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Search and Research

Original draft:

Jordan Covvey, Mike Gionfriddo, Chyna McClendon,

Authors:

Karen Word, Hao Ye, & C4R CENTER Team

Community for Rigor

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What are the rigor problems? (for reviewers)

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  1. Researchers design or execute studies without adequate knowledge of prior work.
    1. What is known (orientation)
    2. What is not known (comprehensive search)
  2. Researchers do not know how to conduct comprehensive lit searches.
    • Skills & criteria for “comprehensive”
    • Risks of missing key papers

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What is this unit teaching? (for reviewers)

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  • Motivating core knowledge as key to rigor.
  • Setting goals for learning.
  • Introduction to platform heterogeneity.

Lesson 1

  • Learning efficiently with strategic reading
  • Setting reading goals
  • Planning strategies to achieve goals
  • Evaluating completeness
  • Consolidating learning

Lesson 2

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What is this unit teaching? (for reviewers)

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  • Conducting a targeted search, using PubMed.
  • Using boolean operators and field tags.

Lesson 3

  • Evaluating search results.
  • Targeted skimming of individual papers.
  • Iterating your search query and documenting changes.

Lesson 4

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What is this unit teaching? (for reviewers)

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  • Using an LLM to build a preliminary knowledge base of concepts, theories, methods, gaps, and references.

Lesson 5

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Unit overview

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  1. A new adventure
  • Exploratory learning
  • Targeted search
  • Iterative search
  • Learning with LLMs

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Orienting to a new adventure

Lesson 1

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To tell the story of search, we need to start at the beginning. Imagine we are embarking on research on a topic that is entirely new to us.

Lesson 1: Orienting to a new adventure

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Research projects start in many ways!

Familiar topic, new approach

Familiar approach, new topic

Broad interest in a topic

Ready-to-go project

A great idea!

Lesson 1: Orienting to a new adventure

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Imagine we are joining a project that already has a plan.

We’ll consider a simplified story.

Lesson 1: Orienting to a new adventure

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Study measurement: Word list task.

  • Day 1: study word list
  • Day 2: measure # words recalled

Hypothesis: Sleep deprivation interferes with memory.

Prediction: If we deprive subjects of sleep, their performance on a memory test will decline.

Before study: recruit subjects, collect demographic data

You’re hired! Here’s a project

Student volunteers

Total sleep deprivation (treatment).

Standard sleep (control).

Lesson 1: Orienting to a new adventure

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What are the risks of proceeding without detailed knowledge about sleep deprivation and memory?

Lesson 1: Orienting to a new adventure

There is never enough time to learn everything at the outset. The time to start work is usually ASAP.

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Halfway through, we chat with another scientist about our plan. They ask: “Why are you using a word recall test instead of [...]?”

Let’s say we get started.

We aren’t sure. Was there a better test to use?

Lesson 1: Orienting to a new adventure

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Now imagine: we take our completed research to a conference. Someone says: “Didn’t so-and-so publish something similar last year?”

Surely, someone would have checked for this?

Lesson 1: Orienting to a new adventure

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In this story, we might be unhappy with our collaborators. We trusted them to know! But here and always, we are responsible for the research we conduct.

Learning about a new topic can feel overwhelming. How can we apply rigor to learn what we need quickly and effectively?

Lesson 1: Orienting to a new adventure

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Learning everything is impossible, but with strategy, we can rapidly build a strong foundation.

We need:

Knowledge to explain key decisions about our project.

Confidence that we haven’t missed key publications that could affect our work.

Lesson 1: Orienting to a new adventure

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How would you approach learning about a new topic? Where would you begin, and when would you be ‘done’?

Think

Pair

Share

Discussion

Lesson 1: Orienting to a new adventure

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ACTIVITY: The right tool(s) for the job

First steps often involve a tool. How do you choose? Test your knowledge of the strengths and limitations of databases, search engines, and LLMs.

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Lesson 1: Orienting to a new adventure

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Increasingly, tools are combined to leverage diverse strengths. This can make it harder to know exactly what the limitations are. Still, knowing what questions to ask about a tool is a solid start to get the resources we need.

Now, let’s think about what we need to learn!

Lesson 1: Orienting to a new adventure

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Let’s consider that question we couldn’t answer about our choice of method. What would we need to know to explain that decision?

We want to know that the method is a practical and reliable way to produce evidence

We want to know that that type of evidence will usefully inform the theory under investigation

Lesson 1: Orienting to a new adventure

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A framework for scientific learning

How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

Lesson 1: Orienting to a new adventure

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We can’t evaluate a theory without evidence

How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

Lesson 1: Orienting to a new adventure

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How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

But without any theory, evidence is just information

Lesson 1: Orienting to a new adventure

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How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

We need evidence to know that a method works

Lesson 1: Orienting to a new adventure

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How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

But the meaning of evidence depends on the method used to generate it

Lesson 1: Orienting to a new adventure

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Let’s see how these categories can help to answer a question from earlier.

Lesson 1: Orienting to a new adventure

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“Why did you use that method?”

Method

What should our memory test tell us about brain function?

1

Evidence

  1. Has the memory test been validated?
  2. How is evidence from this test different from others in use?

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How will data from this test help support or rule out existing theories?

