1 of 19

Development of a Biopesticide Based Pesticide Residue Mitigation Strategy

Presented by Dennis Ndolo

Ag Aligned Global

2 of 19

(Part of) The Team

3 of 19

Residues

Early season pests

Late season pests

The last application is normally the driver of residues

4 of 19

Early season pests

Late season pests

Harvest

The strategy….

5 of 19

6 of 19

Residue mitigation studies

KENYA (KEPHIS)

Crop: Mango

Pest: Anthracnose

Test substance: Rodazim [carbendazim]

Biopesticide: Neem oil [Azadirachtin]

TANZANIA (TPHPA)

Crop: Avocado

Pest: Lepidopterans [FCM]

Test substance: Matron [methoxyfenozide]

Biopesticide: Cryptogran [Cryptophlebia leucotreta ]

7 of 19

Phase 1 (Residue decline)

Application* of conventional test substance according to label. �

Harvest crop & analyse to determine residue level.

* if a tank-mix of 2x test substances, then a lab analysis is conducted to determine which has the most persistent residue. This substance would then be used for Phase 2.

8 of 19

9 of 19

Phase 1 – Residue decline study

TEBUCONAZOLE RESIDUE DECLINE

CARBENDAZIM RESIDUE DECLINE

MRLs: no MRL in US (carbendazim is banned)

0.5 ppm in EU

5 ppm for Codex

MRLs: 0.15 ppm in US

0.05ppm Kenya and Codex

0.1 ppm in EU

10 of 19

Phase 2 - Introduction of Biopesticide

2 Replicate Fields – each consisting of 1 control plot (UTC) and 2 treated plots.

The first treated plot (TRT2) consisted of 2 conventional sprays of CARBENDAZIM (7 days apart) and one biopesticide spray of NEEM OIL which replaced the last conventional application. i.e LAST application replaced by a biopesticide application.

The second treated plot (TRT3) consisted of 3 conventional applications sprayed 7 days apart with the last application sprayed 14 days before normal harvest.

11 of 19

Test system design

12 of 19

Repeat Analysis – Same Trend

The results for the replicate 1 were as followed: lower residues in the biopesticide plot since the last conventional application was replaced by a biopesticide. The treated 02 residues were approx. 2X less that the residues for Treated 03 (all convent sprays). Replicate 2 –treated 02 samples resulted in average residues that were the same at day 0, day 21 and day 28. There was no decline in residue as seen at 21 day as in replicate 1. Treated 03 residues declined from 0.3 ppm to 0.14 ppm at day 21. It was requested for the samples to be rerun. These results showed a similar trend as the initial analysis. The SD and Dr.Braverman reviewed the field data and did not detect any issues with the data and laboratory data were reviewed by SD and Dr. Wayne who only detected one recovery slightly out of range at 69%. All other data looked good. Dr.Braverman also reviewed the analytical data stating MV and CR were fine; but the residue consistency across trials is not. Carbendazim is a persistent compound; therefore, it should have been the same persistence in all plots.

13 of 19

Pest Assessments – �Compare efficacy of biopesticide with conventional management�

Anthracnose

False Codling Moth

14 of 19

Biopesticides

Anthracnose = 70% Neem oil

Only biopesticide option

False codling moth = Cryptogran

Produced in South Africa
Compatible with natural enemies
Self perpetuating in field
No REI or PHI

15 of 19

Neem oil reduced leaf anthracnose

F = 0.08

P = 0.78

16 of 19

Neem oil reduces fruit anthracnose

F = 0.01

P = 0.94

17 of 19

No grower recommendations yet!

Anthracnose Management = Need additional replicates!

Variation in anthracnose infection levels
Different climates (rainfall)

False codling moth

Data coming soon