1 of 40

�LOT RELEASE

What is Lot Release?

1

2 of 40

�LOT RELEASE

What is Lot Release?

The process of reviewing each individual batch of a licensed product by the National Regulatory Authority (NRA) before giving approval for its release to the market.

Lot Release is applied to biological products including vaccines.

2

3 of 40

LOT RELEASE

Why do we need lot release for vaccines?

  • Vaccines are biological products used in the large number of healthy individuals. The impact of using sub-standard lot will have a direct impact on public acceptance of immunization programmes and potentially compromising public health strategies.
  • In Vaccine the tests applied to them are of a biological and complex in nature and have an potential for variability . Therefore an independent review of manufacturing and QC data of each lot of vaccine is essential to assure the consistent quality of each manufactured lot.

3

4 of 40

LOT RELEASE

  • Finally, reference standards used in the testing of vaccines are also biological and complex in nature as the vaccines themselves. For new products, National or International standards or reference preparations are not always available and there may be limited data on the stability of in-house or working standards used. Independent review of data is necessary in order to gain confidence in results of tests using these preparations.

4

5 of 40

SUMMARY LOT PROTOCOL

  • A Summary Lot Protocol’ is a document summarizing all manufacturing steps and test results for each lot of vaccine
  • It is prepared by the manufacturer for each lot of vaccine
  • It is abbreviated to SLP

5

6 of 40

SUMMARY LOT PROTOCOL

Principle

An independent review of critical data from SLP of each lot of vaccine is essential in order to :

  • assure the consistency of quality of each manufactured lot;
  • obtain confidence in the claimed strength of active components;
  • assess the validity and accuracy of the tests performed

6

7 of 40

SUMMARY LOT PROTOCOL

  • Based on the WHO model the manufacturer can design the SLP as per the information required for the production of vaccine and the tests performed at each steps.
  • WHO provides a model of a summary lot protocol for most vaccines in the TRS. for e.g, DTP, Measles, Oral Polio, Rabies, BCG and Hepatitis B Vaccines.
  • Some countries have additional requirements for vaccines additional tests or information they design their own format.

7

8 of 40

SUMMARY LOT PROTOCOL

  • Since protocol review is an essential component of the lot release process, it is crucial that the template of the summary protocol is developed carefully on the basis of the approved marketing authorization dossier which is approved by the NRA/NCL.

8

9 of 40

SUMMARY LOT PROTOCOL

  • The template should be a controlled document and the manufacturer should not change it without the approval of the regulatory authority. It is important that NRA/NCL staff responsible for reviewing these documents ensure that the latest version of the license and post approval changes is reflected in the summary protocol submitted by the manufacturer.

9

10 of 40

SUMMARY LOT PROTOCOL

  • Name of the Product: Final products, final bulk and the diluents if applicable.
  • Name of Manufacturer: For Traceability.
  • Lot Number : For Traceability.
  • License Number : Unique license number.
  • Site of Manufacturing for each bulk, final bulk and final product.

10

11 of 40

SUMMARY LOT PROTOCOL

Manufacturing Details

Strains

Name of the Strain /Seed used in production, Passage number and type of cell substrate,

Master or working lot/ bank number

All the information's should be same as the one approved by the NRA on the marketing authorization.

11

12 of 40

SUMMARY LOT PROTOCOL

Manufacturing Details

  • The manufacturing process for major components from starting materials, intermediates, final bulk and final product confirmed.
  • The methods of quality-control tests as well as their release specifications and the results obtained; are within the acceptable range.

12

13 of 40

SUMMARY LOT PROTOCOL

Formulation

  • Amount of active components in the final formulations, with the lot numbers and volumes of bulk concentrates; storage conditions Verify calculated and actual values based on information provided.

