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11.1 Antibody Production and Vaccination

Immunity is based on recognition of self and destruction of foreign material.

Essential idea:

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11.1 Antibody Production and Vaccinations

Vocabulary

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11.1 Antibody Production and Vaccination

Syllabus Reference

Statement

Guidance

11.1.U1

Every organism has unique molecules on the surface of its cells.

11.1.U2

Pathogens can be species-specific although others can cross species barriers.

11.1.U3

B lymphocytes are activated by T lymphocytes in mammals.

Limit the immune response to mammals.

11.1.U4

Activated B cells multiply to form clones of plasma cells and memory cells.

11.1.U5

Plasma cells secrete antibodies.

11.1.U6

Antibodies aid the destruction of pathogens.

11.1.U7

White cells release histamine in response to allergens.

11.1.U8

Histamines cause allergic symptoms.

11.1.U9

Immunity depends upon the persistence of memory cells.

11.1.U10

Vaccines contain antigens that trigger immunity but do not cause the disease.

11.1.U11

Fusion of a tumour cell with an antibody-producing plasma cell creates a hybridoma cell.

11.1.U12

Monoclonal antibodies are produced by hybridoma cells.

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11.1 Antibody Production and Vaccination

Syllabus Reference

Statement

Guidance

11.1.A1

Smallpox was the first infectious disease of humans to have been eradicated by vaccination.

11.1.A2

Monoclonal antibodies to HCG are used in pregnancy test kits.

11.1.A3

Antigens on the surface of red blood cells stimulate antibody production in a person with a different blood group.

11.1.S1

Analysis of epidemiological data related to vaccination programmes.

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11.1 Antibody Production and Vaccinations

Stick these on other people

Do Now

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  • All nucleated cells of the body possess unique and distinctive surface molecules that identify it as self
  • Major histocompatibility complex molecules (MHC class I

11.1 Antibody Production and Vaccinations

Identifying as self

11.1.U1 Every organism has unique molecules on the surface of its cells.

(MHC class I)

(MHC class I)

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  • All nucleated cells of the body possess unique and distinctive surface molecules that identify it as self
  • These self markers are called major histocompatibility complex molecules (MHC class I) and function as identification tags

11.1 Antibody Production and Vaccination

11.1.U1 Every organism has unique molecules on the surface of its cells.

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  • Any substance that is recognised as foreign and is capable of triggering an immune response is called an antigen (non self).

                  • Lymphocytes trigger antibody production (adaptive immunity)

11.1 Antibody Production and Vaccinations

Identifying foreign or non-self

11.1.U1 Every organism has unique molecules on the surface of its cells.

foreign

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Covid-19

11.1 Antibody Production and Vaccinations

So what is the difference between these 2 pathogens?

11.1.U1 Every organism has unique molecules on the surface of its cells.

Influenza

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11.1 Antibody Production and Vaccination

11.1.U1 Every organism has unique molecules on the surface of its cells.

The surface of an organisms’ cells are covered in uniquely shaped molecules, examples include:

  • the polysaccharides of a bacterium’s cell wall
  • the glycoproteins embedded in the plasma membrane of a eukaryote
  • The protein coat (capsid) of a virus also contains uniquely shaped molecules.

Unfamiliar surface molecules that cause the production of antibodies are called antigens.

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Hemagglutinin allows the virus to stick to the host cells

11.1 Antibody Production and Vaccination

11.1.U1 Every organism has unique molecules on the surface of its cells.

Neuraminidase helps with the release of the newly-formed virus particles

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11.1 Antibody Production and Vaccination

11.1.U1 Every organism has unique molecules on the surface of its cells.

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This means that red blood cells can be transferred between individuals without automatically causing immune rejection

11.1 Antibody Production and Vaccination

What is so special about red blood cells?

11.1.U1 Every organism has unique molecules on the surface of its cells.

Red blood cells are not nucleated and hence do not possess the same distinctive and unique self markers as all other body cells

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11.1 Antibody Production and Vaccination

11.1.A3 Antigens on the surface of red blood cells stimulate antibody production in a person with a different blood group.

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11.1 Antibody Production and Vaccination

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11.1 Antibody Production and Vaccination

11.1.U2 Pathogens can be species-specific although others can cross species barriers.

  1. Touch (direct contact)
  2. Sneezing/coughing (through the air)
  3. Sharing injections
  4. In food
  5. Kissing
  6. Sexual intercourse
  7. Blood transfusions
  8. Bad sanitation
  9. Animals

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11.1 Antibody Production and Vaccination

Make a list of as many diseases spread by animals to humans.

