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CII initiative – Davey lab

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What we like to do

Filo

Arena

CCHFV

Drug identification

Host target identification

Gysi et al. PNAS 2021

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rEBOV dual tagged with NP-FLAG HA-VP35

Hela cells, 100-fold dilution, 24 hpi Confocal, 60X

FLAG

Nuclei

HA

Overlay

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Seeing the virus program with the microscope

2 hours

20+ hours

4 hours

Cell entry

Early replication

Late replication

NP

nucleus

vRNA

Callie Donahue

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Two parameter VP35 localization reveals gene function

  • Distinguish host factors involved in genome replication from cell entry
  • Used parameters of punctate vs faint VP35 staining

Entry blockers

Replication

blockers

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Summary

  • Timing of virus infection is key for disease
  • The cell is highly evolved to detect infection
  • Viruses are highly evolved to avoid detection
  • Balance between detection avoidance and cell manipulation is finely tuned
  • Exploiting this dependence is effective in treatment development

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