Final Product

  • Lot size Volume, number of doses and type of container Listed information should fit within allowed parameters
  • Expiry dates For each starting material (if applicable), intermediates, final bulk and final product Expiry date of each component fits the shelf-life of the final product

13

14 of 40

SUMMARY LOT PROTOCOL

  • Quality-control tests
  • Information of reference preparations, standards and internal controls.
  • Actual results of tests on critical starting materials, intermediates, final bulk and final product along with the approved specifications of individual tests.
  • if applicable; include results of failed tests or note invalid tests if a test has been repeated

14

15 of 40

WHO Global Benchmarking Tool (GBT)�for LOT RELEASE

WHO and a team of regulators prepared a list of 6 critical indicators and 17 sub-indicators for Evaluation of National Regulatory System of Lot release of Vaccine.

15

16 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

16

No.

LR01.01

Sub indicator

Legal provisions and regulations exist to conduct and enforce lot release for all vaccines.

Maturity level

1

Description

Remarks

The assessor should verify the existence of legal provisions, regulations that give the National Regulatory (NRA) authority to implement and enforce lot release for vaccines. The legal provisions should define the responsible officer authorized to sign the regulatory lot release certificate.

The Central Drugs Laboratory (CDL) has been designated as the National control laboratory to take responsibility for Quality control testing of vaccine as per the provision of Chapter –II, section 6 of Drug & Cosmetic act, 1940 The function of the Central Drugs laboratory is prescribed in Rule-3-A of part-II of Drugs & Cosmetic Rules, 1945 and the laboratory has the mandate for testing Vaccines & sera .Toxoid and Antitoxins. The central government has appointed Director and government analysists to sign the lot release certificate and legal samples reports

17 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

17

No.

LR01.01

Sub indicator

Legal provisions and regulations exist to conduct and enforce lot release for all vaccines.

Maturity level

1

Description

Remarks

The assessor should verify that the legal basis to perform lot release applies for all vaccines marketed in the country which imported and domestically- produced vaccines.

The lab has been given the mandate for lot release by letter no. X-11026/4/95-D, X-11026/9/2001D and as per the instructions of DCG (I) and ministry vide letter No: X-11026/11/2017-BD that each lot of indigenous produced vaccine and imported is to released by CDL, kasauli before its marketed. (UNICEF, PAHO, Export, EP{, Domestic trade and state government supplies )

Current approaches for conducting lot release of vaccines include :

  1. Review of the summary protocol only
  2. Review of the summary protocol with independent testing (i.e., either full or selected testing)

18 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

18

No.

LR01.01

Description

CDL has the policy to release vaccine on the basis of Review of the summary protocol with independent testing (either full or partial testing).

The assessor should verify that the criteria have been defined when the NRA may elect to follow non-routine procedures for lot release for example, a shortage of a product on the market or the need to import non- authorized product for a defined time.

No Exemption from Lot Release.

19 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

19

No.

LR01.01

Sub indicator

Legal provisions and regulations exist to conduct and enforce lot release for all vaccines.

Maturity level

1

GAP: As per the orders of Ministry of Health & Family Welfare, Nirman Bhawan, New Delhi dated 2012 That Central Drugs Laboratory (CDL) Kasauli is delinked from Central Research Institute Kasauli and is under the full and direct control of Drug Controller General of (India) in Central Drugs Standard Control organization(CDSCO)/

Amendment in Drug and Cosmetic act required to change Director Central Drugs Laboratory instead of Director Central Research Institute Kasauli.

20 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

20

No.

LR01.02

Sub indicator

Acceptance policy and criteria for lot release performed by another NRA are documented.

Maturity level

2

Description

If the country does not have the capacity to perform lot release on its own, the assessor should determine whether the country recognizes certificates from other competent NRAs and NCLs. These processes include creation of mutual agreements

21 of 40

Indicator: LR01 Legal provisions, regulations and guidelines required to define regulatory framework of independent lot release by the NRA.

21

No.

LR01.02

Sub indicator

Acceptance policy and criteria for lot release performed by another NRA are documented.

Maturity level

2

Remarks

CDL requires the Lot release certificate from country of origin However its not the sole criteria for lot release of the imported vaccine. No communication is received till date from NRA that MOU has been signed between NRA and International Regulatory Agencies to Acceptance of lot release certificates from the responsible NRA or National Control Laboratory (NCL).

GAP : MOU has to be signed between NRA and International Regulatory Agencies to Acceptance of lot release certificates from the responsible NRA or National Control Laboratory (NCL). avoid Redundant testing.

