AQ curious about
Promoters?
Joshua Baker, Haley Everroad, and Madison Powell
Introduction
The Main Question
DY Wu et al. Nature 462, 1056-1060 (2009) doi:10.1038/nature08656
Actinobacteria
Gammaproteobacteria
Take away:
E. coli and Arthrobacter are not very related. Are the promoter sequences conserved?
MEthods
Results
Average Gap Size Between Genes
10 & 11: 17.64285714
Average Gap Size Between Genes
83 & 84:
17.48484848
| 1 | 2 | 3 | 4 | 5 | 6 |
Gene 10/11 -10 Promoter | C/A | A | T | A | T/A | T |
Gene 83/84 -10 Promoter | T | A | T | A | A/T | T |
-10 Promoter Consensus | T | A | T | A | A/T | T |
Gene 10/11 -35 Promoter | T/G | T | G | A | A/G | A |
Gene 83/84 -35 Promoter | T | T | G | A/C | C/T/A | A |
-35 Promoter Consensus | T | T | G | A | A/C | A |
Results
| 1 | 2 | 3 | 4 | 5 | 6 |
-10 CONSENSUS | T | A | T | A | A/T | T |
-35 CONSENSUS | T | T | G | A | A/C | A |
| 1 | 2 | 3 | 4 | 5 | 6 |
-10 CONSENSUS | T | T | G | A | T | T |
-35 CONSENSUS | T | T | G | A | C | G |
| 1 | 2 | 3 | 4 | 5 | 6 |
-10 CONSENSUS | T | A | T | A | A | T |
-35 CONSENSUS | T | T | G | A | C | A |
AQ PHAGES
AVERAGE GAP:
17.56385281
AN PHAGES
AVERAGE GAP:
16.82692308
E. coli
AVERAGE GAP:
17
Rho - Independent Transcription Terminase Prediction
Gene 10 & 11 Promoter Verification
Predicted Promoter Region
Reverse Strand Predicted Terminase (1364 bp)
Score: -16.10
Predicted Protein Coding Sequence
Forward Strand Predicted Terminase (32151 bp) Score: -16.40
Gene 83 & 84 Promoter Verification
Predicted Promoter Region
Predicted Protein Coding Sequence
Reverse Strand Predicted Terminase (47445 bp)
Score: -17.60
Forward Strand Predicted Terminase (32151 bp) Score: -16.40
10/11 Reverse Strand Termination Sequence
10/11 Forward Strand Termination Sequence
83/84 Reverse Strand Termination Sequence
83/84 Forward Strand Termination Sequence
Discussion
Conclusion
REFERENCES
ACKNOWLEDGEMENTS
Questions?