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DNA/RNA Set

Everything you need to teach with the MIT Edgerton Center DNA/RNA Set and DNA/RNA Booklet 1:

1. Curriculum Info and Ordering

2. Set Contents and Kit Management

3. Teacher Training

4. Lesson Preparation

5. Classroom Presentation

Models and lessons created by Kathleen M. Vandiver.

Graphics by Amanda Mayer. ©MIT. All Rights Reserved

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About the Slide Deck

Why a Slide Deck?

We chose to use this format because you can easily save the content to your Google Drive (File -> Make a Copy) and also print slides (File -> Download As or Print) for reference. If you already have a purchased your kit, we recommend you go to directly to Set Organization/Kit Management.

In this slide deck you’ll find everything you need to teach with the MIT Edgerton Center DNA/RNA Set and DNA/RNA Booklet 1: How to Use the Slide Deck

  • Slides 5-37 are info about ordering, curriculum, preparation and set-up, teacher tips, and additional resources. They are NOT for showing the class. Teachers may want to print a copy of the slides as ’how to’ notes for guidance during class.
  • Slides 38-67 are the Classroom Presentation (for leading the students during class). This lesson took 60 minutes with experienced instructors and focused students. Your lesson times may vary!
  • Slides 68-73 are the optional DNA replication activity. It is excellent for teaching semi-conservative replication.

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Distribution and Use

These materials are copyrighted by the Massachusetts Institute of Technology. You are free to copy and distribute our materials to teachers and students, provided that you acknowledge the source and retain any copyright statements on the materials.

Help others to find us by citing the MIT Edgerton Center website where much of the curriculum materials are available for download: https://edgerton.mit.edu/DNA-proteins-sets

These materials can be adapted by individual teachers for their classroom, but the alterations to the MIT Edgerton Center materials cannot be redistributed beyond your school or host organization.

Use of the MIT name: In your advertising materials, you may say that your program uses curriculum developed by the MIT Edgerton Center, and where appropriate, provide a link to our home page. You may not describe your program as a partnership or collaboration with MIT or the MIT Edgerton Center.

Select images and graphics in this slide deck are Public Domain and not under copyright.

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Table of Contents

(Click to jump to slide)

1. Curriculum Info and Ordering (slide 5)

2. Set Contents and Kit Management (slide 16)

3. Teacher Training (slide 21)

4. Lesson Preparation (slide 33)

5. Classroom Presentation (slide 38)

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Curriculum Info

and Ordering

DNA/RNA Set

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Teacher Testimonials

“Game changer. I feel more comfortable going deeper with middle school students.”

“Engaging and interesting. Will transform the way you teach/understand this topic.”

“Very tactile, excellent way to differentiate instruction to address different learning/ability levels of students.”

“Indispensable for developing a thorough, almost intuitive base of knowledge about this complex subject.”

Link to order form:

https://edgerton.mit.edu/mit-edgerton-center-classroom-sets-order-form

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An Innovation in Molecular Models

Today’s biology teachers need to teach about molecular function. This is hard to do! Students find it difficult to follow the many structural changes that molecules can undergo.

How the innovative MIT models help

Unlike most teaching aids, the MIT models are designed to teach what the molecules do, not just what molecules look like. These injection molded molecules are made to be manipulated. In this way, educators can teach abstract concepts in concrete ways.

Diverse populations including English language learners, middle schoolers, and AP biology students all have found the learning experience interesting and memorable.

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Appropriate for Multiple Instructional Levels

The models were designed for multiple levels of instruction: middle school, high school and the university/professional levels.

  1. In keeping with Universal Design, the booklets mini­mize the number of scientific terms so teachers can choose the scientific terms they will require students to learn. This gives all teachers flexibility and autonomy. Instructors can elaborate on the chemistry that is represented and choose appropriate vocabulary.

EXAMPLE: The model's universal design supports nucleotide chemistry at the university level with the 3' designation on the sugar to show directionality. The middle school teacher can also teach about the antiparallel nature of DNA, using the arrows on the model nucleotides.

  • The models provide flexibility for students with differ­ent learning needs. The models are an excellent means for teaching molecular biology to English language learners/multilingual learners (ELL/ML) because the concepts can be visually conveyed. Additionally, the models are ideal for tutoring. Instructors may wish to introduce support staff to the models.
  • While a wide age range is possible, we do not recommend the models for students under 11 years old. It is preferable for students to have mature motor skills and knowl­edge of cells and molecules before using the models.

