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LeviCell EOS Commercial Release Training

06/26/2023

Confidential – For Internal Use Only

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What to Expect w/ the

Commercial Release of LeviCell EOS

3

2

1

Closer Look at LeviCell EOS

Support Plan

Agenda

Confidential – For Internal Use Only

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What to Expect with the

Commercial Release of LeviCell EOS

Confidential – For Internal Use Only

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Now Available - LeviCell EOS

SCALE throughput & connected modules

FLEXIBILITY temperature & applications

PROVEN Levitation Technology

VISUALIZE sample characterization

Confidential – For Internal Use Only

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Unprecedented Parallel Processing Power

Enabling ultimate flexibility & capability to scale sample throughput

​

​

​

1

2

3

4

Control & Configure Independently

4x4

Throughput

PC

Central Hub

Confidential – For Internal Use Only

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Congratulations to the Lexi R&D Team & Extended Team!

It’s been a long time but we made it!

Confidential – For Internal Use Only

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EOS Pre-Order Funnel

43 systems in

Evaluating, Financing, Purchasing or

Closed won

APAC

EU

AMER

Confidential – For Internal Use Only

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LeviCell EOS Commercial Release Activities

June 2023

Ship EOS

6/30/2023

Commercial Refresh Training

Collateral Release

  • Product webpage updates
  • Sales flyer update
  • Software Demo Tool
  • Expanded Internal FAQ
  • User Guide
  • Viable Cell Enrichment QRG
  • Bead Test QRG
  • Order to Training Checklist
  • Installation Record

July 2023

Aug 2023

1st, 2nd Installs

​

​

​

​

​

​

​

​

​

​

​

​

​

​

​

Instrument Pre-check by R&D

Before releasing into inventory

Collateral Release

  • Instrument Family Brochure
  • TF: LeviSelect EOS
  • Webinar summary
  • TF: Visualization on EOS

3rd Install

Install

Boston Demo Site

Marketing Communication Activities

Price change

No trade ins

Confidential – For Internal Use Only

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Marketing & Support Material

User Guide

Sales Flyer

Viable Cell Enrichment QRG

Bead Test QRG

EOS Manager

GUI Demo and Guide

Confidential – For Internal Use Only

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Support Documents

Site Prep

Delivery to Training Checklist

Updated FAQ

Installation Record

Confidential – For Internal Use Only

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Marketing Communications: LeviCell EOS Launch

11

1

2

3

4

AdWords

Campaign

​

​

Weekly emails

Social Media 2x/week

Digital Web updates/

Levisphere news

5

New collateral

6

Events

​

TARGET AUDIENCE

COMMUNICATIONS PLAN

HUBSPOT CONTACTS

(Names, leads, MQLs, SQLs)

​

​

TARGETS:

  • Single cell applications

​

​

APPROXIMATE TOTAL:

  • 33,000

DELIVERABLES

  • ADWORDS CAMPAIGN
    • 3PPC ads; 3 display ads across 30 targeted websites
  • 9 EMAIL COMMS
  • 20 SOCIAL MEDIA POSTS
  • WEBSITE UPDATES
    • All pages including EOS, Levitation
  • LEVISPHERE LAUNCH - Launch of monthly online newsletter
  • NEW COLLATERAL
    • Technical Note (LeviCell vs. EOS)
    • RS: Rescue Your Cells
    • Webinar summary (GEN)
    • EOS flyer
  • EVENTS
    • Customer webinar - Jan Remsik, MSKCC (July 19)
    • Corporate events through EOY

​

July 6, 2023 August 30, 2023

Confidential – For Internal Use Only

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EOS Messaging Strategy

12

EOS in Greek mythology is the personification of the dawn, the sister of the sun. It’s a no-brainer to leverage this messaging to make it powerful, inspiring, and memorable. We can separate ourselves from the pack with great creative centered on advantages and benefits to researchers, reinforced by EOS features, and supported by Levitation Technology science.

CREATIVE

MESSAGING

LeviCell® EOS

�Experience MORE to the Power of 4

�1. Expand your sample prep throughput—run 4 samples simultaneously

2. Extend your sample processing capacity— connect 4 instruments

3. Examine your sample quality in real-time

4. Empower your research— sample viability up to 99%, 5x lower dead cells and debris, and the ability to start with as few as 8000 cells

�

Confidential – For Internal Use Only

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The Need For LeviCell EOS

With regard to LeviCell 1.0 → “I’d really like to have...”

