Spatio-temporal diffusion of Purmorphamine in collagen gel with microfluidic systems
11th Annual COE Graduate Poster Presentation Competition
Cross-Disciplinary Research Area: Healthcare
Student: Marietou Salou Doudou (MS ) Advisor: Dr. Yun Co-advisor: Dr. Sergey
Clinical Problem to address:
- Neurological disorder second disease leading cause to death after cardiovascular diseases
- Tremendous effort have been achieved in order to treat brain related diseases
- The emergence of organoid for instance has made possible the reproduction of some of the part of brain in vitro.
- Organoid: 3D cluster of cell allowed to grow in vitro capable of have similar functions a human brain
Focus of the study
- During organoid formation concentration gradient of morphogen used to initiate differentiation and cell shaping.
- Few morphogen diffusion quantification is available
Significance of morphogen diffusion quantification
- Deeper understanding of cell to microenvironment exchange
- Knowledge of gradient duration
- Computational modeling: time saving and cost effective
Justification of the study
- Pur: organic and anti-inflammatory properties to treat neurological diseases.
- Gel + microfluidic: ideal for diffusion evaluation
- Determine the Diffusion coefficient of PM with Ibidi
- Design a device on COMSOL to simulate the diffusion of PM.
- Fabricate device and repeat diffusion of PM experiment
- Compare predicted and experimental data
Department of Chemical, Biological and Bioengineering A&T State University, Greensboro, NC
Transport of diluted species
- Photolithography
- Wafer Salinization
- PDMS casting
- Plasma treatment
Raman spectroscopy
Linearity experiment
- Spectrum at different concentration collected
- Plot intensity vs concentration
Diffusion experiment
- Gel seeding and polymerization
- Pur injection and data collection
Fig1:Raman Peaks and Calibration curve
Fig3: Change of c at t= 0.1h t=9h t=30h
Fig4: Predicted values vs theoretical values. P-value:0.96, 0.46 and 0.98