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MASTER'S COURSE OVERVIEW • MODULES 3 & 4

Molecular Reactions, NGS & Microchip Cultivation

An in-depth exploration of electrophoretic blotting, advanced PCR variants, next-generation sequencing, and microfluidics for microbial dark matter.

Focus

Molecular & Microfluidics

Core Practice

PCR & Biofilm Chips

Degree

Master of Biotechnology

AI - assisted content*

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MODULE 3 • TOPIC 6

Electrophoresis & Blotting Hybridization

Agarose & SDS-PAGE Gels

Size-based separation: Resolving nucleic acid fragments and bacteriocin peptides.

Blotting Systems

Southern, Northern & Western: Specific membrane hybridization for DNA, RNA & proteins.

UniProt Bioinformatics

Database mapping: Protein purity evaluation and molecular mass verification.

Source: www.technologynetworks.com

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MODULE 3 • TOPIC 7

Polymerase Chain Reaction (PCR) & Amplification

Classical & qPCR

Live amplification: Quantitative fluorescence tracking and gene copying.

Reverse Transcription

cDNA synthesis: RNA expression profiling in transcriptomic studies.

Specialized Variants

Multiplex & Touchdown: Nested, colony, and high-specificity priming strategies.

LAB Strain Typing

Target identification: Genus-level verification of lactic acid bacteria.

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MODULE 3 • TOPIC 8

Sequencing Generations & Bioinformatic Pipelines

First-Gen Sanger

Dideoxy procedure: Capillary gel reading for targeted sequence validation.

Illumina & Nanopore

High-throughput NGS: Whole-genome synthesis and single-molecule pore sequencing.

antiSMASH Mining

Genome annotation: Secondary metabolite cluster prediction in GenBank.

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MODULE 4 • TOPIC 9

Cultivating Unculturable "Microbial Dark Matter"

The 99% Unculturable Challenge

Microbial dark matter: Overcoming standard laboratory culture limitations.

Ichip & Micromachined Isolation

In situ growth chambers: Discovery of novel antibiotics like Teixobactin.

Droplet Microfluidics

Picoliter emulsions: High-throughput single-cell screening and enzymatic assays.

Source: www.frontiersin.org

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MODULE 4 & PRACTICAL MASTERY

Biofilm Analysis & Master's Outcomes

BiofilmChip Testing

Crystal violet assays & antimicrobial susceptibility.

PCR Identification Lab

Horizontal agarose gel electrophoresis of LAB genera.

Final Competency

Full design & execution of modern biotech research.