  1. Higher throughput sample prep
  2. Temperature control → Nuclei work

High throughput screening

Analytical end assay

>96 samples

Not the same as

High throughput sample prep

Processing step

<16 samples

8

2

16

16

96

Confidential – For Internal Use Only

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Already Talking To LeviCell EOS Target Customers

Filling the gap where other techniques fall short

Answering call for difficult samples

Single Cell Genomics

Key

Target Areas

Cryopreservation Rescue

Flow Cytometry

Cancer Models

Confidential – For Internal Use Only

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Instrument Portfolio

LeviCell 1.0

LeviCell EOS

  • Introductory affordable system
  • Single sample processing
  • Primary instrument to demo Levitation Technology to new customers
  • Fixed point visualization of levitating sample
  • Compact footprint, fits in biosafety hood
  • Fractionation analysis
  • More advanced system for expansion
  • Parallel sample processing by 4x
  • Scalability up to 4 systems together
  • Temperature control option
  • Visualize entire separation channel
  • Flexibility to address future applications
  • Enhanced in run characterization capabilities

Confidential – For Internal Use Only

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Comparison Table

​

Levicell 1.0

LeviCell EOS

# of samples/run

1

4

Temperature Control

No

Yes*

Visualization

Fixed Position

Full Separation Channel

Cartridge Prescan Required

No

Yes

Fractionation Analysis

From Image Collection

From Lane 1 Image Collection

Cartridge

S2.3, S2.3 Sterile

EOS-4, EOS-4 Sterile*

Sterile Inlet Covers

No

Yes

Magnetic Core

Fixed

User exchangeable

Clamping

Manual

Automatic

Illumination

Brightfield (transmitted illumination)

Ex470 nm, Em524/29 nm (eg Calcein)

Ex560 nm, Em 628/33 nm (e.g. PI)

Brightfield (transmitted illumination)

Ex470 nm, Em524/43 nm (eg Calcein)

Ex560 nm, Em 628/65 nm (e.g. PI)

Instrument Control Software

Experiment Manager

EOS Manager

Standalone Analysis Software

Experiment Analyzer

LeviMetrics*

Biosafety Cabinet Compatibility

Standard

Custom

Footprint (W x D x H)

47 cm x 34 m x 24 cm

19” x 13” x 19”

44 cm x 63 cm x 46 cm

17.3” W x 24.8” D x 18.1” H

Confidential – For Internal Use Only

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Who Else Is in Our Space?

Cell Sorting

Magnetic Bead

Other

Confidential – For Internal Use Only

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Key Differentiating Factors

​

​

​

​

​

​

​

​

Technology:

Label-free enrichment

Non-Annexin enrichment

No change to cells

​

✅

✅

✅

​

✅

✅

✅

​

🆇

🆇

​

​

🆇

​

🆇

​

✅

✅

≈

​

✅

✅

≈

​

🆇

​

​

Parallel Processing

🆇

✅

​

🆇

✅

🆇

🆇

Visualize cells & sample

✅

✅

🆇

🆇

🆇

🆇

🆇

Single use consumable

✅

✅

✅

🆇

✅

✅

✅

Target enrichment

✅

✅

✅

✅

🆇

✅

✅

Debris removal (auto)

✅

✅

🆇

​

​

​

≈

Ease of Use

✅

✅

​

🆇

✅

​

​

Flexibility to expand

🆇

✅

✅

​

🆇

🆇

🆇

Nuclei compatible

✅

✅

✅

✅

✅

✅

✅

≈

≈

≈

≈

≈

≈

≈

≈

≈

≈

≈

✅ Available

🆇 Not available

Possible/relative

≈

Cell Washing

Cell Dispensing

≈

≈

≈

≈

Confidential – For Internal Use Only

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LeviCell EOS Service Plans Pricing

PN

Name

Description

INTL List Price

Transfer Price

1110010

LeviCell EOS Extended Service Plan, 1 Year Coverage

1-year extended service plan, includes one onsite PM

$17,825

$12,478

1110011

LeviCell EOS Extended Service Plan, 2 Years Coverage

2-year extended service plan, includes one onsite PM per year

$32,200

$22,540

1110012

LeviCell EOS Extended Service Plan, 3 Years Coverage

3-year extended service plan, includes one onsite PM per year

$46,000

$32,200

1110013

LeviCell EOS Mid-Year Preventive

Maintenance (PM)

Additional one time PM outside of standard support or service

NOTE: Must be on a warranty or service plan

$6,325

$4,428

1110014

LeviCell EOS Bridge Service Plan, 1 Month Coverage

1 month additional service plan (bridge timeframe)

NOTE: Must only be sold with 1 year service plan

$1,484

$1,038

1110015

LeviCell EOS Resume Service Plan with Assessment Plus Repair, 1 Year Coverage

Includes one onsite PM, any repairs needed prior to resuming 1 year service coverage

