGATCTCCGGCTTCGACAGATACTTCCAGATAGTCAGATGCTTCAGGGACGAAGACCTCAGAGCAGACAGGCAACCGGAGTTCACCCAGGTTGACGTGGAGATGTCCTTTGTCGATGTGGAAGATGTACTGAATGTGAGCGAAGGAATGGTTTCAAGAGTGTTCAAAGAATCTTCTGGCATCGATCTCAAGGTTCCCTTCGACAGAATTCCGTACGACGATGCGATGGAAAAGTATGGAACAGACAAGCCAGACAGAAGATACGGAATGGAACTTCGAGATTTTGGATACGCTTTTGAGACCACGGAGTTCAAGGTGATAAGGAACGTCCTCAATGAAGGAGGAAGTGTGAAGGGTTTCATCGTTCCCGGATTCGCTTCGGAGATGAGCAGAAAGAAAGGCGAAGAACTCATGGCAAGAATGAAAGAACTCGGTCTCGGCGGTCTCATCTGGTTCAAATTGGACGGTGGAATCACGTCACCACACCTGAAACACCTCGAAAAAGAATTCAGAAAGATCGCAGAAACGGAAAACATGAACGAAGGAGACGTCTGTCTCATAGCGGCCCACACTGATAGAAACCTCCTCAACGAAGCCCTTGGAACGCTGAGGCTGGAAATCGGAAAAGAGCACTTCTCACACCTTGCAAAAGGATTCGATGTACTCTGGGTAGTCGACTTTCCATACTTCGAGTGGAGCGAGGAAGAAGAAAGATTTGTGGCGCGGCACCACCCCTTCACAATGCCCGTTCTGGAAACCTTAGGTGATGATTACACAAAGGTCCGGGCGAAAGCCTACGATCTCGTCATCAACGGTTACGAAGTCGGTGGAGGAAGCATCAGGATTCACAGAAGAGATATTCAGGAGAAAATCTTTGAACTTCTTGGGTTGAGTGAAGAGGAAGCACAGAAAAAATTCGGCTTCTTCCTCGAAGCGTTCAGGTACGGCGTTCCGCCGCACGGCGGGATAGCTTTCGGACTGGATCGATTGGTGAGCATCATAGCCGGTGAGAGTTCTATAAGAGAAGTGATAGCGTTTCCAAAAACAGGAAACGGTGTGTGCCTCTTGACGGGAGCCCCCGCTGAAGTTGATGAAAGGCAACTCAGAGAACTCAGAATTAGAATAGAGGAGGGATAA	1740	ccaaaCCATGGccctgagaacacacacgtgtggagagc	tttggAAGCTTttatccctcctctattctaattctg	ccaaaCCATGGccCTGAGAACACACACGTGTGGAGAGCTGAGAGCAACAGACGAGGGAAAAAAAGTGAAGCTGTGCGGATGGGTGGACAGGATCAGAGATCTCGGTGGTGTGAGATTCATCGACTTGAGAGACAGATACGGTGAGACTCAAATCGTGTGCGATGTGAACAGTGAGGCTTACAGTGTTGTAGATGAACTCACAAGAGAATCCGTCGTTCTGGTTGAGGGAACCGTGAGAAAAAGGCCAGAAGGGACGGAAAATCCGAACATCGAAACAGGAGAAATAGAAGTCGTCGCCGAAAGAATAGAGATCCTGTCTCTTGCCGATCCGCTTCCTTTTTATCCTGGAGAAACACCTAAAGAAGAAATGAGACTCAAGTACAGATACATAGATCTTCGTTCCGAGAGAATGAAGAGAAATATCATTTTGAGATACAGAATATCGAAAATCATCAGAGATTACTTTGATGAACTCGGTTTCCTTGAAATAGAAACTCCCTTTCTCACAAGAAGCACTCCGGAAGGGGCGAGGGATTTCCTCGTACCTTCGAGACTCAGACCCGGAAAGTTCTACGCGCTGCCTCAATCTCCTCAGCTCTTCAAGCAGATTCTGATGATCTCCGGCTTCGACAGATACTTCCAGATAGTCAGATGCTTCAGGGACGAAGACCTCAGAGCAGACAGGCAACCGGAGTTCACCCAGGTTGACGTGGAGATGTCCTTTGTCGATGTGGAAGATGTACTGAATGTGAGCGAAGGAATGGTTTCAAGAGTGTTCAAAGAATCTTCTGGCATCGATCTCAAGGTTCCCTTCGACAGAATTCCGTACGACGATGCGATGGAAAAGTATGGAACAGACAAGCCAGACAGAAGATACGGAATGGAACTTCGAGATTTTGGATACGCTTTTGAGACCACGGAGTTCAAGGTGATAAGGAACGTCCTCAATGAAGGAGGAAGTGTGAAGGGTTTCATCGTTCCCGGATTCGCTTCGGAGATGAGCAGAAAGAAAGGCGAAGAACTCATGGCAAGAATGAAAGAACTCGGTCTCGGCGGTCTCATCTGGTTCAAATTGGACGGTGGAATCACGTCACCACACCTGAAACACCTCGAAAAAGAATTCAGAAAGATCGCAGAAACGGAAAACATGAACGAAGGAGACGTCTGTCTCATAGCGGCCCACACTGATAGAAACCTCCTCAACGAAGCCCTTGGAACGCTGAGGCTGGAAATCGGAAAAGAGCACTTCTCACACCTTGCAAAAGGATTCGATGTACTCTGGGTAGTCGACTTTCCATACTTCGAGTGGAGCGAGGAAGAAGAAAGATTTGTGGCGCGGCACCACCCCTTCACAATGCCCGTTCTGGAAACCTTAGGTGATGATTACACAAAGGTCCGGGCGAAAGCCTACGATCTCGTCATCAACGGTTACGAAGTCGGTGGAGGAAGCATCAGGATTCACAGAAGAGATATTCAGGAGAAAATCTTTGAACTTCTTGGGTTGAGTGAAGAGGAAGCACAGAAAAAATTCGGCTTCTTCCTCGAAGCGTTCAGGTACGGCGTTCCGCCGCACGGCGGGATAGCTTTCGGACTGGATCGATTGGTGAGCATCATAGCCGGTGAGAGTTCTATAAGAGAAGTGATAGCGTTTCCAAAAACAGGAAACGGTGTGTGCCTCTTGACGGGAGCCCCCGCTGAAGTTGATGAAAGGCAACTCAGAGAACTCAGAATTAGAATAGAGGAGGGATAAcagaattagaatagaggagggataaAAGCTTccaaa	CATGGccCTGAGAACACACACGTGTGGAGAGCTGAGAGCAACAGACGAGGGAAAAAAAGTGAAGCTGTGCGGATGGGTGGACAGGATCAGAGATCTCGGTGGTGTGAGATTCATCGACTTGAGAGACAGATACGGTGAGACTCAAATCGTGTGCGATGTGAACAGTGAGGCTTACAGTGTTGTAGATGAACTCACAAGAGAATCCGTCGTTCTGGTTGAGGGAACCGTGAGAAAAAGGCCAGAAGGGACGGAAAATCCGAACATCGAAACAGGAGAAATAGAAGTCGTCGCCGAAAGAATAGAGATCCTGTCTCTTGCCGATCCGCTTCCTTTTTATCCTGGAGAAACACCTAAAGAAGAAATGAGACTCAAGTACAGATACATAGATCTTCGTTCCGAGAGAATGAAGAGAAATATCATTTTGAGATACAGAATATCGAAAATCATCAGAGATTACTTTGATGAACTCGGTTTCCTTGAAATAGAAACTCCCTTTCTCACAAGAAGCACTCCGGAAGGGGCGAGGGATTTCCTCGTACCTTCGAGACTCAGACCCGGAAAGTTCTACGCGCTGCCTCAATCTCCTCAGCTCTTCAAGCAGATTCTGATGATCTCCGGCTTCGACAGATACTTCCAGATAGTCAGATGCTTCAGGGACGAAGACCTCAGAGCAGACAGGCAACCGGAGTTCACCCAGGTTGACGTGGAGATGTCCTTTGTCGATGTGGAAGATGTACTGAATGTGAGCGAAGGAATGGTTTCAAGAGTGTTCAAAGAATCTTCTGGCATCGATCTCAAGGTTCCCTTCGACAGAATTCCGTACGACGATGCGATGGAAAAGTATGGAACAGACAAGCCAGACAGAAGATACGGAATGGAACTTCGAGATTTTGGATACGCTTTTGAGACCACGGAGTTCAAGGTGATAAGGAACGTCCTCAATGAAGGAGGAAGTGTGAAGGGTTTCATCGTTCCCGGATTCGCTTCGGAGATGAGCAGAAAGAAAGGCGAAGAACTCATGGCAAGAATGAAAGAACTCGGTCTCGGCGGTCTCATCTGGTTCAAATTGGACGGTGGAATCACGTCACCACACCTGAAACACCTCGAAAAAGAATTCAGAAAGATCGCAGAAACGGAAAACATGAACGAAGGAGACGTCTGTCTCATAGCGGCCCACACTGATAGAAACCTCCTCAACGAAGCCCTTGGAACGCTGAGGCTGGAAATCGGAAAAGAGCACTTCTCACACCTTGCAAAAGGATTCGATGTACTCTGGGTAGTCGACTTTCCATACTTCGAGTGGAGCGAGGAAGAAGAAAGATTTGTGGCGCGGCACCACCCCTTCACAATGCCCGTTCTGGAAACCTTAGGTGATGATTACACAAAGGTCCGGGCGAAAGCCTACGATCTCGTCATCAACGGTTACGAAGTCGGTGGAGGAAGCATCAGGATTCACAGAAGAGATATTCAGGAGAAAATCTTTGAACTTCTTGGGTTGAGTGAAGAGGAAGCACAGAAAAAATTCGGCTTCTTCCTCGAAGCGTTCAGGTACGGCGTTCCGCCGCACGGCGGGATAGCTTTCGGACTGGATCGATTGGTGAGCATCATAGCCGGTGAGAGTTCTATAAGAGAAGTGATAGCGTTTCCAAAAACAGGAAACGGTGTGTGCCTCTTGACGGGAGCCCCCGCTGAAGTTGATGAAAGGCAACTCAGAGAACTCAGAATTAGAATAGAGGAGGGATAAcagaattagaatagaggagggataaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGccCTGAGAACACACACGTGTGGAGAGCTGAGAGCAACAGACGAGGGAAAAAAAGTGAAGCTGTGCGGATGGGTGGACAGGATCAGAGATCTCGGTGGTGTGAGATTCATCGACTTGAGAGACAGATACGGTGAGACTCAAATCGTGTGCGATGTGAACAGTGAGGCTTACAGTGTTGTAGATGAACTCACAAGAGAATCCGTCGTTCTGGTTGAGGGAACCGTGAGAAAAAGGCCAGAAGGGACGGAAAATCCGAACATCGAAACAGGAGAAATAGAAGTCGTCGCCGAAAGAATAGAGATCCTGTCTCTTGCCGATCCGCTTCCTTTTTATCCTGGAGAAACACCTAAAGAAGAAATGAGACTCAAGTACAGATACATAGATCTTCGTTCCGAGAGAATGAAGAGAAATATCATTTTGAGATACAGAATATCGAAAATCATCAGAGATTACTTTGATGAACTCGGTTTCCTTGAAATAGAAACTCCCTTTCTCACAAGAAGCACTCCGGAAGGGGCGAGGGATTTCCTCGTACCTTCGAGACTCAGACCCGGAAAGTTCTACGCGCTGCCTCAATCTCCTCAGCTCTTCAAGCAGATTCTGATGATCTCCGGCTTCGACAGATACTTCCAGATAGTCAGATGCTTCAGGGACGAAGACCTCAGAGCAGACAGGCAACCGGAGTTCACCCAGGTTGACGTGGAGATGTCCTTTGTCGATGTGGAAGATGTACTGAATGTGAGCGAAGGAATGGTTTCAAGAGTGTTCAAAGAATCTTCTGGCATCGATCTCAAGGTTCCCTTCGACAGAATTCCGTACGACGATGCGATGGAAAAGTATGGAACAGACAAGCCAGACAGAAGATACGGAATGGAACTTCGAGATTTTGGATACGCTTTTGAGACCACGGAGTTCAAGGTGATAAGGAACGTCCTCAATGAAGGAGGAAGTGTGAAGGGTTTCATCGTTCCCGGATTCGCTTCGGAGATGAGCAGAAAGAAAGGCGAAGAACTCATGGCAAGAATGAAAGAACTCGGTCTCGGCGGTCTCATCTGGTTCAAATTGGACGGTGGAATCACGTCACCACACCTGAAACACCTCGAAAAAGAATTCAGAAAGATCGCAGAAACGGAAAACATGAACGAAGGAGACGTCTGTCTCATAGCGGCCCACACTGATAGAAACCTCCTCAACGAAGCCCTTGGAACGCTGAGGCTGGAAATCGGAAAAGAGCACTTCTCACACCTTGCAAAAGGATTCGATGTACTCTGGGTAGTCGACTTTCCATACTTCGAGTGGAGCGAGGAAGAAGAAAGATTTGTGGCGCGGCACCACCCCTTCACAATGCCCGTTCTGGAAACCTTAGGTGATGATTACACAAAGGTCCGGGCGAAAGCCTACGATCTCGTCATCAACGGTTACGAAGTCGGTGGAGGAAGCATCAGGATTCACAGAAGAGATATTCAGGAGAAAATCTTTGAACTTCTTGGGTTGAGTGAAGAGGAAGCACAGAAAAAATTCGGCTTCTTCCTCGAAGCGTTCAGGTACGGCGTTCCGCCGCACGGCGGGATAGCTTTCGGACTGGATCGATTGGTGAGCATCATAGCCGGTGAGAGTTCTATAAGAGAAGTGATAGCGTTTCCAAAAACAGGAAACGGTGTGTGCCTCTTGACGGGAGCCCCCGCTGAAGTTGATGAAAGGCAACTCAGAGAACTCAGAATTAGAATAGAGGAGGGATAAcagaattagaatagaggagggataaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	1786	1770	insert ala at start	aspartic acid	4, 1.8
5	pBad myc/hisA		TM-E (1)	glutamic acid	1855907..1857370	gtgttcatcaccggggctttttttgatatattagaagtcggaccaaaaaaaatcaggaggtgcttcgaattggtcagagtcaggttcgccccgagtccaacaggccatctccacgtaggtggagccagaaccgctctgttcaactggatgttcgcaagaaaagagggtggaaagtttatccttcgaatagaagacaccgatactgagagaagctcaagagaatacgaacagcagatcctggaatctttgaggtggtgcggtctcgattgggacgaagggcctgacataggtggagatttcggaccctatcgacagagcgaaagactcgagatctaccgagaatacgctgagaaactggtggaagataagagggcttattacgtggtttacgataaggaagatccctctaaagagctcttcacaacttacgagtatccccacgaatacaaggagaaagggcatcccgttaccataaaattcaaagttcttcccggaaagacttccttcgaggatcttctgaaaggctacatggaattcgataactccaccttagaagatttcatcatcatgaagtcgaacggatttcccacctataattttgcggtcgtggtggacgatcatctcatgagaatctcccatgtgtttcgcggtgaagatcacctttctaacaccccaaaacagctcatgatatacgaggctttcggatgggaagcacccgtcttcatgcacattccactcatccttggatcggacagaacgcctttgagtaaaagacatggcgccacttccgttgaacacttcagaagggagggcattttgagcagggctttgatgaactatcttgcacttctcggatggagagtggaaggagacgaaatcttcactatagaagagaaacttcagtcattcgatccaaaagatatttcgaacaagggagtcatattcgattaccagaaactcgaatgggtgaacggaaagcacatgagaagaatcgatctcgaagacctgaagagggaattcatcgaatgggcaaagtacgcgggaaaagaaatcccctccgtggatgaaagatacttctcagagaccctccgtatatgtcgggaaaaggtgaacacactctctcagctgtacgacatcatgtatccgttcatgaacgacgattacgaatacgaaaaggactatgtagagaaatttttgaagagagaagaagccgaaagggtgcttgaagaagcaaaaaaagccttcaaagatctcaacagctggaatatggaagagatagaaaaaacattgagagatctttctgaaaagggtctggcatcgaaaaaggtggtctttcagctcataagaggagcggttactggaaagctggtaacacccggtctttttgaaaccatagaggtgctgggaaaagaaagaacactgaaaagacttgaaagaactttacagttcttaaagaaaacctaa	1464	ccaaaCCATGGgcttcatcaccggggctttttttg	tttggAAGCTTttaggttttctttaagaactgtaaag	ccaaaCCATGGgcTTCATCACCGGGGCTTTTTTTGATATATTAGAAGTCGGACCAAAAAAAATCAGGAGGTGCTTCGAATTGGTCAGAGTCAGGTTCGCCCCGAGTCCAACAGGCCATCTCCACGTAGGTGGAGCCAGAACCGCTCTGTTCAACTGGATGTTCGCAAGAAAAGAGGGTGGAAAGTTTATCCTTCGAATAGAAGACACCGATACTGAGAGAAGCTCAAGAGAATACGAACAGCAGATCCTGGAATCTTTGAGGTGGTGCGGTCTCGATTGGGACGAAGGGCCTGACATAGGTGGAGATTTCGGACCCTATCGACAGAGCGAAAGACTCGAGATCTACCGAGAATACGCTGAGAAACTGGTGGAAGATAAGAGGGCTTATTACGTGGTTTACGATAAGGAAGATCCCTCTAAAGAGCTCTTCACAACTTACGAGTATCCCCACGAATACAAGGAGAAAGGGCATCCCGTTACCATAAAATTCAAAGTTCTTCCCGGAAAGACTTCCTTCGAGGATCTTCTGAAAGGCTACATGGAATTCGATAACTCCACCTTAGAAGATTTCATCATCATGAAGTCGAACGGATTTCCCACCTATAATTTTGCGGTCGTGGTGGACGATCATCTCATGAGAATCTCCCATGTGTTTCGCGGTGAAGATCACCTTTCTAACACCCCAAAACAGCTCATGATATACGAGGCTTTCGGATGGGAAGCACCCGTCTTCATGCACATTCCACTCATCCTTGGATCGGACAGAACGCCTTTGAGTAAAAGACATGGCGCCACTTCCGTTGAACACTTCAGAAGGGAGGGCATTTTGAGCAGGGCTTTGATGAACTATCTTGCACTTCTCGGATGGAGAGTGGAAGGAGACGAAATCTTCACTATAGAAGAGAAACTTCAGTCATTCGATCCAAAAGATATTTCGAACAAGGGAGTCATATTCGATTACCAGAAACTCGAATGGGTGAACGGAAAGCACATGAGAAGAATCGATCTCGAAGACCTGAAGAGGGAATTCATCGAATGGGCAAAGTACGCGGGAAAAGAAATCCCCTCCGTGGATGAAAGATACTTCTCAGAGACCCTCCGTATATGTCGGGAAAAGGTGAACACACTCTCTCAGCTGTACGACATCATGTATCCGTTCATGAACGACGATTACGAATACGAAAAGGACTATGTAGAGAAATTTTTGAAGAGAGAAGAAGCCGAAAGGGTGCTTGAAGAAGCAAAAAAAGCCTTCAAAGATCTCAACAGCTGGAATATGGAAGAGATAGAAAAAACATTGAGAGATCTTTCTGAAAAGGGTCTGGCATCGAAAAAGGTGGTCTTTCAGCTCATAAGAGGAGCGGTTACTGGAAAGCTGGTAACACCCGGTCTTTTTGAAACCATAGAGGTGCTGGGAAAAGAAAGAACACTGAAAAGACTTGAAAGAActttacagttcttaaagaaaacctaaAAGCTTccaaa	CATGGgcTTCATCACCGGGGCTTTTTTTGATATATTAGAAGTCGGACCAAAAAAAATCAGGAGGTGCTTCGAATTGGTCAGAGTCAGGTTCGCCCCGAGTCCAACAGGCCATCTCCACGTAGGTGGAGCCAGAACCGCTCTGTTCAACTGGATGTTCGCAAGAAAAGAGGGTGGAAAGTTTATCCTTCGAATAGAAGACACCGATACTGAGAGAAGCTCAAGAGAATACGAACAGCAGATCCTGGAATCTTTGAGGTGGTGCGGTCTCGATTGGGACGAAGGGCCTGACATAGGTGGAGATTTCGGACCCTATCGACAGAGCGAAAGACTCGAGATCTACCGAGAATACGCTGAGAAACTGGTGGAAGATAAGAGGGCTTATTACGTGGTTTACGATAAGGAAGATCCCTCTAAAGAGCTCTTCACAACTTACGAGTATCCCCACGAATACAAGGAGAAAGGGCATCCCGTTACCATAAAATTCAAAGTTCTTCCCGGAAAGACTTCCTTCGAGGATCTTCTGAAAGGCTACATGGAATTCGATAACTCCACCTTAGAAGATTTCATCATCATGAAGTCGAACGGATTTCCCACCTATAATTTTGCGGTCGTGGTGGACGATCATCTCATGAGAATCTCCCATGTGTTTCGCGGTGAAGATCACCTTTCTAACACCCCAAAACAGCTCATGATATACGAGGCTTTCGGATGGGAAGCACCCGTCTTCATGCACATTCCACTCATCCTTGGATCGGACAGAACGCCTTTGAGTAAAAGACATGGCGCCACTTCCGTTGAACACTTCAGAAGGGAGGGCATTTTGAGCAGGGCTTTGATGAACTATCTTGCACTTCTCGGATGGAGAGTGGAAGGAGACGAAATCTTCACTATAGAAGAGAAACTTCAGTCATTCGATCCAAAAGATATTTCGAACAAGGGAGTCATATTCGATTACCAGAAACTCGAATGGGTGAACGGAAAGCACATGAGAAGAATCGATCTCGAAGACCTGAAGAGGGAATTCATCGAATGGGCAAAGTACGCGGGAAAAGAAATCCCCTCCGTGGATGAAAGATACTTCTCAGAGACCCTCCGTATATGTCGGGAAAAGGTGAACACACTCTCTCAGCTGTACGACATCATGTATCCGTTCATGAACGACGATTACGAATACGAAAAGGACTATGTAGAGAAATTTTTGAAGAGAGAAGAAGCCGAAAGGGTGCTTGAAGAAGCAAAAAAAGCCTTCAAAGATCTCAACAGCTGGAATATGGAAGAGATAGAAAAAACATTGAGAGATCTTTCTGAAAAGGGTCTGGCATCGAAAAAGGTGGTCTTTCAGCTCATAAGAGGAGCGGTTACTGGAAAGCTGGTAACACCCGGTCTTTTTGAAACCATAGAGGTGCTGGGAAAAGAAAGAACACTGAAAAGACTTGAAAGAActttacagttcttaaagaaaacctaaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGgcTTCATCACCGGGGCTTTTTTTGATATATTAGAAGTCGGACCAAAAAAAATCAGGAGGTGCTTCGAATTGGTCAGAGTCAGGTTCGCCCCGAGTCCAACAGGCCATCTCCACGTAGGTGGAGCCAGAACCGCTCTGTTCAACTGGATGTTCGCAAGAAAAGAGGGTGGAAAGTTTATCCTTCGAATAGAAGACACCGATACTGAGAGAAGCTCAAGAGAATACGAACAGCAGATCCTGGAATCTTTGAGGTGGTGCGGTCTCGATTGGGACGAAGGGCCTGACATAGGTGGAGATTTCGGACCCTATCGACAGAGCGAAAGACTCGAGATCTACCGAGAATACGCTGAGAAACTGGTGGAAGATAAGAGGGCTTATTACGTGGTTTACGATAAGGAAGATCCCTCTAAAGAGCTCTTCACAACTTACGAGTATCCCCACGAATACAAGGAGAAAGGGCATCCCGTTACCATAAAATTCAAAGTTCTTCCCGGAAAGACTTCCTTCGAGGATCTTCTGAAAGGCTACATGGAATTCGATAACTCCACCTTAGAAGATTTCATCATCATGAAGTCGAACGGATTTCCCACCTATAATTTTGCGGTCGTGGTGGACGATCATCTCATGAGAATCTCCCATGTGTTTCGCGGTGAAGATCACCTTTCTAACACCCCAAAACAGCTCATGATATACGAGGCTTTCGGATGGGAAGCACCCGTCTTCATGCACATTCCACTCATCCTTGGATCGGACAGAACGCCTTTGAGTAAAAGACATGGCGCCACTTCCGTTGAACACTTCAGAAGGGAGGGCATTTTGAGCAGGGCTTTGATGAACTATCTTGCACTTCTCGGATGGAGAGTGGAAGGAGACGAAATCTTCACTATAGAAGAGAAACTTCAGTCATTCGATCCAAAAGATATTTCGAACAAGGGAGTCATATTCGATTACCAGAAACTCGAATGGGTGAACGGAAAGCACATGAGAAGAATCGATCTCGAAGACCTGAAGAGGGAATTCATCGAATGGGCAAAGTACGCGGGAAAAGAAATCCCCTCCGTGGATGAAAGATACTTCTCAGAGACCCTCCGTATATGTCGGGAAAAGGTGAACACACTCTCTCAGCTGTACGACATCATGTATCCGTTCATGAACGACGATTACGAATACGAAAAGGACTATGTAGAGAAATTTTTGAAGAGAGAAGAAGCCGAAAGGGTGCTTGAAGAAGCAAAAAAAGCCTTCAAAGATCTCAACAGCTGGAATATGGAAGAGATAGAAAAAACATTGAGAGATCTTTCTGAAAAGGGTCTGGCATCGAAAAAGGTGGTCTTTCAGCTCATAAGAGGAGCGGTTACTGGAAAGCTGGTAACACCCGGTCTTTTTGAAACCATAGAGGTGCTGGGAAAAGAAAGAACACTGAAAAGACTTGAAAGAActttacagttcttaaagaaaacctaaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	1485	1469	insert ala at start	glutamic acid	4, 1.5
