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Higher Environmental Science @ Gairloch High School

Name:

Lesson 1 - Measuring abiotic factors in a terrestrial ecosystem

Lesson 2 - Measuring abiotic factors in an aquatic ecosystem

Lesson 3 - Identifying and monitoring pollution incidents

Lesson 4 - Environmental assessment

Sustainable Development Goals

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Environmental monitoring describes the processes and activities that need to take place in order to characterise and monitor the quality of an environment over time. In this topic you will learn about how to monitor the environment using different tools and methods:

  • The use of biotic indices (to monitor populations e.g. capture-mark-recapture) and biodiversity indices (e.g. Simpson’s diversity index)
  • The monitoring of abiotic & edaphic factors

The purpose of environmental monitoring is to recognise any impacts on the environment, respond to them and provide data for future legislation and guidance.

Abiotic factors are those that relate to a �non-living feature of an ecosystem. In �this lesson, we will learn how abiotic �factors are measured in a terrestrial �ecosystem. A terrestrial ecosystem is �one based on land.

Measuring temperature

Temperature is measured using a �thermometer. The units are usually oC

  • Air temperature is measured using a standard wall thermometer, which �must be mounted or used out of direct sunlight
  • Soil temperature is measuring using a probe thermometer
  • Surface temperatures are measured using an infrared thermometer

Measuring light intensity

This is measured using a light meter. It is measured in lux. It’s important to be consistent in how you use a light meter, for example always holding it at the soil’s surface and making sure the position of your body isn’t impacting the result. Light intensity can impact on the growth of different plants, which can in turn impact a whole food chain.

Measuring soil moisture

This can be done in two ways, depending on the location:

  • In the field, a soil moisture meter can be used to give a % reading. This measures the �electrical conductivity of the soil and converts it into an estimated percentage. This way of �measuring soil moisture is quick but rarely accurate, so it’s important to take several �measurements and calculate an average. The probe must be cleaned after each use.
  • In the lab, soil moisture can be calculated using weight. First, the weight of the soil sample is taken. The soil is then heated to a point where the water evaporates from the soil sample. It is then weighed again, and the percentage of soil moisture calculated.�

Measuring soil pH and nitrate/nutrient levels

Both of these properties are studied using soil testing kits. Collect a sample of soil and �follow the instructions on the testing kit to prepare the sample. Colour is used to �compare the pH or nitrate content of the soil to a standardised chart. Nitrate is an �important nutrient for plant growth. Although some plants can tolerate nitrate-poor �soils, many will need nitrogen to grow.

Soil pH and nitrate can vary widely in a small area, due to the influence of vegetation �or other soil characteristics. Several samples should be taken and an average calculated.

Date: Lesson 1 - Measuring abiotic factors in a terrestrial ecosystem

Higher Environmental Science @ Gairloch High School

% soil moisture =

Wet weight (g) - dry weight (g)

Wet weight (g)

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Lesson 1 - Measuring abiotic factors in a terrestrial ecosystem

Measuring wind velocity and direction

Wind velocity is measured using an anemometer and the speed is recorded in knots or metres �per second. Wind direction is measured using a wind vane. The narrow end of the wind vane �points in the direction the wind is coming from. Wind velocity can impact the spread of seeds and the height of plants which can grow.

Measuring precipitation

Precipitation is moisture that falls from the air to the ground. It includes rain, sleet, snow, �hail, drizzle, fog and mist. Precipitation is usually measured with a rain gauge. These can be �both traditional (read by a human) or electronic (storing and sending data digitally). They �should be placed and used in an area which is not under a canopy of vegetation - unless �that is what you are trying to study!

Measuring slope

Measuring the angle of a slope is done using a clinometer. This displays�the angle between two equivalent points, usually using a ranging pole.�The diagram shows how this is completed.

Most slopes vary, with some sections being steeper than others. If this�is the case, multiple measurements are taken and a slope profile is �constructed.

A typical slope profile of a psammosere is shown on page 12 of your Biodiversity booklet.

