Name of Institution or University

Conference Presentation for Cell Signaling and Molecular Biology Research

Your name here

Your title or role here

Name of conference or event

Date of conference

Name of Institution or University

Conference Presentation for Cell Signaling and Molecular Biology Research

Your name here

Your title or role here

Name of conference or event

Date of conference

Want a different color palette? One change recolors the whole deck.

PowerPoint — Design → Variants → Colors → Customize Colors. Fonts: Design → Variants → Fonts.

Google Slides — Slide → Edit theme → Colors.

Need more detail? Read the full recoloring guide.

Delete this slide when you are done.

Table of Contents

I

Research background

II

Hypothesis and objectives

III

Methodology

IV

Results

V

Discussion and next steps

Funding and Disclosures

1

Funding sources — National Institutes of Health (NIH), Grant No. XYZ12345; National Science Foundation (NSF), Grant No. ABC67890

2

Conflicts of interest — Dr. A. Researcher holds a patent related to the work presented. No other conflicts of interest to declare.

3

Ethics approval — Human studies approved by the Example University IRB, Protocol 2026-XYZ; animal studies by the IACUC, Protocol 2026-ABC.

4

Data availability — Sequencing data deposited under accession GSE000000; analysis code archived at doi.org/10.0000/example

I · RESEARCH BACKGROUND

Introduction

Signaling pathways translate extracellular cues into transcriptional programs, and a handful of them account for most of the decisions a cell makes.

The JAK-STAT axis is central to cytokine response and cell fate, and its dysregulation is implicated in both inflammation and malignancy.

Give only the background needed to follow what comes next.

Need a diagram like this? Describe it and create one in seconds at SciFig.

I · RESEARCH BACKGROUND

One Figure, One Message

Drop your key figure here.

One slide, one message — if it needs two, make two slides.

No figure fits your data? Describe it and create one in seconds at SciFig.

I · RESEARCH BACKGROUND

Knowledge Gap and Open Questions

What is not yet known

State it plainly. For example, "It is unclear whether the effect is driven by the receptor itself or by the adaptor it recruits."

Why the gap has persisted. For example, "The two have never been separable in a live-cell assay."

What closing it would change. For example, "It would tell us which of the two is the druggable node."

Questions this work asks

Can the two contributions be separated in a living cell?

Does the effect survive when the adaptor alone is removed?

Which of the two tracks with the phenotype across conditions?

II · HYPOTHESIS AND OBJECTIVES

The Testable Claim

We hypothesize that loss of the pathway's negative regulator sustains STAT activation and drives the observed phenotype.

The same shape, blank: "We hypothesize that [independent variable] will [expected effect] on [dependent variable] in [population or condition]."

Testable by rescuing the regulator and measuring pathway output.

II · HYPOTHESIS AND OBJECTIVES

Research Objectives

1

What you set out to measure. For example, "To quantify how knockout of the regulator changes pathway output over 24 hours."

2

What you set out to compare. For example, "To compare the knockout against a wild-type rescue as a specificity control."

3

What you set out to validate. For example, "To confirm the phenotype in a second, independent cell line."

4

What you set out to replicate. For example, "To repeat the time-course with an independent guide RNA."

III · METHODOLOGY

Study Design and Methods

Design and sample size. For example, "CRISPR-Cas9 knockout in a stable line, two independent guides, three biological replicates each."

Key technique and why. For example, "Wild-type rescue as the specificity control — a single-guide knockout alone cannot rule out off-target effects."

Readout and statistics. For example, "Immunoblot, qPCR and single-cell RNA-seq; significance at alpha = 0.05."

Need a diagram like this? Describe it and create one in seconds at SciFig.

III · METHODOLOGY

Experiment Timeline

Phase 1 — setup and validation. For example, "Months 1-3: line generation, guide validation, and assay calibration."

Phase 2 — main data collection. For example, "Months 4-9: full time-course across both guides, three replicates each."

Phase 3 — replication and analysis. For example, "Months 10-12: second cell line and final analysis."

Need a diagram like this? Describe it and create one in seconds at SciFig.

IV · RESULTS

Result 1 — For Example, "Knockout Sustains Pathway Activation Past 12 Hours"

Lead with what you found in plain language, then point at the part of the figure that shows it.