6	pBad myc/hisA		TM-F	phenylalanine	846415..847392,847408..849774	ATGGAGATAGAAGCCGTCGAAAAGGAAGCCATCGAGAAATTATCGAAGATATCGAACGTTCAGGAGCTGGAAAGTTTCAGGATAGAGTTTCTCGGAAAGAAAGGAAAAATCACTGGCTTGATGAAAAACCTCAAGAATCTCCCACCTGAAGAAAGGCCTGCCTACGGTAAAAGAGTGAACGAACTCAGAGAGAAGATAGAAAAACTCTTCGAAGAAAAGAGACAGCAAATCCAAAGGATACTCGAACAGGAAAAGATGGAAAAAATGAGAATAGACGTCACAGTACCGGGGGCAAGGAGGAAACTTGGACATTCTCACCCTGTTCTGAAGGTTATGGAAGAGATAGAGAGAATCTTTGTATCGATGGGATTCGATGTGGTGGAAGGGCCGGAGATCGAAACAACCTGGCACAACTTCGACGCACTGAACACACCGGAATGGCATCCAGCCAGGGACGAACACGATTCTTTCTACATAACGGATGATCTCCTTCTCAGAACTCACACTTCTCCCGTTCAGATCAGAACAATGCTCGAGAGAAAACCTCCCATCGCTATCATATCACCCGGAAAAGTTTACAGAAGAGACTACGATGCGACGCACCTTCCCATGTTTCACCAGGTGGAAGGGTTGCACGTAGATAGAGATCTCAGCGTGGCTCATTTGAAATTCACCCTCGAAGAGTTCGCTCGAAGAATGTTCGGCGAGGGTGCGAAGGTGCGTTTGAGGCCGAGCTTTTTCCCGTTCACGGAACCGAGTTTTGAGGTGGATGTCTATCTTTCCGGGTACGGATGGTTGGAGATTCTGGGAGCAGGCATGGTCGATCCGAACGTCTTCTTGAATGTAGGATACGATCCGGAAGAATGGACGGGTTACGCTTTTGGAATGGGAGTGGAAAGAATCGCCATGCTGAAGTACGGCATAGCAGACATAAGAGAGTTCGTCAGAAACGACGTGAGATTTCTCAGTAGCTACTGAGTGAGAGTTCCCGAGTCTTGGCTCAGAGAATTCGTAGATCTCGACTGGGATATCGAGCAAATAGCGGAAAGACTCACTTTTTCTGGCACGAGTGTGGAGGATATTCTGAGACCTTTCAACGTTTCCGGAGAGATAATCACAGCGAGGGTGATAGAAAGGTTCGATCATCCGGCTTCAGAAAAGCTCATCGTGTGCAAGGTGGACACCGGAAAGAGAATCTACACGGTCATCACAGCCGATAAAACCGTGAACGAAGGAGACTACGTCATACTGGCTCTCGAAGGAGCTACCTTGAACAACGGTCTCAAGATAGAGCCTCGTGAGTTCAAAGGGGTTATCTCGGAGGGTATGCTTTGCTCACTTGAAGAATTGGGACTTGAGGAAAAATCCGACAGAGTTTATCGTTTCCCTGATCCCGTGGAGCTCGGTGTGAACGTGGTAGAGGAATATGGACTCAACGAAAGAGTCCTGGACATAGAGATAACTCCAAACAGGCCTGATTGTCTTTCGATTATTGGTGTTGCGAGAGAGCTTTCTGCCCTCAGCGGAAGGCCTTTGAATAAGCCTCAACCCGATGTTTCTTTTGTGGATGAAGATGTTCAGTTCGATGTGGAGATAGAAGACGTGGAGGGTTGTCCCAGATACTCTGCTCGAATAATGAAAGGTGTTACCGTGAAAGATTCACCGCTCTGGATGAAGGCGAGGCTCGTAGCGGCAGGGGTTCGTTCTCTGAACAACGTGGTCGACGCCACGAACTACGTGATGATCGAGCTGGGACATCCCGTTCATGCGTTCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCAGCCAAAGCGCTTGGAATCAGCTCTGAGTCTTCTTACAGGTTCGAACGTGGTGTGGATCCAAACGATGTGGAGCTCGTATCTTTGAGGCTCGCAGAATTGATTCAAAAGCTGGCCGGCGGTTATGTTCTCAGGAAATTCTGGGATGTTTATCCTCGAAAGATAGAGCCCAAAAAGGTGATGCTCAGAAAAGCCAGGATCGAAAAGATACTGGGAACGAAAGTTGAAGAGCCGGGTGATATTCTCAGACGACTTGAATTCCAGGTGGAAGACAGAGGAGACAGTTACGAAGTCCTTGTTCCCACTTTCAGGCCGGACGTTGAAAGGGAGATAGACCTCATAGAAGAGATAGGAAGGATTTACGGGTACGAGAAAGTCGAGTCGAAAGTGATAAGCGTTCCCGCAGTAAACCGCGGCTGGGGCGAGAAACAGCTCTTCAGAAGGGAAATTTCTCAATTCATGAAAGGAATGGGATTCGATGAAGTGGTGACCTTCTCCTTTGTGGACTCCCAGAAGGTTAAAAAGTGGCCTCTGGTGGACAGAGAACCCATAGCTCTCTCCAACCCAATCGCTTCAGATATGGACGTGATGAGGACGTCACAGTTTTACAGCCTGATACAAGTGCTCGCTGAAAATTTCAAGAGGCAGAACAGAGATCTGAAGCTCTTCGAGATTGGAAAGATTTACTTCAAGGAGAATGGAAACTTCAGGGAGATAGAGACACTCTCTGCCATGAGCTGTGGACTGGAAAATCCGGGAGACTACACCGATAAAAGAAGCGTGTCCTTCTACACGATCAAGGGAGTTCTCGATGAGCTCTTCTTCAGGCTCGGAGTGAACGTCGTATACAGAGCCGCGGAGATACCTGGATTGTTCCCAACCAGATCCGCTCGTATCTACGTGGAAAACAGGGAGATCGGTTTCATTGGAATGGTCGATCCAAAGCTCCTCGATGAGTACGATGTGAAGGAGGATACTTACTTCTTCGAGATAGATATGGAACTTCTGAGAAAATACGCATCGAAGAGACCCGCTTACAGGCCCACACCGAGATTTCCCGCTGTGAGAAGAGATATCTCCTTCCTGCTCCCGAAAGGATTTGAGTCTGTGAAGATCATCGAACTTTTCAAAAAGAGCGGAGGAGATCTGGTGGAGGAAGTGGGAGTGTTCGATATCTATGAAGGTAAAGGGATCCCGGAAAATATGGTGAGCGTTACCTTATATGTGGTGTTCAGACATCCAGAAAGAACCCTGACCGATGAAGAGGTGAATAAAATCTTCGAAGAGATGGTGCAGAAAGCAGAACGAGAATTTGGAATAAGAAGGAGATTCTGA	3345	ccaaaCCATGGagatagaagccgtcgaa	tttggAAGCTTtcagaatctccttcttattcca	ccaaaCCATGGAGATAGAAGCCGTCGAAAAGGAAGCCATCGAGAAATTATCGAAGATATCGAACGTTCAGGAGCTGGAAAGTTTCAGGATAGAGTTTCTCGGAAAGAAAGGAAAAATCACTGGCTTGATGAAAAACCTCAAGAATCTCCCACCTGAAGAAAGGCCTGCCTACGGTAAAAGAGTGAACGAACTCAGAGAGAAGATAGAAAAACTCTTCGAAGAAAAGAGACAGCAAATCCAAAGGATACTCGAACAGGAAAAGATGGAAAAAATGAGAATAGACGTCACAGTACCGGGGGCAAGGAGGAAACTTGGACATTCTCACCCTGTTCTGAAGGTTATGGAAGAGATAGAGAGAATCTTTGTATCGATGGGATTCGATGTGGTGGAAGGGCCGGAGATCGAAACAACCTGGCACAACTTCGACGCACTGAACACACCGGAATGGCATCCAGCCAGGGACGAACACGATTCTTTCTACATAACGGATGATCTCCTTCTCAGAACTCACACTTCTCCCGTTCAGATCAGAACAATGCTCGAGAGAAAACCTCCCATCGCTATCATATCACCCGGAAAAGTTTACAGAAGAGACTACGATGCGACGCACCTTCCCATGTTTCACCAGGTGGAAGGGTTGCACGTAGATAGAGATCTCAGCGTGGCTCATTTGAAATTCACCCTCGAAGAGTTCGCTCGAAGAATGTTCGGCGAGGGTGCGAAGGTGCGTTTGAGGCCGAGCTTTTTCCCGTTCACGGAACCGAGTTTTGAGGTGGATGTCTATCTTTCCGGGTACGGATGGTTGGAGATTCTGGGAGCAGGCATGGTCGATCCGAACGTCTTCTTGAATGTAGGATACGATCCGGAAGAATGGACGGGTTACGCTTTTGGAATGGGAGTGGAAAGAATCGCCATGCTGAAGTACGGCATAGCAGACATAAGAGAGTTCGTCAGAAACGACGTGAGATTTCTCAGTAGCTACTGAGTGAGAGTTCCCGAGTCTTGGCTCAGAGAATTCGTAGATCTCGACTGGGATATCGAGCAAATAGCGGAAAGACTCACTTTTTCTGGCACGAGTGTGGAGGATATTCTGAGACCTTTCAACGTTTCCGGAGAGATAATCACAGCGAGGGTGATAGAAAGGTTCGATCATCCGGCTTCAGAAAAGCTCATCGTGTGCAAGGTGGACACCGGAAAGAGAATCTACACGGTCATCACAGCCGATAAAACCGTGAACGAAGGAGACTACGTCATACTGGCTCTCGAAGGAGCTACCTTGAACAACGGTCTCAAGATAGAGCCTCGTGAGTTCAAAGGGGTTATCTCGGAGGGTATGCTTTGCTCACTTGAAGAATTGGGACTTGAGGAAAAATCCGACAGAGTTTATCGTTTCCCTGATCCCGTGGAGCTCGGTGTGAACGTGGTAGAGGAATATGGACTCAACGAAAGAGTCCTGGACATAGAGATAACTCCAAACAGGCCTGATTGTCTTTCGATTATTGGTGTTGCGAGAGAGCTTTCTGCCCTCAGCGGAAGGCCTTTGAATAAGCCTCAACCCGATGTTTCTTTTGTGGATGAAGATGTTCAGTTCGATGTGGAGATAGAAGACGTGGAGGGTTGTCCCAGATACTCTGCTCGAATAATGAAAGGTGTTACCGTGAAAGATTCACCGCTCTGGATGAAGGCGAGGCTCGTAGCGGCAGGGGTTCGTTCTCTGAACAACGTGGTCGACGCCACGAACTACGTGATGATCGAGCTGGGACATCCCGTTCATGCGTTCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCAGCCAAAGCGCTTGGAATCAGCTCTGAGTCTTCTTACAGGTTCGAACGTGGTGTGGATCCAAACGATGTGGAGCTCGTATCTTTGAGGCTCGCAGAATTGATTCAAAAGCTGGCCGGCGGTTATGTTCTCAGGAAATTCTGGGATGTTTATCCTCGAAAGATAGAGCCCAAAAAGGTGATGCTCAGAAAAGCCAGGATCGAAAAGATACTGGGAACGAAAGTTGAAGAGCCGGGTGATATTCTCAGACGACTTGAATTCCAGGTGGAAGACAGAGGAGACAGTTACGAAGTCCTTGTTCCCACTTTCAGGCCGGACGTTGAAAGGGAGATAGACCTCATAGAAGAGATAGGAAGGATTTACGGGTACGAGAAAGTCGAGTCGAAAGTGATAAGCGTTCCCGCAGTAAACCGCGGCTGGGGCGAGAAACAGCTCTTCAGAAGGGAAATTTCTCAATTCATGAAAGGAATGGGATTCGATGAAGTGGTGACCTTCTCCTTTGTGGACTCCCAGAAGGTTAAAAAGTGGCCTCTGGTGGACAGAGAACCCATAGCTCTCTCCAACCCAATCGCTTCAGATATGGACGTGATGAGGACGTCACAGTTTTACAGCCTGATACAAGTGCTCGCTGAAAATTTCAAGAGGCAGAACAGAGATCTGAAGCTCTTCGAGATTGGAAAGATTTACTTCAAGGAGAATGGAAACTTCAGGGAGATAGAGACACTCTCTGCCATGAGCTGTGGACTGGAAAATCCGGGAGACTACACCGATAAAAGAAGCGTGTCCTTCTACACGATCAAGGGAGTTCTCGATGAGCTCTTCTTCAGGCTCGGAGTGAACGTCGTATACAGAGCCGCGGAGATACCTGGATTGTTCCCAACCAGATCCGCTCGTATCTACGTGGAAAACAGGGAGATCGGTTTCATTGGAATGGTCGATCCAAAGCTCCTCGATGAGTACGATGTGAAGGAGGATACTTACTTCTTCGAGATAGATATGGAACTTCTGAGAAAATACGCATCGAAGAGACCCGCTTACAGGCCCACACCGAGATTTCCCGCTGTGAGAAGAGATATCTCCTTCCTGCTCCCGAAAGGATTTGAGTCTGTGAAGATCATCGAACTTTTCAAAAAGAGCGGAGGAGATCTGGTGGAGGAAGTGGGAGTGTTCGATATCTATGAAGGTAAAGGGATCCCGGAAAATATGGTGAGCGTTACCTTATATGTGGTGTTCAGACATCCAGAAAGAACCCTGACCGATGAAGAGGTGAATAAAATCTTCGAAGAGATGGTGCAGAAAGCAGAACGAGAATTtggaataagaaggagattctgaAAGCTTccaaa	CATGGAGATAGAAGCCGTCGAAAAGGAAGCCATCGAGAAATTATCGAAGATATCGAACGTTCAGGAGCTGGAAAGTTTCAGGATAGAGTTTCTCGGAAAGAAAGGAAAAATCACTGGCTTGATGAAAAACCTCAAGAATCTCCCACCTGAAGAAAGGCCTGCCTACGGTAAAAGAGTGAACGAACTCAGAGAGAAGATAGAAAAACTCTTCGAAGAAAAGAGACAGCAAATCCAAAGGATACTCGAACAGGAAAAGATGGAAAAAATGAGAATAGACGTCACAGTACCGGGGGCAAGGAGGAAACTTGGACATTCTCACCCTGTTCTGAAGGTTATGGAAGAGATAGAGAGAATCTTTGTATCGATGGGATTCGATGTGGTGGAAGGGCCGGAGATCGAAACAACCTGGCACAACTTCGACGCACTGAACACACCGGAATGGCATCCAGCCAGGGACGAACACGATTCTTTCTACATAACGGATGATCTCCTTCTCAGAACTCACACTTCTCCCGTTCAGATCAGAACAATGCTCGAGAGAAAACCTCCCATCGCTATCATATCACCCGGAAAAGTTTACAGAAGAGACTACGATGCGACGCACCTTCCCATGTTTCACCAGGTGGAAGGGTTGCACGTAGATAGAGATCTCAGCGTGGCTCATTTGAAATTCACCCTCGAAGAGTTCGCTCGAAGAATGTTCGGCGAGGGTGCGAAGGTGCGTTTGAGGCCGAGCTTTTTCCCGTTCACGGAACCGAGTTTTGAGGTGGATGTCTATCTTTCCGGGTACGGATGGTTGGAGATTCTGGGAGCAGGCATGGTCGATCCGAACGTCTTCTTGAATGTAGGATACGATCCGGAAGAATGGACGGGTTACGCTTTTGGAATGGGAGTGGAAAGAATCGCCATGCTGAAGTACGGCATAGCAGACATAAGAGAGTTCGTCAGAAACGACGTGAGATTTCTCAGTAGCTACTGAGTGAGAGTTCCCGAGTCTTGGCTCAGAGAATTCGTAGATCTCGACTGGGATATCGAGCAAATAGCGGAAAGACTCACTTTTTCTGGCACGAGTGTGGAGGATATTCTGAGACCTTTCAACGTTTCCGGAGAGATAATCACAGCGAGGGTGATAGAAAGGTTCGATCATCCGGCTTCAGAAAAGCTCATCGTGTGCAAGGTGGACACCGGAAAGAGAATCTACACGGTCATCACAGCCGATAAAACCGTGAACGAAGGAGACTACGTCATACTGGCTCTCGAAGGAGCTACCTTGAACAACGGTCTCAAGATAGAGCCTCGTGAGTTCAAAGGGGTTATCTCGGAGGGTATGCTTTGCTCACTTGAAGAATTGGGACTTGAGGAAAAATCCGACAGAGTTTATCGTTTCCCTGATCCCGTGGAGCTCGGTGTGAACGTGGTAGAGGAATATGGACTCAACGAAAGAGTCCTGGACATAGAGATAACTCCAAACAGGCCTGATTGTCTTTCGATTATTGGTGTTGCGAGAGAGCTTTCTGCCCTCAGCGGAAGGCCTTTGAATAAGCCTCAACCCGATGTTTCTTTTGTGGATGAAGATGTTCAGTTCGATGTGGAGATAGAAGACGTGGAGGGTTGTCCCAGATACTCTGCTCGAATAATGAAAGGTGTTACCGTGAAAGATTCACCGCTCTGGATGAAGGCGAGGCTCGTAGCGGCAGGGGTTCGTTCTCTGAACAACGTGGTCGACGCCACGAACTACGTGATGATCGAGCTGGGACATCCCGTTCATGCGTTCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCAGCCAAAGCGCTTGGAATCAGCTCTGAGTCTTCTTACAGGTTCGAACGTGGTGTGGATCCAAACGATGTGGAGCTCGTATCTTTGAGGCTCGCAGAATTGATTCAAAAGCTGGCCGGCGGTTATGTTCTCAGGAAATTCTGGGATGTTTATCCTCGAAAGATAGAGCCCAAAAAGGTGATGCTCAGAAAAGCCAGGATCGAAAAGATACTGGGAACGAAAGTTGAAGAGCCGGGTGATATTCTCAGACGACTTGAATTCCAGGTGGAAGACAGAGGAGACAGTTACGAAGTCCTTGTTCCCACTTTCAGGCCGGACGTTGAAAGGGAGATAGACCTCATAGAAGAGATAGGAAGGATTTACGGGTACGAGAAAGTCGAGTCGAAAGTGATAAGCGTTCCCGCAGTAAACCGCGGCTGGGGCGAGAAACAGCTCTTCAGAAGGGAAATTTCTCAATTCATGAAAGGAATGGGATTCGATGAAGTGGTGACCTTCTCCTTTGTGGACTCCCAGAAGGTTAAAAAGTGGCCTCTGGTGGACAGAGAACCCATAGCTCTCTCCAACCCAATCGCTTCAGATATGGACGTGATGAGGACGTCACAGTTTTACAGCCTGATACAAGTGCTCGCTGAAAATTTCAAGAGGCAGAACAGAGATCTGAAGCTCTTCGAGATTGGAAAGATTTACTTCAAGGAGAATGGAAACTTCAGGGAGATAGAGACACTCTCTGCCATGAGCTGTGGACTGGAAAATCCGGGAGACTACACCGATAAAAGAAGCGTGTCCTTCTACACGATCAAGGGAGTTCTCGATGAGCTCTTCTTCAGGCTCGGAGTGAACGTCGTATACAGAGCCGCGGAGATACCTGGATTGTTCCCAACCAGATCCGCTCGTATCTACGTGGAAAACAGGGAGATCGGTTTCATTGGAATGGTCGATCCAAAGCTCCTCGATGAGTACGATGTGAAGGAGGATACTTACTTCTTCGAGATAGATATGGAACTTCTGAGAAAATACGCATCGAAGAGACCCGCTTACAGGCCCACACCGAGATTTCCCGCTGTGAGAAGAGATATCTCCTTCCTGCTCCCGAAAGGATTTGAGTCTGTGAAGATCATCGAACTTTTCAAAAAGAGCGGAGGAGATCTGGTGGAGGAAGTGGGAGTGTTCGATATCTATGAAGGTAAAGGGATCCCGGAAAATATGGTGAGCGTTACCTTATATGTGGTGTTCAGACATCCAGAAAGAACCCTGACCGATGAAGAGGTGAATAAAATCTTCGAAGAGATGGTGCAGAAAGCAGAACGAGAATTtggaataagaaggagattctgaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGAGATAGAAGCCGTCGAAAAGGAAGCCATCGAGAAATTATCGAAGATATCGAACGTTCAGGAGCTGGAAAGTTTCAGGATAGAGTTTCTCGGAAAGAAAGGAAAAATCACTGGCTTGATGAAAAACCTCAAGAATCTCCCACCTGAAGAAAGGCCTGCCTACGGTAAAAGAGTGAACGAACTCAGAGAGAAGATAGAAAAACTCTTCGAAGAAAAGAGACAGCAAATCCAAAGGATACTCGAACAGGAAAAGATGGAAAAAATGAGAATAGACGTCACAGTACCGGGGGCAAGGAGGAAACTTGGACATTCTCACCCTGTTCTGAAGGTTATGGAAGAGATAGAGAGAATCTTTGTATCGATGGGATTCGATGTGGTGGAAGGGCCGGAGATCGAAACAACCTGGCACAACTTCGACGCACTGAACACACCGGAATGGCATCCAGCCAGGGACGAACACGATTCTTTCTACATAACGGATGATCTCCTTCTCAGAACTCACACTTCTCCCGTTCAGATCAGAACAATGCTCGAGAGAAAACCTCCCATCGCTATCATATCACCCGGAAAAGTTTACAGAAGAGACTACGATGCGACGCACCTTCCCATGTTTCACCAGGTGGAAGGGTTGCACGTAGATAGAGATCTCAGCGTGGCTCATTTGAAATTCACCCTCGAAGAGTTCGCTCGAAGAATGTTCGGCGAGGGTGCGAAGGTGCGTTTGAGGCCGAGCTTTTTCCCGTTCACGGAACCGAGTTTTGAGGTGGATGTCTATCTTTCCGGGTACGGATGGTTGGAGATTCTGGGAGCAGGCATGGTCGATCCGAACGTCTTCTTGAATGTAGGATACGATCCGGAAGAATGGACGGGTTACGCTTTTGGAATGGGAGTGGAAAGAATCGCCATGCTGAAGTACGGCATAGCAGACATAAGAGAGTTCGTCAGAAACGACGTGAGATTTCTCAGTAGCTACTGAGTGAGAGTTCCCGAGTCTTGGCTCAGAGAATTCGTAGATCTCGACTGGGATATCGAGCAAATAGCGGAAAGACTCACTTTTTCTGGCACGAGTGTGGAGGATATTCTGAGACCTTTCAACGTTTCCGGAGAGATAATCACAGCGAGGGTGATAGAAAGGTTCGATCATCCGGCTTCAGAAAAGCTCATCGTGTGCAAGGTGGACACCGGAAAGAGAATCTACACGGTCATCACAGCCGATAAAACCGTGAACGAAGGAGACTACGTCATACTGGCTCTCGAAGGAGCTACCTTGAACAACGGTCTCAAGATAGAGCCTCGTGAGTTCAAAGGGGTTATCTCGGAGGGTATGCTTTGCTCACTTGAAGAATTGGGACTTGAGGAAAAATCCGACAGAGTTTATCGTTTCCCTGATCCCGTGGAGCTCGGTGTGAACGTGGTAGAGGAATATGGACTCAACGAAAGAGTCCTGGACATAGAGATAACTCCAAACAGGCCTGATTGTCTTTCGATTATTGGTGTTGCGAGAGAGCTTTCTGCCCTCAGCGGAAGGCCTTTGAATAAGCCTCAACCCGATGTTTCTTTTGTGGATGAAGATGTTCAGTTCGATGTGGAGATAGAAGACGTGGAGGGTTGTCCCAGATACTCTGCTCGAATAATGAAAGGTGTTACCGTGAAAGATTCACCGCTCTGGATGAAGGCGAGGCTCGTAGCGGCAGGGGTTCGTTCTCTGAACAACGTGGTCGACGCCACGAACTACGTGATGATCGAGCTGGGACATCCCGTTCATGCGTTCGACCTGAACAGGCTCAAAAATAAAAGAATCGTTGTGAAATCTGCAAAGGGTGGAGAAAGAGTCCTGCTACTCGATGAAAAGGAGTACGAACTGAAGGGTGGAGAGGTCTTGATAACAGACGGTGAGAACGTCCTCGCACTTGGCGGAATCATGGGAGGAATGGAATCTGGTGTGTACGATGACACCAGAGATCTGGTGCTGGAAGTGGCTTACTTCGATCCTGTGAGGATAAGAAAGGCAGCCAAAGCGCTTGGAATCAGCTCTGAGTCTTCTTACAGGTTCGAACGTGGTGTGGATCCAAACGATGTGGAGCTCGTATCTTTGAGGCTCGCAGAATTGATTCAAAAGCTGGCCGGCGGTTATGTTCTCAGGAAATTCTGGGATGTTTATCCTCGAAAGATAGAGCCCAAAAAGGTGATGCTCAGAAAAGCCAGGATCGAAAAGATACTGGGAACGAAAGTTGAAGAGCCGGGTGATATTCTCAGACGACTTGAATTCCAGGTGGAAGACAGAGGAGACAGTTACGAAGTCCTTGTTCCCACTTTCAGGCCGGACGTTGAAAGGGAGATAGACCTCATAGAAGAGATAGGAAGGATTTACGGGTACGAGAAAGTCGAGTCGAAAGTGATAAGCGTTCCCGCAGTAAACCGCGGCTGGGGCGAGAAACAGCTCTTCAGAAGGGAAATTTCTCAATTCATGAAAGGAATGGGATTCGATGAAGTGGTGACCTTCTCCTTTGTGGACTCCCAGAAGGTTAAAAAGTGGCCTCTGGTGGACAGAGAACCCATAGCTCTCTCCAACCCAATCGCTTCAGATATGGACGTGATGAGGACGTCACAGTTTTACAGCCTGATACAAGTGCTCGCTGAAAATTTCAAGAGGCAGAACAGAGATCTGAAGCTCTTCGAGATTGGAAAGATTTACTTCAAGGAGAATGGAAACTTCAGGGAGATAGAGACACTCTCTGCCATGAGCTGTGGACTGGAAAATCCGGGAGACTACACCGATAAAAGAAGCGTGTCCTTCTACACGATCAAGGGAGTTCTCGATGAGCTCTTCTTCAGGCTCGGAGTGAACGTCGTATACAGAGCCGCGGAGATACCTGGATTGTTCCCAACCAGATCCGCTCGTATCTACGTGGAAAACAGGGAGATCGGTTTCATTGGAATGGTCGATCCAAAGCTCCTCGATGAGTACGATGTGAAGGAGGATACTTACTTCTTCGAGATAGATATGGAACTTCTGAGAAAATACGCATCGAAGAGACCCGCTTACAGGCCCACACCGAGATTTCCCGCTGTGAGAAGAGATATCTCCTTCCTGCTCCCGAAAGGATTTGAGTCTGTGAAGATCATCGAACTTTTCAAAAAGAGCGGAGGAGATCTGGTGGAGGAAGTGGGAGTGTTCGATATCTATGAAGGTAAAGGGATCCCGGAAAATATGGTGAGCGTTACCTTATATGTGGTGTTCAGACATCCAGAAAGAACCCTGACCGATGAAGAGGTGAATAAAATCTTCGAAGAGATGGTGCAGAAAGCAGAACGAGAATTtggaataagaaggagattctgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	3363	3347	alpha and beta	phenylalanine	4, 3.3
7	pBad myc/hisA		TM-G	glycine	230581..231441,231422..233440	ATGTATCTTCAGGATGTGATAATGAAACTGAACGATTTCTGGGCGTCTAAAGGTTGTCTGCTTGAACAACCTTACGATATGGAAGTCGGTGCCGGAACGTTTCACCCCGCTACGTTCTTTGGTAGCCTGAGAAAGGGTCCTTGGAAAGTGGCCTACGTTCAGCCAAGCAGAAGGCCGACCGATGGTAGATATGGAGAGAATCCCAACAGATTGCAGAGGTACTTCCAGTACCAGGTGATCATAAAACCCTCCCCTGAAAACTCTCAAGAACTGTATCTTGAGTCCCTGGAGTACCTCGGTATAAACCTGAAGGAACACGACATCAGATTCGTGGAGGACAATTGGGAATCTCCCACCCTCGGAGCGTGGGGTGTTGGTTGGGAAGTGTGGTTAGACGGCATGGAGATAACCCAGTTCACATATTTCCAGCAGATTGGTGGAATTTCCCTGAAGGACATTCCTCTCGAGATAACCTACGGGCTGGAAAGGATCGCCATGTATCTTCAGGGAGTCGATAACGTTTACGAGGTACAGTGGAATGAAAACGTGAAATACGGTGATGTGTTCCTTGAAAACGAAAGAGAATTTTCGGTTTTCAACTTCGAAGAAGCCAACGTAGGACTCCTTTTCAGACATTTCGATGAGTACGAGAAGGAGTTTTACAGACTTGTGGAAAAAAATCTTTACCTTCCCGCTTACGATTACATTTTGAAATGTTCTCACACGTTCAATCTTCTCGATGCGCGCGGAGCGATAAGTGTGTCTCAGCGACAAACCTACGTGAAACGTATTCAGGCGATGGCAAGAAAGGCTGCGAGAGTTTTTCTGGAGGTGCAGGCTAATGAGAACAGCCCTGCTTGAATGAGAACAGCCCTGCTTGAGGTGGGACTCGAAGAACTTCCCGCGAGCGAATTCCACAGTATTCTGAAACAGCTCGAGGAAAAATCGGCTGAGCTTCTCAAAGCGTACAGAGTTTCTTCCGGATCTGTTGAAGTCTTCGTTGGAAGCAGACGTTTTGGTGTGATTCTCAAGAATCTTCCGGAGAGACAGGAGGATTTTACCGAAGAGAAGAAAGGTCCACCACTGAACGTTGCTTACGATGAAAACGGAAAACCAACCAGAGCTCTTGAGGGGTTTTTGAGGAACAACAACGCTTCCTTGGAGAACGTGGTTCACAGGGAGGGGTACGTCTATTTATCGCGAGTTGTTGAAGGAAAGCCTGTCGAAGAAGTTCTCCCAGATCTGTTCAGAGATCTCGTTTTGGGTTTGAATTTTAGAAAACCCATGAGGTGGGGTTCCGGTGAACACGAATACATCAGACCTGTTCACTGGATTGTGGCGATGGTTGATGGAAGAGTTCTTGACTTAGAAATCTTCGGTCTTAGATCGTCCAGAATCTCTTACGGCAAAAGGTATCACGCTGGATCAATCGAAATTCCCGATTCGGAAAGGTACTACGAATCTCTCAAAAAAGGATTCGTGATTTCTTCCCACCTGGAGAGAAAAAAATTCGTCCTCGAACAGATCGATGAATTTGAAAAAAGAAGTAGTATGAAGATCGAACGCGATGAAGAACTCATCGAAGAGATAGTGGCGATAACGGAGTATCCCAGGATCGTTGTTGGACAGTTCGATCGAAAATATCTCGAACTTCCAGAGGAGATCATAGTAACCGCCGTGAAACATCATCAACGCTCTTTTATAGCGCATAAAGGAACCCTGACGAATACCTTTGTGGCTTTTCAAGACGGTCCACAACCACCGGAGAACGTGGTTAAGGGATACGAGAGAGTCATAAACGCTCGATTGGAAGACGCGCGGTACTACTTCCAGAAGGATCTCGAGACACCGCTGGAAAAGATGAATGAAAAGCTCAAAGAAATCGTTTTTCAGGAGAAACTCGGAACACTCTACGATAAGGTAGAAAGGATAAAGAAAATATCACAGAGACTCTGTGAAGATCTGAAACTTCCAGGGTCGTTCACGCAGAAGGTCCTGGAAGCCGCCTCTATATGCAAAGCAGACATCGCTTCGAAGGTGGTGTACGAGTTCCCGGAGCTCCAGGGTGTTATGGGTAGGATCTATGCCCTGAGGGAGGGAATAAACGAAGAAATAGCAACGGCCATAGAAGATCACTACTCTGAAGAGCCACAGACAGTGATAGGTTCCATTCTTGGAATAGCGGATAGAATCGACACCATCGTGGGAAATTTCGCCATAGGCAACGTTCCCACGAGTTCTAAAGATCCGTATGGCCTGAAAAGTAAAGCTGACACAATCTTCAGGATCATAAGGAAGAACGAGTGGGATATTTCTCTGGAAGAGCTTCTCACTTTCGCTTCTTCACTGGTGGGGTACCGTCTTTCTGAAGAACTGGAAACGTTCTTTGCAGGCAGGTTCTATCAGTTTCTGATCAACGAACTTGGAATATCCTTCGACGTGGCAAGAGCGGTGAATCACCTGTGGAAGAAGCCCCTTCGCGGGATCCTCTCTGCGGAAGCCCTCCAAGAGATATCTGAAAAACCCGAGTTTCAAGACCTGTTCGTTGGATTTGAGCGAGTCCACAACATAACGAAGAATCACGACTCCACCAAATTCGATGGAGCCCTCTTTGAGAAAGAAGAAGAGAAAAAGCTGATGAACAAGTTCTACGAAGTCAAAGAAAAAGTTTTGAAAGCTCTGGAGAGGTTGAACTACCGTGAGGCGCTTCAGTATCTGATCGAATTGAAACCTTACATAGACGAGTACTTCGACAATGTCTTTGTGATGGTGAAGAGGGACGACTTGAGAGTGAACAGGTTGAGCTTTCTGAAAAACATCGACGAACTCTTCATGATGGTAGGAGACATGACTTACTTAGTCAAGAGATCTCAGGTATAG	2880	ccaaaCCATGGcgtatcttcaggatgtgataatgaaac	tttggAAGCTTctatacctgagatctcttgac	ccaaaCCATGGcgTATCTTCAGGATGTGATAATGAAACTGAACGATTTCTGGGCGTCTAAAGGTTGTCTGCTTGAACAACCTTACGATATGGAAGTCGGTGCCGGAACGTTTCACCCCGCTACGTTCTTTGGTAGCCTGAGAAAGGGTCCTTGGAAAGTGGCCTACGTTCAGCCAAGCAGAAGGCCGACCGATGGTAGATATGGAGAGAATCCCAACAGATTGCAGAGGTACTTCCAGTACCAGGTGATCATAAAACCCTCCCCTGAAAACTCTCAAGAACTGTATCTTGAGTCCCTGGAGTACCTCGGTATAAACCTGAAGGAACACGACATCAGATTCGTGGAGGACAATTGGGAATCTCCCACCCTCGGAGCGTGGGGTGTTGGTTGGGAAGTGTGGTTAGACGGCATGGAGATAACCCAGTTCACATATTTCCAGCAGATTGGTGGAATTTCCCTGAAGGACATTCCTCTCGAGATAACCTACGGGCTGGAAAGGATCGCCATGTATCTTCAGGGAGTCGATAACGTTTACGAGGTACAGTGGAATGAAAACGTGAAATACGGTGATGTGTTCCTTGAAAACGAAAGAGAATTTTCGGTTTTCAACTTCGAAGAAGCCAACGTAGGACTCCTTTTCAGACATTTCGATGAGTACGAGAAGGAGTTTTACAGACTTGTGGAAAAAAATCTTTACCTTCCCGCTTACGATTACATTTTGAAATGTTCTCACACGTTCAATCTTCTCGATGCGCGCGGAGCGATAAGTGTGTCTCAGCGACAAACCTACGTGAAACGTATTCAGGCGATGGCAAGAAAGGCTGCGAGAGTTTTTCTGGAGGTGCAGGCTAATGAGAACAGCCCTGCTTGAATGAGAACAGCCCTGCTTGAGGTGGGACTCGAAGAACTTCCCGCGAGCGAATTCCACAGTATTCTGAAACAGCTCGAGGAAAAATCGGCTGAGCTTCTCAAAGCGTACAGAGTTTCTTCCGGATCTGTTGAAGTCTTCGTTGGAAGCAGACGTTTTGGTGTGATTCTCAAGAATCTTCCGGAGAGACAGGAGGATTTTACCGAAGAGAAGAAAGGTCCACCACTGAACGTTGCTTACGATGAAAACGGAAAACCAACCAGAGCTCTTGAGGGGTTTTTGAGGAACAACAACGCTTCCTTGGAGAACGTGGTTCACAGGGAGGGGTACGTCTATTTATCGCGAGTTGTTGAAGGAAAGCCTGTCGAAGAAGTTCTCCCAGATCTGTTCAGAGATCTCGTTTTGGGTTTGAATTTTAGAAAACCCATGAGGTGGGGTTCCGGTGAACACGAATACATCAGACCTGTTCACTGGATTGTGGCGATGGTTGATGGAAGAGTTCTTGACTTAGAAATCTTCGGTCTTAGATCGTCCAGAATCTCTTACGGCAAAAGGTATCACGCTGGATCAATCGAAATTCCCGATTCGGAAAGGTACTACGAATCTCTCAAAAAAGGATTCGTGATTTCTTCCCACCTGGAGAGAAAAAAATTCGTCCTCGAACAGATCGATGAATTTGAAAAAAGAAGTAGTATGAAGATCGAACGCGATGAAGAACTCATCGAAGAGATAGTGGCGATAACGGAGTATCCCAGGATCGTTGTTGGACAGTTCGATCGAAAATATCTCGAACTTCCAGAGGAGATCATAGTAACCGCCGTGAAACATCATCAACGCTCTTTTATAGCGCATAAAGGAACCCTGACGAATACCTTTGTGGCTTTTCAAGACGGTCCACAACCACCGGAGAACGTGGTTAAGGGATACGAGAGAGTCATAAACGCTCGATTGGAAGACGCGCGGTACTACTTCCAGAAGGATCTCGAGACACCGCTGGAAAAGATGAATGAAAAGCTCAAAGAAATCGTTTTTCAGGAGAAACTCGGAACACTCTACGATAAGGTAGAAAGGATAAAGAAAATATCACAGAGACTCTGTGAAGATCTGAAACTTCCAGGGTCGTTCACGCAGAAGGTCCTGGAAGCCGCCTCTATATGCAAAGCAGACATCGCTTCGAAGGTGGTGTACGAGTTCCCGGAGCTCCAGGGTGTTATGGGTAGGATCTATGCCCTGAGGGAGGGAATAAACGAAGAAATAGCAACGGCCATAGAAGATCACTACTCTGAAGAGCCACAGACAGTGATAGGTTCCATTCTTGGAATAGCGGATAGAATCGACACCATCGTGGGAAATTTCGCCATAGGCAACGTTCCCACGAGTTCTAAAGATCCGTATGGCCTGAAAAGTAAAGCTGACACAATCTTCAGGATCATAAGGAAGAACGAGTGGGATATTTCTCTGGAAGAGCTTCTCACTTTCGCTTCTTCACTGGTGGGGTACCGTCTTTCTGAAGAACTGGAAACGTTCTTTGCAGGCAGGTTCTATCAGTTTCTGATCAACGAACTTGGAATATCCTTCGACGTGGCAAGAGCGGTGAATCACCTGTGGAAGAAGCCCCTTCGCGGGATCCTCTCTGCGGAAGCCCTCCAAGAGATATCTGAAAAACCCGAGTTTCAAGACCTGTTCGTTGGATTTGAGCGAGTCCACAACATAACGAAGAATCACGACTCCACCAAATTCGATGGAGCCCTCTTTGAGAAAGAAGAAGAGAAAAAGCTGATGAACAAGTTCTACGAAGTCAAAGAAAAAGTTTTGAAAGCTCTGGAGAGGTTGAACTACCGTGAGGCGCTTCAGTATCTGATCGAATTGAAACCTTACATAGACGAGTACTTCGACAATGTCTTTGTGATGGTGAAGAGGGACGACTTGAGAGTGAACAGGTTGAGCTTTCTGAAAAACATCGACGAACTCTTCATGATGGTAGGAGACATGACTTACTTAgtcaagagatctcaggtatagAAGCTTccaaa	CATGGcgTATCTTCAGGATGTGATAATGAAACTGAACGATTTCTGGGCGTCTAAAGGTTGTCTGCTTGAACAACCTTACGATATGGAAGTCGGTGCCGGAACGTTTCACCCCGCTACGTTCTTTGGTAGCCTGAGAAAGGGTCCTTGGAAAGTGGCCTACGTTCAGCCAAGCAGAAGGCCGACCGATGGTAGATATGGAGAGAATCCCAACAGATTGCAGAGGTACTTCCAGTACCAGGTGATCATAAAACCCTCCCCTGAAAACTCTCAAGAACTGTATCTTGAGTCCCTGGAGTACCTCGGTATAAACCTGAAGGAACACGACATCAGATTCGTGGAGGACAATTGGGAATCTCCCACCCTCGGAGCGTGGGGTGTTGGTTGGGAAGTGTGGTTAGACGGCATGGAGATAACCCAGTTCACATATTTCCAGCAGATTGGTGGAATTTCCCTGAAGGACATTCCTCTCGAGATAACCTACGGGCTGGAAAGGATCGCCATGTATCTTCAGGGAGTCGATAACGTTTACGAGGTACAGTGGAATGAAAACGTGAAATACGGTGATGTGTTCCTTGAAAACGAAAGAGAATTTTCGGTTTTCAACTTCGAAGAAGCCAACGTAGGACTCCTTTTCAGACATTTCGATGAGTACGAGAAGGAGTTTTACAGACTTGTGGAAAAAAATCTTTACCTTCCCGCTTACGATTACATTTTGAAATGTTCTCACACGTTCAATCTTCTCGATGCGCGCGGAGCGATAAGTGTGTCTCAGCGACAAACCTACGTGAAACGTATTCAGGCGATGGCAAGAAAGGCTGCGAGAGTTTTTCTGGAGGTGCAGGCTAATGAGAACAGCCCTGCTTGAATGAGAACAGCCCTGCTTGAGGTGGGACTCGAAGAACTTCCCGCGAGCGAATTCCACAGTATTCTGAAACAGCTCGAGGAAAAATCGGCTGAGCTTCTCAAAGCGTACAGAGTTTCTTCCGGATCTGTTGAAGTCTTCGTTGGAAGCAGACGTTTTGGTGTGATTCTCAAGAATCTTCCGGAGAGACAGGAGGATTTTACCGAAGAGAAGAAAGGTCCACCACTGAACGTTGCTTACGATGAAAACGGAAAACCAACCAGAGCTCTTGAGGGGTTTTTGAGGAACAACAACGCTTCCTTGGAGAACGTGGTTCACAGGGAGGGGTACGTCTATTTATCGCGAGTTGTTGAAGGAAAGCCTGTCGAAGAAGTTCTCCCAGATCTGTTCAGAGATCTCGTTTTGGGTTTGAATTTTAGAAAACCCATGAGGTGGGGTTCCGGTGAACACGAATACATCAGACCTGTTCACTGGATTGTGGCGATGGTTGATGGAAGAGTTCTTGACTTAGAAATCTTCGGTCTTAGATCGTCCAGAATCTCTTACGGCAAAAGGTATCACGCTGGATCAATCGAAATTCCCGATTCGGAAAGGTACTACGAATCTCTCAAAAAAGGATTCGTGATTTCTTCCCACCTGGAGAGAAAAAAATTCGTCCTCGAACAGATCGATGAATTTGAAAAAAGAAGTAGTATGAAGATCGAACGCGATGAAGAACTCATCGAAGAGATAGTGGCGATAACGGAGTATCCCAGGATCGTTGTTGGACAGTTCGATCGAAAATATCTCGAACTTCCAGAGGAGATCATAGTAACCGCCGTGAAACATCATCAACGCTCTTTTATAGCGCATAAAGGAACCCTGACGAATACCTTTGTGGCTTTTCAAGACGGTCCACAACCACCGGAGAACGTGGTTAAGGGATACGAGAGAGTCATAAACGCTCGATTGGAAGACGCGCGGTACTACTTCCAGAAGGATCTCGAGACACCGCTGGAAAAGATGAATGAAAAGCTCAAAGAAATCGTTTTTCAGGAGAAACTCGGAACACTCTACGATAAGGTAGAAAGGATAAAGAAAATATCACAGAGACTCTGTGAAGATCTGAAACTTCCAGGGTCGTTCACGCAGAAGGTCCTGGAAGCCGCCTCTATATGCAAAGCAGACATCGCTTCGAAGGTGGTGTACGAGTTCCCGGAGCTCCAGGGTGTTATGGGTAGGATCTATGCCCTGAGGGAGGGAATAAACGAAGAAATAGCAACGGCCATAGAAGATCACTACTCTGAAGAGCCACAGACAGTGATAGGTTCCATTCTTGGAATAGCGGATAGAATCGACACCATCGTGGGAAATTTCGCCATAGGCAACGTTCCCACGAGTTCTAAAGATCCGTATGGCCTGAAAAGTAAAGCTGACACAATCTTCAGGATCATAAGGAAGAACGAGTGGGATATTTCTCTGGAAGAGCTTCTCACTTTCGCTTCTTCACTGGTGGGGTACCGTCTTTCTGAAGAACTGGAAACGTTCTTTGCAGGCAGGTTCTATCAGTTTCTGATCAACGAACTTGGAATATCCTTCGACGTGGCAAGAGCGGTGAATCACCTGTGGAAGAAGCCCCTTCGCGGGATCCTCTCTGCGGAAGCCCTCCAAGAGATATCTGAAAAACCCGAGTTTCAAGACCTGTTCGTTGGATTTGAGCGAGTCCACAACATAACGAAGAATCACGACTCCACCAAATTCGATGGAGCCCTCTTTGAGAAAGAAGAAGAGAAAAAGCTGATGAACAAGTTCTACGAAGTCAAAGAAAAAGTTTTGAAAGCTCTGGAGAGGTTGAACTACCGTGAGGCGCTTCAGTATCTGATCGAATTGAAACCTTACATAGACGAGTACTTCGACAATGTCTTTGTGATGGTGAAGAGGGACGACTTGAGAGTGAACAGGTTGAGCTTTCTGAAAAACATCGACGAACTCTTCATGATGGTAGGAGACATGACTTACTTAgtcaagagatctcaggtatagA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGcgTATCTTCAGGATGTGATAATGAAACTGAACGATTTCTGGGCGTCTAAAGGTTGTCTGCTTGAACAACCTTACGATATGGAAGTCGGTGCCGGAACGTTTCACCCCGCTACGTTCTTTGGTAGCCTGAGAAAGGGTCCTTGGAAAGTGGCCTACGTTCAGCCAAGCAGAAGGCCGACCGATGGTAGATATGGAGAGAATCCCAACAGATTGCAGAGGTACTTCCAGTACCAGGTGATCATAAAACCCTCCCCTGAAAACTCTCAAGAACTGTATCTTGAGTCCCTGGAGTACCTCGGTATAAACCTGAAGGAACACGACATCAGATTCGTGGAGGACAATTGGGAATCTCCCACCCTCGGAGCGTGGGGTGTTGGTTGGGAAGTGTGGTTAGACGGCATGGAGATAACCCAGTTCACATATTTCCAGCAGATTGGTGGAATTTCCCTGAAGGACATTCCTCTCGAGATAACCTACGGGCTGGAAAGGATCGCCATGTATCTTCAGGGAGTCGATAACGTTTACGAGGTACAGTGGAATGAAAACGTGAAATACGGTGATGTGTTCCTTGAAAACGAAAGAGAATTTTCGGTTTTCAACTTCGAAGAAGCCAACGTAGGACTCCTTTTCAGACATTTCGATGAGTACGAGAAGGAGTTTTACAGACTTGTGGAAAAAAATCTTTACCTTCCCGCTTACGATTACATTTTGAAATGTTCTCACACGTTCAATCTTCTCGATGCGCGCGGAGCGATAAGTGTGTCTCAGCGACAAACCTACGTGAAACGTATTCAGGCGATGGCAAGAAAGGCTGCGAGAGTTTTTCTGGAGGTGCAGGCTAATGAGAACAGCCCTGCTTGAATGAGAACAGCCCTGCTTGAGGTGGGACTCGAAGAACTTCCCGCGAGCGAATTCCACAGTATTCTGAAACAGCTCGAGGAAAAATCGGCTGAGCTTCTCAAAGCGTACAGAGTTTCTTCCGGATCTGTTGAAGTCTTCGTTGGAAGCAGACGTTTTGGTGTGATTCTCAAGAATCTTCCGGAGAGACAGGAGGATTTTACCGAAGAGAAGAAAGGTCCACCACTGAACGTTGCTTACGATGAAAACGGAAAACCAACCAGAGCTCTTGAGGGGTTTTTGAGGAACAACAACGCTTCCTTGGAGAACGTGGTTCACAGGGAGGGGTACGTCTATTTATCGCGAGTTGTTGAAGGAAAGCCTGTCGAAGAAGTTCTCCCAGATCTGTTCAGAGATCTCGTTTTGGGTTTGAATTTTAGAAAACCCATGAGGTGGGGTTCCGGTGAACACGAATACATCAGACCTGTTCACTGGATTGTGGCGATGGTTGATGGAAGAGTTCTTGACTTAGAAATCTTCGGTCTTAGATCGTCCAGAATCTCTTACGGCAAAAGGTATCACGCTGGATCAATCGAAATTCCCGATTCGGAAAGGTACTACGAATCTCTCAAAAAAGGATTCGTGATTTCTTCCCACCTGGAGAGAAAAAAATTCGTCCTCGAACAGATCGATGAATTTGAAAAAAGAAGTAGTATGAAGATCGAACGCGATGAAGAACTCATCGAAGAGATAGTGGCGATAACGGAGTATCCCAGGATCGTTGTTGGACAGTTCGATCGAAAATATCTCGAACTTCCAGAGGAGATCATAGTAACCGCCGTGAAACATCATCAACGCTCTTTTATAGCGCATAAAGGAACCCTGACGAATACCTTTGTGGCTTTTCAAGACGGTCCACAACCACCGGAGAACGTGGTTAAGGGATACGAGAGAGTCATAAACGCTCGATTGGAAGACGCGCGGTACTACTTCCAGAAGGATCTCGAGACACCGCTGGAAAAGATGAATGAAAAGCTCAAAGAAATCGTTTTTCAGGAGAAACTCGGAACACTCTACGATAAGGTAGAAAGGATAAAGAAAATATCACAGAGACTCTGTGAAGATCTGAAACTTCCAGGGTCGTTCACGCAGAAGGTCCTGGAAGCCGCCTCTATATGCAAAGCAGACATCGCTTCGAAGGTGGTGTACGAGTTCCCGGAGCTCCAGGGTGTTATGGGTAGGATCTATGCCCTGAGGGAGGGAATAAACGAAGAAATAGCAACGGCCATAGAAGATCACTACTCTGAAGAGCCACAGACAGTGATAGGTTCCATTCTTGGAATAGCGGATAGAATCGACACCATCGTGGGAAATTTCGCCATAGGCAACGTTCCCACGAGTTCTAAAGATCCGTATGGCCTGAAAAGTAAAGCTGACACAATCTTCAGGATCATAAGGAAGAACGAGTGGGATATTTCTCTGGAAGAGCTTCTCACTTTCGCTTCTTCACTGGTGGGGTACCGTCTTTCTGAAGAACTGGAAACGTTCTTTGCAGGCAGGTTCTATCAGTTTCTGATCAACGAACTTGGAATATCCTTCGACGTGGCAAGAGCGGTGAATCACCTGTGGAAGAAGCCCCTTCGCGGGATCCTCTCTGCGGAAGCCCTCCAAGAGATATCTGAAAAACCCGAGTTTCAAGACCTGTTCGTTGGATTTGAGCGAGTCCACAACATAACGAAGAATCACGACTCCACCAAATTCGATGGAGCCCTCTTTGAGAAAGAAGAAGAGAAAAAGCTGATGAACAAGTTCTACGAAGTCAAAGAAAAAGTTTTGAAAGCTCTGGAGAGGTTGAACTACCGTGAGGCGCTTCAGTATCTGATCGAATTGAAACCTTACATAGACGAGTACTTCGACAATGTCTTTGTGATGGTGAAGAGGGACGACTTGAGAGTGAACAGGTTGAGCTTTCTGAAAAACATCGACGAACTCTTCATGATGGTAGGAGACATGACTTACTTAgtcaagagatctcaggtatagAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	2901	2885		glycine	4, 2.9
8	pBad myc/hisA		TM-H	histidine	1106385..1107647	ttgaaatacagaaggataaagggaacaaacgatatcttcggtgaagagatatggtactggaggtacgtcgaggaaaccttcagaaatgtgtgcgaaagcgcgggaatagaggagatcagaactcccatattcgaacagacggaacttttcgttagaagtgtgggagaagaatcagacatcgttcagaaagagatgtacaccttccaggacaaagcgggaaggagcatcactctgagaccagaaggtaccgcgcccgtcgtcagggcttttctggagaattctctgatagatagggggtttcaacagagatactactacataggtcccatgttccgatacgaaaaaccacaatcggggagattgagacagtttcaccaggtgggtttcgagatcatcggccccgaatctccaaaagcggatttcgaggtgatcatgctggttgataccttcttgagaaggctcggactcacgaaatacaagattcacctgaattccataggctgtccggtgtgcaggaaaaactatcgcgaagccctcaaggagtattacggtcgaatcctggacaatctctgtgacgactgcaaaagacgttacgagacgaacattttgagactgctcgattgcaaggtggaccacgaatactctctgaatgcaccaaaaagcgtcgactatctctgtgattcctgcagggcacactacaagaagttgaaagagtacctcaacactttcgagatcgaatacgtggaagatcacaccctggtccgcgggctcgactactacaccagaacggtttttgaggtgaggcacgaaggtcttggagcccagagcgccatcgcgggtggtggaaggtacgatggcctctttgcggagcttgggggctcttccgtacccgcccttggttttgcaggtggtatagagagaattatactcgctttaaaagcagagggaatagaaatcccgatgaaaaatgttcatcttgtttacatcgcaactcttggtgaaaaagcctttatggatggtgttcggcttgcgggtgaattgagaaagaaaggcctgagtgtggatgtggacatcatggatagaaagctctccggtcagcttaagcacgccagcagaatgggatcgaggtatgcggtcattattggtgatgaagaactggaaaaaggaatcgtcattctccgcgacctcgagacgggagaccaggttgaaatcgatcgggacttcgccgctgactacatcgctgaaagggtttcaaaagattga	1263	ccaaaCCATGGcgaaatacagaaggataaagggaaca	tttggAAGCTTtcaatcttttgaaaccctttcagcg	ccaaaCCATGGcgaaatacagaaggataaagggaacaaacgatatcttcggtgaagagatatggtactggaggtacgtcgaggaaaccttcagaaatgtgtgcgaaagcgcgggaatagaggagatcagaactcccatattcgaacagacggaacttttcgttagaagtgtgggagaagaatcagacatcgttcagaaagagatgtacaccttccaggacaaagcgggaaggagcatcactctgagaccagaaggtaccgcgcccgtcgtcagggcttttctggagaattctctgatagatagggggtttcaacagagatactactacataggtcccatgttccgatacgaaaaaccacaatcggggagattgagacagtttcaccaggtgggtttcgagatcatcggccccgaatctccaaaagcggatttcgaggtgatcatgctggttgataccttcttgagaaggctcggactcacgaaatacaagattcacctgaattccataggctgtccggtgtgcaggaaaaactatcgcgaagccctcaaggagtattacggtcgaatcctggacaatctctgtgacgactgcaaaagacgttacgagacgaacattttgagactgctcgattgcaaggtggaccacgaatactctctgaatgcaccaaaaagcgtcgactatctctgtgattcctgcagggcacactacaagaagttgaaagagtacctcaacactttcgagatcgaatacgtggaagatcacaccctggtccgcgggctcgactactacaccagaacggtttttgaggtgaggcacgaaggtcttggagcccagagcgccatcgcgggtggtggaaggtacgatggcctctttgcggagcttgggggctcttccgtacccgcccttggttttgcaggtggtatagagagaattatactcgctttaaaagcagagggaatagaaatcccgatgaaaaatgttcatcttgtttacatcgcaactcttggtgaaaaagcctttatggatggtgttcggcttgcgggtgaattgagaaagaaaggcctgagtgtggatgtggacatcatggatagaaagctctccggtcagcttaagcacgccagcagaatgggatcgaggtatgcggtcattattggtgatgaagaactggaaaaaggaatcgtcattctccgcgacctcgagacgggagaccaggttgaaatcgatcgggacttcgccgctgactacatcgctgaaagggtttcaaaagattgaAAGCTTccaaa	CATGGcgaaatacagaaggataaagggaacaaacgatatcttcggtgaagagatatggtactggaggtacgtcgaggaaaccttcagaaatgtgtgcgaaagcgcgggaatagaggagatcagaactcccatattcgaacagacggaacttttcgttagaagtgtgggagaagaatcagacatcgttcagaaagagatgtacaccttccaggacaaagcgggaaggagcatcactctgagaccagaaggtaccgcgcccgtcgtcagggcttttctggagaattctctgatagatagggggtttcaacagagatactactacataggtcccatgttccgatacgaaaaaccacaatcggggagattgagacagtttcaccaggtgggtttcgagatcatcggccccgaatctccaaaagcggatttcgaggtgatcatgctggttgataccttcttgagaaggctcggactcacgaaatacaagattcacctgaattccataggctgtccggtgtgcaggaaaaactatcgcgaagccctcaaggagtattacggtcgaatcctggacaatctctgtgacgactgcaaaagacgttacgagacgaacattttgagactgctcgattgcaaggtggaccacgaatactctctgaatgcaccaaaaagcgtcgactatctctgtgattcctgcagggcacactacaagaagttgaaagagtacctcaacactttcgagatcgaatacgtggaagatcacaccctggtccgcgggctcgactactacaccagaacggtttttgaggtgaggcacgaaggtcttggagcccagagcgccatcgcgggtggtggaaggtacgatggcctctttgcggagcttgggggctcttccgtacccgcccttggttttgcaggtggtatagagagaattatactcgctttaaaagcagagggaatagaaatcccgatgaaaaatgttcatcttgtttacatcgcaactcttggtgaaaaagcctttatggatggtgttcggcttgcgggtgaattgagaaagaaaggcctgagtgtggatgtggacatcatggatagaaagctctccggtcagcttaagcacgccagcagaatgggatcgaggtatgcggtcattattggtgatgaagaactggaaaaaggaatcgtcattctccgcgacctcgagacgggagaccaggttgaaatcgatcgggacttcgccgctgactacatcgctgaaagggtttcaaaagattgaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGcgaaatacagaaggataaagggaacaaacgatatcttcggtgaagagatatggtactggaggtacgtcgaggaaaccttcagaaatgtgtgcgaaagcgcgggaatagaggagatcagaactcccatattcgaacagacggaacttttcgttagaagtgtgggagaagaatcagacatcgttcagaaagagatgtacaccttccaggacaaagcgggaaggagcatcactctgagaccagaaggtaccgcgcccgtcgtcagggcttttctggagaattctctgatagatagggggtttcaacagagatactactacataggtcccatgttccgatacgaaaaaccacaatcggggagattgagacagtttcaccaggtgggtttcgagatcatcggccccgaatctccaaaagcggatttcgaggtgatcatgctggttgataccttcttgagaaggctcggactcacgaaatacaagattcacctgaattccataggctgtccggtgtgcaggaaaaactatcgcgaagccctcaaggagtattacggtcgaatcctggacaatctctgtgacgactgcaaaagacgttacgagacgaacattttgagactgctcgattgcaaggtggaccacgaatactctctgaatgcaccaaaaagcgtcgactatctctgtgattcctgcagggcacactacaagaagttgaaagagtacctcaacactttcgagatcgaatacgtggaagatcacaccctggtccgcgggctcgactactacaccagaacggtttttgaggtgaggcacgaaggtcttggagcccagagcgccatcgcgggtggtggaaggtacgatggcctctttgcggagcttgggggctcttccgtacccgcccttggttttgcaggtggtatagagagaattatactcgctttaaaagcagagggaatagaaatcccgatgaaaaatgttcatcttgtttacatcgcaactcttggtgaaaaagcctttatggatggtgttcggcttgcgggtgaattgagaaagaaaggcctgagtgtggatgtggacatcatggatagaaagctctccggtcagcttaagcacgccagcagaatgggatcgaggtatgcggtcattattggtgatgaagaactggaaaaaggaatcgtcattctccgcgacctcgagacgggagaccaggttgaaatcgatcgggacttcgccgctgactacatcgctgaaagggtttcaaaagattgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	1284	1268	insert ala at start	histidine	4, 1.3
9	pBad myc/hisA		TM-I	isoleucine	1379227..1381986	atggaatacaaaaacacactgaacttgccaaaaacttcatttccaatgaaggcaaacttggtgaacaaagaaaaagtttttctcgaagaatgggaaaagatggacctttacaactacgtactcgaacagagaaaaggaaaacccctttttgtcctgcacgatggacccccttacgcaaacggtcacatccacattggaacggccctgaacaagattctgaaggacatcgtcgtcaaatacaaaacgatgagaggatacagggcaccgtacgttcctggctgggacacccacggtcttcccatagaacacagggtgtctcaggaactgggtgaaaagataaaagaaatgtcacccgcagagatcagaaagaagtgtgaggaattcgctctcagattcgttgatatacagagagaagaatttaaaagactcggcgttcggggagactgggaaaatccatacatcacgttgaagccagactacgaggtgaaaatactggatgttttcaaaacccttgtagaacagggaaacgtttacagatctttgaaacccatctactggtgtccgagatgcagaactgccctcgcggaagcggaaatcgaataccacgatcataaatctccttccatatacgtgaagttcagatccaaagaagatccgaatttcttcatcgtgatatggaccacaacaccctggacactgccggcaaacgtcggtatcgctctgcaccccgattacgaatactcggtggtgaaagtcggagaagagaaatgggtcatcgcaacggatcttctcgatgctttttcgaaggagactggaatcgactgttccaatgtcgtcgagaaaataaaaggaaaagatctggaaggaaaagagttcgttcatcccatcttcgatgacaggacctccagagtcattctggccgattacgtatctctcgaaacaggaacaggatgtgttcacatagcccccggtcacggtgaagaagactacatctacggtcatgtgcaatacggtcttcccatcgtctctcccgttgatgaggagggacgtttcacggaagaagcgggaaaatacaagggaatgttcatagaagatgccaacgaggtgatcatagaggatctgaagagaaagggtattctcgttcacgcttcaagtatcacacactcctacccgcactgctggcgctgtaaaggacctgtgatcttcagagcaactgagcagtggttcatttctgtggatcacaacaacctcaggcagaaagtgctggaagaaatagacaaggtgaagtggataccggaatggggccggaacagaatcagatccatggtggaggaaaggcccgactggtgtatctcaagacagcgtgtctggggaactcccataccagcggtgaaatgtaaagaatgcggagaagtggtactcgatccgaaggtgatagaacacttcatgaaaatcgttgaaaaagaaggtacaaacgcctggtttgaaaaagaggtggaagaactcatccccgaagattttgtgtgtccaaaatgcggcaagcgatctttcgaaaaaatgctggatacactcgacgtatggatagattctggtgcctccttcgaatacataaccacgaagagagaagatcacccgttccccctcgacatgtaccttgaaggtagcgatcagcacaggggatggttccattcttccatcttccttgccgtcgcaaagagggggtccgcaccttacaaagaagtgctaacacacggattcataaaggacgaacagggaagaaagatgagcaagtccctcggaaacgtagtggatcccatggaagtggtggaaaaatacggtgccgagattctgagactgtggcttgcgagcagcgactacttcaacgacataaagatatccatgaggatcgtcgaacaacaaacggaagtttacaggaagataagaaacacgttccgattcctccttggaaatctagaggactttgatcctgaacttgacagagttcctcatgaaaaactcttaacgatcgacagatgggctcttggacgactgcaggagatcataaaacgcgccaccgaatattacgacagctacgaattctcgaaggtttacaatctcgtggtgaagtactgcacaacagaactcagctccctctacctggacgtcgtaaaggacagactctacgtggaagcgaaggattcaatatacagaagatcggcgcagacggtgatgcacgaaatactcatctctctgatgaagatccttgcacccatcatgactttcacgatggaagaggtttactcccatcttcatgaaaaggacagaaaatacaaaacggttcaggcagaatactggccagagtacagagaagagttcatcgatagaaagctcatggaagacttcgaaaaactcctctccataagagaagacgtgttgaaggccctcgaggagaagagacagcaagatgtgatcggtcactccctcgacgctgaggtcgtacttgtacccaaaaatgacaccatcaaagcgcttctggaaaaatacagagacatactcgaagaactcttcatagtatcgaaggtatccctttcggacgcttcgggagaattgaaaggagaactggtagaggtgaccgtcaaacacgcagagggtgaaaaatgtcagaggtgctggaaatacacaacggagatctctgcttctgaagattttccgggggtttgtccaaggtgtctggctgttttgaaaggagaaaggaagtga	2760	cagtgcGGTCTCccatggaatacaaaaacacactgaac	tttggAAGCTTtcacttcctttctcctttcaaaac	cagtgcGGTCTCccatggaatacaaaaacacactgaacttgccaaaaacttcatttccaatgaaggcaaacttggtgaacaaagaaaaagtttttctcgaagaatgggaaaagatggacctttacaactacgtactcgaacagagaaaaggaaaacccctttttgtcctgcacgatggacccccttacgcaaacggtcacatccacattggaacggccctgaacaagattctgaaggacatcgtcgtcaaatacaaaacgatgagaggatacagggcaccgtacgttcctggctgggacacccacggtcttcccatagaacacagggtgtctcaggaactgggtgaaaagataaaagaaatgtcacccgcagagatcagaaagaagtgtgaggaattcgctctcagattcgttgatatacagagagaagaatttaaaagactcggcgttcggggagactgggaaaatccatacatcacgttgaagccagactacgaggtgaaaatactggatgttttcaaaacccttgtagaacagggaaacgtttacagatctttgaaacccatctactggtgtccgagatgcagaactgccctcgcggaagcggaaatcgaataccacgatcataaatctccttccatatacgtgaagttcagatccaaagaagatccgaatttcttcatcgtgatatggaccacaacaccctggacactgccggcaaacgtcggtatcgctctgcaccccgattacgaatactcggtggtgaaagtcggagaagagaaatgggtcatcgcaacggatcttctcgatgctttttcgaaggagactggaatcgactgttccaatgtcgtcgagaaaataaaaggaaaagatctggaaggaaaagagttcgttcatcccatcttcgatgacaggacctccagagtcattctggccgattacgtatctctcgaaacaggaacaggatgtgttcacatagcccccggtcacggtgaagaagactacatctacggtcatgtgcaatacggtcttcccatcgtctctcccgttgatgaggagggacgtttcacggaagaagcgggaaaatacaagggaatgttcatagaagatgccaacgaggtgatcatagaggatctgaagagaaagggtattctcgttcacgcttcaagtatcacacactcctacccgcactgctggcgctgtaaaggacctgtgatcttcagagcaactgagcagtggttcatttctgtggatcacaacaacctcaggcagaaagtgctggaagaaatagacaaggtgaagtggataccggaatggggccggaacagaatcagatccatggtggaggaaaggcccgactggtgtatctcaagacagcgtgtctggggaactcccataccagcggtgaaatgtaaagaatgcggagaagtggtactcgatccgaaggtgatagaacacttcatgaaaatcgttgaaaaagaaggtacaaacgcctggtttgaaaaagaggtggaagaactcatccccgaagattttgtgtgtccaaaatgcggcaagcgatctttcgaaaaaatgctggatacactcgacgtatggatagattctggtgcctccttcgaatacataaccacgaagagagaagatcacccgttccccctcgacatgtaccttgaaggtagcgatcagcacaggggatggttccattcttccatcttccttgccgtcgcaaagagggggtccgcaccttacaaagaagtgctaacacacggattcataaaggacgaacagggaagaaagatgagcaagtccctcggaaacgtagtggatcccatggaagtggtggaaaaatacggtgccgagattctgagactgtggcttgcgagcagcgactacttcaacgacataaagatatccatgaggatcgtcgaacaacaaacggaagtttacaggaagataagaaacacgttccgattcctccttggaaatctagaggactttgatcctgaacttgacagagttcctcatgaaaaactcttaacgatcgacagatgggctcttggacgactgcaggagatcataaaacgcgccaccgaatattacgacagctacgaattctcgaaggtttacaatctcgtggtgaagtactgcacaacagaactcagctccctctacctggacgtcgtaaaggacagactctacgtggaagcgaaggattcaatatacagaagatcggcgcagacggtgatgcacgaaatactcatctctctgatgaagatccttgcacccatcatgactttcacgatggaagaggtttactcccatcttcatgaaaaggacagaaaatacaaaacggttcaggcagaatactggccagagtacagagaagagttcatcgatagaaagctcatggaagacttcgaaaaactcctctccataagagaagacgtgttgaaggccctcgaggagaagagacagcaagatgtgatcggtcactccctcgacgctgaggtcgtacttgtacccaaaaatgacaccatcaaagcgcttctggaaaaatacagagacatactcgaagaactcttcatagtatcgaaggtatccctttcggacgcttcgggagaattgaaaggagaactggtagaggtgaccgtcaaacacgcagagggtgaaaaatgtcagaggtgctggaaatacacaacggagatctctgcttctgaagattttccgggggtttgtccaaggtgtctggctgttttgaaaggagaaaggaagtgaAAGCTTccaaa	