Task 1 - Investigation

You will work in groups to investigate the relationship between a biotic factor and abiotic factor in a sand dune system.

e.g. how does wind speed affect species richness through a psammosere

To do this you should use a series of three transects and random systematic sampling.

You will present the results of your investigation as a written report. The template for this will be given to you.

Deadline for completion:

Higher Environmental Science @ Gairloch High School

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Lesson 1 - Measuring abiotic factors in a terrestrial ecosystem

Task 2

Complete the table which summarises abiotic factors in a terrestrial ecosystem.

Higher Environmental Science @ Gairloch High School

Abiotic factor

Equipment used

Units of measurement

How to measure… [give some detail]

Temperature - air

Temperature - soil

Temperature - surface

Light intensity

Soil moisture

Soil pH & nutrients (e.g. nitrates)

Wind direction

Wind speed

Precipitation

Slope

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Date: Lesson 2 - Measuring abiotic factors in an aquatic ecosystem

Higher Environmental Science @ Gairloch High School

Abiotic factors are those that relate to a non-living feature of an �ecosystem. In this lesson, we will learn how abiotic factors are measured in �an aquatic ecosystem. Aquatic ecosystems include oceans, lakes, rivers, streams, �estuaries and wetlands.

Measuring temperature

Temperature is measured using a thermometer. The units are usually oC. The �temperature of water can vary with depth, so it should be measured at the surface �or a consistent depth. It should also be measured in a shaded or unshaded location. �Sea temperatures are measured by data loggers positioned at moorings or buoys �(e.g. in Loch Ewe).

How have humans been changing water temperature?

Measuring water flow rate

This is generally used when studying rivers and streams and is sometimes called the �river flow or discharge. There are mobile options which have an impeller on the end �of a stick and in some places these are permanently installed (e.g. River Ewe and �River Kerry).

The flow of the water turns the impeller. The meter then calculates the water �flow rate (usually in m3/s) .

Deforestation

  • Decreased shading
  • Increased runoff

Water abstraction

  • Drinking water storage

Anthropogenic climate change

  • Altered weather
  • Altered hydrological cycle

Urban

  • Increased surface run-off
  • Drainage inputs
  • Urban heat island
  • Shading from buildings

Industrial cooling

  • River water extraction
  • Cooling water return

Agriculture

  • Irrigation water extraction
  • Irrigation water return
  • Groundwater extraction

Dams

  • Water released from bottom
  • Water released from top

River change & diversion

  • Channel straightening
  • Increased width/depth ratio

Sewage

  • Wastewater return

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Measuring dissolved oxygen content

Dissolved oxygen concentration is a measure of the amount of free oxygen dissolved in water, which can be used to determine the quality of water and assess its ability to support life.

Water bodies produce and consume oxygen. They gain oxygen from the atmosphere and from plants, as a result of photosynthesis. Running water, because it is turbulent, dissolves more oxygen than still water. Respiration by aquatic animals, decomposition and chemical reactions all consume oxygen.

  • Wastewater from sewage treatment plants often has a low oxygen content, as organic materials are decomposed by microorganisms. This consumes oxygen.
  • Runoff from farmland has a higher than usual nitrogen content, due to the use of fertilisers. This causes more algae to live, increasing the amount of oxygen consumed during respiration.

Dissolved oxygen levels change based on the seasons and even the time of day. This is because photosynthesis, which produces oxygen, cannot happen in the absence of light (e.g. at night). They are also affected by temperature and altitude (height above or below sea level). Cold water holds more oxygen than warm water and at high altitude, water holds less oxygen.

Samples of water tested for dissolved oxygen must be collected carefully, as leaving an air gap in the bottle would cause more atmospheric oxygen to get into the sample. A bottle with a small neck is used and held at 45o under the water until it’s full. The lid is then put on under the water, before the sample is �lifted.

Then, the sample is tested using reagents. These react with the oxygen and �give a colour change. The level of dissolved oxygen can then be interpreted. �Alternatively, a dissolved oxygen sensor and a data logger can be used if �available.

