FLIPPED CLASSROOM: HOW DO BACTERIA TALK?

1. Why are scientists studying how bacteria communicate?
2. What is quorum sensing?
3. Describe how Vibrio fischeri manipulate quorum sensing in the Hawaiian Bob Squid.
4. According to Prof. Bassler, what is the near-future application to combat antibiotic resistance bacteria?
5. Name the lab equipment shown in the video

Nutrient Limitation

Why???

Diversity/ Survival

Damage Repair

GENETIC ENGINEERING

(BSM 3202)

WEEK II

Learning Outcomes

To be able to describe the natural occurrence of DNA transformation (C2) and the human applications of the process (C4)

Frederick Griffith, 1928.

Natural transformation by Streptococcus pneumoniae

Frederick Griffith, 1928.

Natural transformation by Streptococcus pneumoniae

IN 1944, AVERY, MCCARTY AND MACLEOD PROVED THAT DNA WAS THE TRANSFORMING PRINCIPLE.

What is the way that bacteria transfer/acquire foreign DNA naturally?

“SEX” in bacteria = lateral gene transfer

THINGS TO-DO

Horizontal Gene Transfer (HGT)

Working in SCL GROUP as indicated below:

Natural transformation for Gram +

GROUP 1,2,3

Natural transformation for Gram –

GROUP 4,5,6

Conjugation

GROUP 7,8

Transduction

GROUP 9,10

Review the infographic given in the Trello and summarise them!

• description of the mechanism as a whole
• components involved to operate those transfer
• a selected bacterium as example to model your transfer mechanism.
• General comparison between the three (3) transfer mechanism.

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• REFERENCES (can be QR code)

SUBMISSION THROUGH PUTRA BLAST

HTTP://LEARNINGHUB.UPM.EDU.MY/BLASTDK/

• INCLUDE LIST OF MEMBERS AS THE FILE COVER PAGE.
• SAVE FILE AS YOUR GROUP NAME (Eg. Group A)
• ACCEPTABLE FILE FORMAT = PDF ONLY
• SELECTION OF BACTERIA HAVE TO BE UNIQUE TO YOUR GROUP
• MARKS WILL BE AUTOMATICALLY DIVIDED FOR GROUP WITH THE SAME BACTERIA

SUBMISSION THROUGH PUTRA BLAST

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DATELINE: 1^st Nov, 9 am

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Failure to submit and not to follow requirements before will deemed your submission unrecorded and unmarked.