Give the effect size and its uncertainty, not just the p-value. For example, "2.4-fold higher at 12 h (95% CI 1.8-3.1)."

Title this slide with the finding rather than with "Result 1" — a title that states the result does half the talking for you.

Describe this figure in one line: what is plotted, and what it shows.

IV · RESULTS

Result 2 — For Example, "The Effect Scales With Baseline Expression"

What changed between conditions. For example, "The same trend held in the replication line, with a smaller slope."

Any result that went against your expectation — say it here. For example, "The rescue only partially reversed the phenotype."

Say what the axes are before you say what they mean.

Describe this figure in one line: what is plotted, and what it shows.

IV · RESULTS

Summary of Findings

V · DISCUSSION AND NEXT STEPS

What the Results Mean

What it means for the field. For example, "This places the regulator upstream of the branch point in the known pathway map."

How it fits or clashes with prior work. For example, "It disagrees with the published knockout phenotype, which was measured in a different cell background."

The limitation a reviewer raises first. For example, "Single cell line, in vitro only — no primary tissue yet."

Need a diagram like this? Describe it and create one in seconds at SciFig.

V · DISCUSSION AND NEXT STEPS

Troubleshooting

What is not working

What broke, and what you already tried. For example, "The assay drifts after passage 12; fresh thaws and recalibration did not fix it."

The failure mode you suspect. For example, "Clonal drift, not reagent variability — it tracks with passage number, not lot."

How far it sets the timeline back.

What would unblock it

A second pair of hands for the passage-matched repeat.

An assay that does not depend on late-passage cells.

Access to the earlier frozen stock, if it still exists.

V · DISCUSSION AND NEXT STEPS

Remaining Questions and Next Steps

Questions still open

The question this work opened up. For example, "Does the partial rescue mean a second regulator is involved?"

The result that would change the interpretation if it did not hold.

The assumption the whole model still rests on.

What comes next

The experiment that would answer it. For example, "A double knockout with the candidate regulator, on the same time-course."

What it would take to run. For example, "Two months of scope time and access to the primary tissue bank."

What would have to be true before scaling this up.

References

1. Author, A. A., Author, B. B., & Author, C. C. (Year). Title of the article. Title of the Journal, volume(issue), page range. https://doi.org/10.0000/example

2. Author, D. D., & Author, E. E. (Year). Title of the article. Title of the Journal, volume(issue), page range. https://doi.org/10.0000/example

3. Author, F. F., et al. (Year). Title of the article. Title of the Journal, volume(issue), page range. https://doi.org/10.0000/example

4. Author, G. G., & Author, H. H. (Year). Title of the preprint. Preprint server. https://doi.org/10.0000/example

5. Author, I. I., et al. (Year). Title of the dataset [Data set]. Repository. https://doi.org/10.0000/example

6. Author, J. J., & Author, K. K. (Year). Title of the chapter. In Editor (Ed.), Title of the book (pp. 000-000). Publisher.

7. Author, L. L., et al. (Year). Title of the article. Title of the Journal, volume(issue), page range. https://doi.org/10.0000/example

THANK YOU

Acknowledgments

Lab or department

Name — principal investigator

Name — co-first author

Name — sequencing and analysis

Name — animal work

Name — imaging support

Name

Collaborators and funding

Name — collaborating lab

Name — statistics

Name — clinical samples

NIH Grant No. XYZ12345

NSF Grant No. ABC67890

your.email@institution.edu

Name of Institution or University

QR code to your paper, preprint, dataset or lab page

Make a QR code free

Insert a picture of your team here

Supplementary Resources

The next slides hold ready-made figures and icons you can drop straight into this deck — and a link to the rest.

Resources: Lab Equipment and Consumables

Use these resources to level up your presentation

96-well plate

Zoomed callout

Coplin jar

Lab mouse

Magnetic cell separator

Imaging flow cytometer

Petri dish

Cubitainer

Browse free scientific icons at SciFig — or generate any figure free.

Resources: Data and Analysis Figures

Use these resources to level up your presentation

Action potential

GC-MS schematic

PRISMA flow diagram

Flow cytometry plot

Heat map

Kaplan-Meier curve

Browse free figure templates at SciFig — or generate any figure free.

Resources: Cell Signaling and Pathway Figures

Use these resources to level up your presentation

Browse the full figure gallery at SciFig — or generate any figure free.