22 of 40

Indicator: LR02 Arrangement for effective organization and good governance.

22

No.

LR 02: 01

Sub indicator

There is a defined organizational structure with clear responsibilities to conduct independent lot release activities.

Maturity level

2

Description

The assessor should verify that roles and responsibilities for all regulatory entities involved in the independent lot release are documented and implemented. It is critical that the roles and responsibilities of both the NRA and the NCL are clearly defined, particularly when they are separate entities.

Legal Mandate :The Central Drugs Laboratory (CDL) has been designated as the National control laboratory to take responsibility for Quality control testing of vaccine as per the provision of Chapter –II, section 6 of Drug & Cosmetic act, 1940 The function of the Central Drugs laboratory is prescribed in Rule-3-A of part-II of Drugs & Cosmetic Rules, 1945 and the laboratory has the mandate for testing Sera, Vaccines, Toxoid and Antitoxins. Director and Government analysist are authorised to sign the Lot release certificate.

23 of 40

Indicator: LR02 Arrangement for effective organization and good governance.

23

No.

LR 02: 01

Sub indicator

There is a defined organizational structure with clear responsibilities to conduct independent lot release activities.

Remarks

Source of Samples :

  1. Indigenous vaccine Manufacturer (b) Different Port offices when vaccines are imported (c) Survey samples from Manufactures premises as seized / confiscated by Drug Inspectors (d) Samples drawn & seized by Drug Inspectors from field.

After receiving the samples a unique sample receipt number is assigned to each sample . The testing is completed within the time period assigned by the authorities and the batch release certificate is released and a copy of the same is marked to NRA for information. Job Responsibilities of each personnel are defined in the Quality Manual

24 of 40

Indicator: LR02 Arrangement for effective organization and good governance.

24

No.

LR 02: 02

Sub indicator

Documented procedures are implemented to ensure coordination and communication among all regulatory entities involved in independent lot release.

Maturity level

3

Remarks

Communication between NCL and NRA is very good.

Any communication to the Manufactures is marked to DCG(I) Office for information.

NRA sends CMC modules and Post approval changes to review to NCL Kasauli. After taking opinion from NCL kasauli NOC is released from NRA and a copy is send to NCL Kasauli.

25 of 40

Indicator: LR03 Human resources to perform NRA lot release.

25

No.

LR 03: 01

Sub indicator

Sufficient competent staff (i.e., education, training, skills and experience) are assigned to perform NRA lot release activities.

Maturity level

3

Description

Remarks

The assessor should verify that the human resources assigned to perform NRA lot release activities should be sufficient with respect to numbers and competent with respect to the requisite skills, education, experience and training.

The Laboratory has academically qualified staff. Testing personnel have experience in biochemical and microbiological testing techniques. Staff is also trained in Quality Management Systems for laboratories such as IS/ISO17025 :2017. For efficient performance of lot release function additional qualified and experienced staff on contractual basis is appointed other then regular staff.

26 of 40

Indicator: LR03 Human resources to perform NRA lot release.

26

No.

LR 03: 02

Sub indicator

Duties, functions, and responsibilities of the staff in charge of NRA lot release activities are established and updated in the respective job descriptions.

Maturity level

3

Description

Remarks

The guidance document should present the appropriate duties and responsibilities that are assigned to each member of the organization involved in NRA lot release activities. Thus, the professional profiles of staff are reflected in their respective roles and responsibilities within the NRA.

The organization chart of the Laboratory is well document

Job descriptions of each staff is part of QM.

27 of 40

Indicator: LR03 Human resources to perform NRA lot release.

27

No.

LR 03: 03

Sub indicator

Training plan developed, implemented and updated at least once a year for staff in charge of NRA lot release activities. Training plan developed, implemented and updated at least once a year for staff in charge of NRA lot release activities.

Maturity level

3

Description

Remarks

The assessor should verify that training plans are developed, implemented and updated at least once every year to reflect the current situation by considering education and experience of the staff.

