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Genome-Wide Identification and Characterization of Gene Family

DR MUHAMMAD SHAFIQ

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Introduction

  • • Importance of organism
  • • Overview of gene family
  • • Biological significance
  • • Knowledge gap

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Objectives

  • • Identify gene family members
  • • Structural and phylogenetic analysis
  • • Expression profiling
  • • Functional prediction

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Data Sources

  • • Genome database (NCBI/Ensembl)
  • • Protein & CDS sequences
  • • Pfam/SMART
  • • GEO/RNA-seq

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Methodology Workflow

  • • Genome retrieval
  • • BLAST/HMM search
  • • Domain confirmation
  • • Phylogeny & motif analysis

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Identification of Gene Family

  • • Total genes identified
  • • Protein length, MW, pI
  • • Gene naming

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Phylogenetic Analysis

  • • Tree construction method
  • • Subgroup classification
  • • Evolutionary insights

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Gene Structure & Motifs

  • • Exon–intron organization
  • • Conserved motifs
  • • Subgroup conservation

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Chromosomal Distribution

  • • Gene location on chromosomes
  • • Tandem duplication

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Duplication & Synteny

  • • Segmental duplication
  • • Evolutionary expansion
  • • Ka/Ks analysis

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Expression Analysis

  • • Tissue-specific expression
  • • Stress/hormone response
  • • Heatmap visualization

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Discussion

  • • Functional implications
  • • Comparison with previous studies

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Conclusion

  • • Key findings
  • • Biological significance
  • • Research contribution

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Future Perspectives

  • • Functional validation
  • • Gene editing
  • • Breeding applications

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Acknowledgements

  • • Supervisor
  • • Funding
  • • Lab members

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References

  • • Key journal articles