Theory

3

Lesson 1: Orienting to a new adventure

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Each piece is a manageable learning goal, or we can tackle them all together.

Lesson 1: Orienting to a new adventure

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In other words, we will have: Knowledge to explain key decisions about our project

When we can connect theory, method, and evidence for a component of a project, we can be confident that we understand its role.

Lesson 1: Orienting to a new adventure

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We still need: Confidence that we haven’t missed key publications that could affect our project.

Lesson 1: Orienting to a new adventure

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Knowledge to explain key decisions about our project and confidence we haven’t missed key publications are related.

Lesson 1: Orienting to a new adventure

Let’s explore how:

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Lesson 1: Orienting to a new adventure

Knowledge:

Explore, scope, and learn.

5. Specify method

Confidence:

Search for key details.

6. Set search goals

7. Literature search

1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

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Publications we don’t want to miss:

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Replication is great, but it should never happen by accident.

The same research

Has a metric been debunked as an indicator? An intervention deemed unrealistic?

Relevance implications

Could a different design have ruled that out?

Alternative hypotheses

A mediator that could have been measured.

Easy improvements

That technique actually detects D as well as A.

Method invalidations

Lesson 1: Orienting to a new adventure

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But… how?

We need a process!

Lesson 1: Orienting to a new adventure

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Where could we begin?

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Discuss it with an LLM.

Talk to someone who knows more.

Read a good review article or three.

Lesson 1: Orienting to a new adventure

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What do all of these resources have in common?

They give us things to read! What we do with those things makes all the difference.

Lesson 1: Orienting to a new adventure

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What we’ve learned, where we’re going

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  • Taking time to learn and find resources about a project is key to rigor.
  • With a little strategy, we can do this efficiently and effectively .

Lesson 1

Exploratory learning

Lesson 2

Targeted search

Lesson 3

Iterative search

Lesson 4

Learning with LLMs

Lesson 5

Lesson 1: Orienting to a new adventure

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What we’ve learned:

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Taking time to learn and find resources about a project is key to rigor.

With a little strategy, we can do this efficiently and effectively .

Lesson 1: Orienting to a new adventure

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Exploratory learning before a search

Lesson 2

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Before we continue: Why are you taking this lesson?

Make a note with 2-3 questions you think we might answer here.

Lesson 2: Exploratory learning before a search

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Science is a community effort.

Reading what people publish is only one way to learn from them.

What should we read? Ask for suggestions!

Have we understood correctly?

Ask for discussion! We’ll get help with this later in the lesson.

Lesson 2: Exploratory learning before a search

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In this lesson, we will discuss a process of reading to prepare for:

A discussion with an expert to confirm and extend our initial learning.

A targeted literature search.

Lesson 2: Exploratory learning before a search

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Started with a long list of papers and only had time to look at a few?

Read a paper carefully, only to find a more suitable paper later on?

Taken longer than you intended to read a paper?

Have you ever … ?

Wondered how (or if) anybody stays on top of their reading list?

Read and marked up a paper but still felt like you didn’t really understand it?

Assumed a paper was authoritative, only to have it called into question later?

Lesson 2: Exploratory learning before a search

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We are here to help!

These frustrating challenges are widely experienced and rarely discussed. We’re going to change that and share ways to tackle them!

Lesson 2: Exploratory learning before a search

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How do you read to learn about science? What strengths or tricks do you have? What is one thing you wish you could improve?

Think

Pair

Share

Discussion

Lesson 2: Exploratory learning before a search

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All researchers know how to read and learn.

But, we all read and learn differently! Some methods are more efficient for certain purposes than others.

Lesson 2: Exploratory learning before a search

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The reading process:

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1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

Recommendation

Casual search

Readings come from:

Lesson 2: Exploratory learning before a search

Where might we start?

A review article (best!).

Website, LLM output, etc (fine!).

The same strategies apply to all reading.

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Reading is necessary but not sufficient for learning.

Thinking is required!

Lesson 2: Exploratory learning before a search

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Goal-directed reading within the process:

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b. Plan a strategy

Identify goals in exploring a topic

a. Identify a resource

2. Read strategically for answers

1. Formulate questions

Lesson 2: Exploratory learning before a search

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Why are goals useful?

They make it easier to connect new information to our purpose and interests.

Goals aid learning!

We can focus on the most useful parts of the most useful resources.

Goals aid efficiency!

Lesson 2: Exploratory learning before a search

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Method

Sleep protocols, memory tasks, timing, population all vary between studies.

Evidence

Some findings are robust; others inconsistent or tentative.

Competing theories make different predictions.

Theory

Lesson 1: Orienting to a new adventure

What goals do we have for our study?

1. Formulate questions

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We might be new to our topic, but we still have prior knowledge.

A few possible questions might arise:

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Theory, Method, Evidence, Questions

What different methods have been applied?

What other theories have been suggested?

How much do we know about what is not yet known?

What do we know, or suspect, about theory, methods, and evidence?

Do we have a sense of what we might be missing?

How has prior evidence addressed questions?

What gaps remain that lack good evidence? Why?

Lesson 2: Exploratory learning before a search

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The solemn oath of strategic reading is: We will not read every word from start to finish without a specific reason for doing so.

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b. Plan a strategy

Everything else is open to negotiation!

Lesson 2: Exploratory learning before a search

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Depending on what we need from a text, we might:

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Read for data.