catggaatacaaaaacacactgaacttgccaaaaacttcatttccaatgaaggcaaacttggtgaacaaagaaaaagtttttctcgaagaatgggaaaagatggacctttacaactacgtactcgaacagagaaaaggaaaacccctttttgtcctgcacgatggacccccttacgcaaacggtcacatccacattggaacggccctgaacaagattctgaaggacatcgtcgtcaaatacaaaacgatgagaggatacagggcaccgtacgttcctggctgggacacccacggtcttcccatagaacacagggtgtctcaggaactgggtgaaaagataaaagaaatgtcacccgcagagatcagaaagaagtgtgaggaattcgctctcagattcgttgatatacagagagaagaatttaaaagactcggcgttcggggagactgggaaaatccatacatcacgttgaagccagactacgaggtgaaaatactggatgttttcaaaacccttgtagaacagggaaacgtttacagatctttgaaacccatctactggtgtccgagatgcagaactgccctcgcggaagcggaaatcgaataccacgatcataaatctccttccatatacgtgaagttcagatccaaagaagatccgaatttcttcatcgtgatatggaccacaacaccctggacactgccggcaaacgtcggtatcgctctgcaccccgattacgaatactcggtggtgaaagtcggagaagagaaatgggtcatcgcaacggatcttctcgatgctttttcgaaggagactggaatcgactgttccaatgtcgtcgagaaaataaaaggaaaagatctggaaggaaaagagttcgttcatcccatcttcgatgacaggacctccagagtcattctggccgattacgtatctctcgaaacaggaacaggatgtgttcacatagcccccggtcacggtgaagaagactacatctacggtcatgtgcaatacggtcttcccatcgtctctcccgttgatgaggagggacgtttcacggaagaagcgggaaaatacaagggaatgttcatagaagatgccaacgaggtgatcatagaggatctgaagagaaagggtattctcgttcacgcttcaagtatcacacactcctacccgcactgctggcgctgtaaaggacctgtgatcttcagagcaactgagcagtggttcatttctgtggatcacaacaacctcaggcagaaagtgctggaagaaatagacaaggtgaagtggataccggaatggggccggaacagaatcagatccatggtggaggaaaggcccgactggtgtatctcaagacagcgtgtctggggaactcccataccagcggtgaaatgtaaagaatgcggagaagtggtactcgatccgaaggtgatagaacacttcatgaaaatcgttgaaaaagaaggtacaaacgcctggtttgaaaaagaggtggaagaactcatccccgaagattttgtgtgtccaaaatgcggcaagcgatctttcgaaaaaatgctggatacactcgacgtatggatagattctggtgcctccttcgaatacataaccacgaagagagaagatcacccgttccccctcgacatgtaccttgaaggtagcgatcagcacaggggatggttccattcttccatcttccttgccgtcgcaaagagggggtccgcaccttacaaagaagtgctaacacacggattcataaaggacgaacagggaagaaagatgagcaagtccctcggaaacgtagtggatcccatggaagtggtggaaaaatacggtgccgagattctgagactgtggcttgcgagcagcgactacttcaacgacataaagatatccatgaggatcgtcgaacaacaaacggaagtttacaggaagataagaaacacgttccgattcctccttggaaatctagaggactttgatcctgaacttgacagagttcctcatgaaaaactcttaacgatcgacagatgggctcttggacgactgcaggagatcataaaacgcgccaccgaatattacgacagctacgaattctcgaaggtttacaatctcgtggtgaagtactgcacaacagaactcagctccctctacctggacgtcgtaaaggacagactctacgtggaagcgaaggattcaatatacagaagatcggcgcagacggtgatgcacgaaatactcatctctctgatgaagatccttgcacccatcatgactttcacgatggaagaggtttactcccatcttcatgaaaaggacagaaaatacaaaacggttcaggcagaatactggccagagtacagagaagagttcatcgatagaaagctcatggaagacttcgaaaaactcctctccataagagaagacgtgttgaaggccctcgaggagaagagacagcaagatgtgatcggtcactccctcgacgctgaggtcgtacttgtacccaaaaatgacaccatcaaagcgcttctggaaaaatacagagacatactcgaagaactcttcatagtatcgaaggtatccctttcggacgcttcgggagaattgaaaggagaactggtagaggtgaccgtcaaacacgcagagggtgaaaaatgtcagaggtgctggaaatacacaacggagatctctgcttctgaagattttccgggggtttgtccaaggtgtctggctgttttgaaaggagaaaggaagtgaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACcatggaatacaaaaacacactgaacttgccaaaaacttcatttccaatgaaggcaaacttggtgaacaaagaaaaagtttttctcgaagaatgggaaaagatggacctttacaactacgtactcgaacagagaaaaggaaaacccctttttgtcctgcacgatggacccccttacgcaaacggtcacatccacattggaacggccctgaacaagattctgaaggacatcgtcgtcaaatacaaaacgatgagaggatacagggcaccgtacgttcctggctgggacacccacggtcttcccatagaacacagggtgtctcaggaactgggtgaaaagataaaagaaatgtcacccgcagagatcagaaagaagtgtgaggaattcgctctcagattcgttgatatacagagagaagaatttaaaagactcggcgttcggggagactgggaaaatccatacatcacgttgaagccagactacgaggtgaaaatactggatgttttcaaaacccttgtagaacagggaaacgtttacagatctttgaaacccatctactggtgtccgagatgcagaactgccctcgcggaagcggaaatcgaataccacgatcataaatctccttccatatacgtgaagttcagatccaaagaagatccgaatttcttcatcgtgatatggaccacaacaccctggacactgccggcaaacgtcggtatcgctctgcaccccgattacgaatactcggtggtgaaagtcggagaagagaaatgggtcatcgcaacggatcttctcgatgctttttcgaaggagactggaatcgactgttccaatgtcgtcgagaaaataaaaggaaaagatctggaaggaaaagagttcgttcatcccatcttcgatgacaggacctccagagtcattctggccgattacgtatctctcgaaacaggaacaggatgtgttcacatagcccccggtcacggtgaagaagactacatctacggtcatgtgcaatacggtcttcccatcgtctctcccgttgatgaggagggacgtttcacggaagaagcgggaaaatacaagggaatgttcatagaagatgccaacgaggtgatcatagaggatctgaagagaaagggtattctcgttcacgcttcaagtatcacacactcctacccgcactgctggcgctgtaaaggacctgtgatcttcagagcaactgagcagtggttcatttctgtggatcacaacaacctcaggcagaaagtgctggaagaaatagacaaggtgaagtggataccggaatggggccggaacagaatcagatccatggtggaggaaaggcccgactggtgtatctcaagacagcgtgtctggggaactcccataccagcggtgaaatgtaaagaatgcggagaagtggtactcgatccgaaggtgatagaacacttcatgaaaatcgttgaaaaagaaggtacaaacgcctggtttgaaaaagaggtggaagaactcatccccgaagattttgtgtgtccaaaatgcggcaagcgatctttcgaaaaaatgctggatacactcgacgtatggatagattctggtgcctccttcgaatacataaccacgaagagagaagatcacccgttccccctcgacatgtaccttgaaggtagcgatcagcacaggggatggttccattcttccatcttccttgccgtcgcaaagagggggtccgcaccttacaaagaagtgctaacacacggattcataaaggacgaacagggaagaaagatgagcaagtccctcggaaacgtagtggatcccatggaagtggtggaaaaatacggtgccgagattctgagactgtggcttgcgagcagcgactacttcaacgacataaagatatccatgaggatcgtcgaacaacaaacggaagtttacaggaagataagaaacacgttccgattcctccttggaaatctagaggactttgatcctgaacttgacagagttcctcatgaaaaactcttaacgatcgacagatgggctcttggacgactgcaggagatcataaaacgcgccaccgaatattacgacagctacgaattctcgaaggtttacaatctcgtggtgaagtactgcacaacagaactcagctccctctacctggacgtcgtaaaggacagactctacgtggaagcgaaggattcaatatacagaagatcggcgcagacggtgatgcacgaaatactcatctctctgatgaagatccttgcacccatcatgactttcacgatggaagaggtttactcccatcttcatgaaaaggacagaaaatacaaaacggttcaggcagaatactggccagagtacagagaagagttcatcgatagaaagctcatggaagacttcgaaaaactcctctccataagagaagacgtgttgaaggccctcgaggagaagagacagcaagatgtgatcggtcactccctcgacgctgaggtcgtacttgtacccaaaaatgacaccatcaaagcgcttctggaaaaatacagagacatactcgaagaactcttcatagtatcgaaggtatccctttcggacgcttcgggagaattgaaaggagaactggtagaggtgaccgtcaaacacgcagagggtgaaaaatgtcagaggtgctggaaatacacaacggagatctctgcttctgaagattttccgggggtttgtccaaggtgtctggctgttttgaaaggagaaaggaagtgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	BsaI/HindIII	2785	2762	internal ncoI site	isoleucine	4, 1.3, .9, .5
10	pBad myc/hisA		TM-K	lysine	1685041..1686549	GTGCTGAAAGAGTTCAAAGAACAACGACTCAAAGAGATACAGGAACTTCGTTCTATGGGTATAGAGCCATATCCTTACAAATTCGAAAAGGAACTCACGGCCAGAGAAATAAGGGAAAAGTACGACTATCTTCAAGCTGGAGAAGTTCTTGAATCTGAAAAGCTCTCTTTCGCAGGGAGAGTCATGTCGATACGTCACCACGGAAAGACGGCCTTCTTTCACATGAAAGATGACACGGGCCGGATCCAGGCTTACATAAAAGCAGATTCTGTTGGGAAGGAAAAGATGGATCTCTTCAAAAGACACGTCAAAATAGGTGACTTTGTAGGAGTAAGAGGCTTTCCGTTCAAAAGCAAAACCGGAGAGTTGACCATTTACGTTCAAGAATACACGCTTCTGAGCAAGGCTCTGCGGCCTCTGCCCGAGAAGTGGCACGGCATAAAAGATAAGGAAATCATATACAGGCAGAGGTACCTCGAACTCATCGTGAACGACGAGGCTATTGAGAGGTTCAAAAAGCGATTCAAGGCCGTCCGTGTGATACGGGAATTTCTCAACAGCAGGGGATTCATCGAAGTGGAAACACCTATTCTTCATTACGTCACGGGTGGTGCGGAAGCGAGGCCTTTTGTGACCCATCTCAACGTTTTTGATATCGATATGTATTTGAGAATCGCACCGGAGCTTTACCTGAAAAGGTTGATCGTCGGCGGTTTTGAAAAGATATACGAGATAGGAAAGAACTTCAGAAACGAAGGAATATCTTACAAACACAGTCCCGAGTTCACCAGCATAGAGATCTACCAGGCTTACGCGGATTACAACGATATGATGGATCTCACTGAAGAGTTGATAGTGGAAGTTGTGAAAAGAACATGTGGCACCTTGAAAATCTCCTACCAAGGCAAGGAGATAGACTTCACACCACCCTGGAAAAGAGTGAGAATGAGAGACTTTCTCAAAGAAAAACTCGGGGTGGACATCCTCGAAGATCCCGATGAAGTGTTGTTGAAGAAGCTCGAAGAACATGGGGTAGAACTGGAAATAAAGAACAGAGCACACCTCATAGACAAACTCAGAGATCTGGTGGAAGAAGAGCTGGTGAACCCCACCTTCATAATCGATCATCCTGTGGTGATTTCCCCTCTTGCCAAAAGACACCGAGAGGATCCTAGACTCACGGAAAGGTTCGAACTCATCATATTCGGAAGAGAGATCGCGAACGCGTTCAGTGAGCTGAACGATCCCGTTGATCAATACCAGAGATTTCTCGAACAGGCGAAAATGAGAGAAGAGGGAGATGAAGAAGCACACATGATGGATCTCGATTTTGTGAGGGCCCTCGAGTATGGTATGCCTCCCACGGGAGGACTGGGAATCGGTCTTGACAGGCTCTTCATGTTCATCACGGATTCCCCCACAATAAGGGATGTGATTCCCTTCCCAATTGTGAAACCCAAAAAGTTCGAGGAAGAAGAGGCGGAATTCGAAGGAGGGTTTGAAGAATGA	1509	ccaaaCCATGGcgctgaaagagttcaaagaacaacgac	tttggAAGCTTtcattcttcaaaccctccttcgaat	ccaaaCCATGGcgctgaaagagttcaaagaacaacgacTCAAAGAGATACAGGAACTTCGTTCTATGGGTATAGAGCCATATCCTTACAAATTCGAAAAGGAACTCACGGCCAGAGAAATAAGGGAAAAGTACGACTATCTTCAAGCTGGAGAAGTTCTTGAATCTGAAAAGCTCTCTTTCGCAGGGAGAGTCATGTCGATACGTCACCACGGAAAGACGGCCTTCTTTCACATGAAAGATGACACGGGCCGGATCCAGGCTTACATAAAAGCAGATTCTGTTGGGAAGGAAAAGATGGATCTCTTCAAAAGACACGTCAAAATAGGTGACTTTGTAGGAGTAAGAGGCTTTCCGTTCAAAAGCAAAACCGGAGAGTTGACCATTTACGTTCAAGAATACACGCTTCTGAGCAAGGCTCTGCGGCCTCTGCCCGAGAAGTGGCACGGCATAAAAGATAAGGAAATCATATACAGGCAGAGGTACCTCGAACTCATCGTGAACGACGAGGCTATTGAGAGGTTCAAAAAGCGATTCAAGGCCGTCCGTGTGATACGGGAATTTCTCAACAGCAGGGGATTCATCGAAGTGGAAACACCTATTCTTCATTACGTCACGGGTGGTGCGGAAGCGAGGCCTTTTGTGACCCATCTCAACGTTTTTGATATCGATATGTATTTGAGAATCGCACCGGAGCTTTACCTGAAAAGGTTGATCGTCGGCGGTTTTGAAAAGATATACGAGATAGGAAAGAACTTCAGAAACGAAGGAATATCTTACAAACACAGTCCCGAGTTCACCAGCATAGAGATCTACCAGGCTTACGCGGATTACAACGATATGATGGATCTCACTGAAGAGTTGATAGTGGAAGTTGTGAAAAGAACATGTGGCACCTTGAAAATCTCCTACCAAGGCAAGGAGATAGACTTCACACCACCCTGGAAAAGAGTGAGAATGAGAGACTTTCTCAAAGAAAAACTCGGGGTGGACATCCTCGAAGATCCCGATGAAGTGTTGTTGAAGAAGCTCGAAGAACATGGGGTAGAACTGGAAATAAAGAACAGAGCACACCTCATAGACAAACTCAGAGATCTGGTGGAAGAAGAGCTGGTGAACCCCACCTTCATAATCGATCATCCTGTGGTGATTTCCCCTCTTGCCAAAAGACACCGAGAGGATCCTAGACTCACGGAAAGGTTCGAACTCATCATATTCGGAAGAGAGATCGCGAACGCGTTCAGTGAGCTGAACGATCCCGTTGATCAATACCAGAGATTTCTCGAACAGGCGAAAATGAGAGAAGAGGGAGATGAAGAAGCACACATGATGGATCTCGATTTTGTGAGGGCCCTCGAGTATGGTATGCCTCCCACGGGAGGACTGGGAATCGGTCTTGACAGGCTCTTCATGTTCATCACGGATTCCCCCACAATAAGGGATGTGATTCCCTTCCCAATTGTGAAACCCAAAAAGTTCGAGGAAGAAGAGGCGGAattcgaaggagggtttgaagaatgaAAGCTTccaaa	CATGGcgctgaaagagttcaaagaacaacgacTCAAAGAGATACAGGAACTTCGTTCTATGGGTATAGAGCCATATCCTTACAAATTCGAAAAGGAACTCACGGCCAGAGAAATAAGGGAAAAGTACGACTATCTTCAAGCTGGAGAAGTTCTTGAATCTGAAAAGCTCTCTTTCGCAGGGAGAGTCATGTCGATACGTCACCACGGAAAGACGGCCTTCTTTCACATGAAAGATGACACGGGCCGGATCCAGGCTTACATAAAAGCAGATTCTGTTGGGAAGGAAAAGATGGATCTCTTCAAAAGACACGTCAAAATAGGTGACTTTGTAGGAGTAAGAGGCTTTCCGTTCAAAAGCAAAACCGGAGAGTTGACCATTTACGTTCAAGAATACACGCTTCTGAGCAAGGCTCTGCGGCCTCTGCCCGAGAAGTGGCACGGCATAAAAGATAAGGAAATCATATACAGGCAGAGGTACCTCGAACTCATCGTGAACGACGAGGCTATTGAGAGGTTCAAAAAGCGATTCAAGGCCGTCCGTGTGATACGGGAATTTCTCAACAGCAGGGGATTCATCGAAGTGGAAACACCTATTCTTCATTACGTCACGGGTGGTGCGGAAGCGAGGCCTTTTGTGACCCATCTCAACGTTTTTGATATCGATATGTATTTGAGAATCGCACCGGAGCTTTACCTGAAAAGGTTGATCGTCGGCGGTTTTGAAAAGATATACGAGATAGGAAAGAACTTCAGAAACGAAGGAATATCTTACAAACACAGTCCCGAGTTCACCAGCATAGAGATCTACCAGGCTTACGCGGATTACAACGATATGATGGATCTCACTGAAGAGTTGATAGTGGAAGTTGTGAAAAGAACATGTGGCACCTTGAAAATCTCCTACCAAGGCAAGGAGATAGACTTCACACCACCCTGGAAAAGAGTGAGAATGAGAGACTTTCTCAAAGAAAAACTCGGGGTGGACATCCTCGAAGATCCCGATGAAGTGTTGTTGAAGAAGCTCGAAGAACATGGGGTAGAACTGGAAATAAAGAACAGAGCACACCTCATAGACAAACTCAGAGATCTGGTGGAAGAAGAGCTGGTGAACCCCACCTTCATAATCGATCATCCTGTGGTGATTTCCCCTCTTGCCAAAAGACACCGAGAGGATCCTAGACTCACGGAAAGGTTCGAACTCATCATATTCGGAAGAGAGATCGCGAACGCGTTCAGTGAGCTGAACGATCCCGTTGATCAATACCAGAGATTTCTCGAACAGGCGAAAATGAGAGAAGAGGGAGATGAAGAAGCACACATGATGGATCTCGATTTTGTGAGGGCCCTCGAGTATGGTATGCCTCCCACGGGAGGACTGGGAATCGGTCTTGACAGGCTCTTCATGTTCATCACGGATTCCCCCACAATAAGGGATGTGATTCCCTTCCCAATTGTGAAACCCAAAAAGTTCGAGGAAGAAGAGGCGGAattcgaaggagggtttgaagaatgaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGcgctgaaagagttcaaagaacaacgacTCAAAGAGATACAGGAACTTCGTTCTATGGGTATAGAGCCATATCCTTACAAATTCGAAAAGGAACTCACGGCCAGAGAAATAAGGGAAAAGTACGACTATCTTCAAGCTGGAGAAGTTCTTGAATCTGAAAAGCTCTCTTTCGCAGGGAGAGTCATGTCGATACGTCACCACGGAAAGACGGCCTTCTTTCACATGAAAGATGACACGGGCCGGATCCAGGCTTACATAAAAGCAGATTCTGTTGGGAAGGAAAAGATGGATCTCTTCAAAAGACACGTCAAAATAGGTGACTTTGTAGGAGTAAGAGGCTTTCCGTTCAAAAGCAAAACCGGAGAGTTGACCATTTACGTTCAAGAATACACGCTTCTGAGCAAGGCTCTGCGGCCTCTGCCCGAGAAGTGGCACGGCATAAAAGATAAGGAAATCATATACAGGCAGAGGTACCTCGAACTCATCGTGAACGACGAGGCTATTGAGAGGTTCAAAAAGCGATTCAAGGCCGTCCGTGTGATACGGGAATTTCTCAACAGCAGGGGATTCATCGAAGTGGAAACACCTATTCTTCATTACGTCACGGGTGGTGCGGAAGCGAGGCCTTTTGTGACCCATCTCAACGTTTTTGATATCGATATGTATTTGAGAATCGCACCGGAGCTTTACCTGAAAAGGTTGATCGTCGGCGGTTTTGAAAAGATATACGAGATAGGAAAGAACTTCAGAAACGAAGGAATATCTTACAAACACAGTCCCGAGTTCACCAGCATAGAGATCTACCAGGCTTACGCGGATTACAACGATATGATGGATCTCACTGAAGAGTTGATAGTGGAAGTTGTGAAAAGAACATGTGGCACCTTGAAAATCTCCTACCAAGGCAAGGAGATAGACTTCACACCACCCTGGAAAAGAGTGAGAATGAGAGACTTTCTCAAAGAAAAACTCGGGGTGGACATCCTCGAAGATCCCGATGAAGTGTTGTTGAAGAAGCTCGAAGAACATGGGGTAGAACTGGAAATAAAGAACAGAGCACACCTCATAGACAAACTCAGAGATCTGGTGGAAGAAGAGCTGGTGAACCCCACCTTCATAATCGATCATCCTGTGGTGATTTCCCCTCTTGCCAAAAGACACCGAGAGGATCCTAGACTCACGGAAAGGTTCGAACTCATCATATTCGGAAGAGAGATCGCGAACGCGTTCAGTGAGCTGAACGATCCCGTTGATCAATACCAGAGATTTCTCGAACAGGCGAAAATGAGAGAAGAGGGAGATGAAGAAGCACACATGATGGATCTCGATTTTGTGAGGGCCCTCGAGTATGGTATGCCTCCCACGGGAGGACTGGGAATCGGTCTTGACAGGCTCTTCATGTTCATCACGGATTCCCCCACAATAAGGGATGTGATTCCCTTCCCAATTGTGAAACCCAAAAAGTTCGAGGAAGAAGAGGCGGAattcgaaggagggtttgaagaatgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	NcoI/HindIII	1530	1514		lysine	4, 1.5
