1 of 32

Spectroscopic Mapping & Monitoring �Coastal Wastewater Pollution �in the Tijuana River Estuary �and San Diego Coastal Ocean

Dan Sousa

+ a big team at SDSU and beyond

San Diego State University

14 May 2025

2 of 32

Overview

  1. Problem Background

  • Approach

  • Results

2

3 of 32

Problem Background

  • San Diego / Tijuana beaches are impacted by untreated wastewater
  • 5 million inhabitants, 35 million annual visitors (San Diego Chamber of Commerce, 2021)
  • Substantial impact to local economy (tourism, small business traffic) and public health (contaminants, disease)
  • Also a global issue (>1.8 billion people worldwide, largely in low-resource countries, impacted by untreated wastewater & associated diseases (Ryder, 2017)

3

4 of 32

Wastewater Discharge in the Tijuana River

  • One major source of this pollution in the San Diego / Tijuana metro is the Tijuana River

  • Billions of liters of wastewater are expelled annually – directly into the river channel

  • Carries harmful pollutants through two major cities (> 3 million residents) and a protected estuary.

4

5 of 32

Wastewater Discharge in the Tijuana River

  • One major source of this pollution in the San Diego / Tijuana metro is the Tijuana River

  • Billions of liters of wastewater are expelled annually – directly into the river channel

  • Carries harmful pollutants through two major cities (> 3 million residents) and a protected estuary.

5

6 of 32

Wastewater Discharge in the Tijuana River

  • A key source of pollution: Overloaded, unmaintained, or inoperable treatment plants, Tijuana

  • Multidimensional issue:
    • Funding
    • Skilled workforce
    • Engineering challenges
    • International relations

  • Actively changing

6

7 of 32

Many Partners in the Work

  • EPA
  • County of San Diego
  • City of San Diego
  • City of Imperial Beach
  • City of Coronado
  • SD Coastkeeper
  • Community groups
  • Partners in Mexico
  • International Boundary and Water Commission (IBWC)
  • More…

7

PIs at Agua a Través de las Fronteras / Water Across Borders meeting, Tijuana MX.

8 of 32

La Jolla

Mission Bay

Point Loma

Coronado

San Diego Bay

USA�Mexico

EMIT L2A

25 Aug 2024

True color

2% stretch

EMIT L2A

25 Aug 2024

True color

0 to 7% stretch

9 of 32

Presentation Title

9

9/4/20XX

10 of 32

Approach

  1. Field radiometers

  • Sondes

  • Water grabs

10

11 of 32

Field Radiometers

GybeSensor

Led by Nick Tufillaro(Gybe, Inc. and Oregon State University)

11

12 of 32

Field Radiometers

Sensors installed at Pier and Boca Rio sites

12

13 of 32

Field Radiometers

Imperial Beach Pier

13

14 of 32

Field Radiometers

Boca Rio (in estuary)

14

15 of 32

Real Time Camera at Boca Rio

HPWREN Camera at TJRE real-time

       

16 of 32

Sondes

Boca Rio (in estuary)

  • Tryptophan
  • CDOM
  • Chlorophyll
  • Turbidity
  • ORP
  • Temperature
  • pH
  • Depth

16

Realtime data served at: https://biggslab.sdsu.edu/research/

Mendoza, L. M., Mladenov, N., Kinoshita, A. M., Pinongcos, F., Verbyla, M. E., & Gersberg, R. (2020). Fluorescence-based monitoring of anthropogenic pollutant inputs to an urban stream in Southern California, USA. Science of the Total Environment718, 137206.

Fig. 1. Representative EEMs from one replicate experiment with a) 0%, b) 5%, c) 20%, and d) 33% synthetic WW addition to creek water. Representative EEMs also shown for field samples previously collected from Alvarado Creek during a storm event on 6 March 2016 at 2-h increments, as shown in e–f (source: Parsons, 2018. Peak T and Peak C regions are indicated with letters T and C, respectively, in panel a). Discharge values and times shown in bottom right corner. Note the lower maximum intensity value (shown in colorbar) at the start of the storm (e) compared to later times (f–h). Colorbar intensities are in Raman units (RU). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Fig. 2. Comparison of synthetic wastewater (WW) in tap water (a, d, g), synthetic WW in creek water (b, e, h), and raw WW in creek water (c, f, i) with TRP fluorescence (top panels), humic-like and chl a fluorescence intensities (middle panels), and TRP:humic ratios (bottom panels). Error bars represent standard deviations of three independent experiments. P values correspond to the curve fitting. All relationships, with the exception of Chl a intensity vs. % synthetic WW in (e), are significant at the p < 0.05 level. All relationships are linear with the exception of TRP:CDOM fluorescence (g), where the fit is a second order polynomial to the equation y = −0.001x2 + 0.059x + 0.3676.

