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1

Laporan Bulanan November 2020 Evi Sulastri

Referensi :

  1. Alves, A. et al. (2010) ‘Extraction and physico-chemical characterization of a versatile biodegradable polysaccharide obtained from green algae’, Carbohydrate Research. Elsevier Ltd, 345(15), pp. 2194–2200. doi: 10.1016/j.carres.2010.07.039.
  2. Costa, C. et al. (2012) ‘Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure’, Carbohydrate Polymers. Elsevier Ltd., 88(2), pp. 537–546. doi: 10.1016/j.carbpol.2011.12.041.
  3. Quemener, B.; Lahaye, M.; Bobin-Dubigeon, C. Sugar Determination in Ulvans by a Chemical-Enzymatic Method Coupled to High Performance Anion Exchange Chromatography. J. Appl. Phycol. 1997, 9 (2), 179–188. https://doi.org/10.1023/A:1007971023478.

​

Protokol :

  • Sampel dihidrolisis menggunakan asam trifluoroasetat 2 M.

Daftar isi

  1. Analisa komposisi monosakarida menggunakan HPLC

Title : Pengujian kadar sulfat

Purpose : Untuk menentukan komposisi monosakarida sampel UC, Up1 dan UP2

Title : PENGEMBANGAN FILM HIDROGEL BERBASIS POLISAKARIDA ULVAN DARI ALGA HIJAU (Ulva lactuca Linn.) UNTUK PENGOBATAN LUKA BAKAR DERAJAT DUA

Purpose : Ekstraksi, produksi sediaan dan uji aktivitas film hidrogel ulvan dari alga hijau Ulva lactuca

Parameter

UC

UP1

UP2

Glucuronic Acid (%)

-

21

15

Xylose (%)

-

6

0.86

Monosaccharides composition of Ulvan from Ulva lactuca Linn.

Hasil

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Laporan Bulanan November 2020 Evi Sulastri

Referensi :

  1. Li, P.; Wen, S.; Sun, K.; Zhao, Y.; Chen, Y. Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix Flacca. 2018. https://doi.org/10.3390/md16080281.
  2. Costa, C. et al. (2012) ‘Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure’, Carbohydrate Polymers. Elsevier Ltd., 88(2), pp. 537–546. doi: 10.1016/j.carbpol.2011.12.041.

​

Protokol :

  • 1H and 13C NMR spectra were recorded at 18oC using JEOL , the pre-dried polysaccharide sample dissolved in D2O

Daftar isi

  1. Analisa NMR

Title : Analisa NMR

Purpose : Untuk menentukan struktur

Title : PENGEMBANGAN FILM HIDROGEL BERBASIS POLISAKARIDA ULVAN DARI ALGA HIJAU (Ulva lactuca Linn.) UNTUK PENGOBATAN LUKA BAKAR DERAJAT DUA

Purpose : Ekstraksi, produksi sediaan dan uji aktivitas film hidrogel ulvan dari alga hijau Ulva lactuca

Hasil

13C NMR spectra of UP2 recorded at 18oC (D2O)

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1H and 13C NMR chemical shift assignment for the main sugar units of UP2

Residues

C1/H1

C2/H2

C3/H3

C4/H4

C5/H5

C6/H6

A (1 4)-α-L-Rha

101.392

68.996

70.426

79.046

71.463

17.048

5.053

4.011

4.699

3.435

3.703

1.258

B (1 4)-α-L-Rha3S

100.106

68.353

78.931

76.147

68.209

16.799

4.800

4.192

4.556

3.303

3.935

1.173

C (1 4)-β-D-GlcA

​

103.321

73.940

75.984

78.211

-

175.715

4.797

3.398

3.532

3.607

-

-

​

​

1H NMR spectrum of UP2 recorded at 18oC (D2O)

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Timeline Kemajuan Penelitian (Semester 3)

No

Kegiatan

Waktu (Bulan)

Keterangan

Ekstraksi & Karakterisasi Ulvan

Tempat

1

Produksi Crude Ulvan

Lab. Penelitian Univ. Tadulako

​

Juli 2020

Telah dilakukan

2

Purifikasi Ulvan

Lab. Penelitian Univ. Tadulako

Agustus 2020

Telah dilakukan

3

Analisa komposisi Crude Ulvan dan Ulvan terpurifikasi:

  • Yield, kadar sulfat, protein, total karbohidrat, lipid, kadar air, kadar abu

Lab. Penelitian Univ. Tadulako

Agustus 2020

Telah dilakukan

4

Karakterisasi Ulvan

  • Analisa FTIR

UNHAS

September 2020

Telah dilakukan

5

  • Analisis NMR
  • Analisa monosakarida

LIPI

Oktober 2020

Telah dilakukan

6

  • Pengukuran viskositas dan sifat alir
  • Pengujian tekstur

LIPI

November 2020

Masih berlangsung

7

Persiapan seminar kemajuan 2

Farmasi Unpad

November 2020

Desember 2020