Staff Training Plan is in place the IDP is monitored every year

  1. Induction training is given to newly appointed staff.
  2. Need based training is given to on job staff for the newer methods to be implemented .
  3. Staff is also trained in Quality Management Systems for laboratories such as IS/ISO17025 :2017.

28 of 40

Indicator: LR03 Human resources to perform NRA lot release.

28

No.

LR 03: 04

Sub indicator

The NRA generates and maintains records of staff training activities and training effectiveness verification.

Maturity level

3

Description

Remarks

The assessor should verify that records of staff training that is performed or organized by the NRA are generated, maintained, regularly updated.

CDL maintain the training records along with the Sample evaluation questionnaire.

29 of 40

Indicator: LR04 Procedures established and implemented to perform NRA lot release.

29

No.

LR 04: 01

Sub indicator

Independent lot release is based, at a minimum, on summary lot protocol review and the appropriate documentation exists.

Maturity level

2

Description

Remarks

The assessor should verify that summary lot protocols are mandatorily required for the lot release of vaccines. The assessor should make sure the NRA or NCL issue lot release certificates based, at minimum, on the review of the summary lot protocols issued by the manufacturer. The protocol review should be performed by comparing the critical data of each lot (including testing data) to the licensed product specifications.

Lot release certificate is issued by CDL on the minimum review of summary Lot protocol issued by manufacturer. The protocol review is performed by comparing critical data of test results of each lot.

30 of 40

Indicator: LR04 Procedures established and implemented to perform NRA lot release.

30

No.

LR 04: 02

Sub indicator

NRA or NCL staff involved in lot release have access to MA relevant files and updates.

Maturity level

3

Description

The assessor should verify that the NRA or NCL staff involved in lot release has access to files and updates relevant to MA.NRA or NCL lot release should be performed only for medical products that have a valid MA in which specifications have been approved by the competent NRA or NCL of the country using the vaccine. In addition, the development and adoption of more effective test methods should be encouraged; however, any changes in testing should be approved by the NRA or NCL. If a different test method is used by the NRA or NCL, and if there is a discrepancy in test data between the manufacturer and the NRA or NCL, then the approved test method defined in the MA should be used to resolve the issue.

31 of 40

Indicator: LR04 Procedures established and implemented to perform NRA lot release.

31

No.

LR 04: 02

Sub indicator

NRA or NCL staff involved in lot release have access to MA relevant files and updates.

Maturity level

3

Remarks

CDL reviews CMC Module-III Of CTD submitted to NRA. However any Post Approval Change if any is again reviewed by CDL and Opinion submitted to The National Regulatory Authority and recorded If a different test method is used by CDL and if there is a discrepancy in test data between the manufacturer and CDL the NRA or NCL, then the approved test method defined in the MA is used to resolve the issue.

32 of 40

Indicator: LR04 Procedures established and implemented to perform NRA lot release.

32

No.

LR 04: 03

Sub indicator

Analysis of lot-to-lot consistency is conducted..

Maturity level

3

Description

Remarks

The assessor should verify that statistical analyses are conducted once sufficient data have been accumulated.

CDL makes the trends on yearly basis by comparing the results of CDL with the manufacturer results When CDL does not receive consecutive lots, or when it receives only a small number of production lots, interpretation of trends may require additional information (e.g., yearly biological product reports).

33 of 40

Indicator:LR05 Mechanism for information-sharing exists to promote transparency and accountability

33

No.

LR 05: 01

Sub indicator

Results of lot release process are publicly available.

Maturity level

4

Description

Remarks

The assessor should verify the existence of a list of product batches that pass or fail NRA Lot release. In addition, the assessor should verify that the NRA or NCL has the required authority and an available process to publish the list of product batches that passed or failed NRA lot release. At a minimum, a publicly available website The assessor should determine the frequency at which this information is updated

CDL has its own website https://cdlkasauli.gov.in.

All the batch release information is available on the website and the frequency for uploading the information is fortnight.

34 of 40

Indicator:LR05 Mechanism for information-sharing exists to promote transparency and accountability

34

No.

LR 05: 02

Sub indicator

Follow-up and communication with involved parties, including the manufacturer, on issues of data quality.

