1 of 11

2 of 11

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

3 of 11

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Reaction temp

37°C

37°C 47°C 55°C

37°C 55°C

37°C 47°C 55°C

Lane ID

dNTP

—

WT TdT

47°C

dCTP

dATP

dTTP

dGTP

4 of 11

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Reaction temp

37°C

37°C 47°C 55°C

Lane ID

dCTP

dATP

dTTP

dGTP

dNTP

—

ThTdT

5 of 11

Primer

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

dCTP

dATP

dTTP

dGTP

Wild type TdT: Addition of each type of nucleotide over a range of temperatures (37, 47, 55°C)

dTTP

37°C

no dNTP

6 of 11

Primer

dCTP

dATP

dTTP

dGTP

Wild type TdT: Addition of each type of nucleotide over a range of temperatures (37, 47, 55°C)

no dNTP

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

7 of 11

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

dCTP

dATP

dTTP

dGTP

ThTdT: Addition of each type of nucleotide over a range of temperatures (37, 47, 55°C)

Primer

37°C

no dNTP

8 of 11

9 of 11

10 of 11

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

dCTP

dATP

dTTP

dGTP

Primer

no dNTP

37°C

Wild type TdT: Addition of each type of nucleotide over a range of temperatures (37, 47, 55°C)

11 of 11

Primer

no dNTP

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

37°C

47°C

55°C

dCTP

dATP

dTTP

dGTP

ThTdT: Addition of each type of nucleotide over a range of temperatures (37, 47, 55°C)