17 of 32

Water Grabs

17

18 of 32

Linking Chemical Composition of Untreated Wastewater with Laboratory, In Situ, and EMIT Spaceborne Spectroscopy

Eva Scrivner1,2

Natalie Mladenov3, Trent Biggs1, Alexandra Grant3, Elise Piazza1, Stephany Garcia4, Christine M. Lee5, Christiana Ade5, Nick Tufillaro6, Philipp Grötsch6, Omar Zurita6, Benjamin Holt5, Daniel Sousa1

1Department of Geography, San Diego State University, San Diego, CA, USA

2Now at: Department of Marine Sciences, University of Connecticut, Groton, CT

3Department of Civil, Construction, and Environmental Engineering, San Diego State University, San Diego, CA, USA

4Tijuana River National Estuarine Research Reserve and Southwest Wetlands Interpretive Association, USA

5Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, USA

5Gybe, Corvallis, OR, USA

19 of 32

Background on Eva

  • Undergrad CSUMB
  • SARP Student (2022)
  • Post-baccalaureate research assistant (2023 – 2024)
  • Just finished 1st year in Marine Sciences PhD program, Uconn
    • Advisor: Heidi Dierssen
    • Thesis topic: PACE imaging of Antarctic Phytoplankton

20 of 32

Wastewater Addition Experiments

  • Varying dilutions of WW-SW were prepared.

  • Reflectance measurements made using Spectra Vista Corporation™ (SVC) HR-1024i spectroradiometer.

  • Concurrent water quality measurements made with a HORIBA Aqualog® benchtop fluorometer.

20

21 of 32

In lab Rrs,as % WW increases, �620 nm absorption increases

21

Right: Reflectance of matte black vessel

22 of 32

Water quality parameters highly correlated with 620 nm band depth in two temporally disparate (Oct and Feb) water samples.

22

23 of 32

620 nm absorption present� in situ and in EMIT imagery

23

UL: Lab Rrs from 26 October 2023 and 14 February 2024

UR: Field Rrs from GybeSensor on same dates

LL: Spaceborne Rrs from EMIT on a day with known high discharge (25 March 2023)

LR: Multispectral Rrs from Sentinel-2 does not resolve the 620 nm absorption

24 of 32

EMIT captures the signal

Presentation Title

24

  • Fluorescence line height at 650 nm (top) seems to do a better job than band depth at 620 nm (lower right)

25 of 32

Results

620 nm feature:

  1. increases under high wastewater conditions,
  2. has high correlation with water quality parameters,
  3. Present in lab, field and spaceborne spectroscopic imagery

25

26 of 32

Hypothesis: Phycocyanin

26

  • Phycocyanin characteristically absorbs at 620 nm.
  • Accessory pigment in cyanobacteria.
  • Commonly found or even employed in secondary wastewater management.

Absorption spectra of purified phycocyanin. Figure credit: Paswan et al., 2015

620 nm

27 of 32

Future

27

  • Optimize retrieval
  • Collect more field & lab data
    • Integrate low-cost multispectral visible sensors for on-the-spot measurement
  • Synthesize with other sensing modalities
    • Right: Erin Reilly (MS student) -> plume mapping from reduced SAR backscatter
  • Integrate with regional ocean circulation models
    • UCSD/SIO – Pathogen Forecast Model, Plume tracking
      • Bottom images: Colleagues at UCSD/SIO (Feddersen, Giddings, et al.)�https://scripps.ucsd.edu/crossborderpollution

28 of 32

Acknowledgments

  • We would like to thank all undergraduate collaborators who make our field and laboratory sampling efforts possible, including but not limited to: Callie Summerlin, David Penn, Mia Pollasky, Julian Gutierrez, Scotty Dingwall, Blanca Heredia, Trinity Weary, Tate Mckay, and Yzatis Silva.

  • We gratefully acknowledge primary funding from the NASA Remote Sensing of Water Quality Program (Grant #80NSSC22K0907) and the NASA Applications-Oriented Augmentations for Research and Analysis Program (Grant #80NSSC23K1460).

  • DS also acknowledges funding from the USDA NIFA Sustainable Agroecosystems Program (Grant #2022-67019-36397), the USDA AFRI Rapid Response to Extreme Weather Events Across Food and Agricultural Systems Program (Grant #2023-68016-40683), the NASA Land-Cover/Land Use Change Program (Grant #NNH21ZDA001N-LCLUC), the NASA EMIT Science and Applications Team (Grant #80NSSC24K0861), the NASA Commercial Smallsat Data Analysis Program (Grant #80NSSC24K0052), the NASA FireSense Airborne Science Program (Grant #80NSSC24K0145), the California Climate Action Seed Award Program, and the NSF Signals in the Soil Program (Award #2226649).

28

29 of 32

WASI Inverse Simulations

Presentation Title

29

30 of 32

620 nm absorption is robust to noise in hyperspectral, not multispectral resolution

30

31 of 32

765 and 985 nm absorption

31

  • 765 nm absorption consistent with liquid water.

  • 985 nm highly absorbed by atmospheric water vapor and not easily detectable by satellite.

Absorption spectra of pure seawater.

Figure credit: Pegau and Zaneveld, 1993, Mobley e.d., 2022

32 of 32

References

Paswan, M.B., Chudasama, M.M., Mitra, M. et al. Fluorescence Quenching Property of C-Phycocyanin from Spirulina platensis and its Binding Efficacy with Viable Cell Components. J Fluoresc 26, 577–583 (2016). https://doi.org/10.1007/s10895-015-1742-7

Pegau, W. S., & Zaneveld, J. V. (1993). Temperature-dependent absorption of water in the red and near-infrared portions of the spectrum [Electronic version]. Limnology and Oceanography, 38(1), 188-192.

Mobley, C. D. (ed.) (2022) The Oceanic Optics Book. Dartmouth, NS, Canada, International Ocean Colour Coordinating Group (IOCCG), 924pp. DOI: http://dx.doi.org/10.25607/OBP-1710

* References cited in presentation; full reference list in speaker notes.

32