CellularCT Project
Jasmine Bueno, Daniel DeMaster, Chris Gorzynski, Nancy Huang
Fig. A CAD rendering of the modular cartridge system, featuring interchangeable syringe-driven input modules and a central imaging chamber. The design supports customizable workflows and streamlined fluid delivery for diagnostic assays
Fig D. Design Matrix showing the customer requirements, engineering requirements, and their importance rankings as compared to an industry standard benchmark.
Fig F. Volume loss from the Leak Test demonstrated there is some volume loss in both the 3D printed cartridge without attached tubing and in the cartridge with attached tubing. There is no statistically significant difference between the two cartridges however, the sample with attached tubing has a higher average volume loss.
Fig G. Full Assembly Drawing showing the Solidworks drawing of all pieces. Including the workflow base, aliquot pieces, reservoir, plungers, and cuvette.
Background/Objectives
The Cellular CT Device is an instrument indicated for use by trained technicians in mid-low income countries for disease diagnosis. The instrument integrates light microscopy, fluorescence cytometry, and machine learning to rapidly analyze key biomarkers in blood samples without requiring trained professionals to interpret data. The Cellular CT Device is intended for rapid and accurate diagnosis of diseases through automated analysis.
The objective of this project is to design and manufacture an automated, disposable, modular cartridge that works in tandem with the Cellular CT device to analyze blood samples.
�Design Specifications
Design Process
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BIOMEDICAL ENGINEERING
Prototype Testing
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Conclusion & Future Directions
The development of the modular cartridge for the CellularCT system successfully demonstrated a low-cost, flexible solution for sample preparation and imaging in resource-limited settings. The design met key requirements for modularity, portability, and manufacturability, with successful initial testing confirming its functionality. Moving forward, future work will focus on integrating automated fluid control, expanding the cartridge's compatibility with a broader range of diagnostic assays, and conducting clinical validation with biological samples. These efforts aim to enhance the device’s impact in global health diagnostics by improving accessibility and diagnostic accuracy.
Customer Needs | Engineering Metrics | ||
Low Cost | Nondestructive Testing | Manufacturing process compatibility | Compact design |
Easy to use system design | Easily replaceable parts | Power consumptions | Particle Size detection |
No fluidics | Dark housing/enclosed design | Rechargeable battery | Design flexibility |
Easy storage, transportation | Detects different diseases | Dry sample processing | � |
Detector Sensitivity | High throughput | Dual mode detection | � |
Fig E. Leak tests that were done on some of the cartridge components.
Prototype Manufacturing
Fig B. Initial Prototype iterations with one iteration incorporating samples rotating on a wheel to automate the sample processing and the second iteration featuring a central revoir and samples surrounding it
Fig C. Assembled final prototype with a coded arduino microcontroller, breadboard, and servo motors to automate the white blood cell workflow process
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