Skim for an overview.

Read for interpretation.

Read for details.

Read for gaps.

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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Skim for an overview

We survey all sections to see what they contain. We might learn things like:

The paper is organized around the timing of sleep deprivation relative to learning. Seems important!

The paper includes a meta-analysis, with some detailed figures that could be worth a closer look.

The authors discuss publication bias and have a long references list. Our first impression of credibility is good.

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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We scan the abstract, introduction, and conclusions, looking for text that reveals what the authors think it all means. We might find that:

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Read for interpretation

They think the strongest effects occur with one type of memory.

They note a widespread problem that weakens their confidence.

They believe recent findings are key, upending long held beliefs.

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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We examine figures and tables to see what kinds of data are presented. We might find that:

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Read for data

One table describes the many features of the included studies.

One figure aligns effects from all papers on a single plot to compare effect size and direction.

There are some figures that might take a little more work to understand, but we can at least identify their topic.

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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We text search for sections or references that answer a specific question we have in mind. We might text search for “recovery sleep” and learn:

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Read for details

Some researchers believe that testing after recovery from sleep deprivation can help separate memory impacts from other effects like attention.

Effect sizes are smaller in studies that allow recovery.

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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We scan the introduction & conclusion for words that signal still-missing knowledge (e.g., “unknown,” “future,” “yet”). We might learn that:

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Future studies are needed with larger sample sizes.

It is not yet known if memory consolidation may be spread across multiple nights of sleep.

The mechanisms connecting sleep deprivation to specific types of memory are still debated.

Read for gaps

b. Plan a strategy

Lesson 2: Exploratory learning before a search

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A note on reading for gaps:

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Lesson 2: Exploratory learning before a search

It can only find gaps that the authors are aware of and willing to discuss.

Text search has limits.

Unstated or unnoticed gaps within a paper may only become obvious with time.

More careful reading may be needed.

With experience and practice, we slowly learn to see what isn’t there.

Gaps are the essence of scientific progress!

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Speed reading strategies trade accuracy for speed.

Strategic reading involves saving time by being deliberate about finding and (accurately) extracting only what we need.

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Topic experts can often read quickly with accuracy. The rest of us need strategy.

Strategy ≠ Speed

Lesson 2: Exploratory learning before a search

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All strategies have a time and a place! The key is to be intentional. If our goal is to start and finish a learning task on a timeline, we should try a more disciplined strategy.

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Should we ever read a whole article, start to finish? What about letting our eyes wander in search of unknown surprises?

Lesson 2: Exploratory learning before a search

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Keshav (2007) suggests a more general method for reading for any academic literature.

A paper is read in three “passes.”

Progression to the next pass is optional, depending on purpose and what was learned in the last pass.

The 3 passes:

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A general approach: The Three Pass Method

3. Comprehensive (can take many hours).

1. Quick scan (~ 5-10 min).

2. Closer examination (~ 1 hour).

Lesson 2: Exploratory learning before a search

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Ready to try it out?

(optional: worksheet)

Lesson 2: Exploratory learning before a search

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Making reading worth the effort

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1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

However, evidence supports pausing to reflect, consolidate, and plan instead.

Strategic reading improves efficiency and learning.

It can feel compelling to just keep going!

Lesson 2: Exploratory learning before a search

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Evaluate:

Which questions have we found answers to?

What couldn’t we find in this resource?

Why might that be?

3. Evaluate learning

Lesson 2: Exploratory learning before a search

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Have we connected each piece?

Theory

Method

Evidence

Lesson 2: Exploratory learning before a search

3. Evaluate learning

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Consolidate:

What can we do to commit what we have learned to memory?

4. Consolidate learning

Lesson 2: Exploratory learning before a search

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What doesn’t work? (Dunlosky et al. 2013)

These are three examples of study strategies that are ubiquitous and feel effective but don’t hold up well in research on learning!

Copied down a summary (without comment)?

Re-read a book to learn its contents?

Used a highlighter or a pen to underscore important text?

Have you ever:

Lesson 2: Exploratory learning before a search

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What works? (Dunlosky et al. 2013)

e.g. reflecting before referring back

e.g. 1 hour per day for 5 days rather than 5 hours on 1 day

Strong support

Retrieval

Distributed learning

e.g. answering “why” questions

e.g. talking through an equation

Moderate support

Elaboration

Self-explanation

e.g. alternating topics

Interleaving

Lesson 2: Exploratory learning before a search

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Now what?

Independent learning is a little different from school. In school, an expert guides and assesses our learning. This is a huge help!

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1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

Lesson 2: Exploratory learning before a search

We can get the best results from our efforts by seeking out experts to guide and assess our learning

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Great conversations support learning!

Who can we reach out to?

Lesson 2: Exploratory learning before a search

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An “expert” might be:

A presenter we saw at a conference.

A recurring name in bibliographies.

A connection from a colleague.

Anyone with knowledge and experience that could help confirm and extend our learning!

Lesson 2: Exploratory learning before a search

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ACTIVITY: Reaching out for help

Writing a “cold” email to an author on a paper you’ve read is an intimidating task. How would you approach it?

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Lesson 2: Exploratory learning before a search

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The main goal? Send the email!

At the end of the day, your style is your own. Keep in mind that:

When you make mistakes, you are likely the only one who will remember them.

