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How to improve the importance of metadata from the clinic to the lab

Sara Samari and Michela Noseda

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CLINICAL DATA

EXPERIMENTAL DATA

HISTOLOGY DATA

Donor

D1

Sex

Female

Age

50-55

Ethnic Origin

Caucasian

Primary diagnosis

CVA (stroke)

Cause of death (DBD or DCD)

DBD

NRP (time)

N/A

Height (cm)

155-160

Weight (Kg)

50-55

Girth (cm)

80-85

BMI

20-25

Hypertension

Y

Diabetes

N

Cancer

N

Pulmonary disease

N

Liver disease

N

Echocardiography (LVEF %)

>60%

Donor

D1

Cells

4500

Genes

1570

Reads

44000

Source

Cells/Nuclei

Target Number of cells/nuclei

5000

Region

LV, RA, etc

Staining

Target

H&E

Nucleus/cytoplasm

Elastic van Gieson (EVG)

Connective tissue (collagen and elastic fibres)/ fibrosis

Congo Red

Amyloid

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Donor

Dx

Dy

Sex

Female

Male

Age

65-70

50-55

Ethnic Origin

Caucasian

Caucasian

Primary diagnosis

Intracranial haemorrhage

Ruptured cerebral aneurysm

Cause of death

DCD

DBD

NRP (time)

n/a

N/A

Donor type

DCD

-

Height (cm)

155-160

175-180

Weight (Kg)

80-85

70-75

Girth (cm)

100-105

N/A

BMI

30-35

20-25

Infections

 

 

        • CMV

+

-

        • EBV

+

+

        • Toxo

-

-

        • HIV

-

-

        • HCV

-

-

        • HBV

-

-

Infection at admission

Yes

 

  • Diagnosis

aspiration infection

N/A

  • Urine

No

No growth

  • Blood

No

No growth

  • Blood cultures result

No

No growth

  • CNS/CSF

No

No growth

  • Chest

No

candida albicans isolated. No bacteria seen.

  • Treated for chest or aspiration

Yes

N

  • Purulent sputum

No

1+yeast. 1+ mixed oropharangeal flora

  • Donor sputum microscopy

No

4+ polymorphs. 1+ epithelial cells, 1+ RBCs. No bacteria seen.

  • Other infections

n/a

N

  • Antibiotics (in the last two weeks prior to death )

 

N

Donor

Dx

Dy

Hypertension

No

N

Cancer or malignancy

No

N

Pulmonary disease

No

N

Liver disease

No

N

Diabetes

No

N

  • Insulin, medicaton or diet controlled

n/a

N/A

  • Family history of diabetes

Yes

N

Alcohol use (duration)

Drinks rarely

Y (35yrs)

  • Units of alcohol

<1u/day

6 beers every couple days

History of drug use?

No

Y

  • Drug details

 

occasionally

Current or past smoker

Non-smoker

Y

  • Smoking (duration)

n/a

40yrs

  • Number of Cigarettes per day

n/a

1ppd

  • Date stopped smoking (if applicable)

n/a

N/A

Allergies?

 

N

  • Allergy details

Diclofenac and citalopram

N/A

Heart

no tests conducted

 

  • ECG

 

sinus rhythm, unspecific ST-T wave abnormalities, prolonged QT interval

  • Eco (EF etc)

 

LVEF >60%

  • Troponin I ng/L

10-15

<0.1

  • CK-MB?

 

N/A

  • Myoglobin

 

N/A

  • BNP or NT-proBNP

 

N/A

Coagulation

 

 

  • aPTT (s)

25-30

30-40-35

  • PT (prothrombin)

10-15

1.0-1.5

  • TT

 

N/A

 

 

  • Glucose

10-15

7.5-2.5-10.5

  • Calcium

1-5

1.0-1.65

  • Albumin

20-25

35-30

  • Total proteins

50-55

N/A

  • Sodium (mmol/L)

150-155

140-155-145

  • Potassium (mmol/L)

1-5

3.5-4

  • CO2

 

30-18

  • BUN (blood urea nitrogen) (mmol/L)

5-10

5-16-15

  • Creatinine (umol/L)

115-120

105-210-155

  • ALP

50-55

N/A

  • ALT

10-15

N/A

  • AST

n/a

N/A

  • Bilirubin

10-15

20-35-25

Donor

Dx

Dy

Complete blood count

 

 

  • Neutrophils

 

9.0-7.0

  • Lymphocytes [ white cell count 10^9/L]

1-5

0.8-2

  • Basophils

 

0.1

  • Eosinophils

 

0

  • Monocytes

 

1-1

  • RBC count

 

4.5-4.0

  • MCV

 

90-95-95

  • MCH

 

N/A

  • MCHC

 

335-325

  • RDW

 

13-15

  • Hb

100-105

145-120

  • Hct

0.30-0.35

0.4-0.4

  • Platelet count

65-70

296-40

Inflammation parameters

 

 

  • ESR

 

N/A

  • CRP

50-55

N/A

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Histological METADATA

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Histological METADATA – RIGHT ATRIA: a complementary staining approach

Masson's trichrome, 20x

Congo Red, 20x

Picrosirius Red, Polar, 20x