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NGSS Performance Expectations

Grades 6-8:

MS-LS3-1

Develop and use a model to describe why structural changes to genes (mutations) located on chromosomes may affect proteins and may result in harmful, beneficial, or neutral effects to the structure and function of the organism.

Grades 9-12:

HS-LS1-1

Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.

HS-LS3-1

Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring.

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NGSS Cross-Cutting Concepts

Systems and System Models

Grades 6-8:

  • Models can be used to represent systems and their interactions—such as inputs, processes and outputs—and energy and matter flows within systems.
  • Models are limited in that they only represent certain aspects of the system under study.

Grades 9-12:

  • Models (e.g., physical, mathematical, computer models) can be used to simulate systems and interactions - including energy, matter, and information flows - within and between systems at different scales.
  • Models can be used to predict the behavior of a system, but these predictions have limited precision and reliability due to the assumptions and approximations inherent in models.

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NGSS Cross-Cutting Concepts

Structure and Function

Grades 6-8:

  • Structures can be designed to serve particular functions by taking into account properties of different materials, and how materials can be shaped and used.
  • Complex and microscopic structures and systems can be visualized, modeled, and used to describe how their function depends on the shapes, composition, and relationships among its parts, therefore complex natural structures/systems can be analyzed to determine how they function.

Grades 9-12:

  • Investigating or designing new systems or structures requires a detailed examination of the properties of different materials, the structures of different components, and connections of components to reveal its function and/or solve a problem.
  • The functions and properties of natural and designed objects and systems can be inferred from their overall structure, the way their components are shaped and used, and the molecular substructures of their various materials.

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Where can I order a set?

https://edgerton.mit.edu/mit-edgerton-center-classroom-sets-order-form

Please note that a classroom set consists of 14 kits for both the

Protein and the DNA curriculum. The classroom tRNA set consists of 4 kits.

We do not sell individual kits at this time.

Set Ordering

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Set Contents and Kit Management

DNA/RNA Set

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Set Contents

This Contents List is included with your DNA/RNA classroom set. Download a copy from our website.

Check the set using the Contents List to make sure nothing is missing.

DNA/RNA kit contents will be checked and maintained using the Kit Care Record (slide 19).

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Kit Preparations

There are 14 kits in the DNA/RNA Set:

  • Kits #1-12 are for student use
  • Kit #13 is for replacing parts as needed
  • Kit #14 is for teacher use in demonstrations

Prepare all kits before use:

1. Place strips of labeling or masking tape

numbered #1-14 between the two

latches on the side of each kit.

2. Number the top label on each kit #1-14

to match the strip on the side.

3. Print 14 double-sided copies of this file:

Team Kit Care Record

4. Fold the Team Kit Care Record papers into

quarters so page 1 faces out. Place one paper

inside each kit. Check off the kit type and write

the number of the kit on the paper.

2.

4.

3.

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How to Use the Kit Care Record

  1. Assign each team (2 students) to a kit # that they will always use.
  2. At the beginning of each lesson, give teams about 3 minutes to fill out the Team Kit Care Record. Initially explain that you expect teams to inspect kit pieces and confirm the piece counts each time they use the kit. Students should always report the kit status and not leave any blanks on the paper.
  3. The additional column on the paper for teacher initials is optional. Use this for teams that require your close supervision or for teacher notes/comments.
  4. Find a small box to be designated the “Lost & Found” for the whole class. Instruct the teams to place any extra pieces into the Lost & Found Box. Teams should also look for any missing pieces from their kit in the Lost & Found Box.

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Why is this Team Kit Care

System Effective?

?

  1. Teams keep the same kits and checking is only done at the beginning of each class. Each team is actually checking the previous team’s use.

  • The student kits were designed to minimize manual counting; the number of pieces in small compartments can be discerned at a glance. This repeated checking by the students decreases the work the teacher has to do in maintaining the kits.

  • The clear plastic box makes it possible for teachers to see the materials inside for quick visual confirmation.

  • The teacher won’t ever have to stop the class because one team is missing a piece. The check at the beginning of the class helps you know the lesson will be free of such disruptions.

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Teacher Training

DNA/RNA Set

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Teaching Essentials

Since our models are unique, they bring a unique set of challenges to the classroom and to modeling know-how! Please be attentive to the essential information that we will describe in detail:

  1. Practice with the hands-on models yourself beforehand so you will be a skillful and confident leader.
  2. Use the models at multiple levels to meet student needs.
  3. Teach proteins before DNA to increase student understanding.
  4. Prepare your kits and follow maintenance instructions using the Team Kit Care Record.