Measuring biological oxygen demand

Biological Oxygen Demand (BOD) is a measure of the amount of dissolved oxygen used by aerobic microorganisms when decomposing organic matter in water. It is measured in mg/l (milligrams per litre) of oxygen consumed over a 5 day period at 20oC. The level of dissolved oxygen in the sample is compare before and after the sampling period. In a high BOD sample, the amount of dissolved oxygen will be much less after 5 days.

This can have an impact on aquatic life, as the high demand for oxygen will then result in there not being enough oxygen available to support a wide range of species. High BOD environments can cause mass deaths of aquatic life.

Measuring pH

The pH of water can be tested using litmus paper or universal indicator. In general, pH in Scottish rivers is lower than pH 6, although at times it will approach neutral. Underlying chalk or limestone rocks can give a more alkaline result but these are very rare in Scotland.

Measuring salinity

A hydrometer is used as a simple measure of salinity. It measures the �specific gravity, or density, of liquids. It consists of a glass tube with a bulb �weighted at one end, and calibrated markings along the length of the tube.�It floats at different levels based on the density of the liquid. Salinity is�measured in parts per thousand (ppt) or %.

Lesson 2 - Measuring abiotic factors in an aquatic ecosystem

Higher Environmental Science @ Gairloch High School

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Task 1

Use the Fisheries Management Scotland webpage to answer the following questions:

  1. What is the ideal temperature range for Atlantic Salmon in Scottish rivers? ………………………………………
  2. At what temperature do Atlantic salmon demonstrate thermal stress? …………………………………………
  3. How regularly is thermal stress recorded in Scottish rivers? …………………………………………………………�………………………………………………………………………………………………………………………………
  4. Referring to the diagram shown on page 5 and using the rest of the information on the webpage, tick all the human activities which are mentioned to be impacting Atlantic salmon by causing a change in water temperature.
  5. Describe and explain one intervention that’s being made to reduce thermal stress in Scottish rivers. ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Task 2

Complete the table summarising how to measure abiotic factors in an aquatic ecosystem.

Lesson 2 - Measuring abiotic factors in an aquatic ecosystem

Higher Environmental Science @ Gairloch High School

Abiotic factor

Equipment used

Units of measurement

How to measure… [give some detail]

Temperature - water

Water flow rate

Dissolved oxygen content

Biological oxygen demand (BOD)

pH

Salinity

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Task 3

Using the equipment provided in class, create a hydrometer and work out the relative salinities of the different samples of water. Relative means in relation to each other.

Task 4

  1. What are two ways that water bodies gain oxygen? …………………………………………………………………�………………………………………………………………………………………………………………………………
  2. What are three ways that water bodies consume oxygen? …………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  3. Would you expect a river in its upper course or lower course to have a higher dissolved oxygen content? Explain your answer. ………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………
  4. The results of three water samples are given below.
    1. What is the mean water temperature? ………………………………………………………………………
    2. What is the range of altitude? …………………………………………………………………………………
    3. Predict the values of dissolved oxygen content in the samples A and B, based on the data about the water temperature and altitude.

������

    • Which sample location would you expect to support the widest range of aquatic life? Explain your answer. …………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
    • Location C is in an urban area. What are three ways that the dissolved oxygen content of this part of the river could be improved? ……………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Task 5

Create a scientific poster outlining how abiotic factors affect the frequency and distribution of organisms. A template will be provided and you should include three factors from terrestrial ecosystems and three factors from aquatic ecosystems.

Lesson 2 - Measuring abiotic factors in an aquatic ecosystem

Higher Environmental Science @ Gairloch High School

Sample location

Water temperature (oC)

Altitude (m above sea level)

Dissolved oxygen content (mg/l)

A

14.0

6.5

B

17.8

16

7.5

C

22.5

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Sources of pollution

Point source pollution is discharged from a single location, usually from a pipe, chimney, or other outlet. If the point source can be found, the problem can be tackled relatively easily.

Most point source discharges of pollution are small and aren’t a large problem. However, they can combine to form diffuse pollution which can have a significant environmental impact.

Diffuse pollution arises from land activities spread across large areas that have no specific point of discharge. This includes runoff from farmland, forestry activities, urban areas, roads and industrial premises.

Monitoring freshwater pollution

Pollution of a river or loch can be identified by:

  • A change in colour, usually due to the�presence of different chemicals
  • Dead animals (e.g. floating fish)
  • Presence of debris (e.g. wipes)
  • Regular monitoring
  • Algal blooms

Pollution of rivers can be studied in a �laboratory, where the presence of different �chemicals and pollutants in water can be �found and measured.

It can also be studied in the field, using �aquatic invertebrates in the river to tell us �about how clean or polluted the waterway is.

How can we study the biota of a river?

Some aquatic invertebrates are only able to survive �in high quality, unpolluted water. By counting the number �of these species in different locations or at different times �we can study how polluted, or unpolluted, a river is.

Kick sampling is a method used to obtain a sample�of aquatic invertebrates. Begin by agitating the stream �bed with a boot (or use a stick or tool) upstream

from a flat edged net held against the stream bottom. �Using a quadrat to mark an area, ‘kick’ for two minutes, covering the whole area evenly. This will dislodge �organisms and wash them into the flow of the stream and into the net.

Place the catch in a shallow white tray with 2 cm depth of fresh water from the stream or river. Then, use an identification key to identify groups of species.

Date: Lesson 3 - Identifying and monitoring pollution incidents

Higher Environmental Science @ Gairloch High School

Polmadie Burn, Glasgow polluted by Chromium from a disused chemical works.

Types of pollution

Water pollution

Litter

Noise pollution

Plastic pollution

Soil contamination

Radioactive contamination

Thermal pollution

Light pollution

Visual pollution

Noise pollution

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Date: Lesson 3 - Identifying and monitoring pollution incidents

A biotic index is a scale showing the quality of an environment based on the types of organisms which inhabit it. So far we have learned about two biotic indices:

  • Simpson’s Diversity index - a measure of biodiversity
  • Lincoln index - an estimate of total population

We can also use biotic indices to study the impact of humans on ecosystems.

The Trent biotic index uses the presence or absence of different species to study the pollution of freshwater and efforts to improve water quality. It was developed by the Trent river authority to detect water pollution.

A score of 0 represents highly polluted water and >10 represents clean water. Scores are determined by the presence of different indicator species. Many of these “clean water” indicator species need a high amount of dissolved oxygen in the waterbody.

Many freshwater invertebrates look similar and have subtle differences in their morphology (shape) and size which can make identifying them tricky. A paired statement key can be used for this.��Paired statement keys rely on visible differences between �species to identify them. This means that if species have been�crossbred or hybritised (e.g. some varieties of vegetable, to�improve yield) they become difficult to identify using this �technique.

Higher Environmental Science @ Gairloch High School

Worms, including flatworms, leeches and midge larvae are indicator species for polluted water.

Mayfly and stonefly nymphs are examples of indicator species for clean, unpolluted water.

In practice, there are many types of biotic index used to study freshwater environments such as rivers and ponds. The Trent biotic index is a complicated one and, although you need to know it’s name and the basics of how it works, we will use a simpler one when studying freshwater pollution in the field.

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Lesson 3 - Identifying and monitoring pollution incidents

Task 1

Complete this table to describe the different types of pollution.

Task 2

Explain why each of these instructions is important when undertaking kick sampling:

  1. Using a flat edged net ……………………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  2. Standing upstream of the net ……………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  3. Timing or counting kicks …………………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  4. Using a quadrat ……………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  5. Using a white tray …………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Higher Environmental Science @ Gairloch High School

Type of pollution

Point or diffuse pollution?

Tourists choosing to leave rubbish beside a full waste bin at Red Point beach car park.

A sewer at Thurso High School has become blocked and has overflowed into the adjacent River Thurso.

The Polmadie Burn has turned yellow. SEPA have identified an old chemical works as the source of the pollution.

