ABCD
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Calculating distance from FRET efficiency
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Type the Förster distance R0 and the measured FRET-eff. E(%) into the yellow cells, the fluorophore distance is calculated
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CFP/YFP-Förster distance: 5 nm
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Förster distanceR05nm
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FRET efficiencyE (%)10%
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Fluorophore distancer7.211247852nm
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