11.1.U2 Pathogens can be species-specific although others can cross species barriers.

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Passive – injected manmade antibodies or maternal antibodies

11.1 Antibody Production and Vaccination

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

Active – Exposure to pathogen or vaccination

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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  • Helper T cells:
  • help with the activation of B cells, and other immune cells.
  • Formed in the bone marrow and mature in the thymus
  • T cells can only bind to MHC’s not directly to antigens

11.1 Antibody Production and Vaccination

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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  • Cytotoxic T cells are responsible for removing pathogens and infected host cells.

11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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  • Activated by cytokines released from Helper T Cells
  • When a B cell encounters an antigen that is complementary or matches its membrane-bound antibody, it quickly divides in order to become either a memory B cell or an effector (plasma cell) B cell,
  • Antibodies can bind to antigens directly.

11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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11.1 Antibody Production and Vaccination

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

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a. specific immune response/antibody production as a consequence of the presence of bacterial antigens �b. macrophage / phagocyte ingests bacterial pathogen displaying bacterial antigens on surface

c. attached to major histocompatibility/MHC molecules

d. helper T cell activated by presentation of antigen on surface of macrophage

e. activated helper T cell binds to B cell specific to the antigen

f. stimulated B cell undergoes repeated mitotic/cell divisions

g. «cells enlarge and differentiate» to form clone of plasma cells

h. plasma cells produce specific antibodies

i. antibodies bind to bacteria making them easier to digest by white cells OR opsonization OR agglutination

j. (some antibodies combined with antigen) activate a complement cascade to kill bacteria directly

k. some antibodies act as antitoxins / neutralize toxins / change chemical structure of toxins

l. once begun, antibody production lasts for several days until all antigens destroyed

m. memory cells remain in blood giving extended immunity

11.1 Antibody Production and Vaccination

Explain the production and role of antibodies in defense against bacterial pathogens in humans.(8)

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

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m. memory cells remain in blood giving extended immunity

11.1 Antibody Production and Vaccination

Explain the production and role of antibodies in defense against bacterial pathogens in humans.(8)

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

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11.1 Antibody Production and Vaccination

11.1.U3 B lymphocytes are activated by T lymphocytes in mammals.

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11.1 Antibody Production and Vaccination

11.1.U4 Activated B cells multiply to form clones of plasma cells and memory cells. 11.1.U5 Plasma cells secrete antibodies.

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11.1 Antibody Production and Vaccination

11.1.U9 Immunity depends upon the persistence of memory cells.

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Antibodies (aka immunoglobulins) are large, Y-shaped protein produced mainly by plasma cells.

11.1 Antibody Production and Vaccination

11.1.U6 Antibodies aid the destruction of pathogens.

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11.1 Antibody Production and Vaccination

11.1.U6 Antibodies aid the destruction of pathogens.

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  • Precipitation – Soluble pathogens become insoluble and precipitate 
  • Agglutination – Cellular pathogens become clumped for easier  removal 
  • Neutralisation – Antibodies may occlude pathogenic regions (e.g. exotoxins)
  • Inflammation – Antibodies may trigger an inflammatory response within the body
  • Complement activation – Complement proteins perforate membranes (cell lysis)

11.1 Antibody Production and Vaccination

What can antibodies do?

11.1.U6 Antibodies aid the destruction of pathogens.

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11.1 Antibody Production and Vaccination

What can antibodies do?

11.1.U6 Antibodies aid the destruction of pathogens.

  • Neutralisation – attachment stops toxins from affecting or entering cells, viruses from docking/invading cells, and bacteria from efficiently functioning and therefore attacking cells

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11.1 Antibody Production and Vaccination

What can antibodies do?

11.1.U6 Antibodies aid the destruction of pathogens.

  • Opsonization – through attachment antibodies mark the pathogens making them easily identifiable by other immune cells, e.g. so macrophages can find and engulf and digest them

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11.1 Antibody Production and Vaccination

What can antibodies do?

11.1.U6 Antibodies aid the destruction of pathogens.

  • Agglutination – antibodies attach to each other causing a clumping of the pathogen (enhancing the effects of neutralisation and opsonization)

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11.1 Antibody Production and Vaccination

What can antibodies do?

11.1.U6 Antibodies aid the destruction of pathogens.

  • Complement activation – antibodies ‘encourage’ other components such as complement proteins in the blood plasma form a membrane attack complex that destroys the cell membrane in the pathogen by allowing water and ions inside the membrane i.e. lysing the cell

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An allergen is an environmental substance that triggers an immune response despite not being intrinsically harmful

11.1 Antibody Production and Vaccination

11.1.U7 White cells release histamine in response to allergens.

