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Laboratory Introduction

What is a protocol?

What is an enzyme?

Why do scientists �use protocols?

What are some examples �of enzymes in our lives?

Now let’s�practice�pipetting!

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Enzyme/Substrate Reactions�

Using Mushroom Tyrosinase

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What will happen?

Can it be prevented?

How?

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Enzymes are Proteins

DNA → RNA → Protein

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Organisms produce proteins, each having a different function…

Hormones

Hair and Nails

Structural Support

Enzymes

Muscle Contraction

Antibodies

Receptors, membrane channels

Nutrient Storage

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All living things produce enzymes…�

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Enzymes in our bodies have special jobs, too.

Digestion

DNA Replication

Metabolism

RNA Synthesis

Reproduction

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Enzymes are the Construction�Workers of the Cell…�

Enzymes help

BUILD UP or BREAK DOWN

molecular structures

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Enzymes are usually named with the suffix –ase added to the name of the substrate or reaction

Inhibitors�slow down�enzymes!

Paperclipase

Toothpickase

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Enzymes in our daily lives:

PROTEASE

HYDROLASE

LACTASE

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How do enzymes �speed up reactions?

The enzyme creates a favorable environment for the reaction.

Substrate

Enzyme

Substrate binds enzyme in active site.

Active site

Enzymes can be�used again and again!

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CROSSING A STEEP

MOUNTAIN ROAD

By FOOT

Time: Days

BIKING

Time: One Day

DRIVING

Time: Hours

Weak Catalyst

Strong Catalyst

Normal Rate

They increase the rate of a chemical reaction�by lowering the energy requirement.

Enzymes “catalyze” reactions

Chemical�Reaction:

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What happened?

Can it be prevented?

How?

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a series of reactions involving the enzyme tyrosinase

Enzymatic Browning

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Write one thing about enzymes that you can share with family or friends back in the classroom.

Remember to share �interesting facts about enzymes�with your classmates�during the lab!

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��

Tyrosinase is produced in

Bacteria • Fungi • Plants • Animals

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Tyrosinase & Albinism

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Heat-Sensitive Tyrosinase

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Mushroom Tyrosinase Lab�Protocol Overview

What’s a protocol again?

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STEP 1: Extract Enzyme

Tyrosinase

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STEP 2: Add Enzyme to Substrate

L-DOPA

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Tyrosinase + L-DOPA = Dopachrome (pigment)

STEP 3: Measure Product

Enzyme

Substrate

Product

+

=

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STEP 4: Graph Data Over Time

Amount

of product

Time

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TEMPERATURE

pH

INHIBITOR

STEP 5: Test Enzyme Activity �Under Different Conditions

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Summary of Protocol

  • Assign roles
  • Extract enzyme from mushroom
  • Add enzyme to substrate
  • Measure product
  • Test experimental conditions

- pH

- Temperature

- Inhibitor

  • Graph data over time
  • Compare treatments

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What happened?�

What did you do in this lab?

Let’s review the steps!

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STEP 1: Extract Enzyme

Tyrosinase

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STEP 2: Add Enzyme to Substrate

L-DOPA

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Tyrosinase + L-DOPA = Dopachrome (pigment)

STEP 3: Measure Product

Enzyme

Substrate

Product

+

=

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STEP 4: Graph Data Over Time

Amount

of product

Time

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STEP 5: Test Enzyme Activity �Under Different Conditions

TEMPERATURE

pH

INHIBITOR

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Laboratory Follow-up

Obstacles during the lab

Solutions

Give details!�What was hard and why?

How can we make this lab�easier to understand?

Now it’s time �for your proposals!