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3.3 Meiosis

3.3.U3 DNA is replicated before meiosis so that all chromosomes consist of two sister chromatids.

The homologous chromosomes associate with each other to form bivalents (synapsis).

Sister Chromatids

Synapsis

Centromere

Homologous Chromosomes

Kinetochore

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The micrographs above show the formation of bivalents (left) and the segregation caused by both anaphase I and II (right). These possesses combined with crossing over and random orientation ensure a near infinite variation of genetic information between gametes.

3.3 Meiosis

Alleles segregate during meiosis allowing new combinations to be formed by the fusion of gametes.

Essential idea:

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Statement

Guidance

3.3.U1

One diploid nucleus divides by meiosis to produce four haploid nuclei.

3.3.U2

The halving of the chromosome number allows a sexual life cycle with fusion of gametes.

3.3.U3

DNA is replicated before meiosis so that all chromosomes consist of two sister chromatids.

3.3.U4

The early stages of meiosis involve pairing of homologous chromosomes and crossing over followed by condensation.

The process of chiasmata formation need not be explained.

3.3.U5

Orientation of pairs of homologous chromosomes prior to separation is random.

3.3.U6

Separation of pairs of homologous chromosomes in the first division of meiosis halves the chromosome number.

3.3.U7

Crossing over and random orientation promotes genetic variation.

3.3.U8

Fusion of gametes from different parents promotes genetic variation.

3.3 Meiosis

Understandings

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Statement

Guidance

3.3.A1

Non-disjunction can cause Down syndrome and other chromosome abnormalities.

3.3.A2

Studies showing age of parents influences chances of non-disjunction.

3.3.A3

Description of methods used to obtain cells for karyotype analysis e.g. chorionic villus sampling and amniocentesis and the associated risks.

3.3.S1

Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells.

Drawings of the stages of meiosis do not need to include chiasmata. Preparation of microscope slides showing meiosis is challenging and permanent slides should be available in case no cells in meiosis are visible in temporary mounts.

3.3 Meiosis

Applications and Skills

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3.3 Meiosis

Vocabulary

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P

M

A

T

3.3 Meiosis

Review: 1.6.U1 Mitosis is division of the nucleus into two genetically identical daughter nuclei.

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Use the animated tutorials to learn about mitosis

3.3 Meiosis

Review: 1.6.U1 Mitosis is division of the nucleus into two genetically identical daughter nuclei.

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1876 - German biologist Oscar Hertwig recognized the role of the cell nucleus during inheritance and chromosome reduction during meiosis from work on Sea Urchins.

1883 - Belgian zoologist Edouard Van Beneden discovered in the roundworm Ascaris how chromosomes organized meiosis (the production of gametes).

1890 - The significance of meiosis for reproduction and inheritance was first described by German biologist August Weismann who noted that two cell divisions were necessary to transform one diploid cell into four haploid cells.

In the 19th century was very difficult to observe the behaviour of chromosomes in cell: the choice of organism and tissue, slide preparation and interpreting microscope images are all difficult to do successfully. It therefore it took years of careful examination by Scientists to discover and fully understand meiosis.

3.3 Meiosis

The History of Meiosis

Nature of Science: Making careful observations—meiosis was discovered by microscope examination of dividing germ-line cells. (1.8)

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3.3 Meiosis

Meiosis is a reduction division of the nucleus to form haploid gametes

3.3.U1 One diploid nucleus divides by meiosis to produce four haploid nuclei.

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3.3 Meiosis

Meiosis is a reduction division of the nucleus to form haploid gametes

3.3.U1 One diploid nucleus divides by meiosis to produce four haploid nuclei.

Homologous chromosomes each have the same genes in the same order, but there may be variation between them, resulting in different alleles.

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Meiosis is a reduction division of the nucleus to form haploid gametes

Second division of the nucleus

3.3 Meiosis

What is meiosis?

3.3.U1 One diploid nucleus divides by meiosis to produce four haploid nuclei.

It results in cells with half the number of chromosomes ( hence reducing)

Occurs in sexual reproduction in the synthesis of gametes

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The animations are a great way to visualise the process – watch and take notes.

3.3 Meiosis

Meosis is a reduction division of the nucleus to form haploid gametes

3.3.U1 One diploid nucleus divides by meiosis to produce four haploid nuclei.

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3.3 Meiosis

Outline the differences between the behaviour of chromosomes in Mitosis and Meiosis (5 marks)

Mitosis

Meiosis

One division

Two divisions

Diploid cells produced

Haploid gametes produced

No crossing-over in prophase

Crossing-over in prophase I

No chiasmata formation

Chiasmata form

Homologous pairs do not associate and line up at the equator in metaphase

Homologous pairs associate as bivalents and lined up at the equator in metaphase I

Sister chromatids separate in anaphase

Homologous pairs separate in anaphase I

Sister chromatids separate in anaphase II

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3.3 Meiosis

3.3.U3 DNA is replicated before meiosis so that all chromosomes consist of two sister chromatids.