Most people find outreach emails intimidating.

Successful scientists know this is necessary and valuable.

Lesson 2: Exploratory learning before a search

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Preparing for a useful meeting

We’ll need to be prepared with questions!

Do we want them to teach?

They will need to understand the idea

It helps to specify the type of feedback

Do we want feedback?

We can prepare a prioritized agenda and keep an eye on the clock

Will time be limiting?

Preparation depends on the expectation:

Lesson 2: Exploratory learning before a search

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Preparing for a useful meeting

Remember:

Prepare to teach as well as listen.

Expertise has limits.

Before, during, and after a meeting are all good times to record ideas.

Notes, or it didn’t happen.

A quick email can cement the connection and lead to further conversations.

Follow up to affirm

Lesson 2: Exploratory learning before a search

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Return to the questions you wrote down at the beginning of this lesson! What have you learned? What questions remain?

Think

Pair

Share

Discussion

Lesson 2: Exploratory learning before a search

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Lesson 2: Exploratory learning before a search

5. Specify method

6. Set search goals

We covered:

Explore, scope, and learn

Next:

Search for key details

7. Literature search

1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

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Remember - we don’t want to miss:

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Replication is great, but it should never happen by accident.

The same research

Has a metric been debunked as an indicator? An intervention deemed unrealistic?

Relevance implications

Could a different design have ruled that out?

Alternative hypotheses

A mediator that could have been measured.

Easy improvements

That technique actually detects D as well as A.

Method invalidations

Lesson 1: Orienting to a new adventure

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Targeted (literature) searching

Lesson 3

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Performing a targeted literature search takes time and effort

When would you do this?

Lesson 3: Targeted (literature) searching

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Has anyone looked at this topic before? If they have, did they explore all the gaps and explanations?

You want to check that your focused research question is novel.

What are the standard methods for investigating this topic? What choices are involved and what are the justifications?

You want to select the best approach for your focused research question.

In both situations, you want a very specific set of results, but you ALSO want to be thorough…

Lesson 3: Targeted (literature) searching

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If you don't have a focused research question, wait!

This lesson is about conducting a comprehensive search in a narrow slice of the literature.

  • If you are learning about a topic, check out lesson 1.
  • If you are deciding on a research topic, look for our unit on Research Questions!

Lesson 3: Targeted (literature) searching

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We're going to use these skills:

Lesson 3: Targeted (literature) searching

1. Identify terms and synonyms—this helps us be thorough about our topic's key concepts.

2. Combine the terms and synonyms using boolean operators and field tags to create a focused search strategy.

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Suppose, after lessons 1-2, we chose this focused research question:

"How does acute sleep deprivation affect memory, and specifically verbal recall (in humans)?"

The key concepts here are:

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acute sleep deprivation

verbal recall memory

humans

Lesson 3: Targeted (literature) searching

Part 1. What are your terms and synonyms?

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acute sleep deprivation

verbal recall memory

humans

Intervention

Outcome

Population

What are the pieces of your study?

One way of breaking up a research question into key concepts is the PICO framework: population, intervention, control/comparison, outcome (Lasserson et al. 2024).

Lesson 3: Targeted (literature) searching

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Each key concept should be as specific as possible.

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humans

e.g.

Lesson 3: Targeted (literature) searching

What about humans with a particular condition?

Do we care about all humans or specific subgroups?

What about humans of a particular age?

Consider the following:

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Generate terms and synonyms

Some papers about a concept may use alternative phrasings.

We want to include all these papers, regardless of the specific phrasing.

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"verbal recall memory"

"verbal memory"

verbal recall memory

e.g.

Lesson 3: Targeted (literature) searching

"word learning"

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Combine synonyms using OR.

To include any paper using any of these different phrasings, apply this construction:

"verbal recall memory" OR "verbal memory" OR "word learning"

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verbal recall memory

e.g.

Lesson 3: Targeted (literature) searching

"verbal recall memory"

"verbal memory"

"word learning"

OR

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Antonyms can be useful!

In some cases, the opposite of your concept is a useful term to search for.

For example, for the concept

"verbal recall success",

papers that use

"verbal recall failure" or

"verbal recall deficit"

would be relevant too!

Lesson 3: Targeted (literature) searching

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In addition to different wordings of our concepts, we can also make use of database index terms.

Lesson 3: Targeted (literature) searching

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Many databases use a taxonomy of index terms to assign categorical labels to each paper (similar to hashtags).

PubMed's terms are called Medical Subject Headings (MeSH).

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verbal recall memory

Lesson 3: Targeted (literature) searching

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Many databases use a taxonomy of keywords to assign categorical labels to each paper (similar to hashtags).

In PubMed, the keywords are called Medical Subject Headings (MESH).

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verbal recall memory

Review the MeSH Tree

When viewing the entry for a MeSH term, be sure to review the tree structure to check if you want a related, more general, or more specific term.

Lesson 3: Targeted (literature) searching

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Many databases use a taxonomy of keywords to assign categorical labels to each paper (similar to hashtags).

In PubMed, the keywords are called Medical Subject Headings (MESH).

