Skill Based Subject-I �Diagnostic Microbiology-I

Transport Medium

Presented By

M. Banupriya

Assistant Professor

Department of Microbiology

Dr. R.A.N.M Arts and Science College, Erode

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Introduction & Definition

• Transport media are special media formulated to preserve a specimen and minimize bacterial overgrowth from the time of collection to the time it is received at the laboratory to be processed.
• Depending on the type of organisms suspected in the sample, transport media may vary. However in general, transport media are classified on the basis of physical state as semi solid and liquid and also in basis of their utility as bacterial or viral transport media.
• Transport media contains only buffers and salt instead of containing any nutritional ingredients such as carbon, nitrogen, and organic growth factors so as to prevent microbial multiplication.

Transport Medium

• Introduction & Definition
• 1.  Amies Medium With Charcoal
• 2.  Amies Medium Without Charcoal
• 3.  Cary and Blair Medium
• 4. Stuarts Medium
• 5. Venkatraman Ramakrishnan (VR) medium
• 6. Sach’s Buffered Glycerol Saline
• 7. Viral transport medium (VTM)
• 8. Anaerobic Transport Medium (ATM and ATMSP) and Anaerobic Dental Transport Medium (DTM)

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1.  Amies Medium With Charcoal

• Charcoal helps eliminate metabolic products of bacterial growth, which may be especially useful in the isolation of fastidious organisms like However it is suggested that, some other pathogens like Campylobacter can also survive in such medium.
• Amies Transport Medium with charcoal is used for transportation and preservation of microbiological specimen.

2.  Amies Medium Without Charcoal

• It is used for collecting, transporting and preserving microbiological specimens. It is formulated to maintain the viability of microorganisms without significant increase in growth, being nonnutritive, phosphate buffered and semi-solid.

3.  Cary and Blair Medium

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• Semi-solid, white colored transport medium for faeces that may contain Salmonella, Shigella, Vibrio or Cam­pylobacter. Cary and Blair Transport Medium is used for collecting, transporting and preserving microbiological specimens, particularly those containing Vibrio cholerae.

4.  Stuarts Medium

•  Commonly used for transporting specimens suspected of having gonococci. Also used for transporting Throat, vaginal, wound and skin swabs that may contain fastidious organisms.

5. Venkatraman Ramakrishnan (VR) medium

• VR medium was used to transport feces from suspected cholera patients.

6. Sach’s Buffered Glycerol Saline

• Glycerol saline transport medium is used to preserve Salmonella and Shigella species and Escherichia coli in facial specimens.

7. Viral transport medium (VTM)

• VTM is a solution used to preserve virus specimens after collection so that they can be transported and analysed in a laboratory at a later time.

8. Anaerobic Transport Medium (ATM and ATMSP) and Anaerobic Dental Transport Medium (DTM)

• Anaerobic Transport Medium (ATM and ATMSP) and Anaerobic Dental Transport Medium (DTM) are mineral salt based semi-solid medias with reducing agents. Designed as a holding medium to maintain the viability of microorganisms, both anaerobic and aerobic, through collection, transportation, and shipment of clinical specimens.

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