Slurry, applied to a field during a period of high rainfall, has run into an adjacent soft fruit field.

Environmental monitoring of Melvich beach has uncovered a radioactive particle.

Acoustic fencing is installed along the edge of a new housing development which backs onto the A9.

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Lesson 3 - Identifying and monitoring pollution incidents

Task 3

  1. Using the leaf features table, �construct a paired statement key �which will allow the identification of �silver birch, oak, ash and sycamore �trees. You should plan this on scrap �plan this on scrap paper and copy �the final key in below.���������������������
  2. Use the paired statements in the FSC Tree Name Trail to identify species of tree from around the school.��������

Task 4

Capture-mark-recapture and kick sampling are both methods of sampling and studying a population. These are both invasive techniques.

Non-invasive techniques include studying footprints, droppings and using trail cameras. What are three advantages of using non-invasive methods to study populations?�

…………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………

Higher Environmental Science @ Gairloch High School

Species recorded:

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Lesson 3 - Identifying and monitoring pollution incidents

Higher Environmental Science @ Gairloch High School

Key Environmental Agency: Scottish Environmental Protection Agency (SEPA)

SEPA is one of Scotland’s key environmental agencies. It’s role�is to protect Scotland’s air, land and water, specifically:

  • discharges of wastes to water, beaches and bathing �waters,
  • emissions to the air
  • abstraction (taking out of) of water from surface water and groundwater sites
  • waste sites (e.g. landfill, recycling centres, waste contractors, movement of waste, illegal waste disposal)
  • diffuse pollution
  • noise at industrial sites
  • use, storage & disposal of radioactive material or waste
  • flood warnings

SEPA has around 1,300 staff and its headquarters are in Stirling. It has offices and laboratories across the country including in Dingwall. They also have a number of roles which are suitable for working from home.

Along with the other key environmental agencies, SEPA enforces legislation related to Scotland’s environment, advises Scottish ministers, local authority planners, land owners, land managers, land users and voluntary organisations. They also help shape national policies (a plan of action that focuses on a specific target), educate the public and conduct research and monitoring. They do a lot of partnership working with other agencies, organisations and charities (e.g. Scottish Water).

Task 5

  1. What does SEPA stand for? …………………………………………………………………………………………
  2. SEPA are responsible for many different types of pollution. What are four sources of pollution? ………………………………………………………………………………………………………………………………
  3. Using the SEPA Jobs site, complete this table.

  • Look at the current SEPA vacancies and the range of salaries on offer for different roles.

Role

Job title

What does this job do?

What do you need to do this job?

Environmental Monitoring

Assistant Scientist

Environmental Protection

Environmental Protection Officer

Supporting Roles

Health & Safety

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Lesson 3 - Identifying and monitoring pollution incidents

Higher Environmental Science @ Gairloch High School

Task 6

The following is from the October 2023 Wester Ross Fisheries Trust (WRFT) newsletter.

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Lesson 3 - Identifying and monitoring pollution incidents

Higher Environmental Science @ Gairloch High School

Use the newsletter article to answer the following questions:

  1. Why is the Kinlochewe river important for ecology? …………………………………………………………………�………………………………………………………………………………………………………………………………
  2. What evidence was there in the field of pollution? ……………………………………………………………………�………………………………………………………………………………………………………………………………
  3. Is this an example of point or diffuse pollution? Explain your answer. …………………………………………�………………………………………………………………………………………………………………………………
  4. What three other factors were increasing the impact of the pollution? …………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  5. Research: What is a fatberg is and how can it cause pollution of rivers? …………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  6. Research: What is a minnow and and how does it react to pollution incidents? …………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  7. The Buglife Riverfly Monitoring protocol is a way of studying the biota of rivers to identify pollution. Watch the video about how this is done.
    1. How many indicator species are there? [These indicate clean water] ……………………………………
    2. What are three examples of indicator species? ………………………………………………………………�………………………………………………………………………………………………………………………
  8. Both SEPA and WRFT recorded unexpected results.
    • What was the result? ……………………………………………………………………………………………
    • Explain what the result tells us about the Kinlochewe river. ………………………………………………�………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
  9. SEPA is one of Scotland’s key environmental agencies. Other agencies include NatureScot, Forestry and Land Scotland (FLS) and Scottish Forestry (SF), and Marine Scotland (MS). What role did SEPA play in the pollution incident? ………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………
  10. Scottish Water is not an environmental agency, but was still important in the pollution incident. What role did they play? ………………………………………………………………………………………………………………�………………………………………………………………………………………………………………………………