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Lesson 3: Targeted (literature) searching

verbal recall memory

https://meshb.nlm.nih.gov/record/ui?ui=D014706

Review the MeSH Tree

When you are viewing the entry for a MeSH term, be sure to review the tree structure to see related terms, including broader or more specific versions

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Apply proper search syntax for each term

In PubMed, format each term as "term"[tag], where:

  • term is the word or phrase, and;
  • tag is one of PubMed's field tags�(Here, we will use [tiab] for searching for a term in the title or abstract, and [mesh] for searching for a specific MeSH.).

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verbal recall memory

"verbal recall memory"[tiab] OR "verbal memory"[tiab] OR "word learning"[tiab] OR "verbal learning"[mesh]

Lesson 3: Targeted (literature) searching

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MeSH terms are exact!

MeSH terms must be the exact word or phrase that appears in the MeSH taxonomy.

"verbal learning"[mesh]

Variants or wildcards will NOT find results!

"verbal learn*"[mesh]

Lesson 3: Targeted (literature) searching

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After we have our lists of synonyms for each concept, what is next?

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"verbal recall memory"[tiab] OR "verbal memory"[tiab] �OR "word learning"[tiab] �OR "verbal learning"[mesh]

"acute sleep deprivation"[tiab] OR "sleep debt"[tiab] OR "inadequate sleep"[tiab] OR "insufficient sleep"[tiab] OR "sleep deprivation"[mesh]

Lesson 3: Targeted (literature) searching

verbal recall memory

acute sleep deprivation

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We want the papers in this overlap!

Combine the separate search queries with AND.

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Lesson 3: Targeted (literature) searching

"verbal recall memory"[tiab] OR "verbal memory"[tiab] �OR "word learning"[tiab] �OR "verbal learning"[mesh]

"acute sleep deprivation"[tiab] OR "sleep debt"[tiab] OR "inadequate sleep"[tiab] OR "insufficient sleep"[tiab] OR "sleep deprivation"[mesh]

verbal recall memory

acute sleep deprivation

AND

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("verbal recall memory"[tiab] OR "verbal memory"[tiab] OR "word learning"[tiab] OR "verbal learning"[mesh])

AND

("acute sleep deprivation"[tiab] OR "sleep debt"[tiab] OR "inadequate sleep"[tiab] OR "insufficient sleep"[tiab] OR "sleep deprivation"[mesh])

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Lesson 3: Targeted (literature) searching

"verbal recall memory"[tiab] OR "verbal memory"[tiab] �OR "word learning"[tiab] �OR "verbal learning"[mesh]

"acute sleep deprivation"[tiab] OR "sleep debt"[tiab] OR "inadequate sleep"[tiab] OR "insufficient sleep"[tiab] OR "sleep deprivation"[mesh]

verbal recall memory

acute sleep deprivation

AND

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Find terms for each concept:

Combine concepts together into a single search strategy:

That's a lot…let's do a little practice!

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Lesson 3: Targeted (literature) searching

Recap our steps

aggregate synonyms with OR.

find synonyms and alternate phrasings.

find matching index terms (if the database supports it).

apply quotes and field tags.

Make sure to add parentheses around the list of terms for each concept.

Combine concepts with AND.

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ACTIVITY: Practice building a search strategy

We've pre-generated a list of terms.

Identify and categorize them to build a search query.

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Lesson 3: Targeted (literature) searching

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Lesson 3: Targeted (literature) searching

You may need to adjust settings (from "Exact Match" to "Any Fragment") if your search phrase isn't part of an existing �MeSH term.

MeSH includes established concepts. It may not have new, unique, or niche terms or phrases.

Be sure to read the "Scope Note" to check that the definition matches.

Tips for the MESH browser

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MeSH terms can also have qualifiers that provide additional detail to the usage of a MeSH term.

Format as "verbal learning/classification"[mesh]

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verbal recall memory

https://meshb.nlm.nih.gov/record/ui?ui=D014706

Lesson 3: Targeted (literature) searching

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Tips for concept specificity

Lesson 3: Targeted (literature) searching

How flexible is your concept to your search needs?

Check if you want an abstract concept ("verbal memory") vs a specific measurement method ("paired association test")

Would any kind of memory work, or do you specifically need verbal recall?

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Lesson 3: Targeted (literature) searching

We focused on [tiab] and [mesh] for this lesson, but there are many more PubMed field tags available.

PubMed filters can help narrow search results by certain properties that don't easily translate into search terms:

Tips for other field tags and filters

type of paper (e.g. review, clinical trial, meta-analysis)

publication date (e.g. last 10 years).

species (e.g. mouse, human)

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Don't worry if your initial search isn't perfect—the next lesson is about improving your search.

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Lesson 3: Targeted (literature) searching

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Iterating a literature search

Lesson 4

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Your initial search isn't perfect, and that's OK.

Let's explore how to improve a search.

Lesson 4: Iterating a literature search

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Iterating on your search query

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1. Evaluate search results

2. Propose changes

3. Implement changes and document

4. Run the updated search

Lesson 4: Iterating a literature search

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("neuroplasticity"[tiab] OR "brain plasticity"[tiab] OR "Neuronal Plasticity"[MeSH] OR "neural plasticity"[tiab])

AND

("memory formation"[tiab] OR "Memory Consolidation" [MeSH] OR "memory encoding"[tiab])

How does neuroplasticity contribute to memory formation?

1869 results.1

That seems like a lot!