$28,750

$20,125

1110016

LeviCell EOS One-time Preventative Maintenance

(not on service plan)

One time onsite PM check out

(For those not on service plan only)

$11,500

$8,050

1110017

LeviCell EOS Repair Only (not on service plan)

One time onsite service repair (For those not on service plan only, minimum charge applied+material)

Min- $5,500 + Material

​

1110018

LeviCell EOS Core Extended Service Plan, 1 Year Coverage

1-year extended service plan, includes one onsite PM

$2,300

$1,610

1110019

LeviCell EOS Core Bridge Service Plan, 1 Month Coverage

1 month additional service plan (bridge timeframe)

NOTE: Must only be sold with 1 year service plan

$190

$133

1110020

LeviCell EOS Base Module Bridge Service Plan, 1 Month Coverage

1 month additional service plan (bridge timeframe)

NOTE: Must only be sold with 1 year service plan

$11,500

$8,050

Confidential – For Internal Use Only

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Support Plan

Confidential – For Internal Use Only

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Levitation Technology is the Same on Both Systems

With LeviCell EOS you can:

  • Minimize sample processing time
    • Obtain 4x more cells or process 4 different samples at a time
  • Switch To/From LeviCell 1.0 & LeviCell EOS
    • Consistent performance across systems provides same confidence in data quality
  • Make decisions best samples for downstream analysis
    • Maximize resources spent to execute time-consuming and expensive experiments

​

​

Total of 89 runs tested

Confidential – For Internal Use Only

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Levitation Technology is the Same on Both Systems

Demo validation of how our technology works

  • Are the customers new to our technology?
  • Do they have any experience with our products (via a core or previous demo interaction with another lab?)
  • What are their needs for sample prep improvement?
  • What is the measurable success criteria they want to see?

Why you can demo with the LeviCell 1.0

  • Already own a LeviCell 1.0
  • Smaller system, easy to transport
  • Same transferable technology
  • Software is the main difference between systems
  • No need to showcase throughput

Confidential – For Internal Use Only

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EOS Manager GUI Demo Tool

EOS Manager

GUI Demo and Guide

Why would you use this GUI Demo software?

  • Show the new EOS Manager software
  • Describe the intuitive, step by step setup
  • Explain full separation channel visualization and how it enables sample characterization
  • Show different options for viewing sample
  • Reinforce the ease of use of Levitation Technology

​

​

Confidential – For Internal Use Only

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Method to “Demo” LeviCell EOS

Current

  • Knows all about our technology
  • New to portfolio additions
  • Demo LeviSelect
  • Higher throughput

Demo LeviSelect using customer’s LC1

  • Bring to reference site or core facility
  • Showcase on virtual demo tool

Current

  • Knows all about us
  • Higher throughput

​

  • Bring to reference site or core facility
  • Showcase on virtual demo tool

Customer

Customer Knowledge

Customer Interests

Step 1

If close on step 1 & qualified needs for higher throughput

New

  • New to Levitation Technology
  • New to portfolio
  • General sample processing needs

Demo Levitation Technology using LC1

​

​

​

​

  • Bring to reference site or core facility
  • Showcase on virtual demo tool

​

​

​

​

New

  • Aware of technology/portfolio but no firm understanding
  • Never tried or seen our system in lab setting
  • General sample processing needs
  • Higher throughput

New

  • Aware of technology/portfolio, has investigated our technology
  • Peripheral experience through demo or colleagues but never used their own samples
  • Has solid req for sample processing needs
  • Higher throughput

Confidential – For Internal Use Only

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Closer Look at LeviCell EOS

Confidential – For Internal Use Only

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LeviCell EOS Future Timeline Update

Q1 2023

Q2 2023

Q3 2023

Q4 2023

Q1 2024

Commercial Release

Ship June 30th

Trade-In

offered

Trade-In offer up to $35,000

Exp June 15, 2023

LeviCellEOS

LeviMetrics

Commercial Release

v1.0.3

EOS Manager

Q2 2024

Viability Metric

Counting Metric

(License restricted)

Updates

Fixed Point Fractionation

TEC Core

Target EOY

Sterile Covers

Independent split lines

Q4

Confidential – For Internal Use Only

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Future Enhancements Planned for LeviCell EOS/EOS-4 Cartridge

27

Post-Commercial Feature Development

​

Future release updates (~6 months)

  • Temperature control (cold) core
  • Ability to run unused lanes of a cartridge
    • New cartridges to support this feature

