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Overcoming the Challenges of Organoid Research and Development

#90-002850424

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Leveraging Organoids to Advance Human Health

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Modeling of tissue growth and homeostasis
Understand host-microbe interactions
Drug discovery and biobanking
Recapitulate disease pathology

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Common Organoid Origins and Applications

iPSCs

Primary tissue
iPSCs
Cryopreserved lines

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Enabling Efficient Organoid Processing and Maintenance

Processing Challenges

LevitasBio Solutions

Removal of unhealthy cells
Unwanted cell types that contaminate culture
Dissociation requirements for sorting of cells
Efficient regeneration of organoids post-processing

LeviCell® Systems

Removal of unhealthy cells
Unwanted cell types that contaminate culture
Dissociation requirements for sorting of cells
Efficient regeneration of organoids post-processing

Enriches for healthy, viable cells and removes debris
Gentle processing for sensitive organoids
No dissociation required
Facilitates organoid generation after processing

Each of these processes have their own challenges such as the efficient and effective removal of unhealthy and/or unwanted cells that can contaminate the culture, the need to dissociate organoids for sorting and enrichment steps, and the efficient regeneration of healthy organoids post-processing….and this is where we come into play. Levitation Technology enables researchers to enrich for healthy, viable cells, which are free of major debris and dead cell contaminants. As you know, the process is very gentle on the cells, and due to the large size of the cartridge, no dissociation of the organoids of interest is required when enriching for cells of interest (though the technology is compatible with both whole and dissociated organoids). Aside from facilitating the processing of organoids, levitated samples improve scientist’s ability to regenerate cultures for future downstream readouts and subsequent storage and use.

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Elegant Simplicity

Live Whole Organoids

Dead Cells/Debris

Split Line

Viable cells levitate above the split line
Debris and dead cells fall below the split line
Simple, simultaneous, and semi-automated enrichment
Wide separation channel handling up to 350 μm
Gentle flow <1 psi

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Levitating Healthy Organoids

Dead Organoids

Live Organoids

Human Gastric Organoids

Gentle process reduces processing stress
No dissociation required for enrichment
Simple and rapid process
No need for antibodies or dyes

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Remove Dead and Dying Organoids

Colorectal Cancer Organoids

Enrich for viable organoids from any origin
Remove dead/dying cells
Simultaneous debris removal
Suitable for both whole and dissociated organoids

n=3

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Visualizing Organoid Health in Real-Time

Calcein

PI

Human Gastric Organoids

Calcein

PI

Visualize during sample preparation
Fluorescent dyes are optional
Generate additional QC parameters with LeviMetrics^TM and instrument software

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Enhanced Organoid Generation

	No levitation	Levitation
Particles per Dome	1,040	3,388
Mean Diameter (all)	23.7	24.0
Estimated output (vials)	0.9	2.9

No levitation

levitation

Day 0

Day 5

Day 5 Assessment

Improve downstream culture efficiency
Reduce time and required resources
Maximize production number and quality

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Maximize Sample Preparation with Levitation Technology

Simplified, combinatorial workflow
Fast, gentle & semi-automated process
Provides exceptional enrichment for a variety of organoid types
Removes debris & increases viability
Maximize organoid potential