Learning Resources for iGEM

2020-2021 iGEM Board

I stole this theme from JHU baja. Lol

Notes

The “Essential Procedure Review” includes the most important techniques. If you are confused about some of the topics, please reference the “Biology and SynBio Review”.

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This is by no means comprehensive. For a more thorough resource list, please reference “Learning & Bioinformatics Resources for iGEM” in the JHU iGEM team Google Drive or at this link.

Essential Procedure Reviews

• Gel Electrophoresis
• Streak Plate
• Restriction Digest
• Plating Antibiotic over Agar
• Pouring Agar Plates
• Bacterial Transformation
• Primer Design
• PCR
• Gel Electrophoresis

Procedure Review

Addgene - Restriction Cloning

Addgene - Gibson Assembly and Other Long-Homology Based Cloning Methods

Addgene - How to Verify Your Plasmid

Addgene - Optimizing Plasmid Yields

Khan Academy Medicine - Gel Electrophoresis

Biology & SynBio Review

• Concepts of Biology Ch. 9
• Concepts of Biology Ch. 10
• Molecular Biology Reference
• Plasmids 101

Further SynBio Review

• Addgene - Inducible Promoters
• Addgene - Repressible Promoters
• Addgene - Positive and Negative Selection
• Addgene - Transformation, Transduction, Bacterial Conjugation, and Transfection
• Addgene - Protein Expression
• Addgene - E. Coli Strains for Protein Expression
• Addgene - Common Lab E. Coli Strains
• Addgene - Plasmid Incompatibility
• Addgene - Multicistronic Vectors
• Addgene - Protein Tags
• Addgene - Methylation and Restriction Enzymes

Basic Bioinformatics Tools

• NCBI BLAST
• https://www.youtube.com/watch?v=HXEpBnUbAMo (OLD tutorial)
• https://blast.ncbi.nlm.nih.gov/Blast.cgi
• NCBI RefSeq and PDB
• https://www.youtube.com/watch?v=QIZ8QH6JcC8 (tutorial)
• https://www.ncbi.nlm.nih.gov/
• If you are interested in learning more about Bioinformatics, JHU Coursera has the following tracks (with certificates available if you sign up for Coursera using your @jhu.edu email)
• Data Science
• Genomic Data Science

Other Resources

• Learning & Bioinformatics Resources for iGEM
• OpenWetWare - Digital Tools and Resources
• Comprehensive list