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Practice with the models

Our 1-hour teacher training video shows the entire DNA lesson being taught in a high school classroom.

Watch the DNA Set Teacher Training Video with DNA/RNA Booklet 1 and a kit.

Complete all the activities the way a student would:

  1. Follow the instructions in the presentation.
  2. Build the models yourself.
  3. Watch the videos to see exactly what to do for each simulation.
  4. Check yourself using the highlighted questions in the booklet.
  5. Take note of possible student problems.

Mastering the modeling yourself first is important. Students will be acting out the cell processes with the models to learn the steps, so they need to physically do it correctly to learn it correctly.

EXAMPLE: The two strands of the double helix are held together by weak hydrogen bonds and are easily separated and easily rejoined. With the right technique (as shown in the video) the model DNA strands should pop apart easily.

1.

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Use the models at multiple levels

  1. Teachers can differentiate the level of instruction for students’ learning needs.
  2. For example, to demonstrate the antiparallel nature of DNA, the model includes a 3' designation on the sugar. However, the teacher can use the nucleotides without referring to the 3' and instead use the arrows on the nucleotides to indicate the different directions of the DNA strands.
  3. The models are an excellent means for teaching molecular biology to English Language Learners (ELL) and Multilingual Learners (ML) because the concepts are visually conveyed. In the booklets, instructions are given in plain language with corresponding images.
  4. The models are ideal for tutoring. Instructors may wish to introduce their support staff in the use of the kits.
  5. For students who miss class, booklet pages can be assigned for make-up work with the kits.
  6. For student enrichment, additional activities in other DNA/RNA Booklets can be assigned.

2.

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Teach Proteins before DNA

Educators usually teach DNA first and then move on to teaching protein synthesis. This sequence mimics the central dogma sequence of molecular biology (DNA-> RNA-> protein) but...it makes the content more difficult to learn!

Proteins are unfamiliar molecules to most students who are learning protein synthesis. Thus, students are trying to learn about proteins and about protein synthesis at the same time. Teachers have also had difficulty teaching about proteins in the past because suitable models were unavailable. Our amino acids make the assembly and folding of protein chains into understandable and satisfying hands-on activities.

If you teach proteins before DNA, students will be able to focus on the process of synthesizing a protein from a gene. Students can anticipate that the amino acids will be joined into a long chain and they recognize that the order of the amino acids determines a protein's shape and function.

3.

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Prepare and Maintain the Kits

4.

If your classroom set is new, prepare your kits using slides 16-20.

If your classroom set has been used, check that the kits have been properly prepared:

  1. Kits should have masking tape numbered #1-14 between the two latches on the side of each kit.
  2. Each kit should have #1-14 on the top label to match the strip on the side.
  3. Check that each kit has a Team Kit Care Record.
  4. Check that the Team Kit Care Record faces out and has the number of the kit.

2.

4.

3.

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Booklet Tips

  1. Bold type in the booklet indicates an action for the students to do with the models.
  2. An underline in the text alerts the students to a new vocabulary word.
  3. A yellow highlighted question in the text is intended to have the students pause and reflect. The questions function as a periodic "check-in" for student understanding. Answers to these questions are in the back of booklet.
  4. Booklets should have both pages open and visible when in use.

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Lesson Presentation Tips

  1. The Classroom Presentation (slides 38-67) is designed for a teacher to guide a class through selected activities in DNA/RNA Booklet 1. This lesson took 60 minutes with experienced instructors and focused students. Your lesson times may vary!
  2. At times when students need 3D views to build the models correctly, the slides link to MIT Edgerton Center DNA videos.
  3. Text in blue bold print are the instructions for the teacher.
  4. The slides include booklet pages. This helps the class stay together and shows students what page they should be on during the lesson. Booklet page numbers are on the bottom right.
  5. The slides are NOT a substitute for your teams having their own booklets. Teams will need a booklet when working through an activity.
  6. The replication booklet pages and video have been omitted from this classroom presentation. It is an excellent activity for teaching semi-conservative replication. Use slides 68-73 to include this optional activity if desired.

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Additional Teaching Tips

General Tips:

  • Walk around the classroom to check the models for student understanding.
  • Give time for the students on a team to discuss the activities while building.
  • Ask students questions instead of giving the information yourself.

