Configs that have at least 1 SME review, but prefer to have at least 2-3 more reviewers

1. alamarBlue assay
• Dye-based plate reader assay, measures cell viability, proliferation, cytotoxicity

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• Chromogenic and Fluorescence In Situ Hybridization
• Colorimetric or fluorescent probes hybridized to target sequences, measures genomic elements and chromosome mapping and abnormalities

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• Chromosome Aberration
• Uses Giemsa stain, FISH, or CISH to measure chromosome aberrations (from DSBs etc)

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• In Vivo Calcium Imaging
• Current data set is neuro-focused: injection of viral construct with calcium indicators into brain, craniotomy to install fiber optic camera or window, which allows real-time measurement of neural activity during behavior assays

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• Continuous Glucose Monitoring
• Monitor that continuously measures subject glucose levels, e.g. Freestyle Libre

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• Autoshaping/Operant Response
• Mammalian (mostly rodent) learning/reinforcement behavior assay
• Likely hosting a subgroup meeting to review this config

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Configs still needing SMEs

• Clinical Chemistry Analysis
• Clinical hematological and biochemical analysis
• Spectrophotometry for Microbiology
• Hexoskin/Astroskin
• Wearable vitals monitoring device, ECG/Accelerometer-based
• Very specific and niche assay, not expecting many SMEs
• Microindentation/Nanoindentation
• Probe used to measure the hardness/mechanical properties of surfaces (e.g. tissues, cells, coatings, etc)
• Finite Element Analysis
• Modeling-based assay to analyze composite materials
• Nanoparticle Tracking Analysis
• Microscopy assay, uses light scattering to record nanoparticle movement in medium

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How to provide feedback

• OSDR Public Config List (bottom of the first sheet has the configs needing SME review)
• Can also send feedback in any format (email, or just put comments anywhere in the Google Sheet docs)

Bioluminescence Imaging (Draft in Progress)

• Bioluminescence, Fluorescence, and X-ray imaging

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• Multi-spectral, multi-wavelength imager

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• Visualize multiple fluorescent tags in vivo

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• 3D constructed images

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• Used commonly to track tumor progression non-invasively

Label-free impedance-based cell assay (Draft in Progress)

Schematic image of electron flow in a single well of an E-Plate. A) Electron flow from one biosensor to the other through culture media only. B) Impeded electron flow from one biosensor to the other due to cells attached/growing on gold microelectrodes

Zoomed-in photograph of gold microeletrodes on the E-Plate® well bottom.

D) Unstained human cells, growing on microelectrodes

Unlabeled cells in culture adhere to the well/electrodes and impede the electron flow. After treatment is applied and apoptosis is induced, cells begin to detach. The subsequent change in impedance is a measure of real-time cell death.

xCELLigence real-time cytolysis assay