11	pBad myc/hisA		TM-E (2)	glutamic acid	1370561..1371970	ttggtaagggtgagattcgcaccgagccccactggctttctccacgttggaggagctcgaaccgctctgttcaactttcttttcgcacggaaagaaaaaggaaagttcatcctcagaatagaagacaccgatttggagagatctgagagggagtacgaagagaagctcatggaatctctcaggtggcttggtcttctgtgggatgagggaccggacgtgggaggagaccacggtccatacaggcagagcgagagagtggagatatacagagaacacgctgaaaggcttgtaaaggaggggaaggcctattacgtttacgcttaccctgaagagatcgaagagatgagggagaaactcctctctgaaggaaaggcaccacactactcccaggagatgttcgaaaagtttgatactcccgagagaagaagggaatacgaagaaaagggtttgaggccggctgtgtttttcaagatgcccagaaaagactatgtgttgaacgatgtggtgaaaggtgaagttgtgttcaagacaggagcaattggggattttgtgataatgagaagcaacggtcttcccacctacaatttcgcctgtgtggtggatgacatgctcatggaaataacacacgttatccgtggagacgaccatctctcaaatacactgagacagctcgctctctatgaagcgtttgaaaaggctccccccgtttttgcgcacgtttccaccatactgggacccgatggaaaaaaactgagcaagagacatggagccacttctgtggaagcgttcagagacatgggatatcttccggaggcgcttgtcaactatctggcacttcttggatggtctcacccagagggtaaagaactcctcactctcgaagaattgatttcttcgttttctctggacaggctcagcccgaaccctgcgattttcgacccacagaagttgaaatggatgaacggttactatttgaggaacatgccgattgagaagctcgcagaactggcaaagccgttctttgagaaagccgggataaaaataatcgacgaggagtactttaaaaaggtattggagatcacaaaagagagagtagaggtgctttcagaatttcctgaagagtcccgcttcttctttgaagatccagcacctgttgaaataccagaagaaatgaaagaagtgttttcacagctcaaagaagagctccagaacgttcggtggaccatggaagaaatcacacctgtcttcaagaaggtactgaaacaacacggtgtaaagcccaaagaattctatatgacgttgagaagagttctgactggtagagaagaaggtcctgaactcgtcaacattattcctcttcttggaaaggagatctttttgagaagaatagaaagatctctggggggatga	1410	ccaaaCGTCTCccatggtaagggtgagattcgcaccg	tttggAAGCTTtcatccccccagagatctttctattct	ccaaaCGTCTCccatggtaagggtgagattcgcaccgagccccactggctttctccacgttggaggagctcgaaccgctctgttcaactttcttttcgcacggaaagaaaaaggaaagttcatcctcagaatagaagacaccgatttggagagatctgagagggagtacgaagagaagctcatggaatctctcaggtggcttggtcttctgtgggatgagggaccggacgtgggaggagaccacggtccatacaggcagagcgagagagtggagatatacagagaacacgctgaaaggcttgtaaaggaggggaaggcctattacgtttacgcttaccctgaagagatcgaagagatgagggagaaactcctctctgaaggaaaggcaccacactactcccaggagatgttcgaaaagtttgatactcccgagagaagaagggaatacgaagaaaagggtttgaggccggctgtgtttttcaagatgcccagaaaagactatgtgttgaacgatgtggtgaaaggtgaagttgtgttcaagacaggagcaattggggattttgtgataatgagaagcaacggtcttcccacctacaatttcgcctgtgtggtggatgacatgctcatggaaataacacacgttatccgtggagacgaccatctctcaaatacactgagacagctcgctctctatgaagcgtttgaaaaggctccccccgtttttgcgcacgtttccaccatactgggacccgatggaaaaaaactgagcaagagacatggagccacttctgtggaagcgttcagagacatgggatatcttccggaggcgcttgtcaactatctggcacttcttggatggtctcacccagagggtaaagaactcctcactctcgaagaattgatttcttcgttttctctggacaggctcagcccgaaccctgcgattttcgacccacagaagttgaaatggatgaacggttactatttgaggaacatgccgattgagaagctcgcagaactggcaaagccgttctttgagaaagccgggataaaaataatcgacgaggagtactttaaaaaggtattggagatcacaaaagagagagtagaggtgctttcagaatttcctgaagagtcccgcttcttctttgaagatccagcacctgttgaaataccagaagaaatgaaagaagtgttttcacagctcaaagaagagctccagaacgttcggtggaccatggaagaaatcacacctgtcttcaagaaggtactgaaacaacacggtgtaaagcccaaagaattctatatgacgttgagaagagttctgactggtagagaagaaggtcctgaactcgtcaacattattcctcttcttggaaaggagatctttttgagaagaatagaaagatctctggggggatgaAAGCTTccaaa	catggtaagggtgagattcgcaccgagccccactggctttctccacgttggaggagctcgaaccgctctgttcaactttcttttcgcacggaaagaaaaaggaaagttcatcctcagaatagaagacaccgatttggagagatctgagagggagtacgaagagaagctcatggaatctctcaggtggcttggtcttctgtgggatgagggaccggacgtgggaggagaccacggtccatacaggcagagcgagagagtggagatatacagagaacacgctgaaaggcttgtaaaggaggggaaggcctattacgtttacgcttaccctgaagagatcgaagagatgagggagaaactcctctctgaaggaaaggcaccacactactcccaggagatgttcgaaaagtttgatactcccgagagaagaagggaatacgaagaaaagggtttgaggccggctgtgtttttcaagatgcccagaaaagactatgtgttgaacgatgtggtgaaaggtgaagttgtgttcaagacaggagcaattggggattttgtgataatgagaagcaacggtcttcccacctacaatttcgcctgtgtggtggatgacatgctcatggaaataacacacgttatccgtggagacgaccatctctcaaatacactgagacagctcgctctctatgaagcgtttgaaaaggctccccccgtttttgcgcacgtttccaccatactgggacccgatggaaaaaaactgagcaagagacatggagccacttctgtggaagcgttcagagacatgggatatcttccggaggcgcttgtcaactatctggcacttcttggatggtctcacccagagggtaaagaactcctcactctcgaagaattgatttcttcgttttctctggacaggctcagcccgaaccctgcgattttcgacccacagaagttgaaatggatgaacggttactatttgaggaacatgccgattgagaagctcgcagaactggcaaagccgttctttgagaaagccgggataaaaataatcgacgaggagtactttaaaaaggtattggagatcacaaaagagagagtagaggtgctttcagaatttcctgaagagtcccgcttcttctttgaagatccagcacctgttgaaataccagaagaaatgaaagaagtgttttcacagctcaaagaagagctccagaacgttcggtggaccatggaagaaatcacacctgtcttcaagaaggtactgaaacaacacggtgtaaagcccaaagaattctatatgacgttgagaagagttctgactggtagagaagaaggtcctgaactcgtcaacattattcctcttcttggaaaggagatctttttgagaagaatagaaagatctctggggggatgaA	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACcatggtaagggtgagattcgcaccgagccccactggctttctccacgttggaggagctcgaaccgctctgttcaactttcttttcgcacggaaagaaaaaggaaagttcatcctcagaatagaagacaccgatttggagagatctgagagggagtacgaagagaagctcatggaatctctcaggtggcttggtcttctgtgggatgagggaccggacgtgggaggagaccacggtccatacaggcagagcgagagagtggagatatacagagaacacgctgaaaggcttgtaaaggaggggaaggcctattacgtttacgcttaccctgaagagatcgaagagatgagggagaaactcctctctgaaggaaaggcaccacactactcccaggagatgttcgaaaagtttgatactcccgagagaagaagggaatacgaagaaaagggtttgaggccggctgtgtttttcaagatgcccagaaaagactatgtgttgaacgatgtggtgaaaggtgaagttgtgttcaagacaggagcaattggggattttgtgataatgagaagcaacggtcttcccacctacaatttcgcctgtgtggtggatgacatgctcatggaaataacacacgttatccgtggagacgaccatctctcaaatacactgagacagctcgctctctatgaagcgtttgaaaaggctccccccgtttttgcgcacgtttccaccatactgggacccgatggaaaaaaactgagcaagagacatggagccacttctgtggaagcgttcagagacatgggatatcttccggaggcgcttgtcaactatctggcacttcttggatggtctcacccagagggtaaagaactcctcactctcgaagaattgatttcttcgttttctctggacaggctcagcccgaaccctgcgattttcgacccacagaagttgaaatggatgaacggttactatttgaggaacatgccgattgagaagctcgcagaactggcaaagccgttctttgagaaagccgggataaaaataatcgacgaggagtactttaaaaaggtattggagatcacaaaagagagagtagaggtgctttcagaatttcctgaagagtcccgcttcttctttgaagatccagcacctgttgaaataccagaagaaatgaaagaagtgttttcacagctcaaagaagagctccagaacgttcggtggaccatggaagaaatcacacctgtcttcaagaaggtactgaaacaacacggtgtaaagcccaaagaattctatatgacgttgagaagagttctgactggtagagaagaaggtcctgaactcgtcaacattattcctcttcttggaaaggagatctttttgagaagaatagaaagatctctggggggatgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	Bsmbi/HindIII	1434	1412	internal ncoI site/bsmbi site	glutamic acid (2)	4, 1.2, .2
12
13			pBad myc/hisA	vector		AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGATCCGAGCTCGAGATCTGCAGCTGGTACCATATGGGAATTCGAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG	4094
14						CATGGATCCGAGCTCGAGATCTGCAGCTGGTACCATATGGGAATTCGA	48
15						AGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAGAAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAAC	4046
16	require soeing							forward oligo1	reverse oligo2	internal forward oligo1	internal reverse oligo	PCR 1	PCR2	PCR product	cut sequence		PCR1 size	PCR2 size	final PCR size		plasmid cut sizes with ncoI nad hindIII	plasmid seq
17																	0	0	0	internal ncoI site
18	pBad myc/hisA		TM-A	alanine	1410448..1413039	atgagatacatgacttctgaagagatcagagaagcctttttgaaattctttgagaaaaagggacacaagatactaccgagcgcttctctcataccggacgatcctcagcttctcttcaccgtagccggtatggttcctttcaaacctattttctggggcaaagtcgagcctgtttacacgagggtggctacctgtcagaagtgtttgagaaccgttgatatagagaacgtgggaaagactccaaggcatcatacgttctttgaaatgctcggtaatttctcctttggagactatttcaaagaagaagccatagagtgggcctgggagttcctcacccaggtgcttggggtccccgaagaaaaactgtgggtctccgtctacgaggaagacgaagaggccttcagaatctggaacgaaaagataggtcttcccgagaagagaattttgagaatgggaaaagaggacaatttctgggggccggctgggccaacagggccatgtggtcccgatacggagatcttttacgatacagggtattcgaagggctgtcctgagggcgaagggtgtactcctgccaattctgaaggacgattcgtggagatatggaaccttgttttcaccgagtattaccaggacgaagaaggaaagctccatcctcttccgagaaagaacatagatacgggagctggtcttgaaaggttttgtgcgatgatgcagggagtttacagcaatttcgacacagatctgttccagccgataataaaacgcatagaggaactgacaggtgttggctacaaaacagatgaagaaaaagatgtttccatcagggtcatagccgatcacataagggccatcacctttttgatcagtgaaggtgttttcccttccaacgagggaagagggtacgttctcagaagaatcataaggcgtgcgatgaggcatggaatactcctcggtatgagtgaaccgttcctctacaggatcgttgatgccgtggtggagaagatgggaaaagtttacccggagatcgttcgtggtgaaggtatggtgaaagaagttctttcagcggaagaaaacagatttctaaagactctcgagcagggaatgaaagtctttgacgagatcgtcgagaagaagggaaaaatagattccgaagacgcgttcagactctacgatacatacggacttccactggagctcacccttgaaatcgcaaaggagaaaggtgtggaagtcgatgtgcaagagttcaacaaatacatggaagaacaacagcgaaagtcaagagctgctatgggcgatgtggaatttgccaggaggtacgagtaccttgaaaaactccccaaagatttccgaaccgaattcactgggtacgaaaaactcgaagatgagggcgaggtcgttctcgtagccagggacgacgaaacggtggaagaagcatcagaaggcacagttgaggtggttttttcaagaactccgttctacgcggagaagggtgggcaggtatccgacacggggatggtggaatggagagatggaaaagcacttgtggaatacgtctttgaagcttcagaaggtgtgatcgtccacagaataaagatactggatggtactctaagaagaggacagaaagtgattctcagagtggataagaagagaagagaggccacaatgagaaatcacacggcaacccatctgcttcacgcggctttgaagaaagttctgggagaccacgtgaggcaggctgggtccttggttgcacccgacagattgagattcgatttcacacatttcaaaggtctttcgagtgcagaaatagaacaggtggaggatctggtgaacgaatggatcatggaagcgattccggtcgaggtgagatacacgagctacgaagaagcggtaaagtccggagttgttgcgcttttcacggaaaaatacggagacgtggtcagggtcgtcgaggttcccggtgtgagtaaagaactctgtggcggaacgcacgtgaaaaacacagggcagataggtctgttcaagatcatctcggaggaatccgtttcgtcaggtgtgcgaagaatcgaagcggtcaccggcttcagtgctctggaacttctgagaaaccagaaaaagctgatcgatcagctgaaagaaatcctcggggcaagggaagacgagctcacggacagggtcctgagcttgagagaaaaggtgaaggaactcgaaaagaaactctctcagggaaggatttccgaagaaaggatagccatgaaacagctggaagatggtgtaaaggtcttccacggtgtcttcgaaggcgtcgaggcaaaacacctcggaggaatagcagataacgttttgaaaaaagaaggggaaggcattgttattctcttcagtaagttcgaaaacaaggtttccctcgttgtgaaggtttctgagaatcttcttggcaagtacgatgcctcctctatagcacgaaacatagcgaaggaactcggtgggaacggaggaggccgaaagaattttgcccaagcgggtggaagacatccggagaggataaaagacgttctggaacgtctcgaagagtttctgaggtga	2592	ccaaaCCATGGcgagatacatgacttctgaagagatc	tttggAAGCTTtcacctcagaaactcttcgagacg	tacgtctttgaagcCtcagaaggtgtga	tcacaccttctgaGgcttcaaagacgta	ccaaaCCATGGcgagatacatgacttctgaagagatcagagaagcctttttgaaattctttgagaaaaagggacacaagatactaccgagcgcttctctcataccggacgatcctcagcttctcttcaccgtagccggtatggttcctttcaaacctattttctggggcaaagtcgagcctgtttacacgagggtggctacctgtcagaagtgtttgagaaccgttgatatagagaacgtgggaaagactccaaggcatcatacgttctttgaaatgctcggtaatttctcctttggagactatttcaaagaagaagccatagagtgggcctgggagttcctcacccaggtgcttggggtccccgaagaaaaactgtgggtctccgtctacgaggaagacgaagaggccttcagaatctggaacgaaaagataggtcttcccgagaagagaattttgagaatgggaaaagaggacaatttctgggggccggctgggccaacagggccatgtggtcccgatacggagatcttttacgatacagggtattcgaagggctgtcctgagggcgaagggtgtactcctgccaattctgaaggacgattcgtggagatatggaaccttgttttcaccgagtattaccaggacgaagaaggaaagctccatcctcttccgagaaagaacatagatacgggagctggtcttgaaaggttttgtgcgatgatgcagggagtttacagcaatttcgacacagatctgttccagccgataataaaacgcatagaggaactgacaggtgttggctacaaaacagatgaagaaaaagatgtttccatcagggtcatagccgatcacataagggccatcacctttttgatcagtgaaggtgttttcccttccaacgagggaagagggtacgttctcagaagaatcataaggcgtgcgatgaggcatggaatactcctcggtatgagtgaaccgttcctctacaggatcgttgatgccgtggtggagaagatgggaaaagtttacccggagatcgttcgtggtgaaggtatggtgaaagaagttctttcagcggaagaaaacagatttctaaagactctcgagcagggaatgaaagtctttgacgagatcgtcgagaagaagggaaaaatagattccgaagacgcgttcagactctacgatacatacggacttccactggagctcacccttgaaatcgcaaaggagaaaggtgtggaagtcgatgtgcaagagttcaacaaatacatggaagaacaacagcgaaagtcaagagctgctatgggcgatgtggaatttgccaggaggtacgagtaccttgaaaaactccccaaagatttccgaaccgaattcactgggtacgaaaaactcgaagatgagggcgaggtcgttctcgtagccagggacgacgaaacggtggaagaagcatcagaaggcacagttgaggtggttttttcaagaactccgttctacgcggagaagggtgggcaggtatccgacacggggatggtggaatggagagatggaaaagcacttgtggaatacgtctttgaagcCtcagaaggtgtga	