Specific Tips:

  • Page 6 - The base pairing activity is for students to experiment and figure out WHY certain bases pair together, NOT to demonstrate whether the students have memorized the base pairing rules.
  • Page 7 - Walk around the room during the ‘Building a Double Helix’ activity to check that all students’ DNA is antiparallel.
  • Page 9 - Make sure students practice opening DNA several times using the ‘pinch and pull up’ technique.
  • Page 24 - Students make a choice about base pairing the mRNA at the start of transcription to correct misconceptions. Keep reminding students that the mRNA sequence should be the same as the gene sequence.

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Short Instructional Videos for Students

These video links are included in the classroom presentation slides. The videos are useful for showing students before the lesson activity as part of the instructions or after as a short review.

MIT Edgerton Center DNA/RNA Set videos:

*Because DNA replication is an additional activity, this video is included with presentation slides 68-73.

Videos for other sets:

MIT Edgerton Center Protein Set videos

MIT Edgerton Center tRNA Set video

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Additional Resources

  1. The Cold Spring Harbor DNA Learning Center has great animations about cell processes, including transcription and translation.
  2. The DNA Glycosylase Power Point presentation is a great supplement to the DNA damage and repair exercises in DNA/RNA Booklet 2. The slides explain how DNA repair proteins prevent mutations from occurring by using a common example of 8-oxoguanine as the damaged nucleotide. Presenter notes are included in the PowerPoint slides. (This PowerPoint presentation was authored by Dr. Lourdes Aleman. Kindly credit the slides should you incorporate them into your own presentations.)
  3. The NASA DNA Resources for Educators is also about DNA damage and repair. Space travel exposes astronauts to high levels of DNA damaging radiation. The cells in the body can keep up with this increased damage, repairing the DNA, but only up to a point. When the DNA repair mechanisms fail, the cells either become cancerous or they kill themselves, neither of which is good for human health. Thus NASA is very interested in learning about DNA damage and repair processes to maintain human health.

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Summary/Review Questions

DNA Booklet 1 has questions (page 28) that cover the most important content of the lesson. We recommend you assign your students these questions for homework or go over them together in class.

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Lesson Preparation

DNA/RNA Set

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Recommended Room Set-Up

Requirements:

  1. 2 students per kit (3 if necessary)
  2. Students sharing a kit sit next to each other (not across from each other)
  3. Long tables are required for using the models and mats (at least 3’ long x 1.5’ wide)
  4. All students need to see the screen
  5. Have a table set up with your teacher kit and prepared models for classroom demonstrations

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Student Materials

Assign each team a kit/booklet number. Teams pick up the same numbered kit and booklet at the start of each class. Distribute other materials as needed during the lesson (codon cards and gene strips).

Required materials for each team (2 students):

  • 1 DNA/RNA Kit
  • 1 DNA/RNA Booklet 1
  • 1 Codon card pack
  • 1 Gene strip (There are 4 kinds: alpha, alpha mutated, beta, and beta mutated)*

*Make sure you have distributed at least 1 of each kind of gene strip.

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Teacher Materials

You will need a few models for teacher demonstrations. Build them and place them on your teacher demo table.

Build these 3 models prior to the lesson:

  • Make sure your strands are antiparallel.
  • Push both ends of the DNA towards each other before picking it up. (This is to make sure the phosphates and sugars are completely interlocked so it won’t fall apart.)
  • Pick it up and twist it to show the double helix.
  • Samples show nucleotide pairing options.
  • Keep samples ready for demo during base pairing activity.
  • Samples will help to clarify the definition of antiparallel DNA.

Incorrect pairs +

not antiparallel

DNA Double Strand with 12 Nucleotides

2 Potential Base Pair Configurations

Correct pairs +

antiparallel

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Presentation Tip Reminders

Review Before the Classroom Presentation:

Booklet Tips - slide 27

Lesson Presentation Tips - slide 28

Additional Teaching Tips - slide 29

The next slide starts the presentation for class.

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Classroom Presentation

DNA/RNA Set

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Teaching with the MIT Edgerton Center Models and Curriculum

PART 2: DNA�DNA/RNA Booklet 1 – including the Teacher Guide Commentary �July 30, 2021

Copyright MIT. All Rights Reserved. Funded in part by NIEHS P30‐ES002109

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40

Complete the Kit Care Record each time: name, date, and check all pieces

Students will need for this lesson:

  • DNA/RNA kit
  • DNA Booklet 1
  • Gene strip*
  • Codon Card pack

*Have students use their gene strip

to make sure they have enough space

to build their gene before starting the lesson!

