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Welcome Back to Biology with Ms. Lassar!

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1. 6th period: Take out your cartoon
2. Show your cartoon to your table and explain what you drew.
3. Tablemates: Ask questions until you understand the cartoon your groupmate created

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Agenda 11/28/16

Objective: Students will review the DNA barcoding procedure

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• DNA Barcoding Introduction
• Finish Abnormal Cell Cycle

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Homework:

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1. None :)

We need to take a pitstop and prepare for a future project

What is the DNA barcoding project?

Purpose of this project

• We will be using DNA barcoding to identify plants on B-CC school grounds.
• You all will essentially be taking an inventory of the schools grounds to determine if there are invasive species present.
• You will be taking a sample of a suspected invasive species, extracting the DNA, and sending it to a lab to barcode the species.
• This data will then be compared to all other Montgomery County Public High Schools for analysis of trends.

Why is this important?

• Identifying organisms has grown in importance as humans monitor the biological effects of global climate change and attempt to preserve species diversity in the face of accelerating habitat destruction.

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• But invasive species can harm the preservation of species diversity and can be detrimental to the success of a habitat

Okay…but what is an invasive species?

• We will be learning more about invasive species in the second semester, but here is some basic info:
• Invasive species are organisms that are accidentally brought into a community where they then flourish and take away resources that the native inhabitant organisms use
• Invasive species slowly eliminate the native species, contributing to and accelerating the destruction of habitats
• Invasive species can be parasitic, predatory, or indirectly harmful as plants or animals

DNA barcoding

• Before we discuss what it is as a class, let’s read about it together.
• As we read, I want you to use this annotation key:
• Underline what you are going to do
• Highlight how you are going to do it
• Put a box around why we use DNA barcoding

DNA barcoding procedures

• Let’s review the procedures for collecting the samples

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• Here is the perimeter for collection we will use

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DNA barcoding procedures

1. Identify a plant for collection
2. Take a picture of the whole plant and one leaf
3. Collect one leaf
4. Take a tissue sample of leaf
5. Identify the species with a field guide and input your data

Proper Processing of Samples

Where to enter the data

• We are going to be using a shared excel document to keep the labeling of our samples consistent

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• https://docs.google.com/spreadsheets/d/1F8S8_mGmr-UKGfWTbahWljnEBPvLrFu7Wkrf93txXek/edit

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Now, let’s switch gears and get back into the current curriculum

Cancer-Causing

Genes

Cancer Causing Genes

Short video about Cancer Causing genes

Ted Ed - BRCA1

Agenda 11/30/16

Objective: Students will find and process a leaf tissue sample, identify its species, and record data about the leaf.

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• Find and Photograph Leaf/Plant (10 minutes)
• Tissue Sampling and Data Entry (30 minutes)

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Homework:

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• Due Friday 12/2 - Page 66 in your ISN

Agenda 11/30/16

1. Outside Goals (10 minutes)
1. Find a plant to identify
2. Remove one leaf and record location of plant ( Students A and B)
3. Photograph the plant and one leaf (Students C and D)
2. Inside Goals (30 minutes)
• Take a tissue sample from leaf
• Upload photos to Google Drive
• Locate plant on Google Maps
• Identify plant species with a field guide
• Record plant information on shared spreadsheet

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READ ALL DIRECTIONS CAREFULLY BEFORE YOU FOLLOW THEM

Inside Goals

Tissue Sample Station

Students A and B

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Gather materials

and follow station

marker carefully!

Data Entry Station

Students C and D

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Gather materials and follow station marker carefully!

Species Identification

Earliest Finishing Students

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Gather materials and follow station marker carefully!

FIRST

SECOND