​

Future product development

  • Application-specific cores
  • Additional LeviMetrics analysis methods

Confidential – For Internal Use Only

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Cartridge Enables Parallel Processing & Maximizes Volume Recovery

28

  • Single use cartridge that allows optical system to scan the full channel for all 4 samples
  • Easily scale from 1-4 samples or 4x1 sample for higher cell number samples
  • Same reliable performance seen with LeviCell 1.0

Outlet Wells

Separation Channels

Inlet Wells

Cartridge Grip

Confidential – For Internal Use Only

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What’s Different About The EOS-4 Cartridge

  • Modified funnel inlet well for less sample loss
  • Sunken outlet well uses gravity to our advantage
  • Removal of serpentine path to outlet well

LeviCell EOS

EOS-4 cartridge

Side view

LeviCell 1.0

S2.3 Cartridge

Cross section of the outlet well

3 point collection

Cross section of the inlet well

Cross section of the outlet well

Confidential – For Internal Use Only

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EOS-4 Cartridges

Confidential – For Internal Use Only

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Reagents and Starter Kit

Levitation Agent, 40 Rxn

​

LeviCell EOS Installation and Calibration Kit

​

EOS-4 Cartridges 10 pk

​

EOS-4 Cartridges, Sterile 10 pk

EOS Starter Kit (Q3)

​

  • 5 pk of EOS-4 cartridges
  • LeviCell EOS Installation and Calibration Kit
  • Levitation Agent, 10 rxn (2x)

Confidential – For Internal Use Only

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How Images Are Captured On LeviCell 1.0

Fixed

camera position

1

2

3

4

5

6

7

2

3

4

5

6

7

3

4

5

6

7

4

5

6

7

5

6

7

6

7

7

1

2

3

4

5

6

7

1

Concatenated images during collection used to create movie & montage

As sample flows by during collection, image is captured

FOV

7 image montage

Confidential – For Internal Use Only

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Scanning the Cartridge on the LeviCell EOS

9 scan positions along the lane

1

2

3

4

5

6

7

8

9

Separation channel

cross section

Brightfield

Camera

Periscope

Mirrors

Confidential – For Internal Use Only

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Scanning the Cartridge

Scan Lane 1

Scan Lane 2

Scan Lane 3

Scan Lane 4

9 scan positions along the lane

~90 sec for a complete scan

~18 completed scans for a medium cell run (20 mins)

Confidential – For Internal Use Only

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Future Product Development – User Exchangeable Cores

What does this mean?

  • Temperature control (cold)
  • Different magnet configuration
  • Different cartridge configuration

​

What does this enable?

  • Application-specific magnet configurations
  • Change number of samples run
  • Change scale of sample run
  • Ability to divert output sample back to levitation
  • Introduction of buffers/sample/additives

Confidential – For Internal Use Only

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LeviCell EOS: Visualizing New Frontiers in Sample Preparation

36

  • Extract meaningful data sample characterization upstream of any additional downstream analysis

​

  • Provide images of the full separation channel for the entire run
  • Only system where samples can be visualized during the sample preparation process

LeviCell EOS

EOS Manager Software

Confidential – For Internal Use Only

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EOS Module Communication to EOS Manager

EOS Module has its own computer inside

EOS Manager reflects what’s occurring on the EOS Module

​

In the event of a software crash or Control PC crashes reboot, the EOS Module will continue to finish programmed step till next user intervention

Confidential – For Internal Use Only

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Instrument LCD Display

Confidential – For Internal Use Only

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Run Status Screen - Levitating Samples

Confidential – For Internal Use Only

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Run Status Screen - Levitation Ready

Confidential – For Internal Use Only

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Lane Detail

Leading Meniscus

Trailing Meniscus

Center Line

(stabilizing pin)

LC1 Image

LC1’s camera position is after the meniscus, no splitter is shown in the view

​

LeviCell EOS, splitter is seen as well as leading and trailing meniscus

  • The whole sample volume can be seen

Confidential – For Internal Use Only

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2 Step Zoom Feature

Zoom Level 1:

True aspect ratio of the channels

Zoom Level 2:

Full channel height on the screen

Confidential – For Internal Use Only

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Fluorescence Views

Confidential – For Internal Use Only

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Collection Step

Only Lane 1 will be viewed during collection

  • Recording of collection will occur similar to LC1
  • No fractionation data will be available at launch

All images captured will be viewable via Levimetrics

Confidential – For Internal Use Only

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Run History & Run Files

  • View Run Summary
  • Download Run Summary
  • View file location
  • Delete Run