Lesson 4: Iterating a literature search

1PubMed search executed �7 May 2026.

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What are some reasons that a search can return unwanted results?

Think

Pair

Share

Discussion

Lesson 4: Iterating a literature search

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Results exclude important papers.

Results include irrelevant papers.

Our search query isn't lining up with our topic.

The topic is too broad. (too many results)

The topic is too narrow. (too few publications in this area)

Our topic isn't scoped well.

Lesson 4: Iterating a literature search

1869 results: why?

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Watch out!

Any individual issue can make the number of results too high or low.

That makes it impossible to diagnose issues just by checking the number of results.

We can only evaluate the effectiveness of our search by looking at the papers.

Let's take this one step at a time, and start with a single paper.

Lesson 4: Iterating a literature search

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It is well documented that sleep contributes to memory consolidation and it is also accepted that long-term synaptic plasticity plays a critical role in memory formation. The mechanisms of this sleep-dependent memory formation are unclear. Two main hypotheses are proposed. According to the first one, synapses are potentiated during wake; and during sleep they are scaled back to become available for the learning tasks in the next day. The other hypothesis is that sleep slow oscillations potentiate synapses that were depressed due to persistent activities during the previous day and that potentiation provides physiological basis for memory consolidation. The objective of this review is to group information on whether cortical synapses are up-scaled or down-scaled during sleep. We conclude that the majority of cortical synapses are up-regulated by sleep slow oscillation.

MESH: Humans, Memory / physiology, Neuronal Plasticity / physiology, Sleep / physiology, Synapses / metabolism

Igor Timofeev, Sylvain Chauvette

PMID: 28453998 DOI: 10.1016/j.conb.2017.03.019

Lesson 4: Iterating a literature search

Sleep slow oscillation and plasticity

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What is your first impression?

Relevant

Uncertain

Not Relevant

Scan for our concepts - does the paper focus on neuroplasticity and memory formation AND how they relate together?

Lesson 4: Iterating a literature search

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It is well documented that sleep contributes to memory consolidation and it is also accepted that long-term synaptic plasticity plays a critical role in memory formation. The mechanisms of this sleep-dependent memory formation are unclear. Two main hypotheses are proposed. According to the first one, synapses are potentiated during wake; and during sleep they are scaled back to become available for the learning tasks in the next day. The other hypothesis is that sleep slow oscillations potentiate synapses that were depressed due to persistent activities during the previous day and that potentiation provides physiological basis for memory consolidation. The objective of this review is to group information on whether cortical synapses are up-scaled or down-scaled during sleep. We conclude that the majority of cortical synapses are up-regulated by sleep slow oscillation.

MESH: Humans, Memory / physiology, Neuronal Plasticity / physiology, Sleep / physiology, Synapses / metabolism

Lesson 4: Iterating a literature search

Igor Timofeev, Sylvain Chauvette

PMID: 28453998 DOI: 10.1016/j.conb.2017.03.019

Sleep slow oscillation and plasticity

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It is well documented that sleep contributes to memory consolidation and it is also accepted that long-term synaptic plasticity plays a critical role in memory formation. The mechanisms of this sleep-dependent memory formation are unclear. Two main hypotheses are proposed. According to the first one, synapses are potentiated during wake; and during sleep they are scaled back to become available for the learning tasks in the next day. The other hypothesis is that sleep slow oscillations potentiate synapses that were depressed due to persistent activities during the previous day and that potentiation provides physiological basis for memory consolidation. The objective of this review is to group information on whether cortical synapses are up-scaled or down-scaled during sleep. We conclude that the majority of cortical synapses are up-regulated by sleep slow oscillation.

MESH: Humans, Memory / physiology, Neuronal Plasticity / physiology, Sleep / physiology, Synapses / metabolism

Lesson 4: Iterating a literature search

Igor Timofeev, Sylvain Chauvette

PMID: 28453998 DOI: 10.1016/j.conb.2017.03.019

Sleep slow oscillation and plasticity

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This result seems relevant. What's next?

Scan for additional terms and concepts. (e.g. processes, specific phenomena, hypotheses). These could indicate useful topics to research in more detail or ways to narrow our topic (if needed.)

Lesson 4: Iterating a literature search

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It is well documented that sleep contributes to memory consolidation and it is also accepted that long-term synaptic plasticity plays a critical role in memory formation. The mechanisms of this sleep-dependent memory formation are unclear. Two main hypotheses are proposed. According to the first one, synapses are potentiated during wake; and during sleep they are scaled back to become available for the learning tasks in the next day. The other hypothesis is that sleep slow oscillations potentiate synapses that were depressed due to persistent activities during the previous day and that potentiation provides physiological basis for memory consolidation. The objective of this review is to group information on whether cortical synapses are up-scaled or down-scaled during sleep. We conclude that the majority of cortical synapses are up-regulated by sleep slow oscillation.

