• Rules: Number 1 (The Artist) – Draw what you can from the description without turning around
• Number 2 (The Narrator) - Describe what you can see without using your hands

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B4 Enzymes

Draw what your partner describes to you.

B4.1 Define enzymes as proteins that function as biological catalysts

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What is this lesson about?

Do Now

https://www.youtube.com/watch?v=rlH1ym916Fo

B4 Enzymes

Draw what your partner describes to you.

B4.1 Define enzymes as proteins that function as biological catalysts

Products

Catalyst

Denatured

Optimal

Collisions

Kinetic

• An enzyme is a biological c________________ made of _________. This means it speeds up/slows down reactions. 

(circle the correct answer)  

• A specific substrate f____________ into the a___________ site of an e____________________. Find an image of this and insert it below 

• The enzyme is the same as a l________________ and the substrate acts as a key. 

• If the enzyme’s active site changed shape, the substrate could/couldn’t fit into the enzyme. The enzyme has become d____________________. Find an image of a denatured enzyme and insert it below: 

• Enzymes have an o___________________ temperature and pH. As the temperature increases, there are more ____________ due to increased _____________ energy. Find a graph which shows this and insert it below 

catalyst

protein

fits

active

enzyme

lock

denatured

optimum

collisions

kinetic

• Digestive enzymes are the chemicals that break large insoluble food molecules into smaller soluble molecules.

B4. Enzymes

How do digestive enzymes help the process of digestion?

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Enzymes

How are Proteins digested?

PROTEASE

Large Molecule

Small Molecule

Protein

Protein

Amino Acids

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B4. Enzymes

Produced in the stomach, pancreas and the small intestine

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Enzymes

Task 2:

Protease

Amino Acids

B4. Enzymes

Fill in the table using the information

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Carbohydrase

(AMYLASE)

Large Molecule

Small Molecule

Carbohydrate

Glucose

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B4. Enzymes

Produced in the salivary glands, pancreas and the small intestine

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Protease

Amino Acids

Carbohydrate

https://www.twig-world.com/film/glossary/amylase-239/

Amylase

Glucose

B4. Enzymes

Fill in the table using the information

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

LIPASE

Large Molecule

Small Molecule

Lipid

B4. Enzymes

Produced in the pancreas and the small intestine

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Protease

Amino Acids

Carbohydrate

https://www.twig-world.com/film/glossary/amylase-239/

Amylase

Glucose

Lipids

Lipase

B4. Enzymes

Fill in the table using the information

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

B4 Enzymes

Explain using the word bank

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

https://quizlet.com/_8lz085?x=1jqt&i=28b19k

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B4 Enzymes

Complete the missing words

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

B4 Enzymes

Challenge: What does this image mean?

B4.2 Explain enzyme action with reference to the complementary shape of the active site of an enzyme and its substrate and the formation of a product

Carbohydrase (Amylase)

Starch (10,000 glucose joined together)

glucose

molecules

Enzymes

How do digestive enzymes help the process of digestion?

Checkpoint

protease

protein

amino acids

Enzymes

How do digestive enzymes help the process of digestion?

Checkpoint

bile

lipase

+

fat molecule

glycerol

fatty acids

B4 Enzymes

How do digestive enzymes help the process of digestion?

Checkpoint

3. Types of Enzymes.

2. Places that all enzymes are produced.

1. Definition for an enzyme.

B4 Enzymes

How do digestive enzymes help the process of digestion?

Checkpoint

Salivary Glands

Amylase

long carbohydrate molecule (starch)

glucose

molecules

B4 Animal Nutrition

Salivary Amylase vs Starch Investigation

• Number 1

Collect small beaker

Pipette

Test Tube

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• Number 2

Collect 2 x spotting tile

Iodine

Stopwatch

• Number 3

Measure 15ml of Starch

Using measuring cylinder

Enzyme Practical

Nominate a number 1,2, or 3

Task 1

If there is only 2 of you, Number 1 can do both 1 and 2

Next….

Role 1: Spitter – spit your saliva into the small beaker

Role 2: Spotter – drop one drop of iodine into 24 dimples

Role 3: Setter – setup everything ready to go

1. Mix the saliva and starch for 5 seconds
2. Drop 2 drops of the mixture into the first dimple using a pipette
3. Start the stopwatch
4. Stir the mixture for 30 seconds (do not stop the stopwatch)
5. Drop 2 drops of the mixture into the second dimple using a pipette
6. Continue steps 4 & 5 until the dimple remains brown.

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Enzyme Practical

Follow the method

Task 1

bile

Enzymes

What is bile?

Extra Information

Bile is secreted into the small intestine where it has two effects:

1. Neutralises the acid - providing the alkaline conditions needed in the small intestine.
2. Emulsifies fats - providing a larger surface area over which the lipase enzymes can work

B4 Enzymes

Who will be the Kahoot Champion?

Checkpoint

https://create.kahoot.it/share/enzymes/65cb415c-957b-45ee-981b-a19f40ee9b90

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Enzymes

Task 2: State where each enzyme is produced

Stomach

Pancreas

Small Intestine

Salivary Glands

• 1 x small beaker of amylase
• 1 x small beaker of Carbohydrate (starch)
• 1 x thermometer
• 1 x large beaker
• 2 x pipettes
• 2 x test tubes
• 1 x thermometer
• 1 x stopwatch
• 1 x iodine
• 1 x spotting tile

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Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

Collect the following and place in your tray

• Part 1: Preparing the water bath
• Collect materials listed
• Fill the LARGE beaker with approximately 100ml of hot water
• Measure the temperature of the water (between 20-30C)
• Record the temperature below.

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Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

Collect the following and place in your tray

• Part 2: Preparing Solutions
• Measure 2ml of amylase using a pipette
• Put the 2ml of amylase into a test tube
• Place the test tube in the water bath
• Measure 6ml of starch using a pipette
• Put the 6ml of starch into a test tube
• Place the test tube in the water bath
• Start the stopwatch leave for 2 minutes
• Add 1 drop of iodine to each dimple of the spotting tile whilst you are waiting

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Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

• Part 3: Testing
• Add the starch to the amylase test tube
• Stir for approximately 10 seconds
• Take a small sample of the mix using a pipette
• Squeeze the sample into the first dimple
• Start the stopwatch
• Repeat every 30 seconds (without stopping the stopwatch) until the spotting tile is full.

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Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

Fill in the empty time boxes for every 30 seconds

Enzymes

Task

Investigating the effect of temperature on rate of enzyme action

Blue-Black

Orange-Brown

Carbohydrate

Carbohydrase

Glucose

Carbohydrase

Carbohydrate

Orange-Brown