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Protein Purification:

Wrapping up 4.1

March 7th, 2023

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Welcome and Objectives

Thank you for taking the time to be here today!

Objectives:

  • Complete a column chromatography
  • Gain confidence preparing and completing the lab in 4.1.3
  • Collaborate with other MI teachers

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Agenda

  • Introductions
  • Before the Lab
    • Directions
    • Teacher prep
  • During the Lab
    • Column chromatography
      • Set up
      • Completion
    • 3D Molecular Designs - Amino Acid Starter Kit
  • Time to Plan/Reflect
  • Debrief/Wrap Up

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Danielle Weber

  • School/City
    • Academy of Information Technology and Engineering; Stamford, CT

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  • PLTW Info
    • Currently Teach: HBS and MI
    • Previously Taught: PBS and BI
    • Master Teacher: HBS and MI
    • 2021-2022 Biomed Teacher of the Year

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Before the Lab

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4.1.1 - All About Insulin

Students research the history of treating diabetes and how insulin has been obtained

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4.1.2 - Protein Factories

Students complete bacterial transformation

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4.1.3 - Protein Purification

Edvotek 225

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Ordering

Storage

Teacher Prep

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During the Lab

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Making your Column

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Running Your Column

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4.1.4 - Protein Electrophoresis

Students will run a protein gel following the collection of their purified protein samples

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Building Proteins

Follow student directions

Work with models

Identify student misconceptions

and possible differentiation

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Time to Plan

How will you complete 4.1.3?

What is your timeline?

What support do you need to make this happen?

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Contact Info

Danielle Weber

  • weber.danielle.l@gmail.com
  • 740.405.7701