The Run.Exp is a special file format that is used with LeviMetrics software

Reports Folder

Confidential – For Internal Use Only

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Future of LeviMetrics is to Support All Instrumentation

LeviMetrics

Standalone

Analysis Software

Experiment Analyzer

Standalone

Analysis Software

Future merge

Confidential – For Internal Use Only

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LeviMetrics Viewer - Levitation

47

Viewing options

View each scan

Open multiple run files

Confidential – For Internal Use Only

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LeviMetrics Viewer - Collection

48

Viewing options

View each scan

Confidential – For Internal Use Only

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Possible Future Capabilities Using Sample Characterization

  • Morphological + Density analysis

​

​

​

  • Expression analysis

​

​

​

​

  • Time course analysis

​

​

​

  • Levitation analysis

​

Protocol Optimization

Where and how much?

Cell-activation study

  • Enrichment metrics

​

​

  • Purity metrics

​

​

​

  • Quality

​

​

​

  • Count

​

​

​

​

​

​

​

​

​

What percentage of cells are live cells?

42,000

238,000

15%

85%

Enrichment + cell count in one step.

Low quality

High quality

High Purity

Sample Composition.

Quality Control.

What are the states of the organoids

Epithelial Viability stain

LA Modification

Day 1

Day 10

Differentiated

Un-differentiated

Harness more power from our images with sample characterization methods

Confidential – For Internal Use Only

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Future Feature Additions & Software Packages

LeviMetrics

  • Video- export file
  • Expt Viewer
  • Fractionation
  • Levitation Analysis
  • Quality Metric
  • Sample Composition

Free

LeviMetrics Elite*

  • Video- export file
  • Expt Viewer
  • Fractionation
  • Levitation Analysis
  • Quality Metric
  • Sample Composition

​

  • Viability Metric
  • Counting
  • Expression Analysis
  • Morphological Metric
  • Density Analysis

$ Upgrade

LeviMetrics Pro*

  • Video- export file
  • Expt Viewer
  • Fractionation
  • Levitation Analysis
  • Quality Metric
  • Sample Composition

​

  • Viability Metric
  • Counting
  • Expression Analysis
  • Morphological Metric
  • Density Analysis

​

  • LevitasBio Cloud Access
  • High Throughput Analysis
  • Discovery Analysis

$ Upgrade

*Not available yet

Confidential – For Internal Use Only

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Product and Pricing Changes

Confidential - Internal Use Only

Confidential – For Internal Use Only

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Product and Pricing Changes

  • New early access LeviSelect Immune Cell Kits
    • Human NK Enrichment
    • Human Naïve and Memory T Cell Enrichment
    • Mouse EPCAM Depletion

Confidential – For Internal Use Only

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Product and Pricing Changes

  • Replacement product for Demos/Starting Kit

​

Discontinued Product

Replaced with

1003101 LeviCell Demo Kit

PN 1003103 LeviCell 1.0 Starter Kit

1003102 LeviCell Demo Kit, sterile

PN 1003104 LeviCell 1.0 Starter Kit, sterile

1002001 LeviCell Cartridges, S2.1 (10-pack)

1002010 LeviCell Cartridges, S2.3 (10-pack)

1002002 LeviCell Cartridges, S2.1-IR (10-pack)

1002012 LeviCell Cartridges, S2.3-IR (10-pack)

​

​

DO NOT USE Product Number

Replaced with

1000020 LeviCell EOS TEC System w/ 1 year warranty

1000021 LeviCell EOS System w/ 1 year warranty

+

1000025 LeviCell EOS-4 TEC Core w/ 1 year warranty

Confidential – For Internal Use Only

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Complete List of EOS PNs & New Pricing

​

​

​

INtL List

Transfer Price

1000021

LeviCell EOS System

1 ea

$131,250

$91,875

1000025

EOS-4 TEC Core

1 ea

$26,250

$18,375

1020001

LeviMetrics Analysis Software Elite Upgrade

1 ea

$11,500

$8,050

1002101

LeviCell EOS-4 Cartridge (10 pack)

1 ea

$2,070

$1,449

1003002

Levitation Agent (40 rxn)

1 ea

$460

$322

1003004

LeviCell EOS Installation and Calibration Kit

1 ea

$1,150

$805

​

​

​

​

​

1000020

LeviCell EOS TEC System

1 ea

$152,250

$106,575

1000024

LeviCell EOS Base Module

1 ea

$120,750

$84,525

1000026

LeviCell EOS-4 Core

1 ea

$10,500

$7,350

​

​

​

​

​

1110021

LeviCell EOS Installation & Training

1 ea

$5750

$4025

​

​

​

​

​

Confidential – For Internal Use Only