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People make decisions on a daily basis in order to match their priorities. We’ve �seen so far that humans have been making decisions, over many years, which prioritise �economic and social development over protecting our environment, biodiversity �and climate.

Increasingly, people are choosing or being required to take the environment�into account when making decisions. In this lesson we’ll learn about some of the�formal ways to do this.

Environmental assessment is the process of estimating and evaluating �significant short-term and long-term effects of a programme or project on �the quality of the location’s environment.

There are three formal types of environmental assessment which must be made in Scotland:

  • Environmental Impact Assessment (EIA) for evaluating the major environmental effects likely to happen as a result of a proposed development project.
  • Strategic Environmental Assessment (SEA) which has to form part of all public plans, programmes and strategies.
  • Habitats Regulations Appraisal (HRA) for plans that are likely to have an impact on specially protected sites. Knowledge of the role of HRA is not part of the Higher Environmental Science course.

Environmental Impact Assessment (EIA)

An EIA aims to protect the environment by ensuring that a local planning authority (e.g. Highland Council) has full knowledge of possible significant environmental effects of a proposed development, and mitigation of these, which are taken into account in the decision-making process.

The assessment:

  • is carried out by – or on behalf of – the developer or project proposer
  • covers all stages of the project – from site surveys and construction to operation, monitoring and decommissioning
  • is written as a report which is submitted with the planning application

Not every project needs an EIA - only those which will have a major impact on the environment. A process called screening is used to decide whether an EIA is needed. Then, the EIA process begins with scoping in which the project developer considers what aspects of the environment might be affected by the proposal. During this stage they will engage with organisations who might be impacted by the project, or who could provide advice. They are known as consultees. The report is then considered by planners when deciding whether the development should be given planning permission.

The redevelopment of Uig Harbour in Skye was a project which would have a major impact on the environment. An EIA was carried out by AECOM on behalf of the Highland Council.

Lesson 4 - Environmental Assessment

Higher Environmental Science @ Gairloch High School

The different environmental effects considered. For each, �they outlined the existing environment (e.g. the water quality was measured), how the development could �affect this and what mitigation measures �could reduce the impact.

A long list of stakeholders were identified during the EIA process

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Strategic Environmental Assessment (SEA)

SEA aims to provide a high level of protection of the environment from development. It is mandatory for plans and/or programmes that relate to large-scale changes in land use. It differs from EIA as it focuses on plans, rather than developments. SEA is primarily undertaken by public bodies and utility companies.

For example, in 2024 the Scottish Government were coming up with plans for changes to how and when wild deer can be culled. They had to undertake an SEA to ensure that they have considered the environmental effects of any new legislation.

Government policy

A policy is a plan of action that focuses on a specific target. The methods and principles needed to achieve the policy are set out in a strategy.

Legislation (laws) and initiatives (actions) are then implemented in order to achieve the aims of the strategy and policy.

Lesson 4 - Environmental Assessment

Higher Environmental Science @ Gairloch High School

A scoring system can give a clear overview of effects on the environment

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Task 1

The information on the following pages comes from the 2023 Environmental Science Question Paper 1.

Paper 1 tests your knowledge of environmental science by asking you to apply it to a real life (case study) situation - in this case oil field decommissioning. You might not know much at all about oil field decommissioning, but you know enough about environmental science to use the resources provided to answer the questions.

The case study is introduced

Resources are given to support your answers

Lesson 4 - Environmental Assessment

Higher Environmental Science @ Gairloch High School

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Guided Revision

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Guided Revision

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