Maturity level

3

Description

Remarks

The assessor should verify the establishment and implementation of communication procedures with involved parties, including the manufacturer, on issues of data quality. Good communication with the manufacturer of the product is an important element in developing an effective system.

The procedure are available to communicate QC and national lot release issues which include letter, email and physical discussion CDL has a got the SOP for Transfer of methodology from manufacturer’s.

Depending upon the nature and severity of the discrepancies or errors, the manufacturer may be asked to perform an investigation to determine the root cause of the issues, and to initiate any corrective and preventive actions required to avoid similar problems A feedback mechanism is also available at CDL

35 of 40

Indicator:LR06 Mechanism in place to monitor regulatory performance and output.

35

No.

LR 06: 01

Sub indicator

Lot release records, reports and certificates available.

Maturity level

3

Description

Remarks

The assessor should verify that there is requirement to issue a certificate of release for all vaccines that have undergone lot release. This release certificate is issued by the responsible NRA or NCL on the basis of, at a minimum, a review of the lot summary protocol for the relevant lot. The assessor should verify that lot-to-lot consistency is appropriately analysed and documented by the NRA or NCL on regular basis.

CDL has got the checklist for all the products to review the summary lot protocol. CDL has the policy to release vaccine on the basis of minimum Review of the summary protocol with independent testing (either full or partial testing). There is no exemption from Lot Release. Records of trends analysis are available to verify that lot-to-lot consistency.

36 of 40

Indicator:LR06 Mechanism in place to monitor regulatory performance and output.

36

No.

LR 06: 02

Sub indicator

Corrective actions taken in case of deviations due to laboratory error

Maturity level

3

Description

Remarks

The assessor should review that appropriate corrective actions are taken by the NCL in case of deviations (i.e. significant discrepancy between NCL testing results and manufacturer results due to laboratory or operator error and not due to product quality).

SOP for CAPA is available in CDL Kasauli.

37 of 40

Indicator:LR06 Mechanism in place to monitor regulatory performance and output.

37

No.

LR 06: 03

Sub indicator

Regulatory action taken in case of product non-compliance.

Maturity level

3

Description

Remarks

The assessor should verify that in case of non-compliances, the NRA or NCL should confirm, through appropriate laboratory investigation, that the non-compliant results reflect the quality of the lot tested and is not due either to an analytical error by the NCL

Decision making in case of out of Specifications

If the test batch submitted to CDL is found OOS the test is repeated as per the retest policy. If the test results are not confirming the specifications ask the manufacturer to discuss the results. If the manufacturer is satisfied with the results declare the sample as NSQ and ask the manufacturer to find the root cause. If the manufacturer is not satisfied with the results and want to retest the batch perform

38 of 40

Indicator:LR06 Mechanism in place to monitor regulatory performance and output.

38

No.

LR 06: 03

Sub indicator

Regulatory action taken in case of product non-compliance.

Maturity level

3

Remarks

the test in duplicate in the presence of the representative of manufacturer . If both the samples meet the specifications Declare the batch as standard quality. If only one sample meets the specifications declare the sample as NSQ and ask the manufacturer to investigate the root cause and communicate to CDL Kasauli, Its communicated to NRA for further necessary action.

39 of 40

Indicator:LR06 Mechanism in place to monitor regulatory performance and output.

39

No.

LR 06: 04

Sub indicator

Performance indicators for national lot release activities are established and implemented.

Maturity level

4

Description

Remarks

The assessor should verify the existence and implementation of performance indicators for different activities included under the national lot release functions. Specifically, the system should define key performance indicators (KPIs) along the entire national lot release activity chain

CDL Kasauli is sending the samples details on 5th of every month to CDSCO headquarters in the KPI percribed format. CDL follows SOP “Time frame for assessment of samples for Quality”.

For protocol scrutiny the product is released within 21 days for vaccines having single antigen and 28 days for vaccines having multiple antigens

All the products for partial testing are released in 30 days

The products which are subjected to complete testing for in vitro testing are released in 30 days and for in-vivo testing in 90 days subject to after receiving all the necessary documents.

40 of 40

40

THANKS