The Greenbrier River - Protecting It for the Future��
An overview of water quality monitoring
August 2023-October 2025
Matthew Williams PhD
Water Quality
Public Domain (CC0 1.0)
Water and Health
Public Domain Mark 1.0
Water and Health
https://www.flickr.com/photos/sniegowski/51186578680
(CC BY-NC 2.0)
Public Domain
PHOTOGRAPHED BY JESSE THORNTON
https://wvtourism.com/9-must-visit-spots-in-the-greenbrier-valley/
https://greenbrierwv.com/editorials/roadtrip-along-the-greenbrier-river
https://wvexplorer.com/attractions/rivers-streams/greenbrier-river/
Water Quality
Biological
Physical/Chemical
Parameters for water quality are determined by the intended use.
Microbiology of Water
Bacteria
Viruses
Protozoa
Fungi
Algae
Helminths
What is a healthy waterway?
Traditional
Newer studies
Microbiota of waterways
Lab culture vs. genetic sequencing
Lee, et al 2016
Water related diseases
Water related diseases
Water treatment
Coliforms- a group of bacteria commonly inhabiting the GI tracts of mammals.
Clean water access led to the largest decline in human infectious disease in recent human history
Figure 1. Crude death rate per 100,000 for infectious diseases - United States, 1900-1996 CDC- https://www.cdc.gov/healthywater/drinking/history.html
Water access
WVDEP 2022 Report
Images from GRWA QAPP
All 162 miles of the Greenbrier River have been listed as “impaired” for coliforms since 2002. Still listed in 2022 report.
What do we do about this?
Data gathered
Monthly monitoring
Image source: Hannah Instruments
Image source: Micrology Laboratories
Site ID | Full Name | Testing Location | Latitude | Longitude |
CON-1 | Confluence 1 | Confluence of East and West Greenbrier | 38.5437604 | -79.8320161 |
C-1 | Cass 1 | Cass Boat Launch | 38.39692886 | -79.91354901 |
M-1 | Marlinton-1 | Marlinton at Stillwell Park | 38.22438781 | -80.09543826 |
WAT-1 | Watoga 1 | Watoga State Park | 38.1181736 | -80.1746816 |
BUR-1 | Burnside 1 | Burnside | 38.1080971 | -80.208036 |
R-1 | Renick-1 | Renick Boat Launch | 37.98985137 | -80.35662681 |
SC-1 | Spring Creek-1 | Spring Creek Con\fluence | 37.9532070 | -80.3462650 |
AN-1 | Anthony-1 | Anthony Boat Launch | 37.89589096 | -80.32506539 |
CAL-1 | Caldwell-1 | Caldwell Boat Launch | 37.78192896 | -80.39831387 |
MON-1 | Montwell-1 | Montwell - Lewisburg Sink | 37.8024952 | -80.4420681 |
RON-1 | Ronceverte-1 | Ronceverte Boat Launch | 37.74480623 | -81.46642891 |
FS-1 | Fort Springs-1 | Fort Springs Boat Launch | 37.75266063 | -80.54656262 |
D-1 | Davis Spring 1 | Davis Spring | 37.755.3922 | -80.5451873 |
ALD-1 | Alderson 1 | Alderson Boat Launch | 37.72649203 | -80.64999208 |
TAL-1 | Talcott 1 | Talcott Boat Launch | 37.64934687 | -81.75134638 |
ANG-1 | Angler Lane 1 | Anglers Lane Boat Launch | 37.6149906 | -80.77559493 |
W-1 | Willowwood 1 | Willow Wood Boat Launch | 37.64376424 | -80.80654929 |
HIN-1 | Hinton-1 | Willowwood Road Bridge | 37.652254 | -80.885065 |
Detailed Locations of Sampling Sites
Credit: Tom Saladayga PhD
Temperature pollution can impact some waterways. Doesn’t appear we have any issues with this. Trend is warmer as moves downstream which is usual.
pH is a measure of acid or alkalinity of the water. Impacts such as mining or runoff can impact pH. Acid mine drainage is an issue in many watersheds where mining is present. Limestone in karst formations can make water more alkaline. We are in the ideal range for aquatic life.
TDS includes various salts, minerals, organics, and other solids dissolved in water. While TDS itself doesn't indicate harmful contaminants, very high levels can affect water clarity and taste, and different aquatic organisms have a preferred range they must stay within for optimum health.
Conductivity is a measure that assesses the ability of water to conduct electricity and this capacity can be increased by charged chemicals such as salts being dissolved in the water. Potential charged chemicals include calcium, chloride, nitrate, potassium, and sulphate.
Dissolved oxygen (DO) is essential for the survival of aquatic organisms, as it supports the respiration of fish, plants, and other aquatic life. Adequate DO levels help maintain healthy ecosystems, while low levels (hypoxia) can lead to stress, disease, or death for aquatic species and contribute to problems like fish kills and harmful algal blooms.
Oxidation-Reduction Potential (ORP) is a measure of capacity of water to remove contaminants, as such, lower numbers indicate a low capacity for contaminant removal. Possible causes for low ORP include: industrial wastewater, organic contamination, high concentration of phosphates and algae growth, or sewage input.
E. coli testing in recreational waters detects potential fecal contamination, indicating potential health risks from harmful pathogens. It helps ensure water safety and informs decisions or advisories to protect public health.
Geomean- bacteria are measured on a log scale. Geomean also calculates in the months there were not exceedances.
Recreational water quality guidelines suggest numbers should not exceed 200cfu/100mL
U.S. Department of Agriculture
National Institute of Allergy and Infectious Diseases, National Institutes of Health
E. coli testing in recreational waters detects potential fecal contamination, indicating potential health risks from harmful pathogens. It helps ensure water safety and informs decisions or advisories to protect public health.
Geomean- bacteria are measured on a log scale. Geomean also calculates in the months there were not exceedances.
Recreational water quality guidelines suggest numbers should not exceed 200cfu/100mL
Credit: Tom Saladayga PhD
E. coli testing in recreational waters detects potential fecal contamination, indicating potential health risks from harmful pathogens. It helps ensure water safety and informs decisions or advisories to protect public health.
Geomean- bacteria are measured on a log scale. Geomean also calculates in the months there were not exceedances.
Recreational water quality guidelines suggest numbers should not exceed 200cfu/100mL
E. coli testing in recreational waters detects potential fecal contamination, indicating potential health risks from harmful pathogens. It helps ensure water safety and informs decisions or advisories to protect public health.
Percent Exceedance shows the number of sampling events (months), where there was an exceedance of 200 cfu/100mL at each site
So even though the mean is low, there are still months where some sites give a bit of concern.
E. coli testing in recreational waters detects potential fecal contamination, indicating potential health risks from harmful pathogens. It helps ensure water safety and informs decisions or advisories to protect public health.
Recreational water quality guidelines suggest numbers should not exceed 200cfu/100mL
Average of Exceedances shows the average values when there are exceedances
Target those that often exceed standard, and have high average values
The less than ideal numbers…
Targeted Sampling-Source Identification- Montwell
Source: Emma Hughes OMS-II WVSOM
Targeted Sampling-Source Identification- Montwell
Targeted Sampling-Source Identification- Marlinton
Very preliminary!!! Not enough data points
The most ideal numbers…..
36 volunteers on the project
Several WVSOM Students currently using this as a public health research project
Numerous community members regularly assist with monitoring efforts
800+ hours!
=$27,832 value
Having a monitoring team allows tangential monitoring- PFAS, etc
Volunteer valuation - https://independentsector.org/research/value-of-volunteer-time
Summary