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Alkaloids and Terpenoid’s are Secondary metabolites

Primary Metabolite Secondary Metabolites

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1) Primary metabolites are essential for life These are non-essential. process. (Growth of the cells)

2)These are identified universally, throughout the source.

These are seldom, like in some portions of the source, like steam, bark, etc.

3) These are produced continuously during the growth phase and are involved in primary metabolic processes such as respiration and photosynthesis, etc

These compounds do not have a continuous production. However they are produced during non-growth phase of cells. (Synthetically).

4) Heavy mol.wt compounds.

Low mol.wt compounds.

5) Structure cannot be drawn.(Undefined)

Can be drawn. (Defined)

6) DNA, Proteins, carbohydrates, and lipids are the main primary metabolites.

Alkaloids, Terpenoid, phenolics, sterols, steroids, essential oils and lignins, etc

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Natural organic compounds.

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Components of a variety of fruit and floral flavors and aromas.

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Used in perfumes, essential oils and medicines.

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Terpenes are unsaturated compounds formed by joining together isoprene units.

What are Terpenes?

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The name "terpene" is derived from the word "turpentine"

Terpenoids - oxygen-containing terpenes (alcohols, ketones, aldehydes)

rose oil

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The basic molecular formulae of terpenes are multiples of that, (C5H8)n where n is the number of linked isoprene -Isoprene - 2-methyl-1,3-butadiene- units. This is called the isoprene rule or the C5 rule.

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Compounds containing carbon atoms in multiples of 5 suggest a C5 building block - isoprene units linked in a “head-to-tail” fashion.

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Biogenesis or Biosynthesis of Terpenes :-

  • Terpenes, steroids, lipids are synthesised

from acet:yl-coenzynte A -the biological building blockr-------..

  • Acetyl-coenzyme A - acyl halide analogy

- Acyl substitution occurs

Acctyt -CoA

Acyl halide

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Biosynthesis starts from Pyruvic acid.

Pyruvic acid

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RULE - isopentenyl pyrophosphate and dimethylallyl pyrophosphate are the C5 building blocks for the biosynthesis of terpenes

Elimination of a proton from a carbocation intermediate generates a double bond (E1 reaction)

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Geranyl pyrophosphate - C10 building block for many biological monoterpenes

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Isopentenyl pyrophosphate is only one of the necessary reagents for the biosynthesis of terpenes… dimethylallyl pyrophosphate, a C5 isomer, is also necessary:

Add. of H+

elimination of a proton

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“Head” - branched end of isoprene

“Tail” - unbranched end of isoprene

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Finding the isoprene building block - cyclic compounds

2-methyl-1,3-butadiene

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Finding the isoprene building block - sesquiterpenes (C15)

Finding the isoprene building block - triterpenes (C30) -

Squalene has a natural and vital part in the synthesis of all plant and animal sterols, including cholesterol, steroid hormones, and vitamin D in the human body

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Finding the isoprene building block - tetraterpenes (C40)

Lycopene's eleven conjugated double bonds give it its deep red color and are responsible for its antioxidant activity.

Lycopene is responsible for the red color in tomatoes and watermelon

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β-carotene is the compound that causes carrots and apricots to be orange

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Question : Which Terpene ?

A) Mono 2) Di 3) Sesqui 4) Sester

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Humulene

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found in the essential oils of Humulus lupulus

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Limonene

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(skin of citrus fru its)

Menthol

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(peppermint)

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H3C '/CH3

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Camphor (Camphor tree)

3 isoprene units

15 carbon atoms

a-Selinene

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Pinene, terpe---, L-menthol, camphor, amyrene, geraniol, myrcene, Nerol, Carvone, Lanosterol, Cholesterol, farnesol, Artimesnin,

THC (teteahydrocannabinol), Vitamin A & D derivatives, etc

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Question: Biosynthetic pathway of Cholesterol (Steroid)?

•Shikimic acid pathway

•Mevalonic acid pathway

•Acetate-melanoate pathway

•None

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NOTE: All steroid moiety contain natural products are bio- synthesied by Mevalonic acid pathway

Please Check for the key words below and add ‘’Terpenoids’’ as Cpd class

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Questions

  • Which unit makes up every terpene?

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  • How many carbons are there in an isoprene unit?

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  • What is the systematic name for isoprene?

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  • What is an oxidised terpene known as?

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Answers

Which unit makes up every terpene?

Isoprene unit

How many carbons there are in an isoprene unit?

Five

What is the systematic name for isoprene?

2-methylbuta-1,3-diene

What is an oxidised terpene known as?

Terpenoid

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