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Cannabis Extraction Solvent Safety – An Evidence Based Review

Introduction

1. OPENING STATEMENT

3. UNDERSTANDING DRINKING VS TRACE QUANTITIES

2. METABOLISM OF SOLVENTS

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Cannabis Home Sciences presented this slide show in January 2024. Here is a reduced version just focused on the metabolism of solvents for Gray Wolf’s Lair.

8. CONCLUSION

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Cannabis Extraction Solvent Safety – An Evidence Based Review

1. Opening Statement

The World Health Organization says there is “No level of alcohol consumption is safe for our health

Consumers ignore the significance of ethanol’s chemistry and the long term effects. Today, we will -

See Alcohol for what it is - a solvent

Learn each solvent’s Metabolic Pathways through the

liver & kidneys

See the difference between drinkable quantities and

residual traces through illustrated numbers

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Cannabis Extraction Solvent Safety – An Evidence Based Review

2. METABOLISM OF SOLVENTS

Review of Alcohol Metabolism

The liver metabolizes approximately 8g (+-2g) of ethanol per hour.

One ounce of 80 proof vodka has approximately 9g of Ethanol.

Ethanol Acetaldehyde

-> ->

CH3-CH2-OH CHO-CH3

Ethanol metabolizes into Acetaldehyde.

Acetaldehyde is both genotoxic, carcinogenic, and teratogenic, with Long term exposure

leading to Alcoholic Liver Disease, Cirrhosis of the liver, Cancer, Birth defects and more

Ethyl Acetate metabolizes into Ethanol thus shares this metabolic pathway.

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Cannabis Extraction Solvent Safety – An Evidence Based Review

2. METABOLISM OF SOLVENTS

Review of Alcohol Metabolism

Acetone, a ketone, is not broken down as it’s bio-friendly as-is, up to PDE levels.

Isopropyl metabolizes into Acetone thus shares Acetone’s metabolic pathway.

Isopropyl Acetone

-> ->

CH3-CHOH-CH3 CH3-2CO-CH3

Acetone is neither genotoxic, carcinogenic, or teratogenic. The Liver produces Acetone

ketones when breaking down fats. Acetone is used as an energy source during a low

carb state. Diabetic state of Ketosis has elevated acetone levels in the breath. Service

Dogs for diabetics trigger low sugar level alerts by smelling acetone.

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Cannabis Extraction Solvent Safety – An Evidence Based Review

3. UNDERSTANDING DRINKING VS TRACE QUANTITIES

Ethanol 3 Y 166.7mg 7.9g 336.1g 24.3 Y Y

*1 Partial GRAS rating for use in food

*2 FDA has published Acetone’s PDE to be higher than Ethanol’s PDE

*3 LD50 is an approximation based on rat studies and varies across Safety Data Sheets. 105lbs is 47.62kg

*4 Permanent visual damage may occur with minimum ingestion of 30 mL of methanol

Solvent Chemical Properties as Liquid

Ethyl Acetate 3 Y*1 117.0mg 5.5g 538.1g 6.0 N N

Acetone 3 Y*1 210.0mg*2 10.0g 276.1g 20.7 Y Y

Isopropyl 3 Y*1 138.0mg 6.5g 238.1g 18.2 Y Y

Methanol 2 N 30.0mg*3 6.5g 30.0g*4 32.7 N Y

Benzene 1 N 1.0mg 0.05g 157.4g 2.17 Y Y

Pentane 3 N 604.6mg 28.7g 276.1g 1.8 Y Y

Heptane 3 N 840.0mg 40.0g 238.1g 1.9 Y Y

Chem FDA GRAS FDA PDE FDA PDE LD50*3 Dialetric Forms Miscible

Name Class Rated mg/kg/day g/105lbs g/105lbs Constant Azeotrope w/water

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Cannabis Extraction Solvent Safety – An Evidence Based Review

3. UNDERSTANDING DRINKING VS TRACE QUANTITIES

How much is too much? The Solvent’s LD50 for a 105 lbs person

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Cannabis Extraction Solvent Safety – An Evidence Based Review

3. UNDERSTANDING DRINKING VS TRACE QUANTITIES

A: FDA allows 0.5% so 10mg * 0.5 = 0.05mg

A gummy weighing 4 grams..

has 10mg THC/CBD..

..with 0.5% solvent, which is 0.05ug

Permitted Daily Exposure (PDE) amount for a 105 lbs person in grams scaled to show micrograms.

Residual Solvent in 10mg = 50ug = 0.000.050ug

Acetone:........210.0 mg/kg/day = 9.996.000ug

Ethanol:........166.7 mg/kg/day = 7.901.000ug

Isopropyl:......138.0 mg/kg/day = 6.568.000ug

Ethyl Acetate:..117.0 mg/kg/day = 5.569.000ug

Heavier persons will have higher PDEs

The 10mg may safely contain up to 0.5% (0.05mg) residual solvent. This is well within the FDA’s PDE for all four solvents.