Redness, heat, swelling and localised pain are all typical symptoms of an allergic response

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  • Allergen enters the body
  • Triggers B Cells to produce antibodies (specifically IgE)
  • Antibody (IgE) binds to Mast Cells
  • Mast cells release Histamine
  • Histamine triggers an allergic reaction

11.1 Antibody Production and Vaccination

11.1.U7 White cells release histamine in response to allergens.

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Key Words: Allergen, Mast Cells, IGE antibody, Histamine

11.1 Antibody Production and Vaccination

What happens to Mr Trent when he eats shrimp?

11.1.U7 White cells release histamine in response to allergens.

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  • Key Words: Allergen, Mast Cells, iGE antibody, Allergic Reaction (Inflammatory Response)

11.1 Antibody Production and Vaccination

What happens to Mr Trent the next time he eats shrimp?

11.1.U7 White cells release histamine in response to allergens.

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  • Capillaries Widen
  • Increased Permeability
  • White Blood Cells Increase
  • Immune Response

11.1 Antibody Production and Vaccination

11.1.U7 White cells release histamine in response to allergens. 11.1.U8 Histamines cause allergic symptoms.

  • Increased Blood Flow
  • Fluid moves into tissues
  • White blood cells fight and leak out of blood vessel
  • Fever and plasma cells
  • Heat and Redness
  • Swelling/Loss of Movement
  • Tenderness
  • Pain

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• Breathing: wheezing, shortness of breath, throat tightness, cough, hoarse voice, chest pain/tightness, trouble swallowing, itchy mouth/throat, nasal stuffiness/congestion�• Circulation: pale/blue color, low pulse, dizziness, lightheadedness/passing out, low blood pressure, shock, loss of consciousness�• Skin: hives, swelling, itch, warmth, redness, rash�• Stomach: nausea, pain/cramps, vomiting, diarrhea�• Other: anxiety, feeling of impending doom, itchy/red/watery eyes, headache, cramping of the uterus

11.1 Antibody Production and Vaccination

Watch this and identify the symptoms of anaphylaxis may include:

11.1.U7 White cells release histamine in response to allergens. 11.1.U8 Histamines cause allergic symptoms.

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11.1 Antibody Production and Vaccination

11.1.U10 Vaccines contain antigens that trigger immunity but do not cause the disease.

The by initiating a primary immune response, resulting in the production of memory cells that can produce antibodies in response to the antigen

A person is exposed to the vaccine orally or by injection

Vaccines contain antigens in various forms that should not cause symptoms in a healthy person.

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Mr Trent’s grandma comes into your medical practice and wants the latest flu vaccination. Can you help her?

11.1 Antibody Production and Vaccination

Oh Doctor Doctor

11.1.U10 Vaccines contain antigens that trigger immunity but do not cause the disease.

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11.1 Antibody Production and Vaccination

11.1.U10 Vaccines contain antigens that trigger immunity but do not cause the disease.

Vaccines contain antigens in various forms* that should not cause symptoms in a healthy person:

  • attenuated (weakened) or inactivated viruses
  • weakened toxins (produced by bacterial pathogens)
  • subunits – this could be the antigen or part of the pathogen carrying the antigen

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This was achieved by a worldwide vaccination programme. 1977 saw the last ever naturally occurring case of the disease in Somalia and in 1980 the World Health Organisation (WHO) declared the disease ‘dead’.

Eradication programmes for other diseases has reduced the number of cases, but has been less successful:

  • Polio and measles become contagious before symptoms are easy to detect –’Ring vaccination’ not possible
  • Immunity to Malaria is not complete and hence it can infect the same person a number of times
  • Yellow fever has an animal reservoir, it can also affect monkeys

11.1 Antibody Production and Vaccination

Smallpox (caused by the virus variola) was the first infectious disease of humans to have been eradicated by vaccination.

11.1.A1 Smallpox was the first infectious disease of humans to have been eradicated by vaccination.

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It was successful because of the following reasons -

  1. Patients were easily identified by obvious clinical features
  2. Transmission was through direct contact only
  3. There were no animal vectors or reservoirs where the disease could remain and re-emerge
  4. Contacts of the patients identified were quickly identified and vaccinated
  5. Immunity was long term so reinfection was unlikely
  6. The infection period was short-lived  (3 to 4 weeks)
  7. The virus was stable and didn’t mutate
  8. There was international cooperation organized by the WHO   �

11.1 Antibody Production and Vaccination

Smallpox (caused by the virus variola) was the first infectious disease of humans to have been eradicated by vaccination.