tacgtctttgaagcCtcagaaggtgtgatcgtccacagaataaagatactggatggtactctaagaagaggacagaaagtgattctcagagtggataagaagagaagagaggccacaatgagaaatcacacggcaacccatctgcttcacgcggctttgaagaaagttctgggagaccacgtgaggcaggctgggtccttggttgcacccgacagattgagattcgatttcacacatttcaaaggtctttcgagtgcagaaatagaacaggtggaggatctggtgaacgaatggatcatggaagcgattccggtcgaggtgagatacacgagctacgaagaagcggtaaagtccggagttgttgcgcttttcacggaaaaatacggagacgtggtcagggtcgtcgaggttcccggtgtgagtaaagaactctgtggcggaacgcacgtgaaaaacacagggcagataggtctgttcaagatcatctcggaggaatccgtttcgtcaggtgtgcgaagaatcgaagcggtcaccggcttcagtgctctggaacttctgagaaaccagaaaaagctgatcgatcagctgaaagaaatcctcggggcaagggaagacgagctcacggacagggtcctgagcttgagagaaaaggtgaaggaactcgaaaagaaactctctcagggaaggatttccgaagaaaggatagccatgaaacagctggaagatggtgtaaaggtcttccacggtgtcttcgaaggcgtcgaggcaaaacacctcggaggaatagcagataacgttttgaaaaaagaaggggaaggcattgttattctcttcagtaagttcgaaaacaaggtttccctcgttgtgaaggtttctgagaatcttcttggcaagtacgatgcctcctctatagcacgaaacatagcgaaggaactcggtgggaacggaggaggccgaaagaattttgcccaagcgggtggaagacatccggagaggataaaagacgttctggaacgtctcgaagagtttctgaggtgaAAGCTTccaaa	ccaaaCCATGGcgagatacatgacttctgaagagatcagagaagcctttttgaaattctttgagaaaaagggacacaagatactaccgagcgcttctctcataccggacgatcctcagcttctcttcaccgtagccggtatggttcctttcaaacctattttctggggcaaagtcgagcctgtttacacgagggtggctacctgtcagaagtgtttgagaaccgttgatatagagaacgtgggaaagactccaaggcatcatacgttctttgaaatgctcggtaatttctcctttggagactatttcaaagaagaagccatagagtgggcctgggagttcctcacccaggtgcttggggtccccgaagaaaaactgtgggtctccgtctacgaggaagacgaagaggccttcagaatctggaacgaaaagataggtcttcccgagaagagaattttgagaatgggaaaagaggacaatttctgggggccggctgggccaacagggccatgtggtcccgatacggagatcttttacgatacagggtattcgaagggctgtcctgagggcgaagggtgtactcctgccaattctgaaggacgattcgtggagatatggaaccttgttttcaccgagtattaccaggacgaagaaggaaagctccatcctcttccgagaaagaacatagatacgggagctggtcttgaaaggttttgtgcgatgatgcagggagtttacagcaatttcgacacagatctgttccagccgataataaaacgcatagaggaactgacaggtgttggctacaaaacagatgaagaaaaagatgtttccatcagggtcatagccgatcacataagggccatcacctttttgatcagtgaaggtgttttcccttccaacgagggaagagggtacgttctcagaagaatcataaggcgtgcgatgaggcatggaatactcctcggtatgagtgaaccgttcctctacaggatcgttgatgccgtggtggagaagatgggaaaagtttacccggagatcgttcgtggtgaaggtatggtgaaagaagttctttcagcggaagaaaacagatttctaaagactctcgagcagggaatgaaagtctttgacgagatcgtcgagaagaagggaaaaatagattccgaagacgcgttcagactctacgatacatacggacttccactggagctcacccttgaaatcgcaaaggagaaaggtgtggaagtcgatgtgcaagagttcaacaaatacatggaagaacaacagcgaaagtcaagagctgctatgggcgatgtggaatttgccaggaggtacgagtaccttgaaaaactccccaaagatttccgaaccgaattcactgggtacgaaaaactcgaagatgagggcgaggtcgttctcgtagccagggacgacgaaacggtggaagaagcatcagaaggcacagttgaggtggttttttcaagaactccgttctacgcggagaagggtgggcaggtatccgacacggggatggtggaatggagagatggaaaagcacttgtggaatacgtctttgaagcCtcagaaggtgtgatcgtccacagaataaagatactggatggtactctaagaagaggacagaaagtgattctcagagtggataagaagagaagagaggccacaatgagaaatcacacggcaacccatctgcttcacgcggctttgaagaaagttctgggagaccacgtgaggcaggctgggtccttggttgcacccgacagattgagattcgatttcacacatttcaaaggtctttcgagtgcagaaatagaacaggtggaggatctggtgaacgaatggatcatggaagcgattccggtcgaggtgagatacacgagctacgaagaagcggtaaagtccggagttgttgcgcttttcacggaaaaatacggagacgtggtcagggtcgtcgaggttcccggtgtgagtaaagaactctgtggcggaacgcacgtgaaaaacacagggcagataggtctgttcaagatcatctcggaggaatccgtttcgtcaggtgtgcgaagaatcgaagcggtcaccggcttcagtgctctggaacttctgagaaaccagaaaaagctgatcgatcagctgaaagaaatcctcggggcaagggaagacgagctcacggacagggtcctgagcttgagagaaaaggtgaaggaactcgaaaagaaactctctcagggaaggatttccgaagaaaggatagccatgaaacagctggaagatggtgtaaaggtcttccacggtgtcttcgaaggcgtcgaggcaaaacacctcggaggaatagcagataacgttttgaaaaaagaaggggaaggcattgttattctcttcagtaagttcgaaaacaaggtttccctcgttgtgaaggtttctgagaatcttcttggcaagtacgatgcctcctctatagcacgaaacatagcgaaggaactcggtgggaacggaggaggccgaaagaattttgcccaagcgggtggaagacatccggagaggataaaagacgttctggaacgtctcgaagagtttctgaggtgaAAGCTTccaaa	CATGGcgagatacatgacttctgaagagatcagagaagcctttttgaaattctttgagaaaaagggacacaagatactaccgagcgcttctctcataccggacgatcctcagcttctcttcaccgtagccggtatggttcctttcaaacctattttctggggcaaagtcgagcctgtttacacgagggtggctacctgtcagaagtgtttgagaaccgttgatatagagaacgtgggaaagactccaaggcatcatacgttctttgaaatgctcggtaatttctcctttggagactatttcaaagaagaagccatagagtgggcctgggagttcctcacccaggtgcttggggtccccgaagaaaaactgtgggtctccgtctacgaggaagacgaagaggccttcagaatctggaacgaaaagataggtcttcccgagaagagaattttgagaatgggaaaagaggacaatttctgggggccggctgggccaacagggccatgtggtcccgatacggagatcttttacgatacagggtattcgaagggctgtcctgagggcgaagggtgtactcctgccaattctgaaggacgattcgtggagatatggaaccttgttttcaccgagtattaccaggacgaagaaggaaagctccatcctcttccgagaaagaacatagatacgggagctggtcttgaaaggttttgtgcgatgatgcagggagtttacagcaatttcgacacagatctgttccagccgataataaaacgcatagaggaactgacaggtgttggctacaaaacagatgaagaaaaagatgtttccatcagggtcatagccgatcacataagggccatcacctttttgatcagtgaaggtgttttcccttccaacgagggaagagggtacgttctcagaagaatcataaggcgtgcgatgaggcatggaatactcctcggtatgagtgaaccgttcctctacaggatcgttgatgccgtggtggagaagatgggaaaagtttacccggagatcgttcgtggtgaaggtatggtgaaagaagttctttcagcggaagaaaacagatttctaaagactctcgagcagggaatgaaagtctttgacgagatcgtcgagaagaagggaaaaatagattccgaagacgcgttcagactctacgatacatacggacttccactggagctcacccttgaaatcgcaaaggagaaaggtgtggaagtcgatgtgcaagagttcaacaaatacatggaagaacaacagcgaaagtcaagagctgctatgggcgatgtggaatttgccaggaggtacgagtaccttgaaaaactccccaaagatttccgaaccgaattcactgggtacgaaaaactcgaagatgagggcgaggtcgttctcgtagccagggacgacgaaacggtggaagaagcatcagaaggcacagttgaggtggttttttcaagaactccgttctacgcggagaagggtgggcaggtatccgacacggggatggtggaatggagagatggaaaagcacttgtggaatacgtctttgaagcCtcagaaggtgtgatcgtccacagaataaagatactggatggtactctaagaagaggacagaaagtgattctcagagtggataagaagagaagagaggccacaatgagaaatcacacggcaacccatctgcttcacgcggctttgaagaaagttctgggagaccacgtgaggcaggctgggtccttggttgcacccgacagattgagattcgatttcacacatttcaaaggtctttcgagtgcagaaatagaacaggtggaggatctggtgaacgaatggatcatggaagcgattccggtcgaggtgagatacacgagctacgaagaagcggtaaagtccggagttgttgcgcttttcacggaaaaatacggagacgtggtcagggtcgtcgaggttcccggtgtgagtaaagaactctgtggcggaacgcacgtgaaaaacacagggcagataggtctgttcaagatcatctcggaggaatccgtttcgtcaggtgtgcgaagaatcgaagcggtcaccggcttcagtgctctggaacttctgagaaaccagaaaaagctgatcgatcagctgaaagaaatcctcggggcaagggaagacgagctcacggacagggtcctgagcttgagagaaaaggtgaaggaactcgaaaagaaactctctcagggaaggatttccgaagaaaggatagccatgaaacagctggaagatggtgtaaaggtcttccacggtgtcttcgaaggcgtcgaggcaaaacacctcggaggaatagcagataacgttttgaaaaaagaaggggaaggcattgttattctcttcagtaagttcgaaaacaaggtttccctcgttgtgaaggtttctgagaatcttcttggcaagtacgatgcctcctctatagcacgaaacatagcgaaggaactcggtgggaacggaggaggccgaaagaattttgcccaagcgggtggaagacatccggagaggataaaagacgttctggaacgtctcgaagagtttctgaggtgaA	NcoI/HindIII	1592	1049	2613	insert ala at start	4,2.6	CATGGcgagatacatgacttctgaagagatcagagaagcctttttgaaattctttgagaaaaagggacacaagatactaccgagcgcttctctcataccggacgatcctcagcttctcttcaccgtagccggtatggttcctttcaaacctattttctggggcaaagtcgagcctgtttacacgagggtggctacctgtcagaagtgtttgagaaccgttgatatagagaacgtgggaaagactccaaggcatcatacgttctttgaaatgctcggtaatttctcctttggagactatttcaaagaagaagccatagagtgggcctgggagttcctcacccaggtgcttggggtccccgaagaaaaactgtgggtctccgtctacgaggaagacgaagaggccttcagaatctggaacgaaaagataggtcttcccgagaagagaattttgagaatgggaaaagaggacaatttctgggggccggctgggccaacagggccatgtggtcccgatacggagatcttttacgatacagggtattcgaagggctgtcctgagggcgaagggtgtactcctgccaattctgaaggacgattcgtggagatatggaaccttgttttcaccgagtattaccaggacgaagaaggaaagctccatcctcttccgagaaagaacatagatacgggagctggtcttgaaaggttttgtgcgatgatgcagggagtttacagcaatttcgacacagatctgttccagccgataataaaacgcatagaggaactgacaggtgttggctacaaaacagatgaagaaaaagatgtttccatcagggtcatagccgatcacataagggccatcacctttttgatcagtgaaggtgttttcccttccaacgagggaagagggtacgttctcagaagaatcataaggcgtgcgatgaggcatggaatactcctcggtatgagtgaaccgttcctctacaggatcgttgatgccgtggtggagaagatgggaaaagtttacccggagatcgttcgtggtgaaggtatggtgaaagaagttctttcagcggaagaaaacagatttctaaagactctcgagcagggaatgaaagtctttgacgagatcgtcgagaagaagggaaaaatagattccgaagacgcgttcagactctacgatacatacggacttccactggagctcacccttgaaatcgcaaaggagaaaggtgtggaagtcgatgtgcaagagttcaacaaatacatggaagaacaacagcgaaagtcaagagctgctatgggcgatgtggaatttgccaggaggtacgagtaccttgaaaaactccccaaagatttccgaaccgaattcactgggtacgaaaaactcgaagatgagggcgaggtcgttctcgtagccagggacgacgaaacggtggaagaagcatcagaaggcacagttgaggtggttttttcaagaactccgttctacgcggagaagggtgggcaggtatccgacacggggatggtggaatggagagatggaaaagcacttgtggaatacgtctttgaagcCtcagaaggtgtgatcgtccacagaataaagatactggatggtactctaagaagaggacagaaagtgattctcagagtggataagaagagaagagaggccacaatgagaaatcacacggcaacccatctgcttcacgcggctttgaagaaagttctgggagaccacgtgaggcaggctgggtccttggttgcacccgacagattgagattcgatttcacacatttcaaaggtctttcgagtgcagaaatagaacaggtggaggatctggtgaacgaatggatcatggaagcgattccggtcgaggtgagatacacgagctacgaagaagcggtaaagtccggagttgttgcgcttttcacggaaaaatacggagacgtggtcagggtcgtcgaggttcccggtgtgagtaaagaactctgtggcggaacgcacgtgaaaaacacagggcagataggtctgttcaagatcatctcggaggaatccgtttcgtcaggtgtgcgaagaatcgaagcggtcaccggcttcagtgctctggaacttctgagaaaccagaaaaagctgatcgatcagctgaaagaaatcctcggggcaagggaagacgagctcacggacagggtcctgagcttgagagaaaaggtgaaggaactcgaaaagaaactctctcagggaaggatttccgaagaaaggatagccatgaaacagctggaagatggtgtaaaggtcttccacggtgtcttcgaaggcgtcgaggcaaaacacctcggaggaatagcagataacgttttgaaaaaagaaggggaaggcattgttattctcttcagtaagttcgaaaacaaggtttccctcgttgtgaaggtttctgagaatcttcttggcaagtacgatgcctcctctatagcacgaaacatagcgaaggaactcggtgggaacggaggaggccgaaagaattttgcccaagcgggtggaagacatccggagaggataaaagacgttctggaacgtctcgaagagtttctgaggtgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAGAAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAACAGGAGGAATTAAC
19	pBad myc/hisA		TM-L	leucine	173980..176454	atgaaggaatacagaccacaggaaatagagaaaaaatggcaggaagtatgggaagaaaagaaggtcttctatacacctcagaggtctgagaaacccaagtattatgccctcgtgatgtttccgtatccctctggaactctccacgtagggcacgtgaaaaactacgtcataggtgacatagttgcaagatacaagaggatgagaggatacaacgtccttcacccgttcggatacgacgcattcggacttcccgccgagaacgccgcgatcgaaaagggaattcatcctgaagagtggacaaggaagaacattgccaccattcggcagcaggtgaaaaaactcggtataagctacgactggagcagagaaatagcgacctgcgacgaggagtactacaagtggacccagtggatatttctccagctctacaagaatggtttggcctacaagaaaaaggcggctgtcaactggtgccccaagtgtaaaactgttcttgcgaacgagcaggtgaaagacggaaaatgtgaaagatgtggaacgagcgtcaccataaggcacctcgaacagtggtttttcaagatcacggattacgcggaaagactgctgaacgacctggacaagctcacaggatggccggagcacgtcaagaccatgcagagaaactggataggaaagagcaccggtgcggaaatagattttcctgtcgaaggatcggatacaaagatcagggtcttcacaaccaggcccgacacactctggggtgtcactttcatggctcttgcaccggagtctccccttgttgaagagctcgttcctgaggagaagaaagaagagcttcaggaattccttgaacgtgtaaagcagcaggatagattcagaagaacctctgtcgaagccgagaaagaagggttcttcctgggaaggtacgccataaaccccgtgaccggcgagaggataccgatctacgtggcaaactacatcctcatggagtatggaacgggtgccatcatgggagttcctgcccacgatcagagggacttctccttcgctaaaaaatacggaattccgataaaggttgtgattaaaccagcagacaaagatctcgatccggagaagatggaagaagcctacgaaggcgagggtatcatggtgaactccggaccgttcgatggaacaccgagcagcgaaggaatagaaaaagtcataaattggctcgaggaaaaaggcattggaaagaggtcggtccagtacaagttgagagactggctcatatcgaggcagagatactggggcgctccaatccccatcatctactgtgaaaaatgcggtgttgtacccgttccagaagaggaccttcccgtgagacttccaaaagacgtggaattccttccaacgggtcaatctcctctttctttccacgaaggtttcaagaagacaaagtgccccgtctgtggcggagaagcccagagagaaacggacaccatggacactttcgttgattcttcctggtacttcctcagatacgtgaatcctcacctggaggataagccgttcgagcctgatgacgtgaactactggttaccggtggatcagtacattggaggagtggaacacgccgtactgcatttgctgtactccagattcgtaaccaaggtactgcacgatctgggttatctcaatttcgacgaaccattcaccaaccttttcacacagggaatgatttacaaagacggtgccaagatgtccaagtcgaaggggaacgtggtgtccccggatgagatgatagagaaatacggtgcggataccttgagaatgtacatactcttcatggcacctccagagaaagacgccgaatggagtgacgccggtatcgaaggtgttcacagattcgtcaaaaggctctggaacactttctacacggttcttccgtttgtgaaagaagaaaacacggagaatcttgtgttgaaaaactccacagaaaaggaactcagaagaaagcttcacagtattatcaagaagataacggaggatatagaaggtggattcaagttcaacaccgctataagtggtctgatggaactcgtgaaccacctgagccagtacttgaacagtgtaccgcaggaggaatggaacaggaaactcctgcgagagatagtagagaaactcacactcgctctttcacctttcgcaccgcaccttgcagaggagttctggcacgatctcggaaacgatagtcttgttgtccagcagtcctggccttcctacgatccaaaagcgctcgaagtggaggaagtcgaaatcgccatccagataaatggaaaggtgagagacaaagtggttgtgcctgtggatatctctgaagaggatctgaagaggatcgtcctcgaaagagaaagggtcaaggaatacgtcgatggcaaaccaataaggaaattcatttacgtgaagggaagaatcgtaaatatcgtggtatga	