Q: How much solvent is in a 10mg gummy?

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Cannabis Extraction Solvent Safety – An Evidence Based Review

3. UNDERSTANDING DRINKING VS TRACE QUANTITIES

Ethanol’s Permitted Daily Exposure (PDE) limit for a 105 lbs person is 7.9g over 24 hours.

Ethanol PDE:...166.7 mg/kg/day = 7,901,000 ug

/ 24

Milligrams per hour = 329,208 ug

Residual solvent amount / 50 ug

=============

Gummies to be eaten every hour: 6,584

Q: How many 10mg gummies does it take

To reach the PDE’s 24hr limit?

A: 6,584 10mg gummies per Hour x 24 hrs!

(equivalent to 65 grams of Cannabis oil)

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Cannabis Extraction Solvent Safety – An Evidence Based Review

8. CONCLUSION

Here we’ve looked at four different solvents commonly found in the home. From a chemistry and biological perspective, these four represent the safest solvents that can be used without requiring special training or lab gear. This data reflects the current knowledge of the world medical community and might change in the future. A lot of the in-vivo solvent testing was on lab animals then extrapolated to human scale. These numbers may change with additional research.

On the consumer side, due to lack of in-depth understanding of chemistry, consumers believe that ethanol is the safest because it can be ingested. This seemingly logical conclusion permits users to disregard the dangers of long term exposures leading to cirrhosis of the liver, birth defects and DNA damage.

As shown in this discussion, the most feared solvents, acetone and isopropyl, viewed as poison at any level, are actually the least biologically harmful to the human body down at the trace level. By understanding these solvents at the molecular level and following through the metabolic pathways, the picture becomes clear. The most bio-friendly solvent is Acetone, being the least toxic of all 4 solvents at trace levels.

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Cannabis Extraction Solvent Safety – An Evidence Based Review

8. CONCLUSION

This fact is supported by the FDA establishing higher Permitted Daily Exposures for Acetone versus Ethanol. This is significant because the definition of PDE draws a line in the sand between harmless and harmful levels.

For virtually the entire world, the debate on Ethanol vs Isopropyl safety is actually a mute point because residual traces of these FDA Class 3 solvents are low enough to be safely metabolized by healthy liver and kidneys. When in compliance with regulations, residual solvents in edibles has been shown to be orders of magnitude under the FDA Permitted Daily limits.

Bringing it home:

  • Permitted Daily Exposure (PDE) is the dividing line between trace levels and drinking levels.
  • You are never going to encounter drinking quantities of solvents by ingesting edibles.
  • Drinking exposures are often over PDE values. One shot of 80 proof Vodka has 9.4 grams ethanol, more than the PDE for a 105lbs person.
  • Traces of solvent in edibles are orders of magnitude below the EPA’s PDE values.

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Cannabis Extraction Solvent Safety – An Evidence Based Review

8. CONCLUSION

In closing, when permitted by local laws, medical patients at home have good, safe, options for solvent selection. Some Chemical-Intolerant health issues might preclude certain solvents based individual sensitivities. Those suffering from impaired liver or kidney function may now choose which solvent best fit their chemical sensitivity. This premise needs to be discussed with medical professionals experts in the field of Toxicology.

Link to all references

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Cannabis Extraction Solvent Safety – An Evidence Based Review

Definitions

Glossary of Definitions Given by Many Gov’t Agencies (EPA,FDA,CDC,etc)

PDE Permitted Daily Exposure values are calculated based on NOAEL & LOAEL

NOAEL / LOAEL physical measurements - For in-vivo Bioanalytical chemistry measurements

NOAEL No Observable Adverse Effects Level - Limit of detectability. Undetectable below this level

LOAEL Lowest Observable Adverse Effects Level -

The highest dose level that does not produce a significant increase in adverse effects

LOD / LOQ theoretical measurements - For in-vitro Analytical Chemistry modeling - Theoretical LOAEL/NOAEL

LOD Limit of Detection noise floor - Less reliable. Can't always be measured

LOQ Limit of Quantification - More reliable. Consistently measurable

LD50 Lethal Dose for 50% of those affected

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Cannabis Extraction Solvent Safety – An Evidence Based Review

Definitions

OSHA Definitions for Airborne hazzards

PEL Permitted Exposure Limit of chemical exposure of airborne contaminants

For vapor fire hazards

LEL Lowest Explosive Limit - The National Fire Protection Agency (NFPA)

UEL Upper Explosive Limit - The National Fire Protection Agency (NFPA)

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