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41

Review of Protein Structure

Before starting with DNA, let’s review the key features about protein structure!

  1. What are protein subunits called?
  2. What are the 3 parts of a protein subunit?
  3. Which part makes the subunits different?
  4. How do the subunits of proteins connect to each other?

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Please find and open DNA/RNA Booklet 1

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43

Explain the booklet instructions.

The text is formatted to help students – explain carefully.

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44

Demonstrate how to keep 2 booklet pages open at a time.

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45

RNA nucleotides are orange. For now, use only DNA nucleotides – no orange!

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46

Learn the parts of a nucleotide and look at differences between the bases.

Take out and compare the sizes of the 4 model bases. Which 2 bases are bigger? Why? Look at the chemistry for the differences.

Have all students build a 4 nucleotide DNA ladder. Encourage finding different ways a ladder could be built with the models! (This is not a test for the right base pairs.)

Ask for some different ways to make a ladder, then show some common results.

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47

Students discover the famous base pairing rule

(A-T and C-G) and why it works. They also realize that DNA is antiparallel.

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48

Play Video: Introduction to Nucleotides

Key Concepts in the Video:

1. Nucleotides are the building blocks of DNA

2. There are 4 DNA nucleotides: A, T, G, C

3. All nucleotides have 3 parts: sugar, phosphate, base

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49

Play Video: Building a DNA Double Helix

Key Concepts in the Video:

1. The DNA double helix is antiparallel.

2. Build the helix using the base pairing rules.

3. Open the model helix by pinching and pulling up.

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50

Now that you know the base pairing rules - build the double helix!

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51

Twist the DNA strands to make the double helix shape.

Push the ends towards each other first before pick up.

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52

Play Video: Opening DNA

Key Concepts in the Video:

1. Open the model helix by pinching and pulling up.

2. Hydrogen bonds hold the base pairs together.

3. Three reasons DNA needs to open regularly.

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53

Replication on slide 68

Practice pinching the DNA strands open several times.

This demonstrates hydrogen bonding between the bases.

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54

Define a gene for the class. Use the chromosome diagram to explain how DNA is organized in cells.

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55

Students construct genes on top of the strip!

Students have different gene strips - check 1 of each gene strip is being built (alpha, alpha mutated, beta, beta mutated)

Gene building and decoding video is next.

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Play Video: Decoding a Gene

Key Concepts in the Video:

1. A group of 3 nucleotides is a codon.

2. Each codon codes for an amino acid.

3. Use the Chart of DNA Codons to decode your gene strip.

IMPORTANT NOTE: The video says page 21 for the chart, but in the new booklet the Chart of DNA Codons is on page 17.

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57

Decode your gene using the Codon Cards.

Slide the Met card under the strip until

just the amino acid shows.

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58

Scan for each nucleotide triplet

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59

Different students have built different gene strips. Explain how the mutation in the beta mutated gene strip changed the protein sequence.

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60

Explain how the mutation in the alpha mutated gene strip did NOT change the protein sequence.

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61

Time for Transcription! Students already know:

  • The triplet code calls for a particular amino acid
  • The order of the amino acids in the DNA chain is important

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62

Introduce differences between DNA and RNA:

  • sugar
  • single stranded
  • U instead of T

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63

Which side (top or bottom) will you pair the RNA with?

(Do not advance slide until choice is made!)

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64

Base pair the RNA with the TOP strand!

Transcription video next!

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Play Video: Transcription

Key Concepts in the Video:

1. Pair mRNA with the strand opposite the gene.

2. T is replaced by U in RNA.

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66

Review where in the cell transcription is occurring and what will happen next to the RNA. What function does RNA serve?

Save the mRNA strand - we will use it for the next activity!

Take apart ONLY your DNA double strand.

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67

Writing out the answers to these questions in full sentences will create a summary of the key ideas.

Use the booklet to look up the answers on these pages.

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Additional Activity:

DNA Replication

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69

Play Video: Replication

Key Concepts in the Video:

1. DNA replication creates an exact copy of the DNA sequence.

2. DNA replication is semi-conservative.

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Back to slide 54

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End of Slide Deck

DNA/RNA Set