The homologous chromosomes associate with each other to form bivalents (synapsis).

Sister Chromatids

Synapsis

Centromere

Homologous Chromosomes

Kinetochore

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Crossing-over between non-sister chromatids can take place. This results in recombination of alleles and is a source of genetic variation in gametes.

3.3 Meiosis

Prophase I

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells. AND 3.3.U4 The early stages of meiosis involve pairing of homologous chromosomes and crossing over followed by condensation.

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3.3 Meiosis

Prophase I

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells. AND 3.3.U4 The early stages of meiosis involve pairing of homologous chromosomes and crossing over followed by condensation.

Plural: Chiasmata

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Random assortment occurs when each bivalent aligns independently and hence the daughter nuclei get a different mix of chromosomes.

The bivalents line up at the equator.

3.3 Meiosis

Metaphase I

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells. AND 3.3.U5 Orientation of pairs of homologous chromosomes prior to separation is random.

This is a significant source of genetic variation: there are 2n possible orientations in metaphase I and II. That is 223 in humans – or 8,388,068 different combinations in gametes!

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  • Nuclear membranes dissolves
  • Chromosomes, consisting of two sister chromatids, condense
  • No crossing-over occurs.
  • centrioles move to opposite poles (perpendicular to previous

3.3 Meiosis

Prophase II

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells.

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Spindle fibres form and attach at the centromeres.

Pairs of sister chromatids align at the equator.

3.3 Meiosis

Metaphase II

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells.

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Four new haploid nuclei are formed.

  • Nuclear membrane reforms
  • Chromosomes decondense
  • Cytokinesis begins, dividing the cells.
  • The end result of meiosis is four haploid gamete cells.
  • Fertilisation of these haploid gametes will produce a diploid zygote.

3.3 Meiosis

Telophase II

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells.

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3.3 Meiosis

Telophase II

3.3.S1 Drawing diagrams to show the stages of meiosis resulting in the formation of four haploid cells.

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Prophase I

Metaphase I

Random orientation of the homologous chromosomes means there are 2n possible orientations in metaphase I and II. That is 223 in humans – or 8,388,068 different combinations in gametes!

Metaphase II

3.3 Meiosis

Because both crossing-over and random orientation occur during meiosis the result is is effectively infinite genetic variation in the haploid gamete.

3.3.U7 Crossing over and random orientation promotes genetic variation.

Crossing-over between non-sister chromatids results in recombination of alleles

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Increased genetic variation produces a more resilient population that is more likely to withstand environmental change such as a disease. Genetic variation is essential for successful change by evolution.

Meiosis in a single individual produces near infinite variation, but genetic variation is further increased by:

  • Meiosis occurs in two individuals
  • Alleles from two organisms combine in novel ways

3.3 Meiosis

For a new organism to arise sexually meiosis must occur in both parents followed by fusion of the gametes (fertilisation)

3.3.U8 Fusion of gametes from different parents promotes genetic variation.

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3.3 Meiosis

Sexual reproduction involves fertilisation, the fusion of gametes (sex cells), one from each parent.

3.3.U2 The halving of the chromosome number allows a sexual life cycle with fusion of gametes.

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Metaphase I

Anaphase I

Anaphase II

Metaphase II

*Disjunction is the term used to describe the seperation of chromosomes

For example homologous chromosomes can fail to separate at anaphase. This is called non-disjunction.

Normal division

Disjunction in Meiosis I

Disjunction in Meiosis II

3.3 Meiosis

Meiosis like all processes is sometimes subject to mistakes.

3.3.A1 Non-disjunction can cause Down syndrome and other chromosome abnormalities.

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  • If the gamete is involved in fertilization, the result will be an organism with too few or too many chromosomes.
  • Trisomy is the term used to describe organisms with one extra chromosome (three of one chromosome instead of pair)
  • Monosomy is the term used to describe organisms with one extra chromosome (three of one chromosome instead of pair)
  • Non-disjunction of many human chromosomes is so serious that either the zygote is not viable or the individual does not survive.
  • Trisomy 21 is commonly known as Down Syndrome. The individual possesses three of chromosome 21 and 47 chromosomes in total

3.3 Meiosis

3.3.A1 Non-disjunction can cause Down syndrome and other chromosome abnormalities.

The result of non-disjunction is gametes that either have one chromosome too many or one too few.

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