2475	cagtgcGGTCTCccATGGGAaaggaatacagaccacaggaa	tttggAAGCTTtcataccacgatatttacgattcttcc	ggaactcagaagaaaActtcacagtattatc	gataatactgtgaagTtttcttctgagttcc	cagtgcGGTCTCccATGGGAaaggaatacagaccacaggaaatagagaaaaaatggcaggaagtatgggaagaaaagaaggtcttctatacacctcagaggtctgagaaacccaagtattatgccctcgtgatgtttccgtatccctctggaactctccacgtagggcacgtgaaaaactacgtcataggtgacatagttgcaagatacaagaggatgagaggatacaacgtccttcacccgttcggatacgacgcattcggacttcccgccgagaacgccgcgatcgaaaagggaattcatcctgaagagtggacaaggaagaacattgccaccattcggcagcaggtgaaaaaactcggtataagctacgactggagcagagaaatagcgacctgcgacgaggagtactacaagtggacccagtggatatttctccagctctacaagaatggtttggcctacaagaaaaaggcggctgtcaactggtgccccaagtgtaaaactgttcttgcgaacgagcaggtgaaagacggaaaatgtgaaagatgtggaacgagcgtcaccataaggcacctcgaacagtggtttttcaagatcacggattacgcggaaagactgctgaacgacctggacaagctcacaggatggccggagcacgtcaagaccatgcagagaaactggataggaaagagcaccggtgcggaaatagattttcctgtcgaaggatcggatacaaagatcagggtcttcacaaccaggcccgacacactctggggtgtcactttcatggctcttgcaccggagtctccccttgttgaagagctcgttcctgaggagaagaaagaagagcttcaggaattccttgaacgtgtaaagcagcaggatagattcagaagaacctctgtcgaagccgagaaagaagggttcttcctgggaaggtacgccataaaccccgtgaccggcgagaggataccgatctacgtggcaaactacatcctcatggagtatggaacgggtgccatcatgggagttcctgcccacgatcagagggacttctccttcgctaaaaaatacggaattccgataaaggttgtgattaaaccagcagacaaagatctcgatccggagaagatggaagaagcctacgaaggcgagggtatcatggtgaactccggaccgttcgatggaacaccgagcagcgaaggaatagaaaaagtcataaattggctcgaggaaaaaggcattggaaagaggtcggtccagtacaagttgagagactggctcatatcgaggcagagatactggggcgctccaatccccatcatctactgtgaaaaatgcggtgttgtacccgttccagaagaggaccttcccgtgagacttccaaaagacgtggaattccttccaacgggtcaatctcctctttctttccacgaaggtttcaagaagacaaagtgccccgtctgtggcggagaagcccagagagaaacggacaccatggacactttcgttgattcttcctggtacttcctcagatacgtgaatcctcacctggaggataagccgttcgagcctgatgacgtgaactactggttaccggtggatcagtacattggaggagtggaacacgccgtactgcatttgctgtactccagattcgtaaccaaggtactgcacgatctgggttatctcaatttcgacgaaccattcaccaaccttttcacacagggaatgatttacaaagacggtgccaagatgtccaagtcgaaggggaacgtggtgtccccggatgagatgatagagaaatacggtgcggataccttgagaatgtacatactcttcatggcacctccagagaaagacgccgaatggagtgacgccggtatcgaaggtgttcacagattcgtcaaaaggctctggaacactttctacacggttcttccgtttgtgaaagaagaaaacacggagaatcttgtgttgaaaaactccacagaaaaggaactcagaagaaaActtcacagtattatc	ggaactcagaagaaaActtcacagtattatcaagaagataacggaggatatagaaggtggattcaagttcaacaccgctataagtggtctgatggaactcgtgaaccacctgagccagtacttgaacagtgtaccgcaggaggaatggaacaggaaactcctgcgagagatagtagagaaactcacactcgctctttcacctttcgcaccgcaccttgcagaggagttctggcacgatctcggaaacgatagtcttgttgtccagcagtcctggccttcctacgatccaaaagcgctcgaagtggaggaagtcgaaatcgccatccagataaatggaaaggtgagagacaaagtggttgtgcctgtggatatctctgaagaggatctgaagaggatcgtcctcgaaagagaaagggtcaaggaatacgtcgatggcaaaccaataaggaaattcatttacgtgaagggaagaatcgtaaatatcgtggtatgaAAGCTTccaaa	cagtgcGGTCTCccATGGGAaaggaatacagaccacaggaaatagagaaaaaatggcaggaagtatgggaagaaaagaaggtcttctatacacctcagaggtctgagaaacccaagtattatgccctcgtgatgtttccgtatccctctggaactctccacgtagggcacgtgaaaaactacgtcataggtgacatagttgcaagatacaagaggatgagaggatacaacgtccttcacccgttcggatacgacgcattcggacttcccgccgagaacgccgcgatcgaaaagggaattcatcctgaagagtggacaaggaagaacattgccaccattcggcagcaggtgaaaaaactcggtataagctacgactggagcagagaaatagcgacctgcgacgaggagtactacaagtggacccagtggatatttctccagctctacaagaatggtttggcctacaagaaaaaggcggctgtcaactggtgccccaagtgtaaaactgttcttgcgaacgagcaggtgaaagacggaaaatgtgaaagatgtggaacgagcgtcaccataaggcacctcgaacagtggtttttcaagatcacggattacgcggaaagactgctgaacgacctggacaagctcacaggatggccggagcacgtcaagaccatgcagagaaactggataggaaagagcaccggtgcggaaatagattttcctgtcgaaggatcggatacaaagatcagggtcttcacaaccaggcccgacacactctggggtgtcactttcatggctcttgcaccggagtctccccttgttgaagagctcgttcctgaggagaagaaagaagagcttcaggaattccttgaacgtgtaaagcagcaggatagattcagaagaacctctgtcgaagccgagaaagaagggttcttcctgggaaggtacgccataaaccccgtgaccggcgagaggataccgatctacgtggcaaactacatcctcatggagtatggaacgggtgccatcatgggagttcctgcccacgatcagagggacttctccttcgctaaaaaatacggaattccgataaaggttgtgattaaaccagcagacaaagatctcgatccggagaagatggaagaagcctacgaaggcgagggtatcatggtgaactccggaccgttcgatggaacaccgagcagcgaaggaatagaaaaagtcataaattggctcgaggaaaaaggcattggaaagaggtcggtccagtacaagttgagagactggctcatatcgaggcagagatactggggcgctccaatccccatcatctactgtgaaaaatgcggtgttgtacccgttccagaagaggaccttcccgtgagacttccaaaagacgtggaattccttccaacgggtcaatctcctctttctttccacgaaggtttcaagaagacaaagtgccccgtctgtggcggagaagcccagagagaaacggacaccatggacactttcgttgattcttcctggtacttcctcagatacgtgaatcctcacctggaggataagccgttcgagcctgatgacgtgaactactggttaccggtggatcagtacattggaggagtggaacacgccgtactgcatttgctgtactccagattcgtaaccaaggtactgcacgatctgggttatctcaatttcgacgaaccattcaccaaccttttcacacagggaatgatttacaaagacggtgccaagatgtccaagtcgaaggggaacgtggtgtccccggatgagatgatagagaaatacggtgcggataccttgagaatgtacatactcttcatggcacctccagagaaagacgccgaatggagtgacgccggtatcgaaggtgttcacagattcgtcaaaaggctctggaacactttctacacggttcttccgtttgtgaaagaagaaaacacggagaatcttgtgttgaaaaactccacagaaaaggaactcagaagaaaActtcacagtattatcaagaagataacggaggatatagaaggtggattcaagttcaacaccgctataagtggtctgatggaactcgtgaaccacctgagccagtacttgaacagtgtaccgcaggaggaatggaacaggaaactcctgcgagagatagtagagaaactcacactcgctctttcacctttcgcaccgcaccttgcagaggagttctggcacgatctcggaaacgatagtcttgttgtccagcagtcctggccttcctacgatccaaaagcgctcgaagtggaggaagtcgaaatcgccatccagataaatggaaaggtgagagacaaagtggttgtgcctgtggatatctctgaagaggatctgaagaggatcgtcctcgaaagagaaagggtcaaggaatacgtcgatggcaaaccaataaggaaattcatttacgtgaagggaagaatcgtaaatatcgtggtatgaAAGCTTccaaa	cATGGGAaaggaatacagaccacaggaaatagagaaaaaatggcaggaagtatgggaagaaaagaaggtcttctatacacctcagaggtctgagaaacccaagtattatgccctcgtgatgtttccgtatccctctggaactctccacgtagggcacgtgaaaaactacgtcataggtgacatagttgcaagatacaagaggatgagaggatacaacgtccttcacccgttcggatacgacgcattcggacttcccgccgagaacgccgcgatcgaaaagggaattcatcctgaagagtggacaaggaagaacattgccaccattcggcagcaggtgaaaaaactcggtataagctacgactggagcagagaaatagcgacctgcgacgaggagtactacaagtggacccagtggatatttctccagctctacaagaatggtttggcctacaagaaaaaggcggctgtcaactggtgccccaagtgtaaaactgttcttgcgaacgagcaggtgaaagacggaaaatgtgaaagatgtggaacgagcgtcaccataaggcacctcgaacagtggtttttcaagatcacggattacgcggaaagactgctgaacgacctggacaagctcacaggatggccggagcacgtcaagaccatgcagagaaactggataggaaagagcaccggtgcggaaatagattttcctgtcgaaggatcggatacaaagatcagggtcttcacaaccaggcccgacacactctggggtgtcactttcatggctcttgcaccggagtctccccttgttgaagagctcgttcctgaggagaagaaagaagagcttcaggaattccttgaacgtgtaaagcagcaggatagattcagaagaacctctgtcgaagccgagaaagaagggttcttcctgggaaggtacgccataaaccccgtgaccggcgagaggataccgatctacgtggcaaactacatcctcatggagtatggaacgggtgccatcatgggagttcctgcccacgatcagagggacttctccttcgctaaaaaatacggaattccgataaaggttgtgattaaaccagcagacaaagatctcgatccggagaagatggaagaagcctacgaaggcgagggtatcatggtgaactccggaccgttcgatggaacaccgagcagcgaaggaatagaaaaagtcataaattggctcgaggaaaaaggcattggaaagaggtcggtccagtacaagttgagagactggctcatatcgaggcagagatactggggcgctccaatccccatcatctactgtgaaaaatgcggtgttgtacccgttccagaagaggaccttcccgtgagacttccaaaagacgtggaattccttccaacgggtcaatctcctctttctttccacgaaggtttcaagaagacaaagtgccccgtctgtggcggagaagcccagagagaaacggacaccatggacactttcgttgattcttcctggtacttcctcagatacgtgaatcctcacctggaggataagccgttcgagcctgatgacgtgaactactggttaccggtggatcagtacattggaggagtggaacacgccgtactgcatttgctgtactccagattcgtaaccaaggtactgcacgatctgggttatctcaatttcgacgaaccattcaccaaccttttcacacagggaatgatttacaaagacggtgccaagatgtccaagtcgaaggggaacgtggtgtccccggatgagatgatagagaaatacggtgcggataccttgagaatgtacatactcttcatggcacctccagagaaagacgccgaatggagtgacgccggtatcgaaggtgttcacagattcgtcaaaaggctctggaacactttctacacggttcttccgtttgtgaaagaagaaaacacggagaatcttgtgttgaaaaactccacagaaaaggaactcagaagaaaActtcacagtattatcaagaagataacggaggatatagaaggtggattcaagttcaacaccgctataagtggtctgatggaactcgtgaaccacctgagccagtacttgaacagtgtaccgcaggaggaatggaacaggaaactcctgcgagagatagtagagaaactcacactcgctctttcacctttcgcaccgcaccttgcagaggagttctggcacgatctcggaaacgatagtcttgttgtccagcagtcctggccttcctacgatccaaaagcgctcgaagtggaggaagtcgaaatcgccatccagataaatggaaaggtgagagacaaagtggttgtgcctgtggatatctctgaagaggatctgaagaggatcgtcctcgaaagagaaagggtcaaggaatacgtcgatggcaaaccaataaggaaattcatttacgtgaagggaagaatcgtaaatatcgtggtatgaA	BsaI/HindIII	2030	504	2503	internal ncoI site	4,1.5,1	AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACcATGGGAaaggaatacagaccacaggaaatagagaaaaaatggcaggaagtatgggaagaaaagaaggtcttctatacacctcagaggtctgagaaacccaagtattatgccctcgtgatgtttccgtatccctctggaactctccacgtagggcacgtgaaaaactacgtcataggtgacatagttgcaagatacaagaggatgagaggatacaacgtccttcacccgttcggatacgacgcattcggacttcccgccgagaacgccgcgatcgaaaagggaattcatcctgaagagtggacaaggaagaacattgccaccattcggcagcaggtgaaaaaactcggtataagctacgactggagcagagaaatagcgacctgcgacgaggagtactacaagtggacccagtggatatttctccagctctacaagaatggtttggcctacaagaaaaaggcggctgtcaactggtgccccaagtgtaaaactgttcttgcgaacgagcaggtgaaagacggaaaatgtgaaagatgtggaacgagcgtcaccataaggcacctcgaacagtggtttttcaagatcacggattacgcggaaagactgctgaacgacctggacaagctcacaggatggccggagcacgtcaagaccatgcagagaaactggataggaaagagcaccggtgcggaaatagattttcctgtcgaaggatcggatacaaagatcagggtcttcacaaccaggcccgacacactctggggtgtcactttcatggctcttgcaccggagtctccccttgttgaagagctcgttcctgaggagaagaaagaagagcttcaggaattccttgaacgtgtaaagcagcaggatagattcagaagaacctctgtcgaagccgagaaagaagggttcttcctgggaaggtacgccataaaccccgtgaccggcgagaggataccgatctacgtggcaaactacatcctcatggagtatggaacgggtgccatcatgggagttcctgcccacgatcagagggacttctccttcgctaaaaaatacggaattccgataaaggttgtgattaaaccagcagacaaagatctcgatccggagaagatggaagaagcctacgaaggcgagggtatcatggtgaactccggaccgttcgatggaacaccgagcagcgaaggaatagaaaaagtcataaattggctcgaggaaaaaggcattggaaagaggtcggtccagtacaagttgagagactggctcatatcgaggcagagatactggggcgctccaatccccatcatctactgtgaaaaatgcggtgttgtacccgttccagaagaggaccttcccgtgagacttccaaaagacgtggaattccttccaacgggtcaatctcctctttctttccacgaaggtttcaagaagacaaagtgccccgtctgtggcggagaagcccagagagaaacggacaccatggacactttcgttgattcttcctggtacttcctcagatacgtgaatcctcacctggaggataagccgttcgagcctgatgacgtgaactactggttaccggtggatcagtacattggaggagtggaacacgccgtactgcatttgctgtactccagattcgtaaccaaggtactgcacgatctgggttatctcaatttcgacgaaccattcaccaaccttttcacacagggaatgatttacaaagacggtgccaagatgtccaagtcgaaggggaacgtggtgtccccggatgagatgatagagaaatacggtgcggataccttgagaatgtacatactcttcatggcacctccagagaaagacgccgaatggagtgacgccggtatcgaaggtgttcacagattcgtcaaaaggctctggaacactttctacacggttcttccgtttgtgaaagaagaaaacacggagaatcttgtgttgaaaaactccacagaaaaggaactcagaagaaaActtcacagtattatcaagaagataacggaggatatagaaggtggattcaagttcaacaccgctataagtggtctgatggaactcgtgaaccacctgagccagtacttgaacagtgtaccgcaggaggaatggaacaggaaactcctgcgagagatagtagagaaactcacactcgctctttcacctttcgcaccgcaccttgcagaggagttctggcacgatctcggaaacgatagtcttgttgtccagcagtcctggccttcctacgatccaaaagcgctcgaagtggaggaagtcgaaatcgccatccagataaatggaaaggtgagagacaaagtggttgtgcctgtggatatctctgaagaggatctgaagaggatcgtcctcgaaagagaaagggtcaaggaatacgtcgatggcaaaccaataaggaaattcatttacgtgaagggaagaatcgtaaatatcgtggtatgaAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG
20
21
22	oligos
23	name	sequence
24	oligo-F-Cys	ccaaaCCATGGcgagaataaccaacaccctgacagg
25	oligo-F-Asp	ccaaaCCATGGccctgagaacacacacgtgtggagagc
26	oligo-F-Glu	ccaaaCCATGGgcttcatcaccggggctttttttg
27	oligo-F-Phe	ccaaaCCATGGagatagaagccgtcgaa
28	oligo-F-Gly	ccaaaCCATGGcgtatcttcaggatgtgataatgaaac
29	oligo-F-His	ccaaaCCATGGcgaaatacagaaggataaagggaaca
30	oligo-F-Ile	cagtgcGGTCTCccatggaatacaaaaacacactgaac
31	oiligo-F-Lys	ccaaaCCATGGcgctgaaagagttcaaagaacaacgac
32	oligo-F-Glu(2)	ccaaaCGTCTCccatggtaagggtgagattcgcaccg
33	oligo-F1-Ala	ccaaaCCATGGcgagatacatgacttctgaagagatc
34	oligo-F2-Ala	tacgtctttgaagcCtcagaaggtgtga
35	oligo-F1-Leu	cagtgcGGTCTCccATGGGAaaggaatacagaccacaggaa
36	oligo-F2-Leu	ggaactcagaagaaaActtcacagtattatc
37	oligo-R-Cys	tttggAAGCTTtcactcgacagtgtattctgtccc
38	oligo-R-Asp	tttggAAGCTTttatccctcctctattctaattctg
39	oligo-R-Glu	tttggAAGCTTttaggttttctttaagaactgtaaag
40	oligo-R-Phe	tttggAAGCTTtcagaatctccttcttattcca
41	oligo-R-Gly	tttggAAGCTTctatacctgagatctcttgac
42	oligo-R-His	tttggAAGCTTtcaatcttttgaaaccctttcagcg
43	oligo-R-Ile	tttggAAGCTTtcacttcctttctcctttcaaaac
44	oiligo-R-Lys	tttggAAGCTTtcattcttcaaaccctccttcgaat
45	oligo-R-Glu(2)	tttggAAGCTTtcatccccccagagatctttctattct
46	oligo-R1-Ala	tttggAAGCTTtcacctcagaaactcttcgagacg
47	oligo-R2-Ala	tcacaccttctgaGgcttcaaagacgta
48	oligo-R1-Leu	tttggAAGCTTtcataccacgatatttacgattcttcc
49	oligo-R2-Leu	gataatactgtgaagTtttcttctgagttcc
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