MESH: Humans, Memory / physiology, Neuronal Plasticity / physiology, Sleep / physiology, Synapses / metabolism

Lesson 4: Iterating a literature search

Igor Timofeev, Sylvain Chauvette

PMID: 28453998 DOI: 10.1016/j.conb.2017.03.019

Sleep slow oscillation and plasticity

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It is well documented that sleep contributes to memory consolidation and it is also accepted that long-term synaptic plasticity plays a critical role in memory formation. The mechanisms of this sleep-dependent memory formation are unclear. Two main hypotheses are proposed. According to the first one, synapses are potentiated during wake; and during sleep they are scaled back to become available for the learning tasks in the next day. The other hypothesis is that sleep slow oscillations potentiate synapses that were depressed due to persistent activities during the previous day and that potentiation provides physiological basis for memory consolidation. The objective of this review is to group information on whether cortical synapses are up-scaled or down-scaled during sleep. We conclude that the majority of cortical synapses are up-regulated by sleep slow oscillation.

MESH: Humans, Memory / physiology, Neuronal Plasticity / physiology, Sleep / physiology, Synapses / metabolism

Lesson 4: Iterating a literature search

Igor Timofeev, Sylvain Chauvette

PMID: 28453998 DOI: 10.1016/j.conb.2017.03.019

Sleep slow oscillation and plasticity

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Aha, there are two competing hypotheses, and some additional terms for us to consider!

Lesson 4: Iterating a literature search

Sleep slow oscillations.

Synapse potentiation.

Regulation during sleep.

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Note any review papers!

As a review paper, this result will likely include references that are key studies or experiments on this topic.

Those papers might be useful for details on the design and execution of studies in this field.

Lesson 4: Iterating a literature search

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Possible actions from our evaluation:

(note: actions aren't mutually exclusive)

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Learn more about a specific term to obtain more background knowledge about the topic.

Narrow the focus of our search by including terms for one or more additional concepts.

Read through the paper in detail to obtain information (e.g. further references, methodological details).

Lesson 4: Iterating a literature search

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Let's look at a few more papers before taking any actions.

Lesson 4: Iterating a literature search

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ACTIVITY: Practice evaluating results

Examine three results from the same search. Then propose actions based on your evaluations.

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Lesson 4: Iterating a literature search

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Suppose you decide that there is a new concept that will help narrow your topic.

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Hippocampus CA3

"CA3 Region, Hippocampal"[mesh] OR "CA3 region"[tiab] OR "hippocampal CA3"[tiab]

Let's add:

Lesson 4: Iterating a literature search

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Search documentation is a habit that pays dividends, just like to maintaining a good lab notebook!

Be intentional with changes.

Conducting a search is like navigating a maze

Documentation helps you from going down the same dead ends

Lesson 4: Iterating a literature search

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Proper search documentation records:

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Paste your full search query and note any filters applied.

Query

Track time of search to establish a cadence for revisiting searches and remaining informed.

When

Record the database used and consider how you could port a query to another database.

Where

Explain why this is a distinct query and what you aimed for in assembling it.

Why

Document result count and any exemplar results to target for strategic reading.

Results

Lesson 1: Orienting to a new adventure

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A little goes a long way!

Documentation can start small. �The next time you search, open a spreadsheet and paste in the query! Let complexity evolve as you need it.

Lesson 2: Exploratory learning before a search

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We usually want to check that our search returns important papers.

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e.g. an exemplar paper that your PI or expert colleague gave you

Lesson 4: Iterating a literature search

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search query

date

# of results

Notes

ex. 1

("neuroplasticity"[tiab] OR "brain plasticity"[tiab] OR "Neuronal Plasticity"[MeSH] OR "neural plasticity"[tiab])

AND

("memory formation"[tiab] OR "Memory Consolidation" [MeSH] OR "memory encoding"[tiab])

2025-12-01

1831

initial search with concepts "neuroplasticity" and "memory formation"

Y

TBD

TBD

TBD

add "hippocampus CA 3" as a concept

TBD

Lesson 4: Iterating a literature search

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[[big long search query]] AND �"TITLE OF Important Example"

search:

Add a column to your search log to note the result.

Then proceed accordingly:

Yes

No

Great! Continue.

Hmm. Let's see what happened.

Check your results for a specific paper

Lesson 4: Iterating a literature search

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Does the example contain all of the concepts in your search?

Your search might be overly narrow.

Does the example use synonyms or alternate phrasings of the key concepts of your search?

You might need to update the synonyms for your concept(s).

Troubleshooting with an example

Lesson 4: Iterating a literature search

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Iterate your search as needed!

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1. Evaluate search results

2. Propose changes

3. Implement changes and document

4. Run the updated search

Lesson 4: Iterating a literature search

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An effective literature search is:

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Relevant—the search returns papers that match the desired topic.

Precise—the search does NOT include irrelevant results. (e.g. it is an effective filter)

Educational—Helps expand your knowledge of terms and concepts that are used in the literature.

Lesson 4: Iterating a literature search

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You may need a little help to dive into a search. Next we’ll discuss using an LLM for exploratory learning!

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Lesson 3: Targeted (literature) searching

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Exploratory learning (with LLM)

Lesson 5

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1. A conversation with a real person to confirm and extend essential knowledge

2. A comprehensive literature search to ensure that no key papers have been missed

If you don’t want to use LLMs, stay with us!

Many of the same strategies can be applied to monitor and improve independent learning.

In this lesson, we will apply an LLM to prepare for:

Lesson 5: Exploratory learning (with LLM)

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LLMs are useful tools for exploring ideas.

They are effective when we use them to make us think.

Lesson 5: Exploratory learning (with LLM)

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LLMs are not search engines or databases.