11.1.A1 Smallpox was the first infectious disease of humans to have been eradicated by vaccination.

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Modern medicine would consider Jenner’s testing procedure unethical:

  • no prior research had be done prior to human testing to determine the effectiveness and possible side-effects
  • informed consent was not given and the choice of a child who was to young to understand the dangers was even more questionable

Cowpox is a mild viral infection of cows, which is very similar to smallpox

In 1796 Edward Jenner deliberately infected an eight-year old boy with cowpox from pocks/blisters of a milkmaid with this disease.

Jenner repeated his initial on himself and a small group of people

Jenner then attempted to infect the boy with smallpox, but found that he was immune.

11.1 Antibody Production and Vaccination

Smallpox (caused by the virus variola) was the first infectious disease of humans to have been eradicated by vaccination.

Nature of science: Consider ethical implications of research - Jenner tested his vaccine for smallpox on a child. (4.5)

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11.1 Antibody Production and Vaccination

Why are tumour cells used in mass producing antibodies?

11.1.U11 Fusion of a tumour cell with an antibody-producing plasma cell creates a hybridoma cell.

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11.1 Antibody Production and Vaccination

11.1.A2 Monoclonal antibodies to HCG are used in pregnancy test kits.

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11.1 Antibody Production and Vaccination

Create a positive, negative and faulty pregnancy test and describe the science behind them.

11.1.A2 Monoclonal antibodies to HCG are used in pregnancy test kits.

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Pregnancy tests use monoclonal antibodies

Use the animations to find out how they work

11.1 Antibody Production and Vaccination

Create a positive, negative and faulty pregnancy test and describe the science behind them.

11.1.A2 Monoclonal antibodies to HCG are used in pregnancy test kits.

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11.1 Antibody Production and Vaccination

Epidemiology is the study of the incidence, distribution, and possible control of diseases

11.1.S1 Analysis of epidemiological data related to vaccination programmes.

Surveillance is critical to the control of Measles. Identifying and confirming suspected measles cases allows:

  1. early detection of outbreaks
  2. analysis of transmission helps to create more effective vaccination measures
  3. Estimation true measles incidence on reported data – reported incidence reflects a small proportion of the true number of incidences as many affected do not seek health care

Most Member States submit monthly reports on suspected and confirmed measles cases identified through their national disease surveillance systems to the WHO.

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11.1 Antibody Production and Vaccination

11.1.S1 Analysis of epidemiological data related to vaccination programmes.

Africa

Eastern Mediterranean

South-East Asia

Americas

Europe

Western Pacific

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11.1 Antibody Production and Vaccination

11.1.S1 Analysis of epidemiological data related to vaccination programmes.

Using the Measles case distribution by month. Analyse the date to answer the following questions:

  1. Outline the annual pattern in the data seen across all regions
  2. Identify the regions impacted mostly greatly by outbreaks.
  3. Since 2010 identify the regions in which the incidence of measles is:
    1. decreasing�
    2. Increasing�
    3. remaining constant�

  • Despite having an established vaccination programme in most countries Europe has seen a peak in Measles incidence between 2010 and 2013. Suggest a reason for this (hint: try an internet search on measles vaccination in Europe, in particular the UK).�

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11.1 Antibody Production and Vaccination

11.1.S1 Analysis of epidemiological data related to vaccination programmes.

Using the Measles case distribution by month. Analyse the date to answer the following questions:

  1. Outline the annual pattern in the data seen across all regions�Outbreaks start annually in January peaking in April/May then quickly declining in the summer months

2. Identify the regions impacted mostly greatly by outbreaks.�SE Asia, Western Pacific and Africa show similar levels on incidence to other regions outside of outbreaks, but dominate the case count during outbreaks.

3. Since 2010 identify the regions in which the incidence of measles is:

    • decreasing�Western Europe
    • Increasing�SE Asia, Western Pacific
    • remaining constant�Other regions show too greater variation to reliably judge an overall trend

4. Despite having an established vaccination programme in most countries Europe has seen a peak in Measles incidence between 2010 and 2013. Suggest a reason for this (hint: try an internet search on measles vaccination in Europe, in particular the UK).�The autism scare about the MMR vaccine wrongly lead people to choose not to have their children vaccinated – the evidence to support the scare was found to be false. This lead to outbreaks of Measles over a period of years when previously the disease had been well controlled.

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11.1 Antibody Production and Vaccination

Quiz

Plenary