How to improve the importance of metadata from the clinic to the lab
Sara Samari and Michela Noseda
CLINICAL DATA
EXPERIMENTAL DATA
HISTOLOGY DATA
Donor | D1 |
Sex | Female |
Age | 50-55 |
Ethnic Origin | Caucasian |
Primary diagnosis | CVA (stroke) |
Cause of death (DBD or DCD) | DBD |
NRP (time) | N/A |
Height (cm) | 155-160 |
Weight (Kg) | 50-55 |
Girth (cm) | 80-85 |
BMI | 20-25 |
Hypertension | Y |
Diabetes | N |
Cancer | N |
Pulmonary disease | N |
Liver disease | N |
Echocardiography (LVEF %) | >60% |
Donor | D1 |
Cells | 4500 |
Genes | 1570 |
Reads | 44000 |
Source | Cells/Nuclei |
Target Number of cells/nuclei | 5000 |
Region | LV, RA, etc |
Staining | Target |
H&E | Nucleus/cytoplasm |
Elastic van Gieson (EVG) | Connective tissue (collagen and elastic fibres)/ fibrosis |
Congo Red | Amyloid |
Donor | Dx | Dy |
Sex | Female | Male |
Age | 65-70 | 50-55 |
Ethnic Origin | Caucasian | Caucasian |
Primary diagnosis | Intracranial haemorrhage | Ruptured cerebral aneurysm |
Cause of death | DCD | DBD |
NRP (time) | n/a | N/A |
Donor type | DCD | - |
Height (cm) | 155-160 | 175-180 |
Weight (Kg) | 80-85 | 70-75 |
Girth (cm) | 100-105 | N/A |
BMI | 30-35 | 20-25 |
Infections |
|
|
| + | - |
| + | + |
| - | - |
| - | - |
| - | - |
| - | - |
Infection at admission | Yes |
|
| aspiration infection | N/A |
| No | No growth |
| No | No growth |
| No | No growth |
| No | No growth |
| No | candida albicans isolated. No bacteria seen. |
| Yes | N |
| No | 1+yeast. 1+ mixed oropharangeal flora |
| No | 4+ polymorphs. 1+ epithelial cells, 1+ RBCs. No bacteria seen. |
| n/a | N |
|
| N |
Donor | Dx | Dy |
Hypertension | No | N |
Cancer or malignancy | No | N |
Pulmonary disease | No | N |
Liver disease | No | N |
Diabetes | No | N |
| n/a | N/A |
| Yes | N |
Alcohol use (duration) | Drinks rarely | Y (35yrs) |
| <1u/day | 6 beers every couple days |
History of drug use? | No | Y |
|
| occasionally |
Current or past smoker | Non-smoker | Y |
| n/a | 40yrs |
| n/a | 1ppd |
| n/a | N/A |
Allergies? |
| N |
| Diclofenac and citalopram | N/A |
Heart | no tests conducted |
|
|
| sinus rhythm, unspecific ST-T wave abnormalities, prolonged QT interval |
|
| LVEF >60% |
| 10-15 | <0.1 |
|
| N/A |
|
| N/A |
|
| N/A |
Coagulation |
|
|
| 25-30 | 30-40-35 |
| 10-15 | 1.0-1.5 |
|
| N/A |
|
| |
| 10-15 | 7.5-2.5-10.5 |
| 1-5 | 1.0-1.65 |
| 20-25 | 35-30 |
| 50-55 | N/A |
| 150-155 | 140-155-145 |
| 1-5 | 3.5-4 |
|
| 30-18 |
| 5-10 | 5-16-15 |
| 115-120 | 105-210-155 |
| 50-55 | N/A |
| 10-15 | N/A |
| n/a | N/A |
| 10-15 | 20-35-25 |
Donor | Dx | Dy |
Complete blood count |
|
|
|
| 9.0-7.0 |
| 1-5 | 0.8-2 |
|
| 0.1 |
|
| 0 |
|
| 1-1 |
|
| 4.5-4.0 |
|
| 90-95-95 |
|
| N/A |
|
| 335-325 |
|
| 13-15 |
| 100-105 | 145-120 |
| 0.30-0.35 | 0.4-0.4 |
| 65-70 | 296-40 |
Inflammation parameters |
|
|
|
| N/A |
| 50-55 | N/A |
Histological METADATA
Histological METADATA – RIGHT ATRIA: a complementary staining approach
Masson's trichrome, 20x
Congo Red, 20x
Picrosirius Red, Polar, 20x