They are often confidently wrong.

Some citations and statements will be inaccurate or entirely fictional.

For more on this, look for our curriculum about LLMs!

Lesson 5: Exploratory learning (with LLM)

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Responsibility:

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1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

Lesson 5: Exploratory learning (with LLM)

Iterate quickly and endlessly

Customize to our exact level

Opportunity:

Validate everything

Explore ethics & options, e.g. when uploading content

Planning a process

with an LLM

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We can ask the model these questions, but like all content, responses will need validation.

Different models have different strengths:

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Choosing an LLM

Capability ≠ accuracy!

All models will make mistakes, even in their strong suits.

Can it access or review uploads to summarize?

How quickly can it work?

Can it access web search, or only predict links from training data?

Are inputs used for training?

What are its limitations?

Lesson 5: Exploratory learning (with LLM)

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Goals for optimizing

Resolving roadblocks

Are critical words or equations getting in the way?

Finding the right level

Do we want simpler language, or to see more jargon in use?

Playing with structure

How do we want to organize information?

Steps of a process

List of dominant theories

List of unanswered questions

Pros and cons of prior approaches

Lesson 5: Exploratory learning (with LLM)

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ACTIVITY: Playing with prompts

With an LLM, we can generate reading that meets us where we are. In this activity, you will start with your own prompt, then adjust and see the results!

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Lesson 5: Exploratory learning (with LLM)

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Lesson 5: Exploratory learning (with LLM)

More ideas for prompt enhancement

Describe audience, methods of interest, exemplar papers, target journals/conferences.

Share context about the project

State the goal and see how a suggested prompt compares to your original prompt.

Ask for feedback on the prompt itself

To reduce pandering, ask for help critiquing “a colleague’s” ideas.

Ask for critical evaluation

Being quizzed aids learning & reveals assumptions.

Ask for questions

Asking for evaluation of LLM response content in a new session or LLM can surface flaws or key details.

Reuse, recycle

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Lesson 5: Exploratory learning (with LLM)

Neuroscience

What else?

LLMs can zoom out in helpful ways

Psychology

Molecular biology

Where does our work fit? What distinguishes these fields?

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Stepwise exploration: sleep & memory

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  1. Each subject, broadly

“Give me an overview of research on sleep deprivation.” Then, “...memory.”

Starting broad offers context we might otherwise miss.

  1. Intersections

”... the impact of sleep deprivation on memory”

Does this seem almost as big as the broad topics? Maybe that says something.

  1. Narrowing

“Let’s focus on memory formation

Scoping down, we get specific about what really interests us.

Lesson 5: Exploratory learning (with LLM)

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Adjust goals to learn key details

How we think things work, and their alternatives.

Theory

Method

Available techniques and their challenges.

Evidence

Observations that inform theory or method.

Lesson 5: Exploratory learning (with LLM)

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Getting literature recommendations with LLM

Check the literature regularly and extend what we have learned.

Responsibility to validate

Explaining topics, suggesting references, and summarizing papers are very different tasks.

Right tool for the task

Papers sourced from LLMs should be 1) real and 2) good.

Are the papers useful?

Return to Lesson 2 for a focused, goal-directed reading session!

Why are we reading?

Lesson 5: Exploratory learning (with LLM)

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To use LLM to support our own reading, we can:

When using LLM to summarize papers, we should:

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LLMs and the reading process

Read the article itself for key content.

Know that uploaded content may be used to train the LLM.

Ask the LLM to direct us to key content within the article.

Leverage capacity for synthesis e.g. compare papers, highlight differences.

Type in something we’ve learned for feedback.

E.g “Evaluate the accuracy of this statement: the difference between short and long term memory is…”

Ask for help with unclear statements.

E.g. “What does this paper mean when it says “...””

Lesson 5: Exploratory learning (with LLM)

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The research process with LLMs

1. Formulate questions

2. Read strategically for answers

3. Evaluate learning

4. Consolidate learning

Lesson 5: Exploratory learning (with LLM)

Using both, carefully, is a powerful way to learn & focus our plans.

LLMs explain well but are often wrong.

Review articles are accurate but harder to read.

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A cautious process with LLM can be very productive. However, it still can’t replace a great conversation with a real person!

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Revisit lesson 2 for help!

A word on reaching out to real people

Building community

Accuracy

Knowing what you actually need to know

Humans are still best at:

Lesson 5: Exploratory learning (with LLM)

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Lesson 5: Exploratory learning (with LLM)

1. Ask questions

2. Clarify answers

3. Seek reference recommendations

4. Read & synthesize

5. Specify method

6. Set search goals

Explore and scope a topic

Search for key details

7. Literature search

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Lesson 5: Exploratory learning (with LLM)

Let’s review:

A structured and thoughtful engagement with established work solves headaches and heartaches.

Lesson 1:

Reading should be approached strategically and in conversation with experts.

Lesson 2:

Databases speak specific languages, and we can use those languages to search properly.

Lesson 3:

An effective search is relevant, precise, and educational.

Lesson 4:

Thoughtful (and careful!) interaction with LLMs �can support learning.

Lesson 5:

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No literature search is perfect; using the right tools and techniques can help us learn what matters!

Lesson 5: Exploratory learning (with LLM)

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Better Science Every Day

Search and Research

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