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Validation

Of Bowie Dick Test & Biological Indicator Test

Wan Maiza Binti Wan Zahari

Penolong Pengarah Kanan Kejururawatan

Bahagian Kejururawatan KKM

(Merangkap Ketua Perkhidmatan Pensterilan Berpusat (KKM)

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Introduction of Bowie & Dick Test

  • Developed by Dr. J. Bowie and

Mr. J. Dick in 1963.

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  • Evolution and upgrade to “16 towel chalange”.

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  • To check that the autoclave sterilizer is functioning properly, the Bowie & Dick Test are carried out before the autoclave sterilizer is used.

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How Bowie & Dick Test Function

  • Ensure that air is properly removed from the sterilisation chamber
  • And loads are fully penetrated with steam

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Steam Penetration

Air Removal

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Composition of the Bowie-Dick test pack

2017 Association for the Advancement of Medical Instrumentation ■ ANSI/AAMI ST79:2017

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Composition & Challage

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Pass

Fail

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Pass – Sterilizer is functioning in good conditions

Pass – Sterilizer is showing sign of failure. Sterilizer servicing is required.

Fail – Sterilizer must be shutdown, pending repair and may need re-qualification if repair is deemed significant.

Overexposure – gray or silvery appearance, overexposure (time and/or temperature) is indicated; a retest is required since the test is invalid.

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Why is it important?�

  • To confirm the effectiveness of the initial vacuum phase and, therefore, how well steam penetrates cavities.
  • To detact : -
  • Clogged drain lines, ventilation line or water filter.
  • Leaks caused by incorrectly sealed doors or other defects.
  • Low steam pressure.
  • Water temperature too high.
  • Poor quality of steam.
  • Variations in steam supply.
  • Steam condensation.
  • Non-condensable gases.

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BI

Attest

Spore test

Biological Indicator

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Conventional BI

Rapid BI

48 hours

24 hours

“Hantar Lab”

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Conventional BI

48 hours

24 hours

“Hantar Lab”

&

Ampoule Media

Spore Disc

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Geo Bacillus Stearo Thermophilus

Spore

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CONTROL TUBE

INCUBATE 60°C

24 Hours

POSITIVE RESULT

PROCESSED TUBE

INCUBATE 60°C

24 Hours

NEGATIVE RESULT

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TERRJAH

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Rapid BI

3 hours

1 hour

30 Min

20 Min

2 hours

30 Min

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Media

INCUBATE 60°C

RESULT READING & VALIDATION

BY INCUBATOR